Restriction map for
pF1KE2698
(Linear) (Six-base) MAPSORT of: KIEE2698.seq Check: 84 from: 1 to: 2310
KIEE2698.orf
With 182 enzymes: SgfI *
June 29, 2017 17:53 ..
Acc65I G'GTAC_C
Cuts at: 0 38 2310
Size: 38 2272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 427 808 2105 2137 2310
Size: 253 174 381 1297 32 173
Fragments arranged by size:
1297 381 253 174 173 32
AfeI AGC'GCT
Cuts at: 0 2103 2310
Size: 2103 207
AflIII A'CryG_T
Cuts at: 0 1940 2310
Size: 1940 370
AleI CACnn'nnGTG
Cuts at: 0 163 1174 1867 2310
Size: 163 1011 693 443
Fragments arranged by size:
1011 693 443 163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 761 1006 1834 2310
Size: 761 245 828 476
Fragments arranged by size:
828 761 476 245
AlwNI CAG_nnn'CTG
Cuts at: 0 99 361 429 606 1932 2310
Size: 99 262 68 177 1326 378
Fragments arranged by size:
1326 378 262 177 99 68
ApoI r'AATT_y
Cuts at: 0 1060 1147 1219 1642 2310
Size: 1060 87 72 423 668
Fragments arranged by size:
1060 668 423 87 72
BamHI G'GATC_C
Cuts at: 0 1138 2310
Size: 1138 1172
BanI G'GyrC_C
Cuts at: 0 38 320 1810 2310
Size: 38 282 1490 500
Fragments arranged by size:
1490 500 282 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 2125 2310
Size: 2125 185
BbvCI CC'TCA_GC
Cuts at: 0 2238 2310
Size: 2238 72
BclI T'GATC_A
Cuts at: 0 1313 2310
Size: 1313 997
BfrBI ATG'CAT
Cuts at: 0 993 2310
Size: 993 1317
BglI GCCn_nnn'nGGC
Cuts at: 0 364 2310
Size: 364 1946
BglII A'GATC_T
Cuts at: 0 558 2310
Size: 558 1752
Bme1580I G_kGCm'C
Cuts at: 0 1165 2310
Size: 1165 1145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1386 2301 2310
Size: 1386 915 9
Fragments arranged by size:
1386 915 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 451 487 871 934 1790 2310
Size: 64 387 36 384 63 856 520
Fragments arranged by size:
856 520 387 384 64 63 36
Bpu10I CC'TnA_GC
Cuts at: 0 1522 2238 2310
Size: 1522 716 72
Fragments arranged by size:
1522 716 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 520 1333 1812 2310
Size: 304 216 813 479 498
Fragments arranged by size:
813 498 479 304 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 1318 1357 2310
Size: 1318 39 953
Fragments arranged by size:
1318 953 39
BsaAI yAC'GTr
Cuts at: 0 690 2310
Size: 690 1620
BsaHI Gr'CG_yC
Cuts at: 0 1025 1423 1853 2310
Size: 1025 398 430 457
Fragments arranged by size:
1025 457 430 398
BsaWI w'CCGG_w
Cuts at: 0 452 2310
Size: 452 1858
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1788 1818 2310
Size: 1788 30 492
Fragments arranged by size:
1788 492 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 868 931 1315 1527 2053 2310
Size: 727 141 63 384 212 526 257
Fragments arranged by size:
727 526 384 257 212 141 63
BseYI C'CCAG_C
Cuts at: 0 368 2310
Size: 368 1942
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 712 1291 2310
Size: 484 228 579 1019
Fragments arranged by size:
1019 579 484 228
BsiEI CG_ry'CG
Cuts at: 0 907 2310
Size: 907 1403
BsiHKAI G_wGCw'C
Cuts at: 0 1798 2310
Size: 1798 512
BsmI GAATG_Cn'
Cuts at: 0 2044 2310
Size: 2044 266
Bsp1286I G_dGCh'C
Cuts at: 0 1165 1798 2310
Size: 1165 633 512
Fragments arranged by size:
1165 633 512
BspEI T'CCGG_A
Cuts at: 0 452 2310
Size: 452 1858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 2158 2177 2310
Size: 266 1892 19 133
Fragments arranged by size:
1892 266 133 19
BsrBI CCG'CTC
Cuts at: 0 972 1776 1782 2310
Size: 972 804 6 528
Fragments arranged by size:
972 804 528 6
BsrFI r'CCGG_y
Cuts at: 0 902 1001 1021 2310
Size: 902 99 20 1289
Fragments arranged by size:
1289 902 99 20
BsrGI T'GTAC_A
Cuts at: 0 894 2310
Size: 894 1416
BstAPI GCAn_nnn'nTGC
Cuts at: 0 361 426 597 2310
Size: 361 65 171 1713
Fragments arranged by size:
1713 361 171 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1418 1995 2310
Size: 1418 577 315
Fragments arranged by size:
1418 577 315
BstYI r'GATC_y
Cuts at: 0 468 558 1138 1898 2310
Size: 468 90 580 760 412
Fragments arranged by size:
760 580 468 412 90
Bsu36I CC'TnA_GG
Cuts at: 0 1237 2310
Size: 1237 1073
BtgI C'CryG_G
Cuts at: 0 404 1907 2310
Size: 404 1503 403
Fragments arranged by size:
1503 404 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 694 2310
Size: 694 1616
BtsI GCAGTG_nn'
Cuts at: 0 359 620 2310
Size: 359 261 1690
Fragments arranged by size:
1690 359 261
EaeI y'GGCC_r
Cuts at: 0 137 904 1511 2062 2310
Size: 137 767 607 551 248
Fragments arranged by size:
767 607 551 248 137
EagI C'GGCC_G
Cuts at: 0 904 2310
Size: 904 1406
EarI CTCTTCn'nnn_
Cuts at: 0 451 724 2310
Size: 451 273 1586
Fragments arranged by size:
1586 451 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 253 427 451 487 808 871 934
Size: 64 189 174 24 36 321 63 63
Cuts at: 934 1790 2105 2137 2310
Size: 856 315 32 173
Fragments arranged by size:
856 321 315 189 174 173 64 63
63 36 32 24
EcoO109I rG'GnC_Cy
Cuts at: 0 885 1518 1600 2310
Size: 885 633 82 710
Fragments arranged by size:
885 710 633 82
EcoRI G'AATT_C
Cuts at: 0 1219 2310
Size: 1219 1091
HaeII r_GCGC'y
Cuts at: 0 2105 2310
Size: 2105 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 264 296 1096 1128 1440 1472
Size: 102 32 130 32 800 32 312 32
Cuts at: 1472 1547 1579 2310
Size: 75 32 731
Fragments arranged by size:
800 731 312 130 102 75 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 835 2282 2310
Size: 835 1447 28
Fragments arranged by size:
1447 835 28
KpnI G_GTAC'C
Cuts at: 0 42 2310
Size: 42 2268
MfeI C'AATT_G
Cuts at: 0 1272 1490 2310
Size: 1272 218 820
Fragments arranged by size:
1272 820 218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1418 2310
Size: 1220 198 892
Fragments arranged by size:
1220 892 198
MslI CAynn'nnrTG
Cuts at: 0 163 988 1174 1867 2310
Size: 163 825 186 693 443
Fragments arranged by size:
825 693 443 186 163
MspA1I CmG'CkG
Cuts at: 0 606 1572 1886 1932 2310
Size: 606 966 314 46 378
Fragments arranged by size:
966 606 378 314 46
NdeI CA'TA_TG
Cuts at: 0 132 2310
Size: 132 2178
NsiI A_TGCA'T
Cuts at: 0 995 2310
Size: 995 1315
NspI r_CATG'y
Cuts at: 0 157 555 987 1446 1944 2310
Size: 157 398 432 459 498 366
Fragments arranged by size:
498 459 432 398 366 157
PciI A'CATG_T
Cuts at: 0 1940 2310
Size: 1940 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 1433 1908 2310
Size: 1433 475 402
Fragments arranged by size:
1433 475 402
PfoI T'CCnGG_A
Cuts at: 0 503 1141 2310
Size: 503 638 1169
Fragments arranged by size:
1169 638 503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 602 634 2310
Size: 602 32 1676
Fragments arranged by size:
1676 602 32
PpuMI rG'GwC_Cy
Cuts at: 0 885 1518 1600 2310
Size: 885 633 82 710
Fragments arranged by size:
885 710 633 82
PshAI GACnn'nnGTC
Cuts at: 0 1024 2310
Size: 1024 1286
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1732 1764 2310
Size: 1732 32 546
Fragments arranged by size:
1732 546 32
PstI C_TGCA'G
Cuts at: 0 357 1218 2155 2310
Size: 357 861 937 155
Fragments arranged by size:
937 861 357 155
PvuII CAG'CTG
Cuts at: 0 606 1572 1932 2310
Size: 606 966 360 378
Fragments arranged by size:
966 606 378 360
SanDI GG'GwC_CC
Cuts at: 0 885 2310
Size: 885 1425
SexAI A'CCwGG_T
Cuts at: 0 1602 2310
Size: 1602 708
SfcI C'TryA_G
Cuts at: 0 19 63 346 353 1214 2151 2310
Size: 19 44 283 7 861 937 159
Fragments arranged by size:
937 861 283 159 44 19 7
SmlI C'TyrA_G
Cuts at: 0 283 535 1348 1791 2310
Size: 283 252 813 443 519
Fragments arranged by size:
813 519 443 283 252
SphI G_CATG'C
Cuts at: 0 987 2310
Size: 987 1323
StuI AGG'CCT
Cuts at: 0 1346 2310
Size: 1346 964
StyI C'CwwG_G
Cuts at: 0 106 1341 2033 2310
Size: 106 1235 692 277
Fragments arranged by size:
1235 692 277 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1342 1857 2310
Size: 1342 515 453
Fragments arranged by size:
1342 515 453
TatI w'GTAC_w
Cuts at: 0 894 1721 2310
Size: 894 827 589
Fragments arranged by size:
894 827 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1592 1624 1788 1820 2310
Size: 1592 32 164 32 490
Fragments arranged by size:
1592 490 164 32 32
XbaI T'CTAG_A
Cuts at: 0 471 2310
Size: 471 1839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 1496 2310
Size: 402 1094 814
Fragments arranged by size:
1094 814 402
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApoI
BamHI BanI BbsI BbvCI BclI BfrBI BglI BglII
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI EarI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KpnI MfeI
MmeI MslI MspA1I NdeI NsiI NspI PciI PflMI
PfoI PpiI PpuMI PshAI PsrI PstI PvuII SanDI
SexAI SfcI SmlI SphI StuI StyI TaqII TatI
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanII BbeI BcgI BciVI BlpI BmgBI BmrI BmtI
BsaBI BsiWI BsmBI BspHI BsrDI BssHII BssSI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XhoI XmaI XmnI
ZraI