Restriction map for
pF1KE2692
(Linear) (Six-base) MAPSORT of: KIEE2692.seq Check: 9268 from: 1 to: 2178
KIEE2692.orf
With 182 enzymes: SgfI *
June 23, 2017 13:27 ..
Acc65I G'GTAC_C
Cuts at: 0 355 2178
Size: 355 1823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1656 1849 2178
Size: 399 1257 193 329
Fragments arranged by size:
1257 399 329 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1149 2178
Size: 1149 1029
AlwNI CAG_nnn'CTG
Cuts at: 0 796 1753 2014 2178
Size: 796 957 261 164
Fragments arranged by size:
957 796 261 164
ApoI r'AATT_y
Cuts at: 0 736 1434 2178
Size: 736 698 744
Fragments arranged by size:
744 736 698
AscI GG'CGCG_CC
Cuts at: 0 19 2178
Size: 19 2159
AvaI C'yCGr_G
Cuts at: 0 30 2178
Size: 30 2148
BanI G'GyrC_C
Cuts at: 0 48 355 2143 2178
Size: 48 307 1788 35
Fragments arranged by size:
1788 307 48 35
BanII G_rGCy'C
Cuts at: 0 170 329 1634 2178
Size: 170 159 1305 544
Fragments arranged by size:
1305 544 170 159
BbeI G_GCGC'C
Cuts at: 0 52 2178
Size: 52 2126
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 1958 2178
Size: 410 1548 220
Fragments arranged by size:
1548 410 220
BciVI GTATCCnnnnn_n'
Cuts at: 0 580 2178
Size: 580 1598
BclI T'GATC_A
Cuts at: 0 1815 2178
Size: 1815 363
BglI GCCn_nnn'nGGC
Cuts at: 0 60 2178
Size: 60 2118
Bme1580I G_kGCm'C
Cuts at: 0 2146 2178
Size: 2146 32
BmgBI CAC'GTC
Cuts at: 0 831 2178
Size: 831 1347
BmrI ACTGGGnnnn_n'
Cuts at: 0 784 2178
Size: 784 1394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1650 1971 2178
Size: 1650 321 207
Fragments arranged by size:
1650 321 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1128 2077 2119 2178
Size: 1099 29 949 42 59
Fragments arranged by size:
1099 949 59 42 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2013 2178
Size: 2013 165
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 2099 2114 2178
Size: 295 1804 15 64
Fragments arranged by size:
1804 295 64 15
BsaHI Gr'CG_yC
Cuts at: 0 49 1622 2178
Size: 49 1573 556
Fragments arranged by size:
1573 556 49
BsaWI w'CCGG_w
Cuts at: 0 1766 2178
Size: 1766 412
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 848 878 1334 1364 1375 1405 2029 2059
Size: 848 30 456 30 11 30 624 30
Cuts at: 2059 2178
Size: 119
Fragments arranged by size:
848 624 456 119 30 30 30 30
11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 117 178 205 1592 1642 2016 2178
Size: 114 3 61 27 1387 50 374 162
Fragments arranged by size:
1387 374 162 114 61 50 27 3
BseYI C'CCAG_C
Cuts at: 0 84 2178
Size: 84 2094
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 378 1045 2178
Size: 280 98 667 1133
Fragments arranged by size:
1133 667 280 98
BsiEI CG_ry'CG
Cuts at: 0 110 2178
Size: 110 2068
BsiHKAI G_wGCw'C
Cuts at: 0 170 1114 2178
Size: 170 944 1064
Fragments arranged by size:
1064 944 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1075 2178
Size: 1075 1103
Bsp1286I G_dGCh'C
Cuts at: 0 170 329 1114 1634 2146 2178
Size: 170 159 785 520 512 32
Fragments arranged by size:
785 520 512 170 159 32
BspHI T'CATG_A
Cuts at: 0 707 2178
Size: 707 1471
BsrBI CCG'CTC
Cuts at: 0 1389 2178
Size: 1389 789
BsrDI GCAATG_nn'
Cuts at: 0 1481 2178
Size: 1481 697
BsrFI r'CCGG_y
Cuts at: 0 200 2178
Size: 200 1978
BssHII G'CGCG_C
Cuts at: 0 19 2178
Size: 19 2159
BssSI C'ACGA_G
Cuts at: 0 298 2049 2178
Size: 298 1751 129
Fragments arranged by size:
1751 298 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1513 2178
Size: 1513 665
BstBI TT'CG_AA
Cuts at: 0 452 2178
Size: 452 1726
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1362 2055 2178
Size: 1362 693 123
Fragments arranged by size:
1362 693 123
BstYI r'GATC_y
Cuts at: 0 625 834 2178
Size: 625 209 1344
Fragments arranged by size:
1344 625 209
Bsu36I CC'TnA_GG
Cuts at: 0 346 1465 2178
Size: 346 1119 713
Fragments arranged by size:
1119 713 346
BtgI C'CryG_G
Cuts at: 0 58 110 158 1007 2178
Size: 58 52 48 849 1171
Fragments arranged by size:
1171 849 58 52 48
BtsI GCAGTG_nn'
Cuts at: 0 1062 2178
Size: 1062 1116
DraIII CAC_nnn'GTG
Cuts at: 0 1849 2178
Size: 1849 329
EaeI y'GGCC_r
Cuts at: 0 107 311 372 1490 2178
Size: 107 204 61 1118 688
Fragments arranged by size:
1118 688 204 107 61
EagI C'GGCC_G
Cuts at: 0 107 2178
Size: 107 2071
EarI CTCTTCn'nnn_
Cuts at: 0 274 595 1634 2071 2178
Size: 274 321 1039 437 107
Fragments arranged by size:
1039 437 321 274 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 202 2178
Size: 157 45 1976
Fragments arranged by size:
1976 157 45
EcoICRI GAG'CTC
Cuts at: 0 168 2178
Size: 168 2010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1099 1128 1656 1849 2077 2119 2178
Size: 399 700 29 528 193 228 42 59
Fragments arranged by size:
700 528 399 228 193 59 42 29
EcoO109I rG'GnC_Cy
Cuts at: 0 410 2178
Size: 410 1768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 624 1254 2178
Size: 624 630 924
Fragments arranged by size:
924 630 624
HaeII r_GCGC'y
Cuts at: 0 52 158 1090 2178
Size: 52 106 932 1088
Fragments arranged by size:
1088 932 106 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 758 790 848 880 1492 1524 1607 1639
Size: 758 32 58 32 612 32 83 32
Cuts at: 1639 2178
Size: 539
Fragments arranged by size:
758 612 539 83 58 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1686 2178
Size: 1686 492
KasI G'GCGC_C
Cuts at: 0 48 2178
Size: 48 2130
KpnI G_GTAC'C
Cuts at: 0 359 2178
Size: 359 1819
MscI TGG'CCA
Cuts at: 0 313 1492 2178
Size: 313 1179 686
Fragments arranged by size:
1179 686 313
MslI CAynn'nnrTG
Cuts at: 0 1450 2178
Size: 1450 728
MspA1I CmG'CkG
Cuts at: 0 60 78 112 392 440 922 2178
Size: 60 18 34 280 48 482 1256
Fragments arranged by size:
1256 482 280 60 48 34 18
NarI GG'CG_CC
Cuts at: 0 49 2178
Size: 49 2129
NcoI C'CATG_G
Cuts at: 0 158 1007 2178
Size: 158 849 1171
Fragments arranged by size:
1171 849 158
NotI GC'GGCC_GC
Cuts at: 0 107 2178
Size: 107 2071
NspI r_CATG'y
Cuts at: 0 826 2178
Size: 826 1352
PfoI T'CCnGG_A
Cuts at: 0 1105 2178
Size: 1105 1073
PvuII CAG'CTG
Cuts at: 0 392 2178
Size: 392 1786
SacI G_AGCT'C
Cuts at: 0 170 2178
Size: 170 2008
SacII CC_GC'GG
Cuts at: 0 61 113 2178
Size: 61 52 2065
Fragments arranged by size:
2065 61 52
SapI GCTCTTCn'nnn_
Cuts at: 0 274 2071 2178
Size: 274 1797 107
Fragments arranged by size:
1797 274 107
ScaI AGT'ACT
Cuts at: 0 1669 2178
Size: 1669 509
SfoI GGC'GCC
Cuts at: 0 50 2178
Size: 50 2128
SmlI C'TyrA_G
Cuts at: 0 2028 2178
Size: 2028 150
SphI G_CATG'C
Cuts at: 0 826 2178
Size: 826 1352
StuI AGG'CCT
Cuts at: 0 1463 2178
Size: 1463 715
StyI C'CwwG_G
Cuts at: 0 158 1007 2178
Size: 158 849 1171
Fragments arranged by size:
1171 849 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1605 1722 1987 2178
Size: 1605 117 265 191
Fragments arranged by size:
1605 265 191 117
TatI w'GTAC_w
Cuts at: 0 888 1667 2178
Size: 888 779 511
Fragments arranged by size:
888 779 511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2029 2061 2178
Size: 2029 32 117
Fragments arranged by size:
2029 117 32
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApoI AscI AvaI BanI
BanII BbeI BbsI BciVI BclI BglI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
KasI KpnI MscI MslI MspA1I NarI NcoI NotI
NspI PfoI PvuII SacI SacII SapI ScaI SfoI
SmlI SphI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BfrBI BglII BlpI BmtI
Bpu10I BsaAI BsaBI BsiWI BsmI BspEI BspMI BsrGI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SanDI SbfI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI