Restriction map for
pF1KE2691
(Linear) (Six-base) MAPSORT of: KIEE2691.seq Check: 3272 from: 1 to: 2445
KIEE2691.orf
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AatII G_ACGT'C
Cuts at: 0 479 2445
Size: 479 1966
AccI GT'mk_AC
Cuts at: 0 932 1508 2445
Size: 932 576 937
Fragments arranged by size:
937 932 576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 2230 2445
Size: 1168 1062 215
Fragments arranged by size:
1168 1062 215
AfeI AGC'GCT
Cuts at: 0 1546 1699 2377 2445
Size: 1546 153 678 68
Fragments arranged by size:
1546 678 153 68
AflIII A'CryG_T
Cuts at: 0 291 852 2445
Size: 291 561 1593
Fragments arranged by size:
1593 561 291
AgeI A'CCGG_T
Cuts at: 0 1652 2445
Size: 1652 793
AleI CACnn'nnGTG
Cuts at: 0 292 1960 2445
Size: 292 1668 485
Fragments arranged by size:
1668 485 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2305 2445
Size: 2305 140
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2414 2445
Size: 2414 31
AlwNI CAG_nnn'CTG
Cuts at: 0 1578 1935 1998 2445
Size: 1578 357 63 447
Fragments arranged by size:
1578 447 357 63
ApaI G_GGCC'C
Cuts at: 0 2097 2445
Size: 2097 348
ApoI r'AATT_y
Cuts at: 0 743 2445
Size: 743 1702
AvaI C'yCGr_G
Cuts at: 0 2090 2179 2445
Size: 2090 89 266
Fragments arranged by size:
2090 266 89
BamHI G'GATC_C
Cuts at: 0 323 2238 2445
Size: 323 1915 207
Fragments arranged by size:
1915 323 207
BanI G'GyrC_C
Cuts at: 0 559 2055 2445
Size: 559 1496 390
Fragments arranged by size:
1496 559 390
BanII G_rGCy'C
Cuts at: 0 1040 1712 2097 2445
Size: 1040 672 385 348
Fragments arranged by size:
1040 672 385 348
BbsI GAAGACnn'nnnn_
Cuts at: 0 471 993 1610 1927 2322 2445
Size: 471 522 617 317 395 123
Fragments arranged by size:
617 522 471 395 317 123
BbvCI CC'TCA_GC
Cuts at: 0 2255 2445
Size: 2255 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 288 322 2445
Size: 288 34 2123
Fragments arranged by size:
2123 288 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2363 2445
Size: 2363 82
BclI T'GATC_A
Cuts at: 0 237 434 2445
Size: 237 197 2011
Fragments arranged by size:
2011 237 197
BglI GCCn_nnn'nGGC
Cuts at: 0 537 641 2445
Size: 537 104 1804
Fragments arranged by size:
1804 537 104
BglII A'GATC_T
Cuts at: 0 311 2445
Size: 311 2134
BlpI GC'TnA_GC
Cuts at: 0 115 2445
Size: 115 2330
Bme1580I G_kGCm'C
Cuts at: 0 562 589 2097 2445
Size: 562 27 1508 348
Fragments arranged by size:
1508 562 348 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 2339 2445
Size: 2339 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 2047 2445
Size: 94 1953 398
Fragments arranged by size:
1953 398 94
Bpu10I CC'TnA_GC
Cuts at: 0 886 2255 2405 2445
Size: 886 1369 150 40
Fragments arranged by size:
1369 886 150 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 2445
Size: 360 2085
BsaI GGTCTCn'nnnn_
Cuts at: 0 1165 2445
Size: 1165 1280
BsaAI yAC'GTr
Cuts at: 0 294 855 2445
Size: 294 561 1590
Fragments arranged by size:
1590 561 294
BsaHI Gr'CG_yC
Cuts at: 0 476 800 1196 2445
Size: 476 324 396 1249
Fragments arranged by size:
1249 476 396 324
BsaWI w'CCGG_w
Cuts at: 0 192 1652 2003 2445
Size: 192 1460 351 442
Fragments arranged by size:
1460 442 351 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 171 201 2293 2323 2445
Size: 171 30 2092 30 122
Fragments arranged by size:
2092 171 122 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 847 1897 1900 2218 2272 2445
Size: 847 1050 3 318 54 173
Fragments arranged by size:
1050 847 318 173 54 3
BseYI C'CCAG_C
Cuts at: 0 1278 1287 1712 2356 2445
Size: 1278 9 425 644 89
Fragments arranged by size:
1278 644 425 89 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1501 2445
Size: 748 753 944
Fragments arranged by size:
944 753 748
BsiEI CG_ry'CG
Cuts at: 0 707 2445
Size: 707 1738
BsiHKAI G_wGCw'C
Cuts at: 0 1040 2445
Size: 1040 1405
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1203 1531 1806 1815 2445
Size: 1203 328 275 9 630
Fragments arranged by size:
1203 630 328 275 9
Bsp1286I G_dGCh'C
Cuts at: 0 562 589 1040 1712 2097 2445
Size: 562 27 451 672 385 348
Fragments arranged by size:
672 562 451 385 348 27
BspEI T'CCGG_A
Cuts at: 0 2003 2445
Size: 2003 442
BspHI T'CATG_A
Cuts at: 0 341 2285 2445
Size: 341 1944 160
Fragments arranged by size:
1944 341 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 2147 2445
Size: 689 1458 298
Fragments arranged by size:
1458 689 298
BsrBI CCG'CTC
Cuts at: 0 187 993 1772 2445
Size: 187 806 779 673
Fragments arranged by size:
806 779 673 187
BsrDI GCAATG_nn'
Cuts at: 0 1718 2445
Size: 1718 727
BsrFI r'CCGG_y
Cuts at: 0 1210 1652 2445
Size: 1210 442 793
Fragments arranged by size:
1210 793 442
BssSI C'ACGA_G
Cuts at: 0 58 155 2445
Size: 58 97 2290
Fragments arranged by size:
2290 97 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1935 2445
Size: 1935 510
BstBI TT'CG_AA
Cuts at: 0 138 2445
Size: 138 2307
BstEII G'GTnAC_C
Cuts at: 0 198 1624 2445
Size: 198 1426 821
Fragments arranged by size:
1426 821 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1761 2445
Size: 510 1251 684
Fragments arranged by size:
1251 684 510
BstYI r'GATC_y
Cuts at: 0 41 311 323 1562 1787 1889 2238 2445
Size: 41 270 12 1239 225 102 349 207
Fragments arranged by size:
1239 349 270 225 207 102 41 12
Bsu36I CC'TnA_GG
Cuts at: 0 1074 1903 2445
Size: 1074 829 542
Fragments arranged by size:
1074 829 542
BtgI C'CryG_G
Cuts at: 0 102 578 707 716 1202 2445
Size: 102 476 129 9 486 1243
Fragments arranged by size:
1243 486 476 129 102 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 1251 1338 1365 2445
Size: 927 324 87 27 1080
Fragments arranged by size:
1080 927 324 87 27
BtsI GCAGTG_nn'
Cuts at: 0 532 1442 2445
Size: 532 910 1003
Fragments arranged by size:
1003 910 532
DraIII CAC_nnn'GTG
Cuts at: 0 593 2445
Size: 593 1852
DrdI GACnn_nn'nnGTC
Cuts at: 0 2143 2445
Size: 2143 302
EaeI y'GGCC_r
Cuts at: 0 704 1208 1614 2445
Size: 704 504 406 831
Fragments arranged by size:
831 704 504 406
EagI C'GGCC_G
Cuts at: 0 704 2445
Size: 704 1741
EarI CTCTTCn'nnn_
Cuts at: 0 493 691 1000 1045 1261 1553 2445
Size: 493 198 309 45 216 292 892
Fragments arranged by size:
892 493 309 292 216 198 45
EcoICRI GAG'CTC
Cuts at: 0 1038 2445
Size: 1038 1407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1168 2047 2230 2445
Size: 94 1074 879 183 215
Fragments arranged by size:
1074 879 215 183 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 829 2445
Size: 436 393 1616
Fragments arranged by size:
1616 436 393
EcoO109I rG'GnC_Cy
Cuts at: 0 429 1365 2136 2445
Size: 429 936 771 309
Fragments arranged by size:
936 771 429 309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1434 2445
Size: 1434 1011
FspI TGC'GCA
Cuts at: 0 298 2445
Size: 298 2147
FspAI rTGC'GCAy
Cuts at: 0 298 2445
Size: 298 2147
HaeII r_GCGC'y
Cuts at: 0 1066 1548 1701 2379 2445
Size: 1066 482 153 678 66
Fragments arranged by size:
1066 678 482 153 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 2223 2255 2293 2325 2445
Size: 1364 32 827 32 38 32 120
Fragments arranged by size:
1364 827 120 38 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 2445
Size: 497 1948
MscI TGG'CCA
Cuts at: 0 1616 2445
Size: 1616 829
MslI CAynn'nnrTG
Cuts at: 0 292 752 1960 2287 2445
Size: 292 460 1208 327 158
Fragments arranged by size:
1208 460 327 292 158
MspA1I CmG'CkG
Cuts at: 0 1932 2025 2445
Size: 1932 93 420
Fragments arranged by size:
1932 420 93
NaeI GCC'GGC
Cuts at: 0 1212 2445
Size: 1212 1233
NcoI C'CATG_G
Cuts at: 0 102 2445
Size: 102 2343
NgoMIV G'CCGG_C
Cuts at: 0 1210 2445
Size: 1210 1235
PasI CC'CwG_GG
Cuts at: 0 906 2445
Size: 906 1539
PflMI CCAn_nnn'nTGG
Cuts at: 0 1737 2070 2445
Size: 1737 333 375
Fragments arranged by size:
1737 375 333
PfoI T'CCnGG_A
Cuts at: 0 386 509 671 1403 1577 2445
Size: 386 123 162 732 174 868
Fragments arranged by size:
868 732 386 174 162 123
PmlI CAC'GTG
Cuts at: 0 294 855 2445
Size: 294 561 1590
Fragments arranged by size:
1590 561 294
PpuMI rG'GwC_Cy
Cuts at: 0 2136 2445
Size: 2136 309
PshAI GACnn'nnGTC
Cuts at: 0 1237 2445
Size: 1237 1208
PspOMI G'GGCC_C
Cuts at: 0 2093 2445
Size: 2093 352
PstI C_TGCA'G
Cuts at: 0 470 2445
Size: 470 1975
PvuII CAG'CTG
Cuts at: 0 2025 2445
Size: 2025 420
SacI G_AGCT'C
Cuts at: 0 1040 2445
Size: 1040 1405
SapI GCTCTTCn'nnn_
Cuts at: 0 691 1000 1045 1261 2445
Size: 691 309 45 216 1184
Fragments arranged by size:
1184 691 309 216 45
SexAI A'CCwGG_T
Cuts at: 0 1297 2192 2445
Size: 1297 895 253
Fragments arranged by size:
1297 895 253
SfcI C'TryA_G
Cuts at: 0 466 1008 2445
Size: 466 542 1437
Fragments arranged by size:
1437 542 466
SmlI C'TyrA_G
Cuts at: 0 375 2445
Size: 375 2070
StuI AGG'CCT
Cuts at: 0 262 2445
Size: 262 2183
StyI C'CwwG_G
Cuts at: 0 102 1337 1703 1871 2435 2445
Size: 102 1235 366 168 564 10
Fragments arranged by size:
1235 564 366 168 102 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2094 2445
Size: 2094 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 171 203 446 478 1819 1851 2445
Size: 171 32 243 32 1341 32 594
Fragments arranged by size:
1341 594 243 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 802 2307 2326 2445
Size: 802 1505 19 119
Fragments arranged by size:
1505 802 119 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1500 1761 2445
Size: 1500 261 684
Fragments arranged by size:
1500 684 261
XmnI GAAnn'nnTTC
Cuts at: 0 601 1417 2445
Size: 601 816 1028
Fragments arranged by size:
1028 816 601
ZraI GAC'GTC
Cuts at: 0 477 2445
Size: 477 1968
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SexAI SfcI SmlI StuI StyI TaqII TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AhdI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BfrBI BmgBI BmtI BplI
BsaBI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
DraI EciI EcoRI EcoRV FseI HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XhoI XmaI