Restriction map for
pF1KE2689
(Linear) (Six-base) MAPSORT of: KIEE2689.seq Check: 750 from: 1 to: 1686
KIEE2689.orf
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 255 976 1686
Size: 255 721 710
Fragments arranged by size:
721 710 255
AatII G_ACGT'C
Cuts at: 0 820 1574 1686
Size: 820 754 112
Fragments arranged by size:
820 754 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1686
Size: 742 944
AflIII A'CryG_T
Cuts at: 0 354 686 1686
Size: 354 332 1000
Fragments arranged by size:
1000 354 332
AhdI GACnn_n'nnGTC
Cuts at: 0 816 1686
Size: 816 870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 856 1686
Size: 856 830
AlwNI CAG_nnn'CTG
Cuts at: 0 270 1144 1686
Size: 270 874 542
Fragments arranged by size:
874 542 270
ApaI G_GGCC'C
Cuts at: 0 59 532 1686
Size: 59 473 1154
Fragments arranged by size:
1154 473 59
ApaLI G'TGCA_C
Cuts at: 0 1084 1686
Size: 1084 602
AvaI C'yCGr_G
Cuts at: 0 505 696 1658 1686
Size: 505 191 962 28
Fragments arranged by size:
962 505 191 28
BanI G'GyrC_C
Cuts at: 0 223 301 510 964 1686
Size: 223 78 209 454 722
Fragments arranged by size:
722 454 223 209 78
BanII G_rGCy'C
Cuts at: 0 59 532 554 1394 1460 1686
Size: 59 473 22 840 66 226
Fragments arranged by size:
840 473 226 66 59 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 730 1686
Size: 654 76 956
Fragments arranged by size:
956 654 76
BbvCI CC'TCA_GC
Cuts at: 0 343 1257 1558 1686
Size: 343 914 301 128
Fragments arranged by size:
914 343 301 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1493 1527 1686
Size: 1493 34 159
Fragments arranged by size:
1493 159 34
BglI GCCn_nnn'nGGC
Cuts at: 0 400 1604 1686
Size: 400 1204 82
Fragments arranged by size:
1204 400 82
BglII A'GATC_T
Cuts at: 0 11 1686
Size: 11 1675
BlpI GC'TnA_GC
Cuts at: 0 445 1686
Size: 445 1241
Bme1580I G_kGCm'C
Cuts at: 0 59 515 532 967 1088 1686
Size: 59 456 17 435 121 598
Fragments arranged by size:
598 456 435 121 59 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 307 1686
Size: 307 1379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 1686
Size: 558 1128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 616 1100 1474 1686
Size: 59 557 484 374 212
Fragments arranged by size:
557 484 374 212 59
Bpu10I CC'TnA_GC
Cuts at: 0 343 1257 1558 1686
Size: 343 914 301 128
Fragments arranged by size:
914 343 301 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 1348 1686
Size: 1348 338
BsaAI yAC'GTr
Cuts at: 0 357 1686
Size: 357 1329
BsaHI Gr'CG_yC
Cuts at: 0 817 1571 1686
Size: 817 754 115
Fragments arranged by size:
817 754 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 1686
Size: 565 30 1091
Fragments arranged by size:
1091 565 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 136 1686
Size: 24 112 1550
Fragments arranged by size:
1550 112 24
BseYI C'CCAG_C
Cuts at: 0 440 480 882 1246 1686
Size: 440 40 402 364 440
Fragments arranged by size:
440 440 402 364 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 547 862 1157 1686
Size: 195 352 315 295 529
Fragments arranged by size:
529 352 315 295 195
BsiEI CG_ry'CG
Cuts at: 0 715 1686
Size: 715 971
BsiHKAI G_wGCw'C
Cuts at: 0 1088 1460 1686
Size: 1088 372 226
Fragments arranged by size:
1088 372 226
BsmI GAATG_Cn'
Cuts at: 0 174 1686
Size: 174 1512
BsmBI CGTCTCn'nnnn_
Cuts at: 0 808 1039 1686
Size: 808 231 647
Fragments arranged by size:
808 647 231
Bsp1286I G_dGCh'C
Cuts at: 0 59 515 532 554 967 1088 1394 1460
Size: 59 456 17 22 413 121 306 66
Cuts at: 1460 1686
Size: 226
Fragments arranged by size:
456 413 306 226 121 66 59 22
17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 255 569 797 956 976 1686
Size: 255 314 228 159 20 710
Fragments arranged by size:
710 314 255 228 159 20
BsrDI GCAATG_nn'
Cuts at: 0 753 1686
Size: 753 933
BsrFI r'CCGG_y
Cuts at: 0 590 1686
Size: 590 1096
BstAPI GCAn_nnn'nTGC
Cuts at: 0 301 1144 1686
Size: 301 843 542
Fragments arranged by size:
843 542 301
BstBI TT'CG_AA
Cuts at: 0 824 1686
Size: 824 862
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 390 1269 1686
Size: 189 201 879 417
Fragments arranged by size:
879 417 201 189
BstYI r'GATC_y
Cuts at: 0 11 845 1140 1241 1686
Size: 11 834 295 101 445
Fragments arranged by size:
834 445 295 101 11
Bsu36I CC'TnA_GG
Cuts at: 0 533 1686
Size: 533 1153
BtgI C'CryG_G
Cuts at: 0 182 219 383 715 983 1232 1686
Size: 182 37 164 332 268 249 454
Fragments arranged by size:
454 332 268 249 182 164 37
BtsI GCAGTG_nn'
Cuts at: 0 155 1022 1542 1686
Size: 155 867 520 144
Fragments arranged by size:
867 520 155 144
DraIII CAC_nnn'GTG
Cuts at: 0 301 1686
Size: 301 1385
EaeI y'GGCC_r
Cuts at: 0 377 712 1271 1686
Size: 377 335 559 415
Fragments arranged by size:
559 415 377 335
EagI C'GGCC_G
Cuts at: 0 712 1686
Size: 712 974
EarI CTCTTCn'nnn_
Cuts at: 0 113 125 1686
Size: 113 12 1561
Fragments arranged by size:
1561 113 12
EcoICRI GAG'CTC
Cuts at: 0 1458 1686
Size: 1458 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 616 742 1100 1474 1686
Size: 59 557 126 358 374 212
Fragments arranged by size:
557 374 358 212 126 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1686
Size: 313 1373
EcoO109I rG'GnC_Cy
Cuts at: 0 55 56 76 529 631 1686
Size: 55 1 20 453 102 1055
Fragments arranged by size:
1055 453 102 55 20 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 837 869 1686
Size: 802 32 3 32 817
Fragments arranged by size:
817 802 32 32 3
MscI TGG'CCA
Cuts at: 0 379 1273 1686
Size: 379 894 413
Fragments arranged by size:
894 413 379
MslI CAynn'nnrTG
Cuts at: 0 238 838 1318 1686
Size: 238 600 480 368
Fragments arranged by size:
600 480 368 238
MspA1I CmG'CkG
Cuts at: 0 209 717 882 936 1686
Size: 209 508 165 54 750
Fragments arranged by size:
750 508 209 165 54
NcoI C'CATG_G
Cuts at: 0 182 219 983 1232 1686
Size: 182 37 764 249 454
Fragments arranged by size:
764 454 249 182 37
NotI GC'GGCC_GC
Cuts at: 0 712 1686
Size: 712 974
NspI r_CATG'y
Cuts at: 0 606 1092 1686
Size: 606 486 594
Fragments arranged by size:
606 594 486
PasI CC'CwG_GG
Cuts at: 0 52 959 1686
Size: 52 907 727
Fragments arranged by size:
907 727 52
PflMI CCAn_nnn'nTGG
Cuts at: 0 594 1686
Size: 594 1092
PmlI CAC'GTG
Cuts at: 0 357 1686
Size: 357 1329
PpuMI rG'GwC_Cy
Cuts at: 0 76 631 1686
Size: 76 555 1055
Fragments arranged by size:
1055 555 76
PspOMI G'GGCC_C
Cuts at: 0 55 528 1686
Size: 55 473 1158
Fragments arranged by size:
1158 473 55
PvuII CAG'CTG
Cuts at: 0 209 882 1686
Size: 209 673 804
Fragments arranged by size:
804 673 209
SacI G_AGCT'C
Cuts at: 0 1460 1686
Size: 1460 226
SacII CC_GC'GG
Cuts at: 0 718 1686
Size: 718 968
SanDI GG'GwC_CC
Cuts at: 0 76 631 1686
Size: 76 555 1055
Fragments arranged by size:
1055 555 76
ScaI AGT'ACT
Cuts at: 0 1180 1686
Size: 1180 506
SexAI A'CCwGG_T
Cuts at: 0 1055 1686
Size: 1055 631
SfcI C'TryA_G
Cuts at: 0 1014 1686
Size: 1014 672
SmaI CCC'GGG
Cuts at: 0 507 1686
Size: 507 1179
StuI AGG'CCT
Cuts at: 0 538 1375 1686
Size: 538 837 311
Fragments arranged by size:
837 538 311
StyI C'CwwG_G
Cuts at: 0 59 182 219 259 624 983 1232 1686
Size: 59 123 37 40 365 359 249 454
Fragments arranged by size:
454 365 359 249 123 59 40 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 289 1387 1686
Size: 195 94 1098 299
Fragments arranged by size:
1098 299 195 94
TatI w'GTAC_w
Cuts at: 0 1111 1124 1178 1686
Size: 1111 13 54 508
Fragments arranged by size:
1111 508 54 13
Tth111I GACn'n_nGTC
Cuts at: 0 1570 1686
Size: 1570 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 387 483 886 1686
Size: 189 198 96 403 800
Fragments arranged by size:
800 403 198 189 96
XmaI C'CCGG_G
Cuts at: 0 505 1686
Size: 505 1181
ZraI GAC'GTC
Cuts at: 0 818 1572 1686
Size: 818 754 114
Fragments arranged by size:
818 754 114
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MscI MslI
MspA1I NcoI NotI NspI PasI PflMI PmlI PpuMI
PspOMI PvuII SacI SacII SanDI ScaI SexAI SfcI
SmaI StuI StyI TaqII TatI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BclI BfrBI BmgBI BmtI BpuEI BsaBI BsaWI
BsiWI BspEI BspHI BsrBI BsrGI BssHII BssSI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NruI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SalI SapI SbfI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI XmnI