Restriction map for
pF1KE2680
(Linear) (Six-base) MAPSORT of: KIEE2680.seq Check: 5059 from: 1 to: 1281
KIEE2680.orf
With 182 enzymes: SgfI *
March 1, 2017 16:47 ..
AatII G_ACGT'C
Cuts at: 0 251 1205 1281
Size: 251 954 76
Fragments arranged by size:
954 251 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 534 1281
Size: 172 362 747
Fragments arranged by size:
747 362 172
AfeI AGC'GCT
Cuts at: 0 400 892 1281
Size: 400 492 389
Fragments arranged by size:
492 400 389
AgeI A'CCGG_T
Cuts at: 0 1211 1281
Size: 1211 70
AleI CACnn'nnGTG
Cuts at: 0 1165 1281
Size: 1165 116
AvaI C'yCGr_G
Cuts at: 0 513 1124 1281
Size: 513 611 157
Fragments arranged by size:
611 513 157
BamHI G'GATC_C
Cuts at: 0 106 729 1013 1281
Size: 106 623 284 268
Fragments arranged by size:
623 284 268 106
BanI G'GyrC_C
Cuts at: 0 1141 1281
Size: 1141 140
BanII G_rGCy'C
Cuts at: 0 56 164 1281
Size: 56 108 1117
Fragments arranged by size:
1117 108 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 282 1281
Size: 282 999
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1165 1199 1281
Size: 1165 34 82
Fragments arranged by size:
1165 82 34
BglII A'GATC_T
Cuts at: 0 635 1281
Size: 635 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 871 1281
Size: 622 249 410
Fragments arranged by size:
622 410 249
Bpu10I CC'TnA_GC
Cuts at: 0 688 1281
Size: 688 593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1281
Size: 50 1231
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 751 1281
Size: 454 297 530
Fragments arranged by size:
530 454 297
BsaBI GATnn'nnATC
Cuts at: 0 1051 1281
Size: 1051 230
BsaHI Gr'CG_yC
Cuts at: 0 248 1202 1281
Size: 248 954 79
Fragments arranged by size:
954 248 79
BsaWI w'CCGG_w
Cuts at: 0 1211 1281
Size: 1211 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 66 96 961 991 1281
Size: 66 30 865 30 290
Fragments arranged by size:
865 290 66 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 904 982 1281
Size: 53 851 78 299
Fragments arranged by size:
851 299 78 53
BseYI C'CCAG_C
Cuts at: 0 418 908 1281
Size: 418 490 373
Fragments arranged by size:
490 418 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 732 757 907 1096 1281
Size: 649 83 25 150 189 185
Fragments arranged by size:
649 189 185 150 83 25
BsiHKAI G_wGCw'C
Cuts at: 0 76 164 1281
Size: 76 88 1117
Fragments arranged by size:
1117 88 76
BsmI GAATG_Cn'
Cuts at: 0 221 1281
Size: 221 1060
Bsp1286I G_dGCh'C
Cuts at: 0 56 76 164 1281
Size: 56 20 88 1117
Fragments arranged by size:
1117 88 56 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1118 1281
Size: 1118 163
BsrFI r'CCGG_y
Cuts at: 0 1211 1281
Size: 1211 70
BssSI C'ACGA_G
Cuts at: 0 360 532 655 916 1281
Size: 360 172 123 261 365
Fragments arranged by size:
365 360 261 172 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 576 780 1281
Size: 576 204 501
Fragments arranged by size:
576 501 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 130 1281
Size: 130 1151
BstYI r'GATC_y
Cuts at: 0 106 635 729 1013 1281
Size: 106 529 94 284 268
Fragments arranged by size:
529 284 268 106 94
Bsu36I CC'TnA_GG
Cuts at: 0 1091 1281
Size: 1091 190
BtgI C'CryG_G
Cuts at: 0 734 1281
Size: 734 547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 924 1281
Size: 924 357
BtsI GCAGTG_nn'
Cuts at: 0 670 1281
Size: 670 611
DraIII CAC_nnn'GTG
Cuts at: 0 576 1119 1281
Size: 576 543 162
Fragments arranged by size:
576 543 162
EaeI y'GGCC_r
Cuts at: 0 299 935 1022 1038 1281
Size: 299 636 87 16 243
Fragments arranged by size:
636 299 243 87 16
EarI CTCTTCn'nnn_
Cuts at: 0 38 44 1281
Size: 38 6 1237
Fragments arranged by size:
1237 38 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 1026 1281
Size: 631 395 255
Fragments arranged by size:
631 395 255
EcoICRI GAG'CTC
Cuts at: 0 162 1281
Size: 162 1119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 534 622 871 1281
Size: 172 362 88 249 410
Fragments arranged by size:
410 362 249 172 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 691 1281
Size: 256 435 590
Fragments arranged by size:
590 435 256
EcoO109I rG'GnC_Cy
Cuts at: 0 509 1281
Size: 509 772
HaeII r_GCGC'y
Cuts at: 0 402 532 683 894 975 1281
Size: 402 130 151 211 81 306
Fragments arranged by size:
402 306 211 151 130 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 961 993 1052 1084 1281
Size: 961 32 59 32 197
Fragments arranged by size:
961 197 59 32 32
HincII GTy'rAC
Cuts at: 0 327 1065 1281
Size: 327 738 216
Fragments arranged by size:
738 327 216
MscI TGG'CCA
Cuts at: 0 301 1024 1281
Size: 301 723 257
Fragments arranged by size:
723 301 257
MslI CAynn'nnrTG
Cuts at: 0 739 1165 1281
Size: 739 426 116
Fragments arranged by size:
739 426 116
MspA1I CmG'CkG
Cuts at: 0 286 609 1008 1281
Size: 286 323 399 273
Fragments arranged by size:
399 323 286 273
NcoI C'CATG_G
Cuts at: 0 734 1281
Size: 734 547
NspI r_CATG'y
Cuts at: 0 417 618 1089 1281
Size: 417 201 471 192
Fragments arranged by size:
471 417 201 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 1281
Size: 501 780
PfoI T'CCnGG_A
Cuts at: 0 137 268 1205 1281
Size: 137 131 937 76
Fragments arranged by size:
937 137 131 76
PpuMI rG'GwC_Cy
Cuts at: 0 509 1281
Size: 509 772
PshAI GACnn'nnGTC
Cuts at: 0 286 1281
Size: 286 995
PstI C_TGCA'G
Cuts at: 0 785 1281
Size: 785 496
PvuII CAG'CTG
Cuts at: 0 609 1281
Size: 609 672
SacI G_AGCT'C
Cuts at: 0 164 1281
Size: 164 1117
SanDI GG'GwC_CC
Cuts at: 0 509 1281
Size: 509 772
SapI GCTCTTCn'nnn_
Cuts at: 0 44 1281
Size: 44 1237
ScaI AGT'ACT
Cuts at: 0 790 1281
Size: 790 491
SexAI A'CCwGG_T
Cuts at: 0 860 1281
Size: 860 421
SfcI C'TryA_G
Cuts at: 0 307 781 1281
Size: 307 474 500
Fragments arranged by size:
500 474 307
SmaI CCC'GGG
Cuts at: 0 515 1126 1281
Size: 515 611 155
Fragments arranged by size:
611 515 155
SmlI C'TyrA_G
Cuts at: 0 65 1281
Size: 65 1216
SrfI GCCC'GGGC
Cuts at: 0 1126 1281
Size: 1126 155
StuI AGG'CCT
Cuts at: 0 1151 1281
Size: 1151 130
StyI C'CwwG_G
Cuts at: 0 734 1281
Size: 734 547
TatI w'GTAC_w
Cuts at: 0 410 788 1281
Size: 410 378 493
Fragments arranged by size:
493 410 378
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 66 98 238 270 1281
Size: 66 32 140 32 1011
Fragments arranged by size:
1011 140 66 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 240 270 1281
Size: 240 30 1011
Fragments arranged by size:
1011 240 30
XmaI C'CCGG_G
Cuts at: 0 513 1124 1281
Size: 513 611 157
Fragments arranged by size:
611 513 157
ZraI GAC'GTC
Cuts at: 0 249 1203 1281
Size: 249 954 78
Fragments arranged by size:
954 249 78
Enzymes that do cut:
AatII AcuI AfeI AgeI AleI AvaI BamHI BanI
BanII BbsI BcgI BglII BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MscI
MslI MspA1I NcoI NspI PflMI PfoI PpuMI PshAI
PstI PvuII SacI SanDI SapI ScaI SexAI SfcI
SmaI SmlI SrfI StuI StyI TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BsaAI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TaqII Tth111I XbaI
XhoI XmnI