Restriction map for
pF1KE2674
(Linear) (Six-base) MAPSORT of: KIEE2674.seq Check: 9791 from: 1 to: 2943
KIEE2674.orf
With 182 enzymes: SgfI *
February 27, 2017 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1833 1955 2943
Size: 1833 122 988
Fragments arranged by size:
1833 988 122
Acc65I G'GTAC_C
Cuts at: 0 2059 2943
Size: 2059 884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 2750 2943
Size: 185 2565 193
Fragments arranged by size:
2565 193 185
AflIII A'CryG_T
Cuts at: 0 1825 2943
Size: 1825 1118
AleI CACnn'nnGTG
Cuts at: 0 1939 2943
Size: 1939 1004
ApaLI G'TGCA_C
Cuts at: 0 2148 2943
Size: 2148 795
ApoI r'AATT_y
Cuts at: 0 419 558 1343 1369 2943
Size: 419 139 785 26 1574
Fragments arranged by size:
1574 785 419 139 26
AseI AT'TA_AT
Cuts at: 0 610 2943
Size: 610 2333
AvaI C'yCGr_G
Cuts at: 0 221 2217 2943
Size: 221 1996 726
Fragments arranged by size:
1996 726 221
BanI G'GyrC_C
Cuts at: 0 2059 2943
Size: 2059 884
BanII G_rGCy'C
Cuts at: 0 1466 2943
Size: 1466 1477
BbsI GAAGACnn'nnnn_
Cuts at: 0 2810 2943
Size: 2810 133
BciVI GTATCCnnnnn_n'
Cuts at: 0 2462 2625 2943
Size: 2462 163 318
Fragments arranged by size:
2462 318 163
BclI T'GATC_A
Cuts at: 0 405 1647 2943
Size: 405 1242 1296
Fragments arranged by size:
1296 1242 405
BglII A'GATC_T
Cuts at: 0 1995 2943
Size: 1995 948
Bme1580I G_kGCm'C
Cuts at: 0 2152 2496 2943
Size: 2152 344 447
Fragments arranged by size:
2152 447 344
BmgBI CAC'GTC
Cuts at: 0 1026 2943
Size: 1026 1917
BmrI ACTGGGnnnn_n'
Cuts at: 0 2250 2471 2943
Size: 2250 221 472
Fragments arranged by size:
2250 472 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 802 1332 1436 1595 2788 2932 2943
Size: 464 338 530 104 159 1193 144 11
Fragments arranged by size:
1193 530 464 338 159 144 104 11
Bpu10I CC'TnA_GC
Cuts at: 0 1832 2943
Size: 1832 1111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 183 2943
Size: 100 83 2760
Fragments arranged by size:
2760 100 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 2834 2943
Size: 2834 109
BsaBI GATnn'nnATC
Cuts at: 0 1613 1619 1922 2943
Size: 1613 6 303 1021
Fragments arranged by size:
1613 1021 303 6
BsaHI Gr'CG_yC
Cuts at: 0 150 2943
Size: 150 2793
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 443 473 2685 2715 2943
Size: 443 30 2212 30 228
Fragments arranged by size:
2212 443 228 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 907 1592 2830 2833 2943
Size: 907 685 1238 3 110
Fragments arranged by size:
1238 907 685 110 3
BseYI C'CCAG_C
Cuts at: 0 440 2943
Size: 440 2503
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1934 2943
Size: 1396 538 1009
Fragments arranged by size:
1396 1009 538
BsiEI CG_ry'CG
Cuts at: 0 699 842 2281 2943
Size: 699 143 1439 662
Fragments arranged by size:
1439 699 662 143
BsiHKAI G_wGCw'C
Cuts at: 0 1833 2152 2943
Size: 1833 319 791
Fragments arranged by size:
1833 791 319
BsmI GAATG_Cn'
Cuts at: 0 498 1387 1956 2943
Size: 498 889 569 987
Fragments arranged by size:
987 889 569 498
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2661 2943
Size: 2661 282
Bsp1286I G_dGCh'C
Cuts at: 0 1466 1833 2152 2496 2943
Size: 1466 367 319 344 447
Fragments arranged by size:
1466 447 367 344 319
BspHI T'CATG_A
Cuts at: 0 1521 1611 2082 2943
Size: 1521 90 471 861
Fragments arranged by size:
1521 861 471 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1833 1955 2943
Size: 1833 122 988
Fragments arranged by size:
1833 988 122
BsrBI CCG'CTC
Cuts at: 0 1316 2943
Size: 1316 1627
BsrDI GCAATG_nn'
Cuts at: 0 860 2943
Size: 860 2083
BsrFI r'CCGG_y
Cuts at: 0 13 2943
Size: 13 2930
BssSI C'ACGA_G
Cuts at: 0 86 2943
Size: 86 2857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2766 2910 2943
Size: 2766 144 33
Fragments arranged by size:
2766 144 33
BstYI r'GATC_y
Cuts at: 0 1995 2077 2356 2943
Size: 1995 82 279 587
Fragments arranged by size:
1995 587 279 82
BtsI GCAGTG_nn'
Cuts at: 0 1057 1384 1536 2943
Size: 1057 327 152 1407
Fragments arranged by size:
1407 1057 327 152
ClaI AT'CG_AT
Cuts at: 0 2277 2943
Size: 2277 666
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 240 275 2943
Size: 240 35 2668
Fragments arranged by size:
2668 240 35
EaeI y'GGCC_r
Cuts at: 0 700 1943 2347 2943
Size: 700 1243 404 596
Fragments arranged by size:
1243 700 596 404
EarI CTCTTCn'nnn_
Cuts at: 0 453 2664 2726 2822 2858 2943
Size: 453 2211 62 96 36 85
Fragments arranged by size:
2211 453 96 85 62 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 464 802 1332 1436 1595 2750 2788
Size: 185 279 338 530 104 159 1155 38
Cuts at: 2788 2932 2943
Size: 144 11
Fragments arranged by size:
1155 530 338 279 185 159 144 104
38 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1475 2911 2943
Size: 1475 1436 32
Fragments arranged by size:
1475 1436 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1480 2943
Size: 1480 1463
EcoRV GAT'ATC
Cuts at: 0 2373 2549 2943
Size: 2373 176 394
Fragments arranged by size:
2373 394 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 334 2923 2943
Size: 334 2589 20
Fragments arranged by size:
2589 334 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 1815 1847 2943
Size: 55 32 1728 32 1096
Fragments arranged by size:
1728 1096 55 32 32
HincII GTy'rAC
Cuts at: 0 732 2943
Size: 732 2211
KpnI G_GTAC'C
Cuts at: 0 2063 2943
Size: 2063 880
MfeI C'AATT_G
Cuts at: 0 299 2943
Size: 299 2644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 2943
Size: 862 2081
MscI TGG'CCA
Cuts at: 0 1945 2349 2943
Size: 1945 404 594
Fragments arranged by size:
1945 594 404
MslI CAynn'nnrTG
Cuts at: 0 287 401 647 1139 1775 1928 1939 2943
Size: 287 114 246 492 636 153 11 1004
Fragments arranged by size:
1004 636 492 287 246 153 114 11
MspA1I CmG'CkG
Cuts at: 0 532 2728 2837 2943
Size: 532 2196 109 106
Fragments arranged by size:
2196 532 109 106
NaeI GCC'GGC
Cuts at: 0 15 2943
Size: 15 2928
NdeI CA'TA_TG
Cuts at: 0 792 1166 2943
Size: 792 374 1777
Fragments arranged by size:
1777 792 374
NgoMIV G'CCGG_C
Cuts at: 0 13 2943
Size: 13 2930
NspI r_CATG'y
Cuts at: 0 1283 1829 2943
Size: 1283 546 1114
Fragments arranged by size:
1283 1114 546
PciI A'CATG_T
Cuts at: 0 1825 2943
Size: 1825 1118
PpuMI rG'GwC_Cy
Cuts at: 0 1480 2943
Size: 1480 1463
PspXI vC'TCGA_Gb
Cuts at: 0 221 2943
Size: 221 2722
PstI C_TGCA'G
Cuts at: 0 636 1064 2943
Size: 636 428 1879
Fragments arranged by size:
1879 636 428
PvuI CG_AT'CG
Cuts at: 0 2281 2943
Size: 2281 662
PvuII CAG'CTG
Cuts at: 0 532 2728 2943
Size: 532 2196 215
Fragments arranged by size:
2196 532 215
SapI GCTCTTCn'nnn_
Cuts at: 0 2858 2943
Size: 2858 85
ScaI AGT'ACT
Cuts at: 0 48 878 2943
Size: 48 830 2065
Fragments arranged by size:
2065 830 48
SfcI C'TryA_G
Cuts at: 0 632 813 1060 1457 1670 2072 2624 2943
Size: 632 181 247 397 213 402 552 319
Fragments arranged by size:
632 552 402 397 319 247 213 181
SmlI C'TyrA_G
Cuts at: 0 79 198 221 2943
Size: 79 119 23 2722
Fragments arranged by size:
2722 119 79 23
SspI AAT'ATT
Cuts at: 0 2234 2943
Size: 2234 709
StuI AGG'CCT
Cuts at: 0 1011 2943
Size: 1011 1932
StyI C'CwwG_G
Cuts at: 0 2844 2943
Size: 2844 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 618 1268 2943
Size: 618 650 1675
Fragments arranged by size:
1675 650 618
TatI w'GTAC_w
Cuts at: 0 46 876 1761 2237 2486 2943
Size: 46 830 885 476 249 457
Fragments arranged by size:
885 830 476 457 249 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 443 475 2685 2717 2943
Size: 443 32 2210 32 226
Fragments arranged by size:
2210 443 226 32 32
XhoI C'TCGA_G
Cuts at: 0 221 2943
Size: 221 2722
XmnI GAAnn'nnTTC
Cuts at: 0 1956 2943
Size: 1956 987
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI ApaLI ApoI AseI
AvaI BanI BanII BbsI BciVI BclI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstXI
BstYI BtsI ClaI CspCI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRV FalI Hin4I HincII KpnI MfeI MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NspI PciI
PpuMI PspXI PstI PvuI PvuII SapI ScaI SfcI
SmlI SspI StuI StyI TaqII TatI TstI XhoI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BfrBI BglI BlpI BmtI BplI
BsaAI BsaWI BsiWI BspEI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI FseI FspI FspAI HaeII
HindIII HpaI KasI MluI NarI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XcmI XmaI ZraI