Restriction map for
pF1KE2673
(Linear) (Six-base) MAPSORT of: KIEE2673.seq Check: 6339 from: 1 to: 2379
KIEE2673.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1777 2379
Size: 1777 602
AccI GT'mk_AC
Cuts at: 0 1220 1549 2379
Size: 1220 329 830
Fragments arranged by size:
1220 830 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 380 2379
Size: 121 259 1999
Fragments arranged by size:
1999 259 121
AlwNI CAG_nnn'CTG
Cuts at: 0 990 2379
Size: 990 1389
ApoI r'AATT_y
Cuts at: 0 443 518 757 831 915 1156 1386 1615
Size: 443 75 239 74 84 241 230 229
Cuts at: 1615 2163 2308 2379
Size: 548 145 71
Fragments arranged by size:
548 443 241 239 230 229 145 84
75 74 71
AvaI C'yCGr_G
Cuts at: 0 18 33 1988 2379
Size: 18 15 1955 391
Fragments arranged by size:
1955 391 18 15
BamHI G'GATC_C
Cuts at: 0 693 2379
Size: 693 1686
BanI G'GyrC_C
Cuts at: 0 37 1765 2379
Size: 37 1728 614
Fragments arranged by size:
1728 614 37
BbeI G_GCGC'C
Cuts at: 0 41 2379
Size: 41 2338
BbsI GAAGACnn'nnnn_
Cuts at: 0 1349 1973 2379
Size: 1349 624 406
Fragments arranged by size:
1349 624 406
BfrBI ATG'CAT
Cuts at: 0 1471 2379
Size: 1471 908
BglII A'GATC_T
Cuts at: 0 2298 2379
Size: 2298 81
BlpI GC'TnA_GC
Cuts at: 0 2005 2379
Size: 2005 374
BmrI ACTGGGnnnn_n'
Cuts at: 0 963 2379
Size: 963 1416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 2379
Size: 981 1398
Bpu10I CC'TnA_GC
Cuts at: 0 806 1800 2379
Size: 806 994 579
Fragments arranged by size:
994 806 579
BsaBI GATnn'nnATC
Cuts at: 0 647 2379
Size: 647 1732
BsaHI Gr'CG_yC
Cuts at: 0 38 2379
Size: 38 2341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2019 2049 2379
Size: 2019 30 330
Fragments arranged by size:
2019 330 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 36 39 139 703 2379
Size: 33 3 3 100 564 1676
Fragments arranged by size:
1676 564 100 33 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 25 2379
Size: 25 2354
BsmI GAATG_Cn'
Cuts at: 0 586 624 1471 2379
Size: 586 38 847 908
Fragments arranged by size:
908 847 586 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1540 2379
Size: 1540 839
Bsp1286I G_dGCh'C
Cuts at: 0 25 2379
Size: 25 2354
BspHI T'CATG_A
Cuts at: 0 1382 1440 1962 2379
Size: 1382 58 522 417
Fragments arranged by size:
1382 522 417 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1777 2379
Size: 1777 602
BsrDI GCAATG_nn'
Cuts at: 0 796 1746 1934 2379
Size: 796 950 188 445
Fragments arranged by size:
950 796 445 188
BssSI C'ACGA_G
Cuts at: 0 836 2379
Size: 836 1543
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1408 2379
Size: 1408 971
BstEII G'GTnAC_C
Cuts at: 0 1021 2379
Size: 1021 1358
BstXI CCAn_nnnn'nTGG
Cuts at: 0 571 1920 2379
Size: 571 1349 459
Fragments arranged by size:
1349 571 459
BstYI r'GATC_y
Cuts at: 0 693 2298 2379
Size: 693 1605 81
Fragments arranged by size:
1605 693 81
BstZ17I GTA'TAC
Cuts at: 0 1550 2379
Size: 1550 829
ClaI AT'CG_AT
Cuts at: 0 651 2379
Size: 651 1728
DraI TTT'AAA
Cuts at: 0 731 2150 2379
Size: 731 1419 229
Fragments arranged by size:
1419 731 229
DraIII CAC_nnn'GTG
Cuts at: 0 590 2379
Size: 590 1789
EaeI y'GGCC_r
Cuts at: 0 659 1081 2379
Size: 659 422 1298
Fragments arranged by size:
1298 659 422
EarI CTCTTCn'nnn_
Cuts at: 0 78 2170 2379
Size: 78 2092 209
Fragments arranged by size:
2092 209 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 380 2379
Size: 121 259 1999
Fragments arranged by size:
1999 259 121
EcoRI G'AATT_C
Cuts at: 0 443 757 831 1386 2379
Size: 443 314 74 555 993
Fragments arranged by size:
993 555 443 314 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 168 2379
Size: 168 2211
HaeII r_GCGC'y
Cuts at: 0 41 100 2379
Size: 41 59 2279
Fragments arranged by size:
2279 59 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1944 1976 2094 2126 2379
Size: 1944 32 118 32 253
Fragments arranged by size:
1944 253 118 32 32
HincII GTy'rAC
Cuts at: 0 683 2379
Size: 683 1696
HindIII A'AGCT_T
Cuts at: 0 145 1941 2379
Size: 145 1796 438
Fragments arranged by size:
1796 438 145
HpaI GTT'AAC
Cuts at: 0 683 2379
Size: 683 1696
KasI G'GCGC_C
Cuts at: 0 37 2379
Size: 37 2342
MfeI C'AATT_G
Cuts at: 0 1655 2379
Size: 1655 724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 2379
Size: 1319 1060
MscI TGG'CCA
Cuts at: 0 661 1083 2379
Size: 661 422 1296
Fragments arranged by size:
1296 661 422
NarI GG'CG_CC
Cuts at: 0 38 2379
Size: 38 2341
NsiI A_TGCA'T
Cuts at: 0 1473 2379
Size: 1473 906
NspI r_CATG'y
Cuts at: 0 1810 2379
Size: 1810 569
PshAI GACnn'nnGTC
Cuts at: 0 1014 2379
Size: 1014 1365
PspXI vC'TCGA_Gb
Cuts at: 0 1988 2379
Size: 1988 391
PstI C_TGCA'G
Cuts at: 0 302 333 1853 2379
Size: 302 31 1520 526
Fragments arranged by size:
1520 526 302 31
SfcI C'TryA_G
Cuts at: 0 298 329 422 1849 2379
Size: 298 31 93 1427 530
Fragments arranged by size:
1427 530 298 93 31
SfoI GGC'GCC
Cuts at: 0 39 2379
Size: 39 2340
SmlI C'TyrA_G
Cuts at: 0 1988 2379
Size: 1988 391
TatI w'GTAC_w
Cuts at: 0 2254 2379
Size: 2254 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 822 854 2379
Size: 822 32 1525
Fragments arranged by size:
1525 822 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1563 1759 1920 2379
Size: 1563 196 161 459
Fragments arranged by size:
1563 459 196 161
XhoI C'TCGA_G
Cuts at: 0 1988 2379
Size: 1988 391
XmnI GAAnn'nnTTC
Cuts at: 0 148 443 851 2379
Size: 148 295 408 1528
Fragments arranged by size:
1528 408 295 148
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AvaI BamHI BanI
BbeI BbsI BfrBI BglII BlpI BmrI BplI Bpu10I
BsaBI BsaHI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI
BstZ17I ClaI DraI DraIII EaeI EarI Eco57MI EcoRI
FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MmeI MscI NarI NsiI NspI PshAI PspXI PstI
SfcI SfoI SmlI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbvCI BcgI BciVI BclI BglI
Bme1580I BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaWI
BseYI BsgI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI
BssHII BstBI Bsu36I BtgI BtgZI BtsI CspCI DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI KpnI MluI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XmaI ZraI