Restriction map for
pF1KE2671
(Linear) (Six-base) MAPSORT of: KIEE2671.seq Check: 5217 from: 1 to: 999
KIEE2671.orf
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AleI CACnn'nnGTG
Cuts at: 0 815 999
Size: 815 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 999
Size: 976 23
AlwNI CAG_nnn'CTG
Cuts at: 0 457 999
Size: 457 542
ApaLI G'TGCA_C
Cuts at: 0 826 999
Size: 826 173
AvaI C'yCGr_G
Cuts at: 0 77 188 999
Size: 77 111 811
Fragments arranged by size:
811 111 77
BanI G'GyrC_C
Cuts at: 0 347 377 999
Size: 347 30 622
Fragments arranged by size:
622 347 30
BanII G_rGCy'C
Cuts at: 0 481 999
Size: 481 518
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 999
Size: 89 910
BglI GCCn_nnn'nGGC
Cuts at: 0 37 454 999
Size: 37 417 545
Fragments arranged by size:
545 417 37
BlpI GC'TnA_GC
Cuts at: 0 733 999
Size: 733 266
Bme1580I G_kGCm'C
Cuts at: 0 380 830 999
Size: 380 450 169
Fragments arranged by size:
450 380 169
BmgBI CAC'GTC
Cuts at: 0 474 992 999
Size: 474 518 7
Fragments arranged by size:
518 474 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 409 445 999
Size: 409 36 554
Fragments arranged by size:
554 409 36
BmtI G_CTAG'C
Cuts at: 0 304 999
Size: 304 695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 985 999
Size: 124 861 14
Fragments arranged by size:
861 124 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 886 999
Size: 235 651 113
Fragments arranged by size:
651 235 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 595 999
Size: 595 404
BsaBI GATnn'nnATC
Cuts at: 0 671 999
Size: 671 328
BsaHI Gr'CG_yC
Cuts at: 0 238 854 999
Size: 238 616 145
Fragments arranged by size:
616 238 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 999
Size: 463 30 506
Fragments arranged by size:
506 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 306 547 999
Size: 280 26 241 452
Fragments arranged by size:
452 280 241 26
BsiHKAI G_wGCw'C
Cuts at: 0 830 999
Size: 830 169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 502 999
Size: 502 497
Bsp1286I G_dGCh'C
Cuts at: 0 380 481 830 999
Size: 380 101 349 169
Fragments arranged by size:
380 349 169 101
BsrDI GCAATG_nn'
Cuts at: 0 587 830 999
Size: 587 243 169
Fragments arranged by size:
587 243 169
BsrFI r'CCGG_y
Cuts at: 0 380 835 999
Size: 380 455 164
Fragments arranged by size:
455 380 164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 999
Size: 384 615
BstXI CCAn_nnnn'nTGG
Cuts at: 0 801 999
Size: 801 198
BstYI r'GATC_y
Cuts at: 0 282 999
Size: 282 717
Bsu36I CC'TnA_GG
Cuts at: 0 343 999
Size: 343 656
BtgI C'CryG_G
Cuts at: 0 754 779 908 999
Size: 754 25 129 91
Fragments arranged by size:
754 129 91 25
EaeI y'GGCC_r
Cuts at: 0 29 791 947 999
Size: 29 762 156 52
Fragments arranged by size:
762 156 52 29
EarI CTCTTCn'nnn_
Cuts at: 0 505 999
Size: 505 494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 985 999
Size: 124 861 14
Fragments arranged by size:
861 124 14
EcoO109I rG'GnC_Cy
Cuts at: 0 942 999
Size: 942 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 395 427 463 495 999
Size: 267 32 96 32 36 32 504
Fragments arranged by size:
504 267 96 36 32 32 32
MscI TGG'CCA
Cuts at: 0 31 793 949 999
Size: 31 762 156 50
Fragments arranged by size:
762 156 50 31
MslI CAynn'nnrTG
Cuts at: 0 815 884 999
Size: 815 69 115
Fragments arranged by size:
815 115 69
MspA1I CmG'CkG
Cuts at: 0 17 356 721 999
Size: 17 339 365 278
Fragments arranged by size:
365 339 278 17
NaeI GCC'GGC
Cuts at: 0 837 999
Size: 837 162
NcoI C'CATG_G
Cuts at: 0 908 999
Size: 908 91
NgoMIV G'CCGG_C
Cuts at: 0 835 999
Size: 835 164
NheI G'CTAG_C
Cuts at: 0 300 999
Size: 300 699
PflMI CCAn_nnn'nTGG
Cuts at: 0 457 999
Size: 457 542
PvuII CAG'CTG
Cuts at: 0 721 999
Size: 721 278
SmaI CCC'GGG
Cuts at: 0 79 190 999
Size: 79 111 809
Fragments arranged by size:
809 111 79
SmlI C'TyrA_G
Cuts at: 0 214 901 999
Size: 214 687 98
Fragments arranged by size:
687 214 98
StuI AGG'CCT
Cuts at: 0 163 999
Size: 163 836
StyI C'CwwG_G
Cuts at: 0 269 908 999
Size: 269 639 91
Fragments arranged by size:
639 269 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 545 577 999
Size: 545 32 422
Fragments arranged by size:
545 422 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 786 999
Size: 39 747 213
Fragments arranged by size:
747 213 39
XmaI C'CCGG_G
Cuts at: 0 77 188 999
Size: 77 111 811
Fragments arranged by size:
811 111 77
Enzymes that do cut:
AleI AloI AlwNI ApaLI AvaI BanI BanII BbsI
BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI EaeI
EarI Eco57MI EcoO109I Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NheI PflMI PvuII SmaI SmlI StuI
StyI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BplI Bpu10I BsaAI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XhoI XmnI ZraI