(Linear) (Six-base) MAPSORT of: KIEE2666.seq  Check: 6422  from: 1  to: 765



KIEE2666.orf

 With 182 enzymes: SgfI * 

                        January 12, 2017 11:32  ..

AclI AA'CG_TT

Cuts at:      0     269     765 
   Size:        269     496
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     765 
   Size:        356     409
 
AflIII A'CryG_T

Cuts at:      0     686     765 
   Size:        686      79
 
ApoI r'AATT_y

Cuts at:      0     176     558     765 
   Size:        176     382     207

  Fragments arranged by size: 

                 382     207     176
 
AseI AT'TA_AT

Cuts at:      0     482     765 
   Size:        482     283
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     603     765 
   Size:        603     162
 
BglII A'GATC_T

Cuts at:      0      12     324     765 
   Size:         12     312     441

  Fragments arranged by size: 

                 441     312      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     272     765 
   Size:        193      79     493

  Fragments arranged by size: 

                 493     193      79
 
BsaAI yAC'GTr

Cuts at:      0     687     765 
   Size:        687      78
 
BsaBI GATnn'nnATC

Cuts at:      0     329     765 
   Size:        329     436
 
BstYI r'GATC_y

Cuts at:      0      12     324     765 
   Size:         12     312     441

  Fragments arranged by size: 

                 441     312      12
 
BtsI GCAGTG_nn'

Cuts at:      0     708     765 
   Size:        708      57
 
EaeI y'GGCC_r

Cuts at:      0      87     765 
   Size:         87     678
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     765 
   Size:        356     409
 
EcoRI G'AATT_C

Cuts at:      0     558     765 
   Size:        558     207
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      84     765 
   Size:         84     681
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     119     151     558     590     765 
   Size:        119      32     407      32     175

  Fragments arranged by size: 

                 407     175     119      32      32
 
MscI TGG'CCA

Cuts at:      0      89     765 
   Size:         89     676
 
MslI CAynn'nnrTG

Cuts at:      0      38     765 
   Size:         38     727
 
PmlI CAC'GTG

Cuts at:      0     687     765 
   Size:        687      78
 
PsiI TTA'TAA

Cuts at:      0     418     760     765 
   Size:        418     342       5

  Fragments arranged by size: 

                 418     342       5
 
SmlI C'TyrA_G

Cuts at:      0     208     287     765 
   Size:        208      79     478

  Fragments arranged by size: 

                 478     208      79
 
SspI AAT'ATT

Cuts at:      0      57     699     765 
   Size:         57     642      66

  Fragments arranged by size: 

                 642      66      57
 
XmnI GAAnn'nnTTC

Cuts at:      0     269     765 
   Size:        269     496
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     ApoI     AseI    BciVI    BglII    BpuEI
    BsaAI    BsaBI    BstYI     BtsI     EaeI  Eco57MI    EcoRI     FalI
    Hin4I     MscI     MslI     PmlI     PsiI     SmlI     SspI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI