(Linear) (Six-base) MAPSORT of: KIEE2664.seq  Check: 4979  from: 1  to: 432



KIEE2664.orf

 With 182 enzymes: SgfI * 

                        January 12, 2017 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     432 
   Size:         85     347
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57     432 
   Size:         57     375
 
BanII G_rGCy'C

Cuts at:      0      44     432 
   Size:         44     388
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     161     432 
   Size:        161     271
 
Bme1580I G_kGCm'C

Cuts at:      0     103     162     376     432 
   Size:        103      59     214      56

  Fragments arranged by size: 

                 214     103      59      56
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     379     432 
   Size:        379      53
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     432 
   Size:        338      94
 
Bpu10I CC'TnA_GC

Cuts at:      0      58     432 
   Size:         58     374
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     408     432 
   Size:        408      24
 
BsaBI GATnn'nnATC

Cuts at:      0     268     432 
   Size:        268     164
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     432 
   Size:        326     106
 
BsiEI CG_ry'CG

Cuts at:      0      38     432 
   Size:         38     394
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     103     162     376     432 
   Size:         44      59      59     214      56

  Fragments arranged by size: 

                 214      59      59      56      44
 
BsrBI CCG'CTC

Cuts at:      0      17     432 
   Size:         17     415
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     313     432 
   Size:        313     119
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     137     432 
   Size:        137     295
 
BstYI r'GATC_y

Cuts at:      0      53     432 
   Size:         53     379
 
BtgI C'CryG_G

Cuts at:      0      76     432 
   Size:         76     356
 
EaeI y'GGCC_r

Cuts at:      0      35     432 
   Size:         35     397
 
EagI C'GGCC_G

Cuts at:      0      35     432 
   Size:         35     397
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     214     432 
   Size:        181      33     218

  Fragments arranged by size: 

                 218     181      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     338     432 
   Size:         85     253      94

  Fragments arranged by size: 

                 253      94      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0      92     432 
   Size:         92     340
 
MslI CAynn'nnrTG

Cuts at:      0     230     432 
   Size:        230     202
 
MspA1I CmG'CkG

Cuts at:      0      26      78     432 
   Size:         26      52     354

  Fragments arranged by size: 

                 354      52      26
 
PpuMI rG'GwC_Cy

Cuts at:      0      92     432 
   Size:         92     340
 
PstI C_TGCA'G

Cuts at:      0      50     432 
   Size:         50     382
 
SacII CC_GC'GG

Cuts at:      0      79     432 
   Size:         79     353
 
SanDI GG'GwC_CC

Cuts at:      0      92     432 
   Size:         92     340
 
SfcI C'TryA_G

Cuts at:      0      46     432 
   Size:         46     386
 

 Enzymes that do cut:

     AcuI    AlwNI    BanII     BbsI Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaBI     BsgI    BsiEI Bsp1286I    BsrBI   BstAPI    BstXI
    BstYI     BtgI     EaeI     EagI     EarI  Eco57MI EcoO109I     MslI
   MspA1I    PpuMI     PstI    SacII    SanDI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI