Restriction map for
pF1KE2662
(Linear) (Six-base) MAPSORT of: KIEE2662.seq Check: 1213 from: 1 to: 741
KIEE2662.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 235 340 741
Size: 235 105 401
Fragments arranged by size:
401 235 105
AccI GT'mk_AC
Cuts at: 0 371 741
Size: 371 370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 741
Size: 234 507
AflIII A'CryG_T
Cuts at: 0 470 741
Size: 470 271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 426 741
Size: 426 315
AvaI C'yCGr_G
Cuts at: 0 679 741
Size: 679 62
BanII G_rGCy'C
Cuts at: 0 224 238 626 655 741
Size: 224 14 388 29 86
Fragments arranged by size:
388 224 86 29 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 111 741
Size: 111 630
BglI GCCn_nnn'nGGC
Cuts at: 0 277 741
Size: 277 464
BglII A'GATC_T
Cuts at: 0 377 741
Size: 377 364
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 741
Size: 144 597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 741
Size: 610 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 741
Size: 451 290
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 634 741
Size: 634 107
BseYI C'CCAG_C
Cuts at: 0 446 521 588 741
Size: 446 75 67 153
Fragments arranged by size:
446 153 75 67
BsiEI CG_ry'CG
Cuts at: 0 284 741
Size: 284 457
BsiHKAI G_wGCw'C
Cuts at: 0 41 157 224 626 741
Size: 41 116 67 402 115
Fragments arranged by size:
402 116 115 67 41
Bsp1286I G_dGCh'C
Cuts at: 0 41 157 224 238 626 655 741
Size: 41 116 67 14 388 29 86
Fragments arranged by size:
388 116 86 67 41 29 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 235 340 617 741
Size: 235 105 277 124
Fragments arranged by size:
277 235 124 105
BsrFI r'CCGG_y
Cuts at: 0 596 741
Size: 596 145
BssSI C'ACGA_G
Cuts at: 0 34 741
Size: 34 707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 332 741
Size: 45 287 409
Fragments arranged by size:
409 287 45
BstYI r'GATC_y
Cuts at: 0 377 741
Size: 377 364
BtgI C'CryG_G
Cuts at: 0 305 719 741
Size: 305 414 22
Fragments arranged by size:
414 305 22
EaeI y'GGCC_r
Cuts at: 0 281 536 741
Size: 281 255 205
Fragments arranged by size:
281 255 205
EagI C'GGCC_G
Cuts at: 0 281 741
Size: 281 460
EarI CTCTTCn'nnn_
Cuts at: 0 187 741
Size: 187 554
EcoICRI GAG'CTC
Cuts at: 0 222 624 741
Size: 222 402 117
Fragments arranged by size:
402 222 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 741
Size: 234 507
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 741
Size: 613 128
EcoO109I rG'GnC_Cy
Cuts at: 0 736 741
Size: 736 5
HaeII r_GCGC'y
Cuts at: 0 272 741
Size: 272 469
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 741
Size: 689 32 20
Fragments arranged by size:
689 32 20
MscI TGG'CCA
Cuts at: 0 538 741
Size: 538 203
MslI CAynn'nnrTG
Cuts at: 0 67 109 741
Size: 67 42 632
Fragments arranged by size:
632 67 42
MspA1I CmG'CkG
Cuts at: 0 203 741
Size: 203 538
NcoI C'CATG_G
Cuts at: 0 305 741
Size: 305 436
NotI GC'GGCC_GC
Cuts at: 0 281 741
Size: 281 460
NspI r_CATG'y
Cuts at: 0 48 474 741
Size: 48 426 267
Fragments arranged by size:
426 267 48
PasI CC'CwG_GG
Cuts at: 0 501 741
Size: 501 240
PciI A'CATG_T
Cuts at: 0 470 741
Size: 470 271
PfoI T'CCnGG_A
Cuts at: 0 464 741
Size: 464 277
PpuMI rG'GwC_Cy
Cuts at: 0 736 741
Size: 736 5
PstI C_TGCA'G
Cuts at: 0 617 741
Size: 617 124
SacI G_AGCT'C
Cuts at: 0 224 626 741
Size: 224 402 115
Fragments arranged by size:
402 224 115
SanDI GG'GwC_CC
Cuts at: 0 736 741
Size: 736 5
ScaI AGT'ACT
Cuts at: 0 496 741
Size: 496 245
SfcI C'TryA_G
Cuts at: 0 613 656 741
Size: 613 43 85
Fragments arranged by size:
613 85 43
SmaI CCC'GGG
Cuts at: 0 681 741
Size: 681 60
SmlI C'TyrA_G
Cuts at: 0 625 741
Size: 625 116
StuI AGG'CCT
Cuts at: 0 19 741
Size: 19 722
StyI C'CwwG_G
Cuts at: 0 305 741
Size: 305 436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 126 741
Size: 126 615
TatI w'GTAC_w
Cuts at: 0 494 741
Size: 494 247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 741
Size: 108 633
XmaI C'CCGG_G
Cuts at: 0 679 741
Size: 679 62
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AvaI BanII BbsI
BglI BglII BmrI BpuEI BsaI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstYI BtgI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MscI MslI MspA1I NcoI NotI NspI PasI
PciI PfoI PpuMI PstI SacI SanDI ScaI SfcI
SmaI SmlI StuI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI