Restriction map for
pF1KE2661
(Linear) (Six-base) MAPSORT of: KIEE2661.seq Check: 3435 from: 1 to: 1935
KIEE2661.orf
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AccI GT'mk_AC
Cuts at: 0 683 1935
Size: 683 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 1516 1560 1935
Size: 1444 72 44 375
Fragments arranged by size:
1444 375 72 44
AlwNI CAG_nnn'CTG
Cuts at: 0 1226 1935
Size: 1226 709
ApoI r'AATT_y
Cuts at: 0 828 901 1096 1935
Size: 828 73 195 839
Fragments arranged by size:
839 828 195 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 1052 1935
Size: 69 983 883
Fragments arranged by size:
983 883 69
BfrBI ATG'CAT
Cuts at: 0 793 1739 1935
Size: 793 946 196
Fragments arranged by size:
946 793 196
BglI GCCn_nnn'nGGC
Cuts at: 0 1886 1935
Size: 1886 49
BmgBI CAC'GTC
Cuts at: 0 681 1935
Size: 681 1254
BmrI ACTGGGnnnn_n'
Cuts at: 0 1920 1935
Size: 1920 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1524 1800 1935
Size: 1524 276 135
Fragments arranged by size:
1524 276 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1935
Size: 176 1759
Bpu10I CC'TnA_GC
Cuts at: 0 1341 1935
Size: 1341 594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1682 1882 1924 1935
Size: 1682 200 42 11
Fragments arranged by size:
1682 200 42 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 1865 1935
Size: 1865 70
BsaBI GATnn'nnATC
Cuts at: 0 38 1490 1935
Size: 38 1452 445
Fragments arranged by size:
1452 445 38
BsaHI Gr'CG_yC
Cuts at: 0 1843 1935
Size: 1843 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 152 182 704 734 1935
Size: 25 127 30 522 30 1201
Fragments arranged by size:
1201 522 127 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 173 1337 1390 1445 1500 1776 1935
Size: 173 1164 53 55 55 276 159
Fragments arranged by size:
1164 276 173 159 55 55 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1935
Size: 661 1274
BsiHKAI G_wGCw'C
Cuts at: 0 1569 1935
Size: 1569 366
BsmI GAATG_Cn'
Cuts at: 0 808 1741 1935
Size: 808 933 194
Fragments arranged by size:
933 808 194
Bsp1286I G_dGCh'C
Cuts at: 0 1569 1935
Size: 1569 366
BsrBI CCG'CTC
Cuts at: 0 61 1935
Size: 61 1874
BsrDI GCAATG_nn'
Cuts at: 0 170 866 1935
Size: 170 696 1069
Fragments arranged by size:
1069 696 170
BsrFI r'CCGG_y
Cuts at: 0 937 988 1935
Size: 937 51 947
Fragments arranged by size:
947 937 51
BsrGI T'GTAC_A
Cuts at: 0 695 1935
Size: 695 1240
BssSI C'ACGA_G
Cuts at: 0 1402 1935
Size: 1402 533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 1564 1935
Size: 799 765 371
Fragments arranged by size:
799 765 371
BstYI r'GATC_y
Cuts at: 0 478 642 1277 1935
Size: 478 164 635 658
Fragments arranged by size:
658 635 478 164
BtsI GCAGTG_nn'
Cuts at: 0 740 1935
Size: 740 1195
ClaI AT'CG_AT
Cuts at: 0 132 1935
Size: 132 1803
DraI TTT'AAA
Cuts at: 0 123 1935
Size: 123 1812
EaeI y'GGCC_r
Cuts at: 0 573 659 737 1935
Size: 573 86 78 1198
Fragments arranged by size:
1198 573 86 78
EarI CTCTTCn'nnn_
Cuts at: 0 448 1021 1579 1935
Size: 448 573 558 356
Fragments arranged by size:
573 558 448 356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1129 1935
Size: 1129 806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1444 1516 1560 1935
Size: 176 1268 72 44 375
Fragments arranged by size:
1268 375 176 72 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 353 1935
Size: 353 1582
EcoO109I rG'GnC_Cy
Cuts at: 0 1503 1935
Size: 1503 432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 1935
Size: 477 1458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 372 404 1313 1345 1495 1527 1935
Size: 27 345 32 909 32 150 32 408
Fragments arranged by size:
909 408 345 150 32 32 32 27
HincII GTy'rAC
Cuts at: 0 684 896 1451 1935
Size: 684 212 555 484
Fragments arranged by size:
684 555 484 212
HindIII A'AGCT_T
Cuts at: 0 1829 1900 1935
Size: 1829 71 35
Fragments arranged by size:
1829 71 35
MscI TGG'CCA
Cuts at: 0 575 661 739 1935
Size: 575 86 78 1196
Fragments arranged by size:
1196 575 86 78
MslI CAynn'nnrTG
Cuts at: 0 1303 1935
Size: 1303 632
MspA1I CmG'CkG
Cuts at: 0 579 1641 1935
Size: 579 1062 294
Fragments arranged by size:
1062 579 294
NaeI GCC'GGC
Cuts at: 0 990 1935
Size: 990 945
NgoMIV G'CCGG_C
Cuts at: 0 988 1935
Size: 988 947
NsiI A_TGCA'T
Cuts at: 0 795 1741 1935
Size: 795 946 194
Fragments arranged by size:
946 795 194
NspI r_CATG'y
Cuts at: 0 571 1935
Size: 571 1364
PflMI CCAn_nnn'nTGG
Cuts at: 0 822 1935
Size: 822 1113
PpuMI rG'GwC_Cy
Cuts at: 0 1503 1935
Size: 1503 432
PsiI TTA'TAA
Cuts at: 0 181 1935
Size: 181 1754
PstI C_TGCA'G
Cuts at: 0 1798 1935
Size: 1798 137
PvuII CAG'CTG
Cuts at: 0 579 1935
Size: 579 1356
SalI G'TCGA_C
Cuts at: 0 682 1935
Size: 682 1253
SapI GCTCTTCn'nnn_
Cuts at: 0 1579 1935
Size: 1579 356
SfcI C'TryA_G
Cuts at: 0 206 638 1794 1935
Size: 206 432 1156 141
Fragments arranged by size:
1156 432 206 141
SmlI C'TyrA_G
Cuts at: 0 1697 1897 1903 1935
Size: 1697 200 6 32
Fragments arranged by size:
1697 200 32 6
StyI C'CwwG_G
Cuts at: 0 776 1935
Size: 776 1159
TatI w'GTAC_w
Cuts at: 0 695 1935
Size: 695 1240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 152 184 704 736 1935
Size: 152 32 520 32 1199
Fragments arranged by size:
1199 520 152 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 583 1935
Size: 583 1352
Enzymes that do cut:
AccI AcuI AlwNI ApoI BbsI BfrBI BglI BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI BtsI ClaI DraI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII HindIII MscI MslI MspA1I NaeI NgoMIV NsiI
NspI PflMI PpuMI PsiI PstI PvuII SalI SapI
SfcI SmlI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmtI
BsaAI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI CspCI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI NarI NcoI NdeI NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI ZraI