Restriction map for
pF1KE2658
(Linear) (Six-base) MAPSORT of: KIEE2658.seq Check: 1990 from: 1 to: 3396
KIEE2658.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2767 3167 3396
Size: 2767 400 229
Fragments arranged by size:
2767 400 229
AatII G_ACGT'C
Cuts at: 0 3387 3396
Size: 3387 9
Acc65I G'GTAC_C
Cuts at: 0 2080 3396
Size: 2080 1316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 772 1525 1568 1832 2036 2654 3396
Size: 140 632 753 43 264 204 618 742
Fragments arranged by size:
753 742 632 618 264 204 140 43
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2532 3396
Size: 2532 864
AlwNI CAG_nnn'CTG
Cuts at: 0 921 1099 1685 2283 3396
Size: 921 178 586 598 1113
Fragments arranged by size:
1113 921 598 586 178
ApaI G_GGCC'C
Cuts at: 0 232 962 1029 3396
Size: 232 730 67 2367
Fragments arranged by size:
2367 730 232 67
ApoI r'AATT_y
Cuts at: 0 614 3396
Size: 614 2782
AvaI C'yCGr_G
Cuts at: 0 354 3396
Size: 354 3042
AvrII C'CTAG_G
Cuts at: 0 597 3396
Size: 597 2799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2064 2097 2312 2345 3396
Size: 2064 33 215 33 1051
Fragments arranged by size:
2064 1051 215 33 33
BamHI G'GATC_C
Cuts at: 0 2808 3396
Size: 2808 588
BanI G'GyrC_C
Cuts at: 0 677 1074 1443 1555 2080 3396
Size: 677 397 369 112 525 1316
Fragments arranged by size:
1316 677 525 397 369 112
BanII G_rGCy'C
Cuts at: 0 232 302 962 1029 2290 3228 3276 3396
Size: 232 70 660 67 1261 938 48 120
Fragments arranged by size:
1261 938 660 232 120 70 67 48
BbeI G_GCGC'C
Cuts at: 0 1447 1559 3396
Size: 1447 112 1837
Fragments arranged by size:
1837 1447 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 424 2549 3396
Size: 424 2125 847
Fragments arranged by size:
2125 847 424
BbvCI CC'TCA_GC
Cuts at: 0 479 872 3396
Size: 479 393 2524
Fragments arranged by size:
2524 479 393
BciVI GTATCCnnnnn_n'
Cuts at: 0 1223 2331 3396
Size: 1223 1108 1065
Fragments arranged by size:
1223 1108 1065
BclI T'GATC_A
Cuts at: 0 2103 3396
Size: 2103 1293
BglI GCCn_nnn'nGGC
Cuts at: 0 1821 3396
Size: 1821 1575
BglII A'GATC_T
Cuts at: 0 1311 3396
Size: 1311 2085
BlpI GC'TnA_GC
Cuts at: 0 296 2170 3396
Size: 296 1874 1226
Fragments arranged by size:
1874 1226 296
Bme1580I G_kGCm'C
Cuts at: 0 232 361 521 962 1029 1826 2039 3396
Size: 232 129 160 441 67 797 213 1357
Fragments arranged by size:
1357 797 441 232 213 160 129 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 721 1163 1895 2293 2489 2560 2882 3396
Size: 721 442 732 398 196 71 322 514
Fragments arranged by size:
732 721 514 442 398 322 196 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2637 3066 3396
Size: 2637 429 330
Fragments arranged by size:
2637 429 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 449 790 2274 2336 3191 3254 3396
Size: 152 297 341 1484 62 855 63 142
Fragments arranged by size:
1484 855 341 297 152 142 63 62
Bpu10I CC'TnA_GC
Cuts at: 0 479 872 1231 3396
Size: 479 393 359 2165
Fragments arranged by size:
2165 479 393 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1461 1997 2466 3396
Size: 397 1064 536 469 930
Fragments arranged by size:
1064 930 536 469 397
BsaI GGTCTCn'nnnn_
Cuts at: 0 917 1050 1231 3396
Size: 917 133 181 2165
Fragments arranged by size:
2165 917 181 133
BsaBI GATnn'nnATC
Cuts at: 0 1796 2108 2813 3396
Size: 1796 312 705 583
Fragments arranged by size:
1796 705 583 312
BsaHI Gr'CG_yC
Cuts at: 0 1038 1444 1556 3384 3396
Size: 1038 406 112 1828 12
Fragments arranged by size:
1828 1038 406 112 12
BsaWI w'CCGG_w
Cuts at: 0 33 824 3396
Size: 33 791 2572
Fragments arranged by size:
2572 791 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 557 587 2357 2387 2862 2892 3396
Size: 557 30 1770 30 475 30 504
Fragments arranged by size:
1770 557 504 475 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 787 889 1213 1372 1441 1761 1922 2233
Size: 787 102 324 159 69 320 161 311
Cuts at: 2233 2613 2666 3396
Size: 380 53 730
Fragments arranged by size:
787 730 380 324 320 311 161 159
102 69 53
BseYI C'CCAG_C
Cuts at: 0 199 247 1391 1549 1678 1685 1810 2128
Size: 199 48 1144 158 129 7 125 318
Cuts at: 2128 3184 3396
Size: 1056 212
Fragments arranged by size:
1144 1056 318 212 199 158 129 125
48 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 371 814 3396
Size: 62 309 443 2582
Fragments arranged by size:
2582 443 309 62
BsiHKAI G_wGCw'C
Cuts at: 0 809 1897 3228 3276 3396
Size: 809 1088 1331 48 120
Fragments arranged by size:
1331 1088 809 120 48
BsmI GAATG_Cn'
Cuts at: 0 2946 3396
Size: 2946 450
Bsp1286I G_dGCh'C
Cuts at: 0 232 302 361 521 809 962 1029 1826
Size: 232 70 59 160 288 153 67 797
Cuts at: 1826 1897 2039 2290 3228 3276 3396
Size: 71 142 251 938 48 120
Fragments arranged by size:
938 797 288 251 232 160 153 142
120 71 70 67 59 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1700 2767 3167 3396
Size: 1700 1067 400 229
Fragments arranged by size:
1700 1067 400 229
BsrBI CCG'CTC
Cuts at: 0 608 3396
Size: 608 2788
BsrDI GCAATG_nn'
Cuts at: 0 22 2524 3396
Size: 22 2502 872
Fragments arranged by size:
2502 872 22
BsrFI r'CCGG_y
Cuts at: 0 1034 3396
Size: 1034 2362
BsrGI T'GTAC_A
Cuts at: 0 992 2208 3396
Size: 992 1216 1188
Fragments arranged by size:
1216 1188 992
BstEII G'GTnAC_C
Cuts at: 0 601 3396
Size: 601 2795
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2165 2908 3396
Size: 2165 743 488
Fragments arranged by size:
2165 743 488
BstYI r'GATC_y
Cuts at: 0 1085 1218 1311 1338 2808 3396
Size: 1085 133 93 27 1470 588
Fragments arranged by size:
1470 1085 588 133 93 27
Bsu36I CC'TnA_GG
Cuts at: 0 1070 3126 3396
Size: 1070 2056 270
Fragments arranged by size:
2056 1070 270
BtgI C'CryG_G
Cuts at: 0 2087 3396
Size: 2087 1309
BtsI GCAGTG_nn'
Cuts at: 0 1824 2039 2181 2355 3396
Size: 1824 215 142 174 1041
Fragments arranged by size:
1824 1041 215 174 142
DrdI GACnn_nn'nnGTC
Cuts at: 0 3358 3396
Size: 3358 38
EaeI y'GGCC_r
Cuts at: 0 535 575 1148 1794 3396
Size: 535 40 573 646 1602
Fragments arranged by size:
1602 646 573 535 40
EarI CTCTTCn'nnn_
Cuts at: 0 1418 2055 3029 3396
Size: 1418 637 974 367
Fragments arranged by size:
1418 974 637 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 464 3396
Size: 464 2932
EcoICRI GAG'CTC
Cuts at: 0 3226 3274 3396
Size: 3226 48 122
Fragments arranged by size:
3226 122 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 152 449 772 790 1525 1568 1832
Size: 140 12 297 323 18 735 43 264
Cuts at: 1832 2036 2274 2336 2654 3191 3254 3396
Size: 204 238 62 318 537 63 142
Fragments arranged by size:
735 537 323 318 297 264 238 204
142 140 63 62 43 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 596 772 1570 1937 3396
Size: 596 176 798 367 1459
Fragments arranged by size:
1459 798 596 367 176
EcoO109I rG'GnC_Cy
Cuts at: 0 228 229 382 460 568 918 958 959
Size: 228 1 153 78 108 350 40 1
Cuts at: 959 1026 1689 1729 2311 2419 2904 3181 3285
Size: 67 663 40 582 108 485 277 104
Cuts at: 3285 3396
Size: 111
Fragments arranged by size:
663 582 485 350 277 228 153 111
108 108 104 78 67 40 40 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2587 3396
Size: 2587 809
HaeII r_GCGC'y
Cuts at: 0 1447 1466 1559 1811 3396
Size: 1447 19 93 252 1585
Fragments arranged by size:
1585 1447 252 93 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 1303 1335 2088 2120 2608 2640
Size: 1 32 1270 32 753 32 488 32
Cuts at: 2640 3396
Size: 756
Fragments arranged by size:
1270 756 753 488 32 32 32 32
1
HincII GTy'rAC
Cuts at: 0 1116 2703 3396
Size: 1116 1587 693
Fragments arranged by size:
1587 1116 693
HindIII A'AGCT_T
Cuts at: 0 530 3396
Size: 530 2866
KasI G'GCGC_C
Cuts at: 0 1443 1555 3396
Size: 1443 112 1841
Fragments arranged by size:
1841 1443 112
KpnI G_GTAC'C
Cuts at: 0 2084 3396
Size: 2084 1312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1451 2663 2760 3077 3396
Size: 1451 1212 97 317 319
Fragments arranged by size:
1451 1212 319 317 97
MscI TGG'CCA
Cuts at: 0 537 577 1150 1796 3396
Size: 537 40 573 646 1600
Fragments arranged by size:
1600 646 573 537 40
MslI CAynn'nnrTG
Cuts at: 0 1655 1790 2495 2580 2780 3396
Size: 1655 135 705 85 200 616
Fragments arranged by size:
1655 705 616 200 135 85
MspA1I CmG'CkG
Cuts at: 0 295 900 1395 1685 2128 2383 3396
Size: 295 605 495 290 443 255 1013
Fragments arranged by size:
1013 605 495 443 295 290 255
NaeI GCC'GGC
Cuts at: 0 1036 3396
Size: 1036 2360
NarI GG'CG_CC
Cuts at: 0 1444 1556 3396
Size: 1444 112 1840
Fragments arranged by size:
1840 1444 112
NcoI C'CATG_G
Cuts at: 0 2087 3396
Size: 2087 1309
NgoMIV G'CCGG_C
Cuts at: 0 1034 3396
Size: 1034 2362
NspI r_CATG'y
Cuts at: 0 2585 3396
Size: 2585 811
PasI CC'CwG_GG
Cuts at: 0 125 2039 2307 3290 3396
Size: 125 1914 268 983 106
Fragments arranged by size:
1914 983 268 125 106
PflMI CCAn_nnn'nTGG
Cuts at: 0 1685 2283 3396
Size: 1685 598 1113
Fragments arranged by size:
1685 1113 598
PfoI T'CCnGG_A
Cuts at: 0 1725 2313 3396
Size: 1725 588 1083
Fragments arranged by size:
1725 1083 588
PpuMI rG'GwC_Cy
Cuts at: 0 382 460 568 1689 1729 2311 2419 3285
Size: 382 78 108 1121 40 582 108 866
Cuts at: 3285 3396
Size: 111
Fragments arranged by size:
1121 866 582 382 111 108 108 78
40
PshAI GACnn'nnGTC
Cuts at: 0 1478 3396
Size: 1478 1918
PsiI TTA'TAA
Cuts at: 0 1768 3396
Size: 1768 1628
PspOMI G'GGCC_C
Cuts at: 0 228 958 1025 3396
Size: 228 730 67 2371
Fragments arranged by size:
2371 730 228 67
PstI C_TGCA'G
Cuts at: 0 1697 3396
Size: 1697 1699
PvuII CAG'CTG
Cuts at: 0 295 900 1395 1685 2128 2383 3396
Size: 295 605 495 290 443 255 1013
Fragments arranged by size:
1013 605 495 443 295 290 255
SacI G_AGCT'C
Cuts at: 0 3228 3276 3396
Size: 3228 48 120
Fragments arranged by size:
3228 120 48
SanDI GG'GwC_CC
Cuts at: 0 1729 3285 3396
Size: 1729 1556 111
Fragments arranged by size:
1729 1556 111
SapI GCTCTTCn'nnn_
Cuts at: 0 2055 3396
Size: 2055 1341
SexAI A'CCwGG_T
Cuts at: 0 1772 3396
Size: 1772 1624
SfcI C'TryA_G
Cuts at: 0 1693 2189 3396
Size: 1693 496 1207
Fragments arranged by size:
1693 1207 496
SfoI GGC'GCC
Cuts at: 0 1445 1557 3396
Size: 1445 112 1839
Fragments arranged by size:
1839 1445 112
SgrAI Cr'CCGG_yG
Cuts at: 0 1034 3396
Size: 1034 2362
SmaI CCC'GGG
Cuts at: 0 356 3396
Size: 356 3040
SmlI C'TyrA_G
Cuts at: 0 376 1476 2012 2445 3396
Size: 376 1100 536 433 951
Fragments arranged by size:
1100 951 536 433 376
StuI AGG'CCT
Cuts at: 0 374 470 3396
Size: 374 96 2926
Fragments arranged by size:
2926 374 96
StyI C'CwwG_G
Cuts at: 0 597 620 665 1079 1274 1343 1418 1566
Size: 597 23 45 414 195 69 75 148
Cuts at: 1566 1733 2087 2586 2790 2907 3094 3396
Size: 167 354 499 204 117 187 302
Fragments arranged by size:
597 499 414 354 302 204 195 187
167 148 117 75 69 45 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 138 1092 1726 2592 3396
Size: 134 4 954 634 866 804
Fragments arranged by size:
954 866 804 634 134 4
TatI w'GTAC_w
Cuts at: 0 992 2208 3396
Size: 992 1216 1188
Fragments arranged by size:
1216 1188 992
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 557 589 3396
Size: 557 32 2807
Fragments arranged by size:
2807 557 32
Tth111I GACn'n_nGTC
Cuts at: 0 2143 3396
Size: 2143 1253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 519 765 3396
Size: 207 312 246 2631
Fragments arranged by size:
2631 312 246 207
XmaI C'CCGG_G
Cuts at: 0 354 3396
Size: 354 3042
XmnI GAAnn'nnTTC
Cuts at: 0 1575 2570 3396
Size: 1575 995 826
Fragments arranged by size:
1575 995 826
ZraI GAC'GTC
Cuts at: 0 3385 3396
Size: 3385 11
Enzymes that do cut:
AarI AatII Acc65I AcuI AlfI AlwNI ApaI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI BcgI BfrBI BmgBI
BmtI BsaAI BsiEI BsiWI BsmBI BspEI BspHI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII EagI EcoRI EcoRV FseI FspI FspAI HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PspXI PsrI PvuI RsrII
SacII SalI SbfI ScaI SfiI SgfI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI