Restriction map for
pF1KE2657
(Linear) (Six-base) MAPSORT of: KIEE2657.seq Check: 4733 from: 1 to: 591
KIEE2657.orf
With 182 enzymes: SgfI *
December 21, 2016 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 364 591
Size: 364 227
AlwNI CAG_nnn'CTG
Cuts at: 0 324 327 349 591
Size: 324 3 22 242
Fragments arranged by size:
324 242 22 3
ApaLI G'TGCA_C
Cuts at: 0 91 591
Size: 91 500
AvaI C'yCGr_G
Cuts at: 0 59 591
Size: 59 532
BanI G'GyrC_C
Cuts at: 0 222 352 573 591
Size: 222 130 221 18
Fragments arranged by size:
222 221 130 18
BanII G_rGCy'C
Cuts at: 0 37 159 591
Size: 37 122 432
Fragments arranged by size:
432 122 37
BbeI G_GCGC'C
Cuts at: 0 226 591
Size: 226 365
BglI GCCn_nnn'nGGC
Cuts at: 0 71 591
Size: 71 520
BlpI GC'TnA_GC
Cuts at: 0 510 591
Size: 510 81
Bme1580I G_kGCm'C
Cuts at: 0 95 591
Size: 95 496
BmrI ACTGGGnnnn_n'
Cuts at: 0 82 389 591
Size: 82 307 202
Fragments arranged by size:
307 202 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 178 591
Size: 178 413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 365 591
Size: 226 139 226
Fragments arranged by size:
226 226 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 591
Size: 421 170
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 591
Size: 175 416
BsaHI Gr'CG_yC
Cuts at: 0 223 591
Size: 223 368
BsaWI w'CCGG_w
Cuts at: 0 520 591
Size: 520 71
BseYI C'CCAG_C
Cuts at: 0 233 480 591
Size: 233 247 111
Fragments arranged by size:
247 233 111
BsiHKAI G_wGCw'C
Cuts at: 0 95 260 591
Size: 95 165 331
Fragments arranged by size:
331 165 95
BsmI GAATG_Cn'
Cuts at: 0 74 445 591
Size: 74 371 146
Fragments arranged by size:
371 146 74
Bsp1286I G_dGCh'C
Cuts at: 0 37 95 159 260 591
Size: 37 58 64 101 331
Fragments arranged by size:
331 101 64 58 37
BspEI T'CCGG_A
Cuts at: 0 520 591
Size: 520 71
BspHI T'CATG_A
Cuts at: 0 368 591
Size: 368 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 364 591
Size: 364 227
BsrBI CCG'CTC
Cuts at: 0 579 591
Size: 579 12
BssSI C'ACGA_G
Cuts at: 0 301 591
Size: 301 290
BstAPI GCAn_nnn'nTGC
Cuts at: 0 80 359 591
Size: 80 279 232
Fragments arranged by size:
279 232 80
BstEII G'GTnAC_C
Cuts at: 0 264 591
Size: 264 327
BtsI GCAGTG_nn'
Cuts at: 0 269 470 591
Size: 269 201 121
Fragments arranged by size:
269 201 121
DraIII CAC_nnn'GTG
Cuts at: 0 563 591
Size: 563 28
EaeI y'GGCC_r
Cuts at: 0 548 591
Size: 548 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 365 591
Size: 226 139 226
Fragments arranged by size:
226 226 139
EcoO109I rG'GnC_Cy
Cuts at: 0 21 528 591
Size: 21 507 63
Fragments arranged by size:
507 63 21
HaeII r_GCGC'y
Cuts at: 0 226 591
Size: 226 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 591
Size: 493 32 66
Fragments arranged by size:
493 66 32
KasI G'GCGC_C
Cuts at: 0 222 591
Size: 222 369
MslI CAynn'nnrTG
Cuts at: 0 139 196 550 591
Size: 139 57 354 41
Fragments arranged by size:
354 139 57 41
MspA1I CmG'CkG
Cuts at: 0 324 591
Size: 324 267
NarI GG'CG_CC
Cuts at: 0 223 591
Size: 223 368
PflMI CCAn_nnn'nTGG
Cuts at: 0 349 591
Size: 349 242
PpuMI rG'GwC_Cy
Cuts at: 0 21 528 591
Size: 21 507 63
Fragments arranged by size:
507 63 21
PstI C_TGCA'G
Cuts at: 0 452 591
Size: 452 139
PvuII CAG'CTG
Cuts at: 0 324 591
Size: 324 267
SanDI GG'GwC_CC
Cuts at: 0 528 591
Size: 528 63
SexAI A'CCwGG_T
Cuts at: 0 260 591
Size: 260 331
SfcI C'TryA_G
Cuts at: 0 448 591
Size: 448 143
SfoI GGC'GCC
Cuts at: 0 224 591
Size: 224 367
SmaI CCC'GGG
Cuts at: 0 61 591
Size: 61 530
SmlI C'TyrA_G
Cuts at: 0 400 591
Size: 400 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 591
Size: 300 291
XmaI C'CCGG_G
Cuts at: 0 59 591
Size: 59 532
Enzymes that do cut:
AarI AlwNI ApaLI AvaI BanI BanII BbeI BglI
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI
BsaWI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BssSI BstAPI BstEII BtsI DraIII EaeI Eco57MI
EcoO109I HaeII Hin4I KasI MslI MspA1I NarI PflMI
PpuMI PstI PvuII SanDI SexAI SfcI SfoI SmaI
SmlI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I BsaAI
BsaBI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BsrDI
BsrFI BsrGI BssHII BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI
ZraI