(Linear) (Six-base) MAPSORT of: KIEE2656.seq  Check: 9293  from: 1  to: 792



KIEE2656.orf

 With 182 enzymes: SgfI * 

                        December 1, 2016 14:58  ..

Acc65I G'GTAC_C

Cuts at:      0     419     792 
   Size:        419     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     667     792 
   Size:        245     422     125

  Fragments arranged by size: 

                 422     245     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0      67     341     721     792 
   Size:         67     274     380      71

  Fragments arranged by size: 

                 380     274      71      67
 
ApoI r'AATT_y

Cuts at:      0     198     616     792 
   Size:        198     418     176

  Fragments arranged by size: 

                 418     198     176
 
BanI G'GyrC_C

Cuts at:      0     419     792 
   Size:        419     373
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     766     792 
   Size:        766      26
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     544     792 
   Size:        544     248
 
BfrBI ATG'CAT

Cuts at:      0     649     792 
   Size:        649     143
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     486     792 
   Size:        486     306
 
BsaWI w'CCGG_w

Cuts at:      0     416     792 
   Size:        416     376
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     563     792 
   Size:        563     229
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     767     792 
   Size:        767      25
 
BsmI GAATG_Cn'

Cuts at:      0     503     539     792 
   Size:        503      36     253

  Fragments arranged by size: 

                 503     253      36
 
BsrDI GCAATG_nn'

Cuts at:      0      98     235     792 
   Size:         98     137     557

  Fragments arranged by size: 

                 557     137      98
 
BsrFI r'CCGG_y

Cuts at:      0      37     497     792 
   Size:         37     460     295

  Fragments arranged by size: 

                 460     295      37
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     749     792 
   Size:        749      43
 
BstYI r'GATC_y

Cuts at:      0      96     271     792 
   Size:         96     175     521

  Fragments arranged by size: 

                 521     175      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     719     792 
   Size:        719      73
 
BtsI GCAGTG_nn'

Cuts at:      0     342     774     792 
   Size:        342     432      18

  Fragments arranged by size: 

                 432     342      18
 
EaeI y'GGCC_r

Cuts at:      0      81     792 
   Size:         81     711
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     667     792 
   Size:        245     422     125

  Fragments arranged by size: 

                 422     245     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     539     571     792 
   Size:        539      32     221

  Fragments arranged by size: 

                 539     221      32
 
KpnI G_GTAC'C

Cuts at:      0     423     792 
   Size:        423     369
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     147     792 
   Size:         51      96     645

  Fragments arranged by size: 

                 645      96      51
 
MscI TGG'CCA

Cuts at:      0      83     792 
   Size:         83     709
 
NaeI GCC'GGC

Cuts at:      0      39     792 
   Size:         39     753
 
NdeI CA'TA_TG

Cuts at:      0     180     792 
   Size:        180     612
 
NgoMIV G'CCGG_C

Cuts at:      0      37     792 
   Size:         37     755
 
NsiI A_TGCA'T

Cuts at:      0     651     792 
   Size:        651     141
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     166     792 
   Size:        166     626
 
PfoI T'CCnGG_A

Cuts at:      0     543     792 
   Size:        543     249
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     341     373     792 
   Size:        341      32     419

  Fragments arranged by size: 

                 419     341      32
 
PstI C_TGCA'G

Cuts at:      0     398     792 
   Size:        398     394
 
ScaI AGT'ACT

Cuts at:      0     408     792 
   Size:        408     384
 
SfcI C'TryA_G

Cuts at:      0     262     394     792 
   Size:        262     132     398

  Fragments arranged by size: 

                 398     262     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     315     792 
   Size:        315     477
 
TatI w'GTAC_w

Cuts at:      0     406     792 
   Size:        406     386
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI     BbsI    BciVI    BfrBI
     BmrI    BsaWI    BseRI     BsgI     BsmI    BsrDI    BsrFI    BstXI
    BstYI    BtgZI     BtsI     EaeI  Eco57MI    Hin4I     KpnI     MmeI
     MscI     NaeI     NdeI   NgoMIV     NsiI    PflMI     PfoI     PpiI
     PstI     ScaI     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MslI   MspA1I     NarI     NcoI
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI