Restriction map for
pF1KE2655
(Linear) (Six-base) MAPSORT of: KIEE2655.seq Check: 5555 from: 1 to: 1323
KIEE2655.orf
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 0 926 1259 1323
Size: 926 333 64
Fragments arranged by size:
926 333 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 778 817 1323
Size: 329 449 39 506
Fragments arranged by size:
506 449 329 39
AflIII A'CryG_T
Cuts at: 0 894 1205 1323
Size: 894 311 118
Fragments arranged by size:
894 311 118
AhdI GACnn_n'nnGTC
Cuts at: 0 1293 1323
Size: 1293 30
AseI AT'TA_AT
Cuts at: 0 833 1323
Size: 833 490
AvaI C'yCGr_G
Cuts at: 0 659 900 1323
Size: 659 241 423
Fragments arranged by size:
659 423 241
BanII G_rGCy'C
Cuts at: 0 11 181 252 668 1323
Size: 11 170 71 416 655
Fragments arranged by size:
655 416 170 71 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 701 1323
Size: 350 351 622
Fragments arranged by size:
622 351 350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1279 1313 1323
Size: 1279 34 10
Fragments arranged by size:
1279 34 10
BglI GCCn_nnn'nGGC
Cuts at: 0 430 488 1306 1323
Size: 430 58 818 17
Fragments arranged by size:
818 430 58 17
BlpI GC'TnA_GC
Cuts at: 0 5 1323
Size: 5 1318
Bme1580I G_kGCm'C
Cuts at: 0 746 1323
Size: 746 577
BmgBI CAC'GTC
Cuts at: 0 897 1086 1208 1323
Size: 897 189 122 115
Fragments arranged by size:
897 189 122 115
BmtI G_CTAG'C
Cuts at: 0 1285 1323
Size: 1285 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1323
Size: 461 862
Bpu10I CC'TnA_GC
Cuts at: 0 943 1323
Size: 943 380
BsaAI yAC'GTr
Cuts at: 0 363 1323
Size: 363 960
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1162 1192 1323
Size: 1162 30 131
Fragments arranged by size:
1162 131 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1224 1323
Size: 1224 99
BseYI C'CCAG_C
Cuts at: 0 43 334 547 1268 1323
Size: 43 291 213 721 55
Fragments arranged by size:
721 291 213 55 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1323
Size: 844 479
BsiHKAI G_wGCw'C
Cuts at: 0 205 252 1323
Size: 205 47 1071
Fragments arranged by size:
1071 205 47
BsiWI C'GTAC_G
Cuts at: 0 50 1323
Size: 50 1273
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1214 1323
Size: 1214 109
Bsp1286I G_dGCh'C
Cuts at: 0 11 181 205 252 668 746 1323
Size: 11 170 24 47 416 78 577
Fragments arranged by size:
577 416 170 78 47 24 11
BsrBI CCG'CTC
Cuts at: 0 532 626 1323
Size: 532 94 697
Fragments arranged by size:
697 532 94
BsrDI GCAATG_nn'
Cuts at: 0 1233 1323
Size: 1233 90
BsrFI r'CCGG_y
Cuts at: 0 1240 1323
Size: 1240 83
BsrGI T'GTAC_A
Cuts at: 0 1178 1323
Size: 1178 145
BstBI TT'CG_AA
Cuts at: 0 23 1323
Size: 23 1300
BstEII G'GTnAC_C
Cuts at: 0 951 1323
Size: 951 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 1090 1323
Size: 675 415 233
Fragments arranged by size:
675 415 233
BstYI r'GATC_y
Cuts at: 0 163 287 846 1323
Size: 163 124 559 477
Fragments arranged by size:
559 477 163 124
BtgI C'CryG_G
Cuts at: 0 460 1161 1213 1323
Size: 460 701 52 110
Fragments arranged by size:
701 460 110 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 425 1323
Size: 425 898
BtsI GCAGTG_nn'
Cuts at: 0 686 918 1323
Size: 686 232 405
Fragments arranged by size:
686 405 232
EaeI y'GGCC_r
Cuts at: 0 1002 1307 1323
Size: 1002 305 16
Fragments arranged by size:
1002 305 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 702 1109 1183 1323
Size: 702 407 74 140
Fragments arranged by size:
702 407 140 74
EcoICRI GAG'CTC
Cuts at: 0 250 1323
Size: 250 1073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 461 778 817 1323
Size: 329 132 317 39 506
Fragments arranged by size:
506 329 317 132 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 1094 1323
Size: 946 148 229
Fragments arranged by size:
946 229 148
EcoO109I rG'GnC_Cy
Cuts at: 0 1064 1323
Size: 1064 259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1050 1146 1323
Size: 1050 96 177
Fragments arranged by size:
1050 177 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 148 180 357 389 1279 1311
Size: 21 32 95 32 177 32 890 32
Cuts at: 1311 1323
Size: 12
Fragments arranged by size:
890 177 95 32 32 32 32 21
12
HincII GTy'rAC
Cuts at: 0 939 1323
Size: 939 384
HindIII A'AGCT_T
Cuts at: 0 1027 1323
Size: 1027 296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 670 1323
Size: 232 438 653
Fragments arranged by size:
653 438 232
MscI TGG'CCA
Cuts at: 0 1004 1309 1323
Size: 1004 305 14
Fragments arranged by size:
1004 305 14
MslI CAynn'nnrTG
Cuts at: 0 1252 1323
Size: 1252 71
MspA1I CmG'CkG
Cuts at: 0 214 307 462 584 1323
Size: 214 93 155 122 739
Fragments arranged by size:
739 214 155 122 93
NheI G'CTAG_C
Cuts at: 0 1281 1323
Size: 1281 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 1000 1323
Size: 1000 323
PmlI CAC'GTG
Cuts at: 0 363 1323
Size: 363 960
PpuMI rG'GwC_Cy
Cuts at: 0 1064 1323
Size: 1064 259
PstI C_TGCA'G
Cuts at: 0 146 731 1323
Size: 146 585 592
Fragments arranged by size:
592 585 146
PvuII CAG'CTG
Cuts at: 0 307 1323
Size: 307 1016
SacI G_AGCT'C
Cuts at: 0 252 1323
Size: 252 1071
SacII CC_GC'GG
Cuts at: 0 463 1323
Size: 463 860
SanDI GG'GwC_CC
Cuts at: 0 1064 1323
Size: 1064 259
SfcI C'TryA_G
Cuts at: 0 142 588 727 1323
Size: 142 446 139 596
Fragments arranged by size:
596 446 142 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 488 1323
Size: 488 835
SmaI CCC'GGG
Cuts at: 0 661 902 1323
Size: 661 241 421
Fragments arranged by size:
661 421 241
StyI C'CwwG_G
Cuts at: 0 425 1323
Size: 425 898
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 561 669 711 1096 1323
Size: 561 108 42 385 227
Fragments arranged by size:
561 385 227 108 42
TatI w'GTAC_w
Cuts at: 0 1178 1323
Size: 1178 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 495 527 1323
Size: 495 32 796
Fragments arranged by size:
796 495 32
Tth111I GACn'n_nGTC
Cuts at: 0 1045 1323
Size: 1045 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 868 1057 1323
Size: 868 189 266
Fragments arranged by size:
868 266 189
XmaI C'CCGG_G
Cuts at: 0 659 900 1323
Size: 659 241 423
Fragments arranged by size:
659 423 241
Enzymes that do cut:
AccI AcuI AflIII AhdI AseI AvaI BanII BbsI
BcgI BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NheI PflMI PmlI PpuMI PstI PvuII
SacI SacII SanDI SfcI SfiI SmaI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BglII BmrI BplI BpuEI BsaI BsaBI BsaHI
BsaWI BsiEI BsmI BspEI BspHI BspMI BssHII BssSI
BstAPI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EarI EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XhoI XmnI ZraI