Restriction map for
pF1KE2654
(Linear) (Six-base) MAPSORT of: KIEE2654.seq Check: 6533 from: 1 to: 837
KIEE2654.orf
With 182 enzymes: SgfI *
December 1, 2016 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 396 837
Size: 396 441
AccI GT'mk_AC
Cuts at: 0 191 837
Size: 191 646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 837
Size: 778 59
AflIII A'CryG_T
Cuts at: 0 113 420 837
Size: 113 307 417
Fragments arranged by size:
417 307 113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 837
Size: 796 41
ApaLI G'TGCA_C
Cuts at: 0 788 837
Size: 788 49
AvaI C'yCGr_G
Cuts at: 0 477 837
Size: 477 360
BamHI G'GATC_C
Cuts at: 0 53 837
Size: 53 784
BanI G'GyrC_C
Cuts at: 0 718 837
Size: 718 119
BanII G_rGCy'C
Cuts at: 0 493 810 837
Size: 493 317 27
Fragments arranged by size:
493 317 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 417 837
Size: 417 420
BlpI GC'TnA_GC
Cuts at: 0 256 837
Size: 256 581
Bme1580I G_kGCm'C
Cuts at: 0 792 837
Size: 792 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 682 837
Size: 645 37 155
Fragments arranged by size:
645 155 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 794 837
Size: 468 326 43
Fragments arranged by size:
468 326 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 639 837
Size: 639 198
BsaAI yAC'GTr
Cuts at: 0 114 837
Size: 114 723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 837
Size: 625 212
BsiHKAI G_wGCw'C
Cuts at: 0 262 792 810 837
Size: 262 530 18 27
Fragments arranged by size:
530 262 27 18
Bsp1286I G_dGCh'C
Cuts at: 0 262 493 792 810 837
Size: 262 231 299 18 27
Fragments arranged by size:
299 262 231 27 18
BspHI T'CATG_A
Cuts at: 0 182 837
Size: 182 655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 396 837
Size: 396 441
BstEII G'GTnAC_C
Cuts at: 0 408 837
Size: 408 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 837
Size: 175 662
BstYI r'GATC_y
Cuts at: 0 45 53 837
Size: 45 8 784
Fragments arranged by size:
784 45 8
Bsu36I CC'TnA_GG
Cuts at: 0 305 837
Size: 305 532
BtgI C'CryG_G
Cuts at: 0 174 837
Size: 174 663
BtsI GCAGTG_nn'
Cuts at: 0 637 837
Size: 637 200
DraIII CAC_nnn'GTG
Cuts at: 0 438 837
Size: 438 399
EarI CTCTTCn'nnn_
Cuts at: 0 343 349 519 837
Size: 343 6 170 318
Fragments arranged by size:
343 318 170 6
EcoICRI GAG'CTC
Cuts at: 0 808 837
Size: 808 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 778 794 837
Size: 468 310 16 43
Fragments arranged by size:
468 310 43 16
EcoO109I rG'GnC_Cy
Cuts at: 0 77 837
Size: 77 760
HaeII r_GCGC'y
Cuts at: 0 276 837
Size: 276 561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 767 837
Size: 320 447 70
Fragments arranged by size:
447 320 70
MslI CAynn'nnrTG
Cuts at: 0 709 837
Size: 709 128
MspA1I CmG'CkG
Cuts at: 0 615 671 837
Size: 615 56 166
Fragments arranged by size:
615 166 56
NspI r_CATG'y
Cuts at: 0 424 526 837
Size: 424 102 311
Fragments arranged by size:
424 311 102
PciI A'CATG_T
Cuts at: 0 420 837
Size: 420 417
PflMI CCAn_nnn'nTGG
Cuts at: 0 749 837
Size: 749 88
PpuMI rG'GwC_Cy
Cuts at: 0 77 837
Size: 77 760
PvuII CAG'CTG
Cuts at: 0 615 671 837
Size: 615 56 166
Fragments arranged by size:
615 166 56
SacI G_AGCT'C
Cuts at: 0 810 837
Size: 810 27
SanDI GG'GwC_CC
Cuts at: 0 77 837
Size: 77 760
SapI GCTCTTCn'nnn_
Cuts at: 0 343 837
Size: 343 494
ScaI AGT'ACT
Cuts at: 0 655 837
Size: 655 182
SmaI CCC'GGG
Cuts at: 0 479 837
Size: 479 358
StyI C'CwwG_G
Cuts at: 0 767 821 837
Size: 767 54 16
Fragments arranged by size:
767 54 16
TatI w'GTAC_w
Cuts at: 0 116 539 653 837
Size: 116 423 114 184
Fragments arranged by size:
423 184 116 114
XmaI C'CCGG_G
Cuts at: 0 477 837
Size: 477 360
XmnI GAAnn'nnTTC
Cuts at: 0 163 837
Size: 163 674
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI ApaLI AvaI BamHI
BanI BanII BbsI BlpI Bme1580I BmrI BpmI BsaI
BsaAI BsgI BsiHKAI Bsp1286I BspHI BspMI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EarI EcoICRI Eco57MI
EcoO109I HaeII MmeI MslI MspA1I NspI PciI PflMI
PpuMI PvuII SacI SanDI SapI ScaI SmaI StyI
TatI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
ZraI