Restriction map for
pF1KE2648
(Linear) (Six-base) MAPSORT of: KIEE2648.seq Check: 8630 from: 1 to: 2400
KIEE2648.orf
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 0 469 2270 2400
Size: 469 1801 130
Fragments arranged by size:
1801 469 130
Acc65I G'GTAC_C
Cuts at: 0 792 2400
Size: 792 1608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1594 1609 2325 2400
Size: 1403 191 15 716 75
Fragments arranged by size:
1403 716 191 75 15
AflIII A'CryG_T
Cuts at: 0 315 2400
Size: 315 2085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 658 1585 2400
Size: 658 927 815
Fragments arranged by size:
927 815 658
AlwNI CAG_nnn'CTG
Cuts at: 0 75 585 1177 1836 2400
Size: 75 510 592 659 564
Fragments arranged by size:
659 592 564 510 75
ApoI r'AATT_y
Cuts at: 0 198 1159 1201 1322 1841 1906 2335 2400
Size: 198 961 42 121 519 65 429 65
Fragments arranged by size:
961 519 429 198 121 65 65 42
AseI AT'TA_AT
Cuts at: 0 2170 2224 2400
Size: 2170 54 176
Fragments arranged by size:
2170 176 54
AvaI C'yCGr_G
Cuts at: 0 33 2400
Size: 33 2367
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 275 308 2400
Size: 275 33 2092
Fragments arranged by size:
2092 275 33
BamHI G'GATC_C
Cuts at: 0 2023 2400
Size: 2023 377
BanI G'GyrC_C
Cuts at: 0 792 2400
Size: 792 1608
BanII G_rGCy'C
Cuts at: 0 935 1136 2400
Size: 935 201 1264
Fragments arranged by size:
1264 935 201
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 2400
Size: 233 2167
BbvCI CC'TCA_GC
Cuts at: 0 2064 2400
Size: 2064 336
BciVI GTATCCnnnnn_n'
Cuts at: 0 842 860 866 2400
Size: 842 18 6 1534
Fragments arranged by size:
1534 842 18 6
BfrBI ATG'CAT
Cuts at: 0 1406 2400
Size: 1406 994
BglII A'GATC_T
Cuts at: 0 1292 2400
Size: 1292 1108
BmgBI CAC'GTC
Cuts at: 0 1195 2400
Size: 1195 1205
BmrI ACTGGGnnnn_n'
Cuts at: 0 85 1287 1501 1770 2400
Size: 85 1202 214 269 630
Fragments arranged by size:
1202 630 269 214 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 842 2267 2400
Size: 512 330 1425 133
Fragments arranged by size:
1425 512 330 133
Bpu10I CC'TnA_GC
Cuts at: 0 2064 2400
Size: 2064 336
BsaI GGTCTCn'nnnn_
Cuts at: 0 1054 2400
Size: 1054 1346
BsaAI yAC'GTr
Cuts at: 0 1109 2400
Size: 1109 1291
BsaBI GATnn'nnATC
Cuts at: 0 1291 2400
Size: 1291 1109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1123 1153 1918 1948 2400
Size: 1123 30 765 30 452
Fragments arranged by size:
1123 765 452 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1144 2400
Size: 1144 1256
BseYI C'CCAG_C
Cuts at: 0 1536 2400
Size: 1536 864
BsiEI CG_ry'CG
Cuts at: 0 8 2400
Size: 8 2392
BsiHKAI G_wGCw'C
Cuts at: 0 935 1339 2400
Size: 935 404 1061
Fragments arranged by size:
1061 935 404
BsmI GAATG_Cn'
Cuts at: 0 720 2400
Size: 720 1680
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1142 2400
Size: 1142 1258
Bsp1286I G_dGCh'C
Cuts at: 0 935 1136 1339 2400
Size: 935 201 203 1061
Fragments arranged by size:
1061 935 203 201
BspHI T'CATG_A
Cuts at: 0 1421 2400
Size: 1421 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 856 2256 2400
Size: 856 1400 144
Fragments arranged by size:
1400 856 144
BsrDI GCAATG_nn'
Cuts at: 0 640 2400
Size: 640 1760
BsrFI r'CCGG_y
Cuts at: 0 60 2400
Size: 60 2340
BsrGI T'GTAC_A
Cuts at: 0 1367 1435 2400
Size: 1367 68 965
Fragments arranged by size:
1367 965 68
BstEII G'GTnAC_C
Cuts at: 0 743 2400
Size: 743 1657
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1008 2400
Size: 1008 1392
BstYI r'GATC_y
Cuts at: 0 1292 2023 2400
Size: 1292 731 377
Fragments arranged by size:
1292 731 377
BstZ17I GTA'TAC
Cuts at: 0 470 2400
Size: 470 1930
Bsu36I CC'TnA_GG
Cuts at: 0 860 2400
Size: 860 1540
BtgI C'CryG_G
Cuts at: 0 800 2027 2400
Size: 800 1227 373
Fragments arranged by size:
1227 800 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 714 2400
Size: 714 1686
DraI TTT'AAA
Cuts at: 0 419 954 2016 2400
Size: 419 535 1062 384
Fragments arranged by size:
1062 535 419 384
EaeI y'GGCC_r
Cuts at: 0 5 2400
Size: 5 2395
EagI C'GGCC_G
Cuts at: 0 5 2400
Size: 5 2395
EarI CTCTTCn'nnn_
Cuts at: 0 701 1703 2296 2400
Size: 701 1002 593 104
Fragments arranged by size:
1002 701 593 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 2400
Size: 79 2321
EcoICRI GAG'CTC
Cuts at: 0 933 2400
Size: 933 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 842 1403 1594 1609 2267 2325 2400
Size: 512 330 561 191 15 658 58 75
Fragments arranged by size:
658 561 512 330 191 75 58 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 863 2400
Size: 863 1537
EcoO109I rG'GnC_Cy
Cuts at: 0 1503 2400
Size: 1503 897
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1084 1116 2295 2327 2400
Size: 1084 32 1179 32 73
Fragments arranged by size:
1179 1084 73 32 32
HindIII A'AGCT_T
Cuts at: 0 2008 2400
Size: 2008 392
KpnI G_GTAC'C
Cuts at: 0 796 2400
Size: 796 1604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2232 2400
Size: 2232 168
MslI CAynn'nnrTG
Cuts at: 0 160 1216 1780 2400
Size: 160 1056 564 620
Fragments arranged by size:
1056 620 564 160
MspA1I CmG'CkG
Cuts at: 0 585 1365 1734 1900 2400
Size: 585 780 369 166 500
Fragments arranged by size:
780 585 500 369 166
NaeI GCC'GGC
Cuts at: 0 62 2400
Size: 62 2338
NcoI C'CATG_G
Cuts at: 0 800 2400
Size: 800 1600
NdeI CA'TA_TG
Cuts at: 0 438 480 2400
Size: 438 42 1920
Fragments arranged by size:
1920 438 42
NgoMIV G'CCGG_C
Cuts at: 0 60 2400
Size: 60 2340
NsiI A_TGCA'T
Cuts at: 0 1408 2400
Size: 1408 992
NspI r_CATG'y
Cuts at: 0 319 2400
Size: 319 2081
PciI A'CATG_T
Cuts at: 0 315 2400
Size: 315 2085
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 2400
Size: 75 2325
PpuMI rG'GwC_Cy
Cuts at: 0 1503 2400
Size: 1503 897
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1634 1666 2400
Size: 1634 32 734
Fragments arranged by size:
1634 734 32
PvuII CAG'CTG
Cuts at: 0 585 1365 1734 1900 2400
Size: 585 780 369 166 500
Fragments arranged by size:
780 585 500 369 166
SacI G_AGCT'C
Cuts at: 0 935 2400
Size: 935 1465
SanDI GG'GwC_CC
Cuts at: 0 1503 2400
Size: 1503 897
SapI GCTCTTCn'nnn_
Cuts at: 0 1703 2296 2400
Size: 1703 593 104
Fragments arranged by size:
1703 593 104
SfcI C'TryA_G
Cuts at: 0 1067 2319 2400
Size: 1067 1252 81
Fragments arranged by size:
1252 1067 81
SmaI CCC'GGG
Cuts at: 0 35 2400
Size: 35 2365
SspI AAT'ATT
Cuts at: 0 1546 2400
Size: 1546 854
StyI C'CwwG_G
Cuts at: 0 573 800 1498 2085 2400
Size: 573 227 698 587 315
Fragments arranged by size:
698 587 573 315 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1697 2400
Size: 1697 703
TatI w'GTAC_w
Cuts at: 0 1367 1435 2400
Size: 1367 68 965
Fragments arranged by size:
1367 965 68
Tth111I GACn'n_nGTC
Cuts at: 0 544 2400
Size: 544 1856
XbaI T'CTAG_A
Cuts at: 0 1144 2400
Size: 1144 1256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 101 219 269 357 1674 2400
Size: 101 118 50 88 1317 726
Fragments arranged by size:
1317 726 118 101 88 50
XmaI C'CCGG_G
Cuts at: 0 33 2400
Size: 33 2367
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApoI AseI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI
BfrBI BglII BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI DraI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII
KpnI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PpuMI PsrI PvuII SacI
SanDI SapI SfcI SmaI SspI StyI TaqII TatI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BbeI BcgI
BclI BglI BlpI Bme1580I BmtI BplI BpuEI BsaHI
BsaWI BsgI BsiWI BspEI BsrBI BssHII BssSI BstAPI
BstBI BtsI ClaI CspCI DraIII DrdI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI MscI NarI NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TstI XhoI XmnI ZraI