Restriction map for
pF1KE2641
(Linear) (Six-base) MAPSORT of: KIEE2641.seq Check: 5991 from: 1 to: 1317
KIEE2641.orf
With 182 enzymes: SgfI *
October 5, 2016 15:26 ..
Acc65I G'GTAC_C
Cuts at: 0 462 1317
Size: 462 855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 673 811 1317
Size: 452 221 138 506
Fragments arranged by size:
506 452 221 138
AfeI AGC'GCT
Cuts at: 0 808 1317
Size: 808 509
AflIII A'CryG_T
Cuts at: 0 912 1167 1317
Size: 912 255 150
Fragments arranged by size:
912 255 150
ApoI r'AATT_y
Cuts at: 0 340 987 1317
Size: 340 647 330
Fragments arranged by size:
647 340 330
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 674 707 1317
Size: 674 33 610
Fragments arranged by size:
674 610 33
BanI G'GyrC_C
Cuts at: 0 462 931 1317
Size: 462 469 386
Fragments arranged by size:
469 462 386
BbsI GAAGACnn'nnnn_
Cuts at: 0 711 1317
Size: 711 606
BclI T'GATC_A
Cuts at: 0 2 594 1317
Size: 2 592 723
Fragments arranged by size:
723 592 2
Bme1580I G_kGCm'C
Cuts at: 0 934 1317
Size: 934 383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 45 724 1317
Size: 45 679 593
Fragments arranged by size:
679 593 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 878 1025 1201 1317
Size: 671 207 147 176 116
Fragments arranged by size:
671 207 176 147 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 787 1317
Size: 19 768 530
Fragments arranged by size:
768 530 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 1317
Size: 79 1238
BsaBI GATnn'nnATC
Cuts at: 0 208 1317
Size: 208 1109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 876 906 1023 1053 1199 1229 1317
Size: 876 30 117 30 146 30 88
Fragments arranged by size:
876 146 117 88 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 1317
Size: 34 1283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1317
Size: 893 424
BsiHKAI G_wGCw'C
Cuts at: 0 416 1317
Size: 416 901
BsmI GAATG_Cn'
Cuts at: 0 435 1292 1317
Size: 435 857 25
Fragments arranged by size:
857 435 25
Bsp1286I G_dGCh'C
Cuts at: 0 416 934 1317
Size: 416 518 383
Fragments arranged by size:
518 416 383
BspHI T'CATG_A
Cuts at: 0 351 561 1317
Size: 351 210 756
Fragments arranged by size:
756 351 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 1317
Size: 56 1261
BsrFI r'CCGG_y
Cuts at: 0 752 1317
Size: 752 565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 1317
Size: 69 1248
BstYI r'GATC_y
Cuts at: 0 372 1317
Size: 372 945
Bsu36I CC'TnA_GG
Cuts at: 0 88 441 1317
Size: 88 353 876
Fragments arranged by size:
876 353 88
BtgI C'CryG_G
Cuts at: 0 1129 1317
Size: 1129 188
EaeI y'GGCC_r
Cuts at: 0 246 1317
Size: 246 1071
EarI CTCTTCn'nnn_
Cuts at: 0 1223 1317
Size: 1223 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 671 673 811 878 1025 1201 1317
Size: 452 219 2 138 67 147 176 116
Fragments arranged by size:
452 219 176 147 138 116 67 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 470 698 1317
Size: 470 228 619
Fragments arranged by size:
619 470 228
EcoO109I rG'GnC_Cy
Cuts at: 0 1296 1317
Size: 1296 21
EcoRI G'AATT_C
Cuts at: 0 340 987 1317
Size: 340 647 330
Fragments arranged by size:
647 340 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 191 1317
Size: 191 1126
HaeII r_GCGC'y
Cuts at: 0 810 1317
Size: 810 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 283 315 487 519 876 908
Size: 57 32 194 32 172 32 357 32
Cuts at: 908 1317
Size: 409
Fragments arranged by size:
409 357 194 172 57 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1019 1173 1317
Size: 1019 154 144
Fragments arranged by size:
1019 154 144
HindIII A'AGCT_T
Cuts at: 0 556 1317
Size: 556 761
KpnI G_GTAC'C
Cuts at: 0 466 1317
Size: 466 851
MscI TGG'CCA
Cuts at: 0 248 1317
Size: 248 1069
MspA1I CmG'CkG
Cuts at: 0 850 1317
Size: 850 467
NcoI C'CATG_G
Cuts at: 0 1129 1317
Size: 1129 188
NspI r_CATG'y
Cuts at: 0 916 1171 1317
Size: 916 255 146
Fragments arranged by size:
916 255 146
PciI A'CATG_T
Cuts at: 0 912 1167 1317
Size: 912 255 150
Fragments arranged by size:
912 255 150
PshAI GACnn'nnGTC
Cuts at: 0 1042 1317
Size: 1042 275
PstI C_TGCA'G
Cuts at: 0 53 825 1317
Size: 53 772 492
Fragments arranged by size:
772 492 53
PvuII CAG'CTG
Cuts at: 0 850 1317
Size: 850 467
SapI GCTCTTCn'nnn_
Cuts at: 0 1223 1317
Size: 1223 94
SfcI C'TryA_G
Cuts at: 0 49 821 1317
Size: 49 772 496
Fragments arranged by size:
772 496 49
SmlI C'TyrA_G
Cuts at: 0 34 802 1317
Size: 34 768 515
Fragments arranged by size:
768 515 34
StyI C'CwwG_G
Cuts at: 0 782 1129 1317
Size: 782 347 188
Fragments arranged by size:
782 347 188
TatI w'GTAC_w
Cuts at: 0 38 1317
Size: 38 1279
Tth111I GACn'n_nGTC
Cuts at: 0 921 1317
Size: 921 396
XbaI T'CTAG_A
Cuts at: 0 15 1317
Size: 15 1302
Enzymes that do cut:
Acc65I AcuI AfeI AflIII ApoI BaeI BanI BbsI
BclI Bme1580I BplI BpmI BpuEI BsaI BsaBI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BstXI BstYI Bsu36I BtgI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KpnI
MscI MspA1I NcoI NspI PciI PshAI PstI PvuII
SapI SfcI SmlI StyI TatI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HpaI
KasI MfeI MluI MmeI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
XcmI XhoI XmaI XmnI ZraI