Restriction map for
pF1KE2639
(Linear) (Six-base) MAPSORT of: KIEE2639.seq Check: 7279 from: 1 to: 2160
KIEE2639.orf
With 182 enzymes: SgfI *
September 8, 2016 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 98 1059 1353 2160
Size: 98 961 294 807
Fragments arranged by size:
961 807 294 98
AccI GT'mk_AC
Cuts at: 0 446 656 1109 2147 2160
Size: 446 210 453 1038 13
Fragments arranged by size:
1038 453 446 210 13
Acc65I G'GTAC_C
Cuts at: 0 341 2160
Size: 341 1819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 604 805 1393 1411 2160
Size: 117 487 201 588 18 749
Fragments arranged by size:
749 588 487 201 117 18
AflIII A'CryG_T
Cuts at: 0 1592 2160
Size: 1592 568
AhdI GACnn_n'nnGTC
Cuts at: 0 1209 2160
Size: 1209 951
AleI CACnn'nnGTG
Cuts at: 0 301 2160
Size: 301 1859
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 2160
Size: 1672 32 456
Fragments arranged by size:
1672 456 32
AlwNI CAG_nnn'CTG
Cuts at: 0 260 1074 1389 1641 1761 2160
Size: 260 814 315 252 120 399
Fragments arranged by size:
814 399 315 260 252 120
ApaLI G'TGCA_C
Cuts at: 0 855 2160
Size: 855 1305
ApoI r'AATT_y
Cuts at: 0 1528 2160
Size: 1528 632
AseI AT'TA_AT
Cuts at: 0 1871 2160
Size: 1871 289
AvaI C'yCGr_G
Cuts at: 0 218 2160
Size: 218 1942
BamHI G'GATC_C
Cuts at: 0 524 878 1345 2160
Size: 524 354 467 815
Fragments arranged by size:
815 524 467 354
BanI G'GyrC_C
Cuts at: 0 59 341 428 745 2160
Size: 59 282 87 317 1415
Fragments arranged by size:
1415 317 282 87 59
BanII G_rGCy'C
Cuts at: 0 43 2160
Size: 43 2117
BbeI G_GCGC'C
Cuts at: 0 749 2160
Size: 749 1411
BbsI GAAGACnn'nnnn_
Cuts at: 0 566 1998 2160
Size: 566 1432 162
Fragments arranged by size:
1432 566 162
BciVI GTATCCnnnnn_n'
Cuts at: 0 984 2160
Size: 984 1176
BclI T'GATC_A
Cuts at: 0 683 893 935 2160
Size: 683 210 42 1225
Fragments arranged by size:
1225 683 210 42
Bme1580I G_kGCm'C
Cuts at: 0 433 859 1635 1794 2160
Size: 433 426 776 159 366
Fragments arranged by size:
776 433 426 366 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 1496 1596 1629 1740 2160
Size: 1496 100 33 111 420
Fragments arranged by size:
1496 420 111 100 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 27 295 744 1336 2071 2160
Size: 19 8 268 449 592 735 89
Fragments arranged by size:
735 592 449 268 89 19 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 271 2047 2160
Size: 271 1776 113
Fragments arranged by size:
1776 271 113
BsaBI GATnn'nnATC
Cuts at: 0 1783 2160
Size: 1783 377
BsaHI Gr'CG_yC
Cuts at: 0 746 1079 1106 2160
Size: 746 333 27 1054
Fragments arranged by size:
1054 746 333 27
BsaWI w'CCGG_w
Cuts at: 0 140 2160
Size: 140 2020
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 1582 1612 1686 1716 1874 1904
Size: 349 30 1203 30 74 30 158 30
Cuts at: 1904 2160
Size: 256
Fragments arranged by size:
1203 349 256 158 74 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 308 1436 2160
Size: 85 223 1128 724
Fragments arranged by size:
1128 724 223 85
BseYI C'CCAG_C
Cuts at: 0 253 621 833 2160
Size: 253 368 212 1327
Fragments arranged by size:
1327 368 253 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1630 2160
Size: 589 1041 530
Fragments arranged by size:
1041 589 530
BsiEI CG_ry'CG
Cuts at: 0 332 2160
Size: 332 1828
BsiHKAI G_wGCw'C
Cuts at: 0 43 639 713 859 2160
Size: 43 596 74 146 1301
Fragments arranged by size:
1301 596 146 74 43
BsmI GAATG_Cn'
Cuts at: 0 1203 2160
Size: 1203 957
Bsp1286I G_dGCh'C
Cuts at: 0 43 433 639 713 859 1635 1794 2160
Size: 43 390 206 74 146 776 159 366
Fragments arranged by size:
776 390 366 206 159 146 74 43
BspHI T'CATG_A
Cuts at: 0 482 2160
Size: 482 1678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 1059 1353 1935 2160
Size: 98 961 294 582 225
Fragments arranged by size:
961 582 294 225 98
BsrBI CCG'CTC
Cuts at: 0 1308 2160
Size: 1308 852
BsrDI GCAATG_nn'
Cuts at: 0 699 835 2160
Size: 699 136 1325
Fragments arranged by size:
1325 699 136
BssSI C'ACGA_G
Cuts at: 0 1257 2160
Size: 1257 903
BstXI CCAn_nnnn'nTGG
Cuts at: 0 539 1509 2160
Size: 539 970 651
Fragments arranged by size:
970 651 539
BstYI r'GATC_y
Cuts at: 0 524 878 1041 1345 2084 2160
Size: 524 354 163 304 739 76
Fragments arranged by size:
739 524 354 304 163 76
Bsu36I CC'TnA_GG
Cuts at: 0 1088 2160
Size: 1088 1072
BtgI C'CryG_G
Cuts at: 0 332 1407 2160
Size: 332 1075 753
Fragments arranged by size:
1075 753 332
BtsI GCAGTG_nn'
Cuts at: 0 796 2160
Size: 796 1364
EaeI y'GGCC_r
Cuts at: 0 329 776 912 2160
Size: 329 447 136 1248
Fragments arranged by size:
1248 447 329 136
EagI C'GGCC_G
Cuts at: 0 329 2160
Size: 329 1831
EarI CTCTTCn'nnn_
Cuts at: 0 1796 1990 2160
Size: 1796 194 170
Fragments arranged by size:
1796 194 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 517 2160
Size: 517 1643
EcoICRI GAG'CTC
Cuts at: 0 41 2160
Size: 41 2119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 27 117 295 604 744 805 1336
Size: 19 8 90 178 309 140 61 531
Cuts at: 1336 1393 1411 2071 2160
Size: 57 18 660 89
Fragments arranged by size:
660 531 309 178 140 90 89 61
57 19 18 8
EcoO109I rG'GnC_Cy
Cuts at: 0 1611 2160
Size: 1611 549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2026 2160
Size: 2026 134
HaeII r_GCGC'y
Cuts at: 0 749 2160
Size: 749 1411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 855 887 1195 1227 2160
Size: 855 32 308 32 933
Fragments arranged by size:
933 855 308 32 32
HincII GTy'rAC
Cuts at: 0 1766 2160
Size: 1766 394
HindIII A'AGCT_T
Cuts at: 0 1243 1401 2160
Size: 1243 158 759
Fragments arranged by size:
1243 759 158
KasI G'GCGC_C
Cuts at: 0 745 2160
Size: 745 1415
KpnI G_GTAC'C
Cuts at: 0 345 2160
Size: 345 1815
MscI TGG'CCA
Cuts at: 0 778 914 2160
Size: 778 136 1246
Fragments arranged by size:
1246 778 136
MslI CAynn'nnrTG
Cuts at: 0 175 301 862 1915 2160
Size: 175 126 561 1053 245
Fragments arranged by size:
1053 561 245 175 126
MspA1I CmG'CkG
Cuts at: 0 195 2160
Size: 195 1965
NarI GG'CG_CC
Cuts at: 0 746 2160
Size: 746 1414
NcoI C'CATG_G
Cuts at: 0 1407 2160
Size: 1407 753
NdeI CA'TA_TG
Cuts at: 0 1056 2160
Size: 1056 1104
NspI r_CATG'y
Cuts at: 0 1212 1596 2160
Size: 1212 384 564
Fragments arranged by size:
1212 564 384
PasI CC'CwG_GG
Cuts at: 0 1794 2160
Size: 1794 366
PciI A'CATG_T
Cuts at: 0 1592 2160
Size: 1592 568
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 2160
Size: 260 1900
PfoI T'CCnGG_A
Cuts at: 0 1352 2160
Size: 1352 808
PpuMI rG'GwC_Cy
Cuts at: 0 1611 2160
Size: 1611 549
PstI C_TGCA'G
Cuts at: 0 1385 2160
Size: 1385 775
SacI G_AGCT'C
Cuts at: 0 43 2160
Size: 43 2117
ScaI AGT'ACT
Cuts at: 0 949 2160
Size: 949 1211
SfcI C'TryA_G
Cuts at: 0 1381 2160
Size: 1381 779
SfoI GGC'GCC
Cuts at: 0 747 2160
Size: 747 1413
StuI AGG'CCT
Cuts at: 0 112 158 2160
Size: 112 46 2002
Fragments arranged by size:
2002 112 46
StyI C'CwwG_G
Cuts at: 0 6 209 323 507 1407 2010 2160
Size: 6 203 114 184 900 603 150
Fragments arranged by size:
900 603 203 184 150 114 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1786 2160
Size: 1786 374
TatI w'GTAC_w
Cuts at: 0 128 947 1748 2160
Size: 128 819 801 412
Fragments arranged by size:
819 801 412 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 1686 1718 2160
Size: 349 32 1305 32 442
Fragments arranged by size:
1305 442 349 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 922 2160
Size: 330 592 1238
Fragments arranged by size:
1238 592 330
XmnI GAAnn'nnTTC
Cuts at: 0 2005 2160
Size: 2005 155
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AloI
AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI Bme1580I BmrI BpmI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI
BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
HindIII KasI KpnI MscI MslI MspA1I NarI NcoI
NdeI NspI PasI PciI PflMI PfoI PpuMI PstI
SacI ScaI SfcI SfoI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AlfI ApaI AscI
AsiSI AvrII BaeI BbvCI BcgI BfrBI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsiWI
BsmBI BspEI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI
EcoRI EcoRV FseI FspI FspAI HpaI MfeI MluI
MmeI NaeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI