(Linear) (Six-base) MAPSORT of: KIEE2638.seq  Check: 8630  from: 1  to: 792



KIEE2638.orf

 With 182 enzymes: SgfI * 

                        September 8, 2016 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718     792 
   Size:        718      74
 
AfeI AGC'GCT

Cuts at:      0      68     792 
   Size:         68     724
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     286     792 
   Size:        286     506
 
ApoI r'AATT_y

Cuts at:      0     634     792 
   Size:        634     158
 
AvaI C'yCGr_G

Cuts at:      0     416     792 
   Size:        416     376
 
BbvCI CC'TCA_GC

Cuts at:      0      82     792 
   Size:         82     710
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     134     792 
   Size:        112      22     658

  Fragments arranged by size: 

                 658     112      22
 
Bpu10I CC'TnA_GC

Cuts at:      0      82     792 
   Size:         82     710
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     555     688     792 
   Size:        377     178     133     104

  Fragments arranged by size: 

                 377     178     133     104
 
BsaAI yAC'GTr

Cuts at:      0     738     792 
   Size:        738      54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     397     427     792 
   Size:        397      30     365

  Fragments arranged by size: 

                 397     365      30
 
BseYI C'CCAG_C

Cuts at:      0     331     792 
   Size:        331     461
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     413     792 
   Size:        251     162     379

  Fragments arranged by size: 

                 379     251     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     251     413     792 
   Size:        251     162     379

  Fragments arranged by size: 

                 379     251     162
 
DraI TTT'AAA

Cuts at:      0      17     303     792 
   Size:         17     286     489

  Fragments arranged by size: 

                 489     286      17
 
EaeI y'GGCC_r

Cuts at:      0     587     792 
   Size:        587     205
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     792 
   Size:        356     436
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     481     792 
   Size:        481     311
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     134     718     792 
   Size:        112      22     584      74

  Fragments arranged by size: 

                 584     112      74      22
 
HaeII r_GCGC'y

Cuts at:      0      70     792 
   Size:         70     722
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     792 
   Size:        395     397
 
MscI TGG'CCA

Cuts at:      0     589     792 
   Size:        589     203
 
MspA1I CmG'CkG

Cuts at:      0     331     792 
   Size:        331     461
 
PvuII CAG'CTG

Cuts at:      0     331     792 
   Size:        331     461
 
SmaI CCC'GGG

Cuts at:      0     418     792 
   Size:        418     374
 
SmlI C'TyrA_G

Cuts at:      0     392     534     667     792 
   Size:        392     142     133     125

  Fragments arranged by size: 

                 392     142     133     125
 
SspI AAT'ATT

Cuts at:      0     549     792 
   Size:        549     243
 
StuI AGG'CCT

Cuts at:      0     398     792 
   Size:        398     394
 
StyI C'CwwG_G

Cuts at:      0     399     792 
   Size:        399     393
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     438     792 
   Size:        438     354
 
XmaI C'CCGG_G

Cuts at:      0     416     792 
   Size:        416     376
 
XmnI GAAnn'nnTTC

Cuts at:      0     487     548     792 
   Size:        487      61     244

  Fragments arranged by size: 

                 487     244      61
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI     ApoI     AvaI    BbvCI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaXI    BseYI  BsiHKAI Bsp1286I     DraI     EaeI
     EarI     EciI  Eco57MI    HaeII     MmeI     MscI   MspA1I    PvuII
     SmaI     SmlI     SspI     StuI     StyI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     ZraI