(Linear) (Six-base) MAPSORT of: KIEE2636.seq  Check: 7383  from: 1  to: 1062



KIEE2636.orf

 With 182 enzymes: SgfI * 

                        August 9, 2016 17:37  ..

AatII G_ACGT'C

Cuts at:      0     395    1062 
   Size:        395     667
 
AccI GT'mk_AC

Cuts at:      0     954    1062 
   Size:        954     108
 
AgeI A'CCGG_T

Cuts at:      0      90    1062 
   Size:         90     972
 
AhdI GACnn_n'nnGTC

Cuts at:      0     426    1062 
   Size:        426     636
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     572    1062 
   Size:        572     490
 
ApaI G_GGCC'C

Cuts at:      0     939    1062 
   Size:        939     123
 
ApoI r'AATT_y

Cuts at:      0     178     489     778    1062 
   Size:        178     311     289     284

  Fragments arranged by size: 

                 311     289     284     178
 
AvaI C'yCGr_G

Cuts at:      0     319    1062 
   Size:        319     743
 
BanII G_rGCy'C

Cuts at:      0     103     636     939    1062 
   Size:        103     533     303     123

  Fragments arranged by size: 

                 533     303     123     103
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     416    1062 
   Size:        416     646
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     778     812    1062 
   Size:        778      34     250

  Fragments arranged by size: 

                 778     250      34
 
BclI T'GATC_A

Cuts at:      0     606    1062 
   Size:        606     456
 
BglI GCCn_nnn'nGGC

Cuts at:      0     829    1062 
   Size:        829     233
 
Bme1580I G_kGCm'C

Cuts at:      0     311     939    1062 
   Size:        311     628     123

  Fragments arranged by size: 

                 628     311     123
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     155     767     933    1062 
   Size:        155     612     166     129

  Fragments arranged by size: 

                 612     166     155     129
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     832    1062 
   Size:        527     305     230

  Fragments arranged by size: 

                 527     305     230
 
BsaHI Gr'CG_yC

Cuts at:      0     392    1062 
   Size:        392     670
 
BsaWI w'CCGG_w

Cuts at:      0      90    1062 
   Size:         90     972
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     805    1062 
   Size:        805     257
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     311     636     939    1062 
   Size:        103     208     325     303     123

  Fragments arranged by size: 

                 325     303     208     123     103
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     467    1062 
   Size:        467     595
 
BsrFI r'CCGG_y

Cuts at:      0      90    1062 
   Size:         90     972
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     370    1062 
   Size:        370     692
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     347    1062 
   Size:        347     715
 
BtsI GCAGTG_nn'

Cuts at:      0     589    1062 
   Size:        589     473
 
DraI TTT'AAA

Cuts at:      0    1038    1062 
   Size:       1038      24
 
DraIII CAC_nnn'GTG

Cuts at:      0     921    1062 
   Size:        921     141
 
EaeI y'GGCC_r

Cuts at:      0     929    1062 
   Size:        929     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     832    1062 
   Size:        527     305     230

  Fragments arranged by size: 

                 527     305     230
 
EcoO109I rG'GnC_Cy

Cuts at:      0     935    1062 
   Size:        935     127
 
EcoRV GAT'ATC

Cuts at:      0     622    1062 
   Size:        622     440
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      39    1062 
   Size:         39    1023
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     412     444     611     643    1062 
   Size:        412      32     167      32     419

  Fragments arranged by size: 

                 419     412     167      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     878    1062 
   Size:        878     184
 
MscI TGG'CCA

Cuts at:      0     931    1062 
   Size:        931     131
 
MslI CAynn'nnrTG

Cuts at:      0     743    1062 
   Size:        743     319
 
MspA1I CmG'CkG

Cuts at:      0     386    1062 
   Size:        386     676
 
PshAI GACnn'nnGTC

Cuts at:      0     244    1062 
   Size:        244     818
 
PspOMI G'GGCC_C

Cuts at:      0     935    1062 
   Size:        935     127
 
SfcI C'TryA_G

Cuts at:      0     459    1062 
   Size:        459     603
 
SgrAI Cr'CCGG_yG

Cuts at:      0      90    1062 
   Size:         90     972
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     174    1062 
   Size:        174     888
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     946    1062 
   Size:        946     116
 
ZraI GAC'GTC

Cuts at:      0     393    1062 
   Size:        393     669
 

 Enzymes that do cut:

    AatII     AccI     AgeI     AhdI     AlfI     ApaI     ApoI     AvaI
    BanII     BbsI     BcgI     BclI     BglI Bme1580I     BmrI     BpmI
    BsaHI    BsaWI    BseRI Bsp1286I    BspMI    BsrFI   BstAPI    BtgZI
     BtsI     DraI   DraIII     EaeI  Eco57MI EcoO109I    EcoRV     FalI
    Hin4I     MmeI     MscI     MslI   MspA1I    PshAI   PspOMI     SfcI
    SgrAI    TaqII     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AleI
     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI    BciVI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI