Restriction map for
pF1KE2635
(Linear) (Six-base) MAPSORT of: KIEE2635.seq Check: 246 from: 1 to: 1170
KIEE2635.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 600 1170
Size: 251 349 570
Fragments arranged by size:
570 349 251
AflII C'TTAA_G
Cuts at: 0 691 1170
Size: 691 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1170
Size: 158 32 980
Fragments arranged by size:
980 158 32
ApoI r'AATT_y
Cuts at: 0 59 193 236 313 994 1170
Size: 59 134 43 77 681 176
Fragments arranged by size:
681 176 134 77 59 43
AvaI C'yCGr_G
Cuts at: 0 820 1170
Size: 820 350
BamHI G'GATC_C
Cuts at: 0 1092 1170
Size: 1092 78
BanI G'GyrC_C
Cuts at: 0 600 1170
Size: 600 570
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 1170
Size: 820 350
BmgBI CAC'GTC
Cuts at: 0 447 894 1170
Size: 447 447 276
Fragments arranged by size:
447 447 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1170
Size: 185 985
Bpu10I CC'TnA_GC
Cuts at: 0 515 805 1170
Size: 515 290 365
Fragments arranged by size:
515 365 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1118 1170
Size: 722 396 52
Fragments arranged by size:
722 396 52
BsaBI GATnn'nnATC
Cuts at: 0 1118 1170
Size: 1118 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 188 1170
Size: 158 30 982
Fragments arranged by size:
982 158 30
BspHI T'CATG_A
Cuts at: 0 524 1170
Size: 524 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 420 1170
Size: 420 750
BsrBI CCG'CTC
Cuts at: 0 825 1170
Size: 825 345
BsrDI GCAATG_nn'
Cuts at: 0 1083 1170
Size: 1083 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 664 1170
Size: 664 506
BstEII G'GTnAC_C
Cuts at: 0 145 1170
Size: 145 1025
BstYI r'GATC_y
Cuts at: 0 1092 1170
Size: 1092 78
BtgI C'CryG_G
Cuts at: 0 944 1170
Size: 944 226
BtsI GCAGTG_nn'
Cuts at: 0 662 1170
Size: 662 508
DraI TTT'AAA
Cuts at: 0 318 1170
Size: 318 852
EaeI y'GGCC_r
Cuts at: 0 947 1170
Size: 947 223
EarI CTCTTCn'nnn_
Cuts at: 0 397 1170
Size: 397 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1 670 1170
Size: 1 669 500
Fragments arranged by size:
669 500 1
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 251 600 1170
Size: 185 66 349 570
Fragments arranged by size:
570 349 185 66
EcoO109I rG'GnC_Cy
Cuts at: 0 439 519 1170
Size: 439 80 651
Fragments arranged by size:
651 439 80
EcoRI G'AATT_C
Cuts at: 0 59 1170
Size: 59 1111
HaeII r_GCGC'y
Cuts at: 0 490 1170
Size: 490 680
MscI TGG'CCA
Cuts at: 0 949 1170
Size: 949 221
MslI CAynn'nnrTG
Cuts at: 0 481 1026 1170
Size: 481 545 144
Fragments arranged by size:
545 481 144
NcoI C'CATG_G
Cuts at: 0 944 1170
Size: 944 226
PfoI T'CCnGG_A
Cuts at: 0 137 1170
Size: 137 1033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 1170
Size: 158 32 980
Fragments arranged by size:
980 158 32
PshAI GACnn'nnGTC
Cuts at: 0 885 1170
Size: 885 285
PspXI vC'TCGA_Gb
Cuts at: 0 820 1170
Size: 820 350
PstI C_TGCA'G
Cuts at: 0 351 1170
Size: 351 819
SfcI C'TryA_G
Cuts at: 0 347 590 1170
Size: 347 243 580
Fragments arranged by size:
580 347 243
SmlI C'TyrA_G
Cuts at: 0 691 737 820 1133 1170
Size: 691 46 83 313 37
Fragments arranged by size:
691 313 83 46 37
StyI C'CwwG_G
Cuts at: 0 935 944 1170
Size: 935 9 226
Fragments arranged by size:
935 226 9
SwaI ATTT'AAAT
Cuts at: 0 318 1170
Size: 318 852
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 164 1134 1170
Size: 164 970 36
Fragments arranged by size:
970 164 36
TatI w'GTAC_w
Cuts at: 0 256 453 1170
Size: 256 197 717
Fragments arranged by size:
717 256 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 316 348 1170
Size: 116 32 168 32 822
Fragments arranged by size:
822 168 116 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 942 984 1170
Size: 942 42 186
Fragments arranged by size:
942 186 42
XhoI C'TCGA_G
Cuts at: 0 820 1170
Size: 820 350
XmnI GAAnn'nnTTC
Cuts at: 0 610 1170
Size: 610 560
Enzymes that do cut:
AcuI AflII AloI ApoI AvaI BamHI BanI BbsI
BmgBI BpmI Bpu10I BpuEI BsaBI BsaXI BspHI BspMI
BsrBI BsrDI BstAPI BstEII BstYI BtgI BtsI DraI
EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII MscI
MslI NcoI PfoI PpiI PshAI PspXI PstI SfcI
SmlI StyI SwaI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpuMI PsiI PspOMI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI Tth111I
XbaI XmaI ZraI