Restriction map for
pF1KE2631
(Linear) (Six-base) MAPSORT of: KIEE2631.seq Check: 8117 from: 1 to: 3348
KIEE2631.orf
With 182 enzymes: SgfI *
July 8, 2016 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 3348
Size: 724 2624
AccI GT'mk_AC
Cuts at: 0 1334 3348
Size: 1334 2014
AclI AA'CG_TT
Cuts at: 0 2429 3348
Size: 2429 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1161 2113 3081 3348
Size: 1161 952 968 267
Fragments arranged by size:
1161 968 952 267
AfeI AGC'GCT
Cuts at: 0 2458 3348
Size: 2458 890
AflIII A'CryG_T
Cuts at: 0 1706 3348
Size: 1706 1642
AleI CACnn'nnGTG
Cuts at: 0 364 706 730 3157 3348
Size: 364 342 24 2427 191
Fragments arranged by size:
2427 364 342 191 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 724 2530 3348
Size: 724 1806 818
Fragments arranged by size:
1806 818 724
AlwNI CAG_nnn'CTG
Cuts at: 0 1740 2022 3348
Size: 1740 282 1326
Fragments arranged by size:
1740 1326 282
ApaI G_GGCC'C
Cuts at: 0 520 1518 1611 1991 3061 3348
Size: 520 998 93 380 1070 287
Fragments arranged by size:
1070 998 520 380 287 93
ApaLI G'TGCA_C
Cuts at: 0 2059 2494 3348
Size: 2059 435 854
Fragments arranged by size:
2059 854 435
AvaI C'yCGr_G
Cuts at: 0 48 1539 2287 3191 3271 3348
Size: 48 1491 748 904 80 77
Fragments arranged by size:
1491 904 748 80 77 48
BamHI G'GATC_C
Cuts at: 0 1468 3348
Size: 1468 1880
BanI G'GyrC_C
Cuts at: 0 292 532 592 700 1300 2137 2189 2336
Size: 292 240 60 108 600 837 52 147
Cuts at: 2336 2537 3348
Size: 201 811
Fragments arranged by size:
837 811 600 292 240 201 147 108
60 52
BanII G_rGCy'C
Cuts at: 0 520 1081 1518 1611 1991 2125 3061 3348
Size: 520 561 437 93 380 134 936 287
Fragments arranged by size:
936 561 520 437 380 287 134 93
BbeI G_GCGC'C
Cuts at: 0 2193 2340 3348
Size: 2193 147 1008
Fragments arranged by size:
2193 1008 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 765 1167 2719 3348
Size: 765 402 1552 629
Fragments arranged by size:
1552 765 629 402
BbvCI CC'TCA_GC
Cuts at: 0 1744 3348
Size: 1744 1604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1134 1168 2914 2948 3348
Size: 1134 34 1746 34 400
Fragments arranged by size:
1746 1134 400 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1925 3348
Size: 1925 1423
BfrBI ATG'CAT
Cuts at: 0 2679 2757 3348
Size: 2679 78 591
Fragments arranged by size:
2679 591 78
BglI GCCn_nnn'nGGC
Cuts at: 0 913 2170 3348
Size: 913 1257 1178
Fragments arranged by size:
1257 1178 913
Bme1580I G_kGCm'C
Cuts at: 0 295 520 562 595 1432 1518 1559 1611
Size: 295 225 42 33 837 86 41 52
Cuts at: 1611 1991 2063 2140 2498 3061 3348
Size: 380 72 77 358 563 287
Fragments arranged by size:
837 563 380 358 295 287 225 86
77 72 52 42 41 33
BmgBI CAC'GTC
Cuts at: 0 2360 3348
Size: 2360 988
BmrI ACTGGGnnnn_n'
Cuts at: 0 3056 3348
Size: 3056 292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2478 2527 3348
Size: 2478 49 821
Fragments arranged by size:
2478 821 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1095 1779 1999 2028 3348
Size: 1095 684 220 29 1320
Fragments arranged by size:
1320 1095 684 220 29
Bpu10I CC'TnA_GC
Cuts at: 0 1744 2453 3348
Size: 1744 709 895
Fragments arranged by size:
1744 895 709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2152 3348
Size: 2152 1196
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 3348
Size: 515 2833
BsaBI GATnn'nnATC
Cuts at: 0 1642 2569 3348
Size: 1642 927 779
Fragments arranged by size:
1642 927 779
BsaHI Gr'CG_yC
Cuts at: 0 338 2190 2337 3197 3348
Size: 338 1852 147 860 151
Fragments arranged by size:
1852 860 338 151 147
BsaWI w'CCGG_w
Cuts at: 0 2822 3000 3348
Size: 2822 178 348
Fragments arranged by size:
2822 348 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1327 1357 3348
Size: 1327 30 1991
Fragments arranged by size:
1991 1327 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 163 957 1125 2229 2917 2920 2986
Size: 160 3 794 168 1104 688 3 66
Cuts at: 2986 3063 3348
Size: 77 285
Fragments arranged by size:
1104 794 688 285 168 160 77 66
3 3
BseYI C'CCAG_C
Cuts at: 0 624 814 1492 2066 2087 2165 2938 2947
Size: 624 190 678 574 21 78 773 9
Cuts at: 2947 3348
Size: 401
Fragments arranged by size:
773 678 624 574 401 190 78 21
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1748 1753 2932 3348
Size: 295 1453 5 1179 416
Fragments arranged by size:
1453 1179 416 295 5
BsiEI CG_ry'CG
Cuts at: 0 3158 3324 3348
Size: 3158 166 24
Fragments arranged by size:
3158 166 24
BsiHKAI G_wGCw'C
Cuts at: 0 713 1081 2063 2498 2937 3290 3348
Size: 713 368 982 435 439 353 58
Fragments arranged by size:
982 713 439 435 368 353 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 925 2866 3348
Size: 925 1941 482
Fragments arranged by size:
1941 925 482
Bsp1286I G_dGCh'C
Cuts at: 0 295 520 562 595 713 1081 1432 1518
Size: 295 225 42 33 118 368 351 86
Cuts at: 1518 1559 1611 1991 2063 2125 2140 2498 2937
Size: 41 52 380 72 62 15 358 439
Cuts at: 2937 3061 3290 3348
Size: 124 229 58
Fragments arranged by size:
439 380 368 358 351 295 229 225
124 118 86 72 62 58 52 42
41 33 15
BspEI T'CCGG_A
Cuts at: 0 2822 3000 3348
Size: 2822 178 348
Fragments arranged by size:
2822 348 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 320 724 1557 3348
Size: 218 102 404 833 1791
Fragments arranged by size:
1791 833 404 218 102
BsrBI CCG'CTC
Cuts at: 0 1259 2775 2781 3348
Size: 1259 1516 6 567
Fragments arranged by size:
1516 1259 567 6
BsrDI GCAATG_nn'
Cuts at: 0 374 1649 1868 3249 3348
Size: 374 1275 219 1381 99
Fragments arranged by size:
1381 1275 374 219 99
BsrFI r'CCGG_y
Cuts at: 0 270 3348
Size: 270 3078
BsrGI T'GTAC_A
Cuts at: 0 581 3348
Size: 581 2767
BssSI C'ACGA_G
Cuts at: 0 1420 2810 3348
Size: 1420 1390 538
Fragments arranged by size:
1420 1390 538
BstAPI GCAn_nnn'nTGC
Cuts at: 0 652 1740 3348
Size: 652 1088 1608
Fragments arranged by size:
1608 1088 652
BstEII G'GTnAC_C
Cuts at: 0 868 936 2418 3348
Size: 868 68 1482 930
Fragments arranged by size:
1482 930 868 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 513 1451 3348
Size: 168 345 938 1897
Fragments arranged by size:
1897 938 345 168
BstYI r'GATC_y
Cuts at: 0 1025 1468 2619 2741 2786 2997 3348
Size: 1025 443 1151 122 45 211 351
Fragments arranged by size:
1151 1025 443 351 211 122 45
Bsu36I CC'TnA_GG
Cuts at: 0 1270 2978 3348
Size: 1270 1708 370
Fragments arranged by size:
1708 1270 370
BtgI C'CryG_G
Cuts at: 0 536 629 888 1450 1578 1902 3312 3348
Size: 536 93 259 562 128 324 1410 36
Fragments arranged by size:
1410 562 536 324 259 128 93 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 47 1299 1940 3348
Size: 47 1252 641 1408
Fragments arranged by size:
1408 1252 641 47
BtsI GCAGTG_nn'
Cuts at: 0 1736 2651 3348
Size: 1736 915 697
Fragments arranged by size:
1736 915 697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 237 272 3348
Size: 237 35 3076
Fragments arranged by size:
3076 237 35
EaeI y'GGCC_r
Cuts at: 0 194 437 632 722 803 1136 1310 1441
Size: 194 243 195 90 81 333 174 131
Cuts at: 1441 1575 2400 3155 3321 3348
Size: 134 825 755 166 27
Fragments arranged by size:
825 755 333 243 195 194 174 166
134 131 90 81 27
EagI C'GGCC_G
Cuts at: 0 3155 3321 3348
Size: 3155 166 27
Fragments arranged by size:
3155 166 27
EarI CTCTTCn'nnn_
Cuts at: 0 230 352 625 2171 2864 2885 3348
Size: 230 122 273 1546 693 21 463
Fragments arranged by size:
1546 693 463 273 230 122 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2568 2842 3348
Size: 2568 274 506
Fragments arranged by size:
2568 506 274
EcoICRI GAG'CTC
Cuts at: 0 1079 3348
Size: 1079 2269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1095 1161 1779 1999 2028 2113 3081 3348
Size: 1095 66 618 220 29 85 968 267
Fragments arranged by size:
1095 968 618 267 220 85 66 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 1090 1528 1663 2197 3348
Size: 214 876 438 135 534 1151
Fragments arranged by size:
1151 876 534 438 214 135
EcoO109I rG'GnC_Cy
Cuts at: 0 279 954 1266 1361 1514 1515 1608 1987
Size: 279 675 312 95 153 1 93 379
Cuts at: 1987 1988 2106 2283 3057 3058 3276 3348
Size: 1 118 177 774 1 218 72
Fragments arranged by size:
774 675 379 312 279 218 177 153
118 95 93 72 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 849 3111 3348
Size: 849 2262 237
Fragments arranged by size:
2262 849 237
HaeII r_GCGC'y
Cuts at: 0 2193 2340 2460 3348
Size: 2193 147 120 888
Fragments arranged by size:
2193 888 147 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1923 1955 2245 2277 2804 2817
Size: 1174 32 717 32 290 32 527 13
Cuts at: 2817 2836 2849 2910 2942 3348
Size: 19 13 61 32 406
Fragments arranged by size:
1174 717 527 406 290 61 32 32
32 32 19 13 13
HincII GTy'rAC
Cuts at: 0 756 3348
Size: 756 2592
HindIII A'AGCT_T
Cuts at: 0 3202 3348
Size: 3202 146
KasI G'GCGC_C
Cuts at: 0 2189 2336 3348
Size: 2189 147 1012
Fragments arranged by size:
2189 1012 147
MfeI C'AATT_G
Cuts at: 0 455 3348
Size: 455 2893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2267 3158 3348
Size: 2267 891 190
Fragments arranged by size:
2267 891 190
MscI TGG'CCA
Cuts at: 0 196 634 724 805 1312 1443 1577 2402
Size: 196 438 90 81 507 131 134 825
Cuts at: 2402 3348
Size: 946
Fragments arranged by size:
946 825 507 438 196 134 131 90
81
MslI CAynn'nnrTG
Cuts at: 0 298 304 364 445 706 718 730 948
Size: 298 6 60 81 261 12 12 218
Cuts at: 948 1169 1449 1711 2512 2608 3157 3348
Size: 221 280 262 801 96 549 191
Fragments arranged by size:
801 549 298 280 262 261 221 218
191 96 81 60 12 12 6
MspA1I CmG'CkG
Cuts at: 0 2019 2070 2091 2169 2398 2916 2942 3314
Size: 2019 51 21 78 229 518 26 372
Cuts at: 3314 3348
Size: 34
Fragments arranged by size:
2019 518 372 229 78 51 34 26
21
NarI GG'CG_CC
Cuts at: 0 2190 2337 3348
Size: 2190 147 1011
Fragments arranged by size:
2190 1011 147
NcoI C'CATG_G
Cuts at: 0 629 888 1450 1578 3348
Size: 629 259 562 128 1770
Fragments arranged by size:
1770 629 562 259 128
NotI GC'GGCC_GC
Cuts at: 0 3321 3348
Size: 3321 27
NsiI A_TGCA'T
Cuts at: 0 2681 2759 3348
Size: 2681 78 589
Fragments arranged by size:
2681 589 78
NspI r_CATG'y
Cuts at: 0 690 1710 2511 3150 3222 3348
Size: 690 1020 801 639 72 126
Fragments arranged by size:
1020 801 690 639 126 72
PasI CC'CwG_GG
Cuts at: 0 216 2965 3348
Size: 216 2749 383
Fragments arranged by size:
2749 383 216
PciI A'CATG_T
Cuts at: 0 1706 3348
Size: 1706 1642
PflMI CCAn_nnn'nTGG
Cuts at: 0 526 2006 2055 3348
Size: 526 1480 49 1293
Fragments arranged by size:
1480 1293 526 49
PfoI T'CCnGG_A
Cuts at: 0 2384 3348
Size: 2384 964
PpuMI rG'GwC_Cy
Cuts at: 0 1266 3276 3348
Size: 1266 2010 72
Fragments arranged by size:
2010 1266 72
PspOMI G'GGCC_C
Cuts at: 0 516 1514 1607 1987 3057 3348
Size: 516 998 93 380 1070 291
Fragments arranged by size:
1070 998 516 380 291 93
PstI C_TGCA'G
Cuts at: 0 326 1502 1532 1568 2300 3348
Size: 326 1176 30 36 732 1048
Fragments arranged by size:
1176 1048 732 326 36 30
PvuII CAG'CTG
Cuts at: 0 2019 2070 2091 2398 2916 2942 3348
Size: 2019 51 21 307 518 26 406
Fragments arranged by size:
2019 518 406 307 51 26 21
SacI G_AGCT'C
Cuts at: 0 1081 3348
Size: 1081 2267
SacII CC_GC'GG
Cuts at: 0 3315 3348
Size: 3315 33
SbfI CC_TGCA'GG
Cuts at: 0 1568 3348
Size: 1568 1780
SfcI C'TryA_G
Cuts at: 0 322 1498 1528 1564 2296 3348
Size: 322 1176 30 36 732 1052
Fragments arranged by size:
1176 1052 732 322 36 30
SfoI GGC'GCC
Cuts at: 0 2191 2338 3348
Size: 2191 147 1010
Fragments arranged by size:
2191 1010 147
SmaI CCC'GGG
Cuts at: 0 50 3193 3348
Size: 50 3143 155
Fragments arranged by size:
3143 155 50
SmlI C'TyrA_G
Cuts at: 0 1539 2131 3271 3348
Size: 1539 592 1140 77
Fragments arranged by size:
1539 1140 592 77
SphI G_CATG'C
Cuts at: 0 2511 3150 3348
Size: 2511 639 198
Fragments arranged by size:
2511 639 198
StyI C'CwwG_G
Cuts at: 0 65 629 888 1091 1450 1578 3026 3348
Size: 65 564 259 203 359 128 1448 322
Fragments arranged by size:
1448 564 359 322 259 203 128 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 730 858 1767 3348
Size: 354 376 128 909 1581
Fragments arranged by size:
1581 909 376 354 128
TatI w'GTAC_w
Cuts at: 0 581 1889 3348
Size: 581 1308 1459
Fragments arranged by size:
1459 1308 581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 833 865 3348
Size: 833 32 2483
Fragments arranged by size:
2483 833 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 1036 3348
Size: 585 451 2312
Fragments arranged by size:
2312 585 451
XhoI C'TCGA_G
Cuts at: 0 1539 3271 3348
Size: 1539 1732 77
Fragments arranged by size:
1732 1539 77
XmaI C'CCGG_G
Cuts at: 0 48 3191 3348
Size: 48 3143 157
Fragments arranged by size:
3143 157 48
XmnI GAAnn'nnTTC
Cuts at: 0 2218 3348
Size: 2218 1130
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NotI NsiI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SbfI SfcI SfoI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BclI BglII BlpI BmtI
BsaAI BsiWI BsmI BspHI BssHII BstBI BstZ17I ClaI
DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI
HpaI KpnI MluI NaeI NdeI NgoMIV NheI NruI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI ZraI