Restriction map for
pF1KE2615
(Linear) (Six-base) MAPSORT of: KIEE2615.seq Check: 9271 from: 1 to: 1062
KIEE2615.orf
With 182 enzymes: SgfI *
April 1, 2016 14:38 ..
AccI GT'mk_AC
Cuts at: 0 1009 1062
Size: 1009 53
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 856 1009 1062
Size: 68 788 153 53
Fragments arranged by size:
788 153 68 53
AfeI AGC'GCT
Cuts at: 0 424 892 1062
Size: 424 468 170
Fragments arranged by size:
468 424 170
AflIII A'CryG_T
Cuts at: 0 590 1062
Size: 590 472
AlwNI CAG_nnn'CTG
Cuts at: 0 88 605 1062
Size: 88 517 457
Fragments arranged by size:
517 457 88
ApoI r'AATT_y
Cuts at: 0 480 1062
Size: 480 582
BanI G'GyrC_C
Cuts at: 0 343 534 1062
Size: 343 191 528
Fragments arranged by size:
528 343 191
BanII G_rGCy'C
Cuts at: 0 975 1062
Size: 975 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 662 1062
Size: 56 606 400
Fragments arranged by size:
606 400 56
BbvCI CC'TCA_GC
Cuts at: 0 455 1062
Size: 455 607
Bme1580I G_kGCm'C
Cuts at: 0 539 1062
Size: 539 523
BmrI ACTGGGnnnn_n'
Cuts at: 0 685 1062
Size: 685 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 576 989 1062
Size: 444 132 413 73
Fragments arranged by size:
444 413 132 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 963 1062
Size: 645 318 99
Fragments arranged by size:
645 318 99
Bpu10I CC'TnA_GC
Cuts at: 0 455 1062
Size: 455 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1062
Size: 916 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 716 1062
Size: 716 346
BsaAI yAC'GTr
Cuts at: 0 257 1062
Size: 257 805
BsaWI w'CCGG_w
Cuts at: 0 623 1062
Size: 623 439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 1062
Size: 805 30 227
Fragments arranged by size:
805 227 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 443 813 1062
Size: 443 370 249
Fragments arranged by size:
443 370 249
BseYI C'CCAG_C
Cuts at: 0 830 946 1062
Size: 830 116 116
Fragments arranged by size:
830 116 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 122 331 1062
Size: 92 30 209 731
Fragments arranged by size:
731 209 92 30
BsiHKAI G_wGCw'C
Cuts at: 0 793 804 1062
Size: 793 11 258
Fragments arranged by size:
793 258 11
BsmI GAATG_Cn'
Cuts at: 0 83 225 1062
Size: 83 142 837
Fragments arranged by size:
837 142 83
Bsp1286I G_dGCh'C
Cuts at: 0 539 793 804 975 1062
Size: 539 254 11 171 87
Fragments arranged by size:
539 254 171 87 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 310 1062
Size: 310 752
BsrFI r'CCGG_y
Cuts at: 0 126 725 1062
Size: 126 599 337
Fragments arranged by size:
599 337 126
BssSI C'ACGA_G
Cuts at: 0 772 1062
Size: 772 290
BstYI r'GATC_y
Cuts at: 0 875 1062
Size: 875 187
Bsu36I CC'TnA_GG
Cuts at: 0 551 1062
Size: 551 511
BtgI C'CryG_G
Cuts at: 0 359 879 1062
Size: 359 520 183
Fragments arranged by size:
520 359 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 452 1062
Size: 452 610
BtsI GCAGTG_nn'
Cuts at: 0 802 1062
Size: 802 260
DrdI GACnn_nn'nnGTC
Cuts at: 0 240 1062
Size: 240 822
EaeI y'GGCC_r
Cuts at: 0 293 362 1062
Size: 293 69 700
Fragments arranged by size:
700 293 69
EarI CTCTTCn'nnn_
Cuts at: 0 734 1062
Size: 734 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 645 856 963 1009 1062
Size: 68 577 211 107 46 53
Fragments arranged by size:
577 211 107 68 53 46
EcoO109I rG'GnC_Cy
Cuts at: 0 1048 1062
Size: 1048 14
HaeII r_GCGC'y
Cuts at: 0 408 426 894 1062
Size: 408 18 468 168
Fragments arranged by size:
468 408 168 18
MscI TGG'CCA
Cuts at: 0 295 364 1062
Size: 295 69 698
Fragments arranged by size:
698 295 69
MspA1I CmG'CkG
Cuts at: 0 315 1062
Size: 315 747
NcoI C'CATG_G
Cuts at: 0 359 1062
Size: 359 703
NspI r_CATG'y
Cuts at: 0 594 1062
Size: 594 468
PasI CC'CwG_GG
Cuts at: 0 784 1062
Size: 784 278
PciI A'CATG_T
Cuts at: 0 590 1062
Size: 590 472
PflMI CCAn_nnn'nTGG
Cuts at: 0 853 1062
Size: 853 209
PshAI GACnn'nnGTC
Cuts at: 0 56 1062
Size: 56 1006
PvuII CAG'CTG
Cuts at: 0 315 1062
Size: 315 747
SgrAI Cr'CCGG_yG
Cuts at: 0 126 1062
Size: 126 936
SmlI C'TyrA_G
Cuts at: 0 931 1062
Size: 931 131
SspI AAT'ATT
Cuts at: 0 862 1062
Size: 862 200
StuI AGG'CCT
Cuts at: 0 922 1062
Size: 922 140
StyI C'CwwG_G
Cuts at: 0 359 923 1062
Size: 359 564 139
Fragments arranged by size:
564 359 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1062
Size: 976 32 54
Fragments arranged by size:
976 54 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 149 648 1062
Size: 149 499 414
Fragments arranged by size:
499 414 149
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApoI BanI BanII
BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssSI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EarI Eco57MI EcoO109I HaeII
MscI MspA1I NcoI NspI PasI PciI PflMI PshAI
PvuII SgrAI SmlI SspI StuI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BsaBI BsaHI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI XmnI ZraI