Restriction map for
pF1KE2611
(Linear) (Six-base) MAPSORT of: KIEE2611.seq Check: 5329 from: 1 to: 885
KIEE2611.orf
With 182 enzymes: SgfI *
February 29, 2016 12:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 885
Size: 693 192
AlwNI CAG_nnn'CTG
Cuts at: 0 45 635 817 885
Size: 45 590 182 68
Fragments arranged by size:
590 182 68 45
ApaI G_GGCC'C
Cuts at: 0 751 885
Size: 751 134
AvaI C'yCGr_G
Cuts at: 0 653 885
Size: 653 232
BanII G_rGCy'C
Cuts at: 0 324 561 578 751 885
Size: 324 237 17 173 134
Fragments arranged by size:
324 237 173 134 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 699 885
Size: 699 186
BlpI GC'TnA_GC
Cuts at: 0 63 885
Size: 63 822
Bme1580I G_kGCm'C
Cuts at: 0 97 751 885
Size: 97 654 134
Fragments arranged by size:
654 134 97
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 885
Size: 318 567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 837 885
Size: 837 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 885
Size: 528 357
BsaI GGTCTCn'nnnn_
Cuts at: 0 650 885
Size: 650 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 326 356 885
Size: 326 30 529
Fragments arranged by size:
529 326 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 885
Size: 505 380
BseYI C'CCAG_C
Cuts at: 0 252 453 648 692 885
Size: 252 201 195 44 193
Fragments arranged by size:
252 201 195 193 44
Bsp1286I G_dGCh'C
Cuts at: 0 97 324 561 578 751 885
Size: 97 227 237 17 173 134
Fragments arranged by size:
237 227 173 134 97 17
BsrDI GCAATG_nn'
Cuts at: 0 212 275 578 605 885
Size: 212 63 303 27 280
Fragments arranged by size:
303 280 212 63 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 552 885
Size: 219 333 333
Fragments arranged by size:
333 333 219
Bsu36I CC'TnA_GG
Cuts at: 0 8 885
Size: 8 877
BtsI GCAGTG_nn'
Cuts at: 0 677 885
Size: 677 208
EaeI y'GGCC_r
Cuts at: 0 209 500 885
Size: 209 291 385
Fragments arranged by size:
385 291 209
EarI CTCTTCn'nnn_
Cuts at: 0 49 885
Size: 49 836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 693 885
Size: 528 165 192
Fragments arranged by size:
528 192 165
EcoO109I rG'GnC_Cy
Cuts at: 0 748 885
Size: 748 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 78 885
Size: 78 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 155 187 764 796 885
Size: 155 32 577 32 89
Fragments arranged by size:
577 155 89 32 32
HindIII A'AGCT_T
Cuts at: 0 30 885
Size: 30 855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 885
Size: 165 720
MscI TGG'CCA
Cuts at: 0 211 502 885
Size: 211 291 383
Fragments arranged by size:
383 291 211
MslI CAynn'nnrTG
Cuts at: 0 217 885
Size: 217 668
MspA1I CmG'CkG
Cuts at: 0 256 885
Size: 256 629
PasI CC'CwG_GG
Cuts at: 0 618 885
Size: 618 267
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 885
Size: 330 555
PshAI GACnn'nnGTC
Cuts at: 0 384 662 885
Size: 384 278 223
Fragments arranged by size:
384 278 223
PspOMI G'GGCC_C
Cuts at: 0 747 885
Size: 747 138
PvuII CAG'CTG
Cuts at: 0 256 885
Size: 256 629
SapI GCTCTTCn'nnn_
Cuts at: 0 49 885
Size: 49 836
SfcI C'TryA_G
Cuts at: 0 740 885
Size: 740 145
SmlI C'TyrA_G
Cuts at: 0 653 885
Size: 653 232
StuI AGG'CCT
Cuts at: 0 569 776 885
Size: 569 207 109
Fragments arranged by size:
569 207 109
StyI C'CwwG_G
Cuts at: 0 114 683 885
Size: 114 569 202
Fragments arranged by size:
569 202 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 326 358 885
Size: 326 32 527
Fragments arranged by size:
527 326 32
Tth111I GACn'n_nGTC
Cuts at: 0 823 885
Size: 823 62
XhoI C'TCGA_G
Cuts at: 0 653 885
Size: 653 232
XmnI GAAnn'nnTTC
Cuts at: 0 295 885
Size: 295 590
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanII BbsI BlpI Bme1580I
BmrI BplI BpmI BsaI BsaXI BseRI BseYI Bsp1286I
BsrDI BstXI Bsu36I BtsI EaeI EarI Eco57MI EcoO109I
FalI Hin4I HindIII MmeI MscI MslI MspA1I PasI
PflMI PshAI PspOMI PvuII SapI SfcI SmlI StuI
StyI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI XbaI XcmI XmaI ZraI