(Linear) (Six-base) MAPSORT of: KIEE2604.seq  Check: 3865  from: 1  to: 537



KIEE2604.orf

 With 182 enzymes: SgfI * 

                        January 19, 2016 12:01  ..

AleI CACnn'nnGTG

Cuts at:      0     413     537 
   Size:        413     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     279     537 
   Size:        279     258
 
ApoI r'AATT_y

Cuts at:      0     286     537 
   Size:        286     251
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      85     537 
   Size:         85     452
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52     537 
   Size:         52     485
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     419     537 
   Size:        419     118
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     431     537 
   Size:        331     100     106

  Fragments arranged by size: 

                 331     106     100
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     307     537 
   Size:        307     230
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     489     537 
   Size:        489      48
 
BsiEI CG_ry'CG

Cuts at:      0      52     537 
   Size:         52     485
 
BsrFI r'CCGG_y

Cuts at:      0      51     537 
   Size:         51     486
 
BssSI C'ACGA_G

Cuts at:      0     413     537 
   Size:        413     124
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     196     537 
   Size:        196     341
 
BtgI C'CryG_G

Cuts at:      0     189     195     537 
   Size:        189       6     342

  Fragments arranged by size: 

                 342     189       6
 
EaeI y'GGCC_r

Cuts at:      0     192     537 
   Size:        192     345
 
EarI CTCTTCn'nnn_

Cuts at:      0     214     537 
   Size:        214     323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     431     537 
   Size:        331     100     106

  Fragments arranged by size: 

                 331     106     100
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     393     537 
   Size:        393     144
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     537 
   Size:        120     417
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     253     285     537 
   Size:        253      32     252

  Fragments arranged by size: 

                 253     252      32
 
MfeI C'AATT_G

Cuts at:      0     257     537 
   Size:        257     280
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     537 
   Size:         69     468
 
MscI TGG'CCA

Cuts at:      0     194     537 
   Size:        194     343
 
MslI CAynn'nnrTG

Cuts at:      0     194     413     537 
   Size:        194     219     124

  Fragments arranged by size: 

                 219     194     124
 
MspA1I CmG'CkG

Cuts at:      0     442     537 
   Size:        442      95
 
NcoI C'CATG_G

Cuts at:      0     189     195     537 
   Size:        189       6     342

  Fragments arranged by size: 

                 342     189       6
 
PfoI T'CCnGG_A

Cuts at:      0     155     537 
   Size:        155     382
 
PshAI GACnn'nnGTC

Cuts at:      0     319     537 
   Size:        319     218
 
PstI C_TGCA'G

Cuts at:      0     426     537 
   Size:        426     111
 
SfcI C'TryA_G

Cuts at:      0     422     537 
   Size:        422     115
 
StyI C'CwwG_G

Cuts at:      0     189     195     537 
   Size:        189       6     342

  Fragments arranged by size: 

                 342     189       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278     480     524     537 
   Size:        278     202      44      13

  Fragments arranged by size: 

                 278     202      44      13
 
TatI w'GTAC_w

Cuts at:      0     108     537 
   Size:        108     429
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     137     169     537 
   Size:        137      32     368

  Fragments arranged by size: 

                 368     137      32
 

 Enzymes that do cut:

     AleI    AlwNI     ApoI    BciVI     BglI     BplI     BpmI     BsaI
    BseRI    BsiEI    BsrFI    BssSI    BstXI     BtgI     EaeI     EarI
  Eco57MI    EcoNI     FalI    Hin4I     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     PfoI    PshAI     PstI     SfcI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI