Restriction map for
pF1KE2602
(Linear) (Six-base) MAPSORT of: KIEE2602.seq Check: 9436 from: 1 to: 2226
KIEE2602.orf
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 82 2226
Size: 82 2144
AccI GT'mk_AC
Cuts at: 0 1040 2226
Size: 1040 1186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 395 515 551 997 1426 1670 2226
Size: 347 48 120 36 446 429 244 556
Fragments arranged by size:
556 446 429 347 244 120 48 36
AleI CACnn'nnGTG
Cuts at: 0 307 1832 2226
Size: 307 1525 394
Fragments arranged by size:
1525 394 307
AlwNI CAG_nnn'CTG
Cuts at: 0 629 694 1346 1768 2226
Size: 629 65 652 422 458
Fragments arranged by size:
652 629 458 422 65
ApoI r'AATT_y
Cuts at: 0 1937 2226
Size: 1937 289
BamHI G'GATC_C
Cuts at: 0 269 2226
Size: 269 1957
BanI G'GyrC_C
Cuts at: 0 454 1051 2226
Size: 454 597 1175
Fragments arranged by size:
1175 597 454
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 626 794 1259 2226
Size: 503 123 168 465 967
Fragments arranged by size:
967 503 465 168 123
BbvCI CC'TCA_GC
Cuts at: 0 1287 1731 2226
Size: 1287 444 495
Fragments arranged by size:
1287 495 444
BclI T'GATC_A
Cuts at: 0 1952 2048 2226
Size: 1952 96 178
Fragments arranged by size:
1952 178 96
BlpI GC'TnA_GC
Cuts at: 0 49 2226
Size: 49 2177
BmgBI CAC'GTC
Cuts at: 0 531 2226
Size: 531 1695
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 969 1717 1751 1826 2226
Size: 330 639 748 34 75 400
Fragments arranged by size:
748 639 400 330 75 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1544 2226
Size: 1544 682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 897 1407 2144 2226
Size: 531 366 510 737 82
Fragments arranged by size:
737 531 510 366 82
Bpu10I CC'TnA_GC
Cuts at: 0 1287 1731 2226
Size: 1287 444 495
Fragments arranged by size:
1287 495 444
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 1123 1628 1805 2226
Size: 217 906 505 177 421
Fragments arranged by size:
906 505 421 217 177
BsaAI yAC'GTr
Cuts at: 0 105 2226
Size: 105 2121
BsaBI GATnn'nnATC
Cuts at: 0 1863 2226
Size: 1863 363
BsaWI w'CCGG_w
Cuts at: 0 999 2226
Size: 999 1227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 2226
Size: 265 30 1931
Fragments arranged by size:
1931 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 534 2226
Size: 534 1692
BseYI C'CCAG_C
Cuts at: 0 508 824 2226
Size: 508 316 1402
Fragments arranged by size:
1402 508 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 2226
Size: 311 1915
BsiHKAI G_wGCw'C
Cuts at: 0 679 2226
Size: 679 1547
Bsp1286I G_dGCh'C
Cuts at: 0 679 2226
Size: 679 1547
BspEI T'CCGG_A
Cuts at: 0 999 2226
Size: 999 1227
BspHI T'CATG_A
Cuts at: 0 1116 2226
Size: 1116 1110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 88 221 235 410 2204 2226
Size: 82 6 133 14 175 1794 22
Fragments arranged by size:
1794 175 133 82 22 14 6
BsrDI GCAATG_nn'
Cuts at: 0 2000 2067 2226
Size: 2000 67 159
Fragments arranged by size:
2000 159 67
BsrGI T'GTAC_A
Cuts at: 0 311 385 600 2226
Size: 311 74 215 1626
Fragments arranged by size:
1626 311 215 74
BssSI C'ACGA_G
Cuts at: 0 40 2226
Size: 40 2186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1834 2226
Size: 1834 392
BstYI r'GATC_y
Cuts at: 0 269 1858 2226
Size: 269 1589 368
Fragments arranged by size:
1589 368 269
BtgI C'CryG_G
Cuts at: 0 934 1379 2226
Size: 934 445 847
Fragments arranged by size:
934 847 445
BtsI GCAGTG_nn'
Cuts at: 0 1055 2226
Size: 1055 1171
ClaI AT'CG_AT
Cuts at: 0 65 2226
Size: 65 2161
EarI CTCTTCn'nnn_
Cuts at: 0 1121 1626 2226
Size: 1121 505 600
Fragments arranged by size:
1121 600 505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 395 515 531 551 897 997 1407
Size: 347 48 120 16 20 346 100 410
Cuts at: 1407 1426 1670 2144 2226
Size: 19 244 474 82
Fragments arranged by size:
474 410 347 346 244 120 100 82
48 20 19 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 2226
Size: 157 2069
EcoO109I rG'GnC_Cy
Cuts at: 0 1495 1712 1745 1841 2226
Size: 1495 217 33 96 385
Fragments arranged by size:
1495 385 217 96 33
EcoRV GAT'ATC
Cuts at: 0 1234 2226
Size: 1234 992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1027 2226
Size: 1027 1199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 373 405 563 595 895 927
Size: 23 32 318 32 158 32 300 32
Cuts at: 927 1405 1437 1482 1514 1768 1800 1861 1893
Size: 478 32 45 32 254 32 61 32
Cuts at: 1893 2226
Size: 333
Fragments arranged by size:
478 333 318 300 254 158 61 45
32 32 32 32 32 32 32 32
23
HincII GTy'rAC
Cuts at: 0 444 2010 2226
Size: 444 1566 216
Fragments arranged by size:
1566 444 216
HpaI GTT'AAC
Cuts at: 0 444 2226
Size: 444 1782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1603 1752 1805 2226
Size: 350 1253 149 53 421
Fragments arranged by size:
1253 421 350 149 53
MslI CAynn'nnrTG
Cuts at: 0 307 670 1027 1723 1832 1880 2226
Size: 307 363 357 696 109 48 346
Fragments arranged by size:
696 363 357 346 307 109 48
MspA1I CmG'CkG
Cuts at: 0 48 148 539 1279 2179 2226
Size: 48 100 391 740 900 47
Fragments arranged by size:
900 740 391 100 48 47
NcoI C'CATG_G
Cuts at: 0 1379 2226
Size: 1379 847
NspI r_CATG'y
Cuts at: 0 256 2226
Size: 256 1970
PfoI T'CCnGG_A
Cuts at: 0 1345 2226
Size: 1345 881
PmlI CAC'GTG
Cuts at: 0 105 2226
Size: 105 2121
PpuMI rG'GwC_Cy
Cuts at: 0 1495 1712 1745 1841 2226
Size: 1495 217 33 96 385
Fragments arranged by size:
1495 385 217 96 33
PshAI GACnn'nnGTC
Cuts at: 0 1424 2226
Size: 1424 802
PstI C_TGCA'G
Cuts at: 0 232 1284 1764 2201 2226
Size: 232 1052 480 437 25
Fragments arranged by size:
1052 480 437 232 25
PvuII CAG'CTG
Cuts at: 0 1279 2179 2226
Size: 1279 900 47
Fragments arranged by size:
1279 900 47
SanDI GG'GwC_CC
Cuts at: 0 1841 2226
Size: 1841 385
SbfI CC_TGCA'GG
Cuts at: 0 232 2226
Size: 232 1994
SfcI C'TryA_G
Cuts at: 0 123 228 686 1280 1760 2197 2226
Size: 123 105 458 594 480 437 29
Fragments arranged by size:
594 480 458 437 123 105 29
SpeI A'CTAG_T
Cuts at: 0 679 2226
Size: 679 1547
StyI C'CwwG_G
Cuts at: 0 1379 1976 2226
Size: 1379 597 250
Fragments arranged by size:
1379 597 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1260 2226
Size: 1260 966
TatI w'GTAC_w
Cuts at: 0 311 385 600 1171 1432 2226
Size: 311 74 215 571 261 794
Fragments arranged by size:
794 571 311 261 215 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 2226
Size: 265 32 1929
Fragments arranged by size:
1929 265 32
Tth111I GACn'n_nGTC
Cuts at: 0 1388 1743 2226
Size: 1388 355 483
Fragments arranged by size:
1388 483 355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1852 2226
Size: 1852 374
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApoI BamHI BanI
BbsI BbvCI BclI BlpI BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI
BssSI BstXI BstYI BtgI BtsI ClaI EarI Eco57MI
EcoNI EcoO109I EcoRV FalI Hin4I HincII HpaI MmeI
MslI MspA1I NcoI NspI PfoI PmlI PpuMI PshAI
PstI PvuII SanDI SbfI SfcI SpeI StyI TaqII
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BcgI BciVI BfrBI BglI
BglII Bme1580I BmtI BpuEI BsaHI BsiEI BsiWI BsmI
BsmBI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
SspI StuI SwaI XbaI XhoI XmaI XmnI ZraI