Restriction map for
pF1KE2601
(Linear) (Six-base) MAPSORT of: KIEE2601.seq Check: 4071 from: 1 to: 2184
KIEE2601.orf
With 182 enzymes: SgfI *
January 19, 2016 12:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 655 1852 2184
Size: 655 1197 332
Fragments arranged by size:
1197 655 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1408 2184
Size: 121 1287 776
Fragments arranged by size:
1287 776 121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1999 2184
Size: 1999 185
AlwNI CAG_nnn'CTG
Cuts at: 0 72 2184
Size: 72 2112
ApaI G_GGCC'C
Cuts at: 0 408 2184
Size: 408 1776
ApaLI G'TGCA_C
Cuts at: 0 2128 2184
Size: 2128 56
ApoI r'AATT_y
Cuts at: 0 194 451 784 1029 1504 1635 1942 2057
Size: 194 257 333 245 475 131 307 115
Cuts at: 2057 2184
Size: 127
Fragments arranged by size:
475 333 307 257 245 194 131 127
115
AvaI C'yCGr_G
Cuts at: 0 1853 2184
Size: 1853 331
BanII G_rGCy'C
Cuts at: 0 408 1682 2184
Size: 408 1274 502
Fragments arranged by size:
1274 502 408
BbvCI CC'TCA_GC
Cuts at: 0 660 1205 2184
Size: 660 545 979
Fragments arranged by size:
979 660 545
BciVI GTATCCnnnnn_n'
Cuts at: 0 1532 2184
Size: 1532 652
BclI T'GATC_A
Cuts at: 0 650 2184
Size: 650 1534
BfrBI ATG'CAT
Cuts at: 0 1088 2184
Size: 1088 1096
BlpI GC'TnA_GC
Cuts at: 0 79 2184
Size: 79 2105
Bme1580I G_kGCm'C
Cuts at: 0 408 2132 2184
Size: 408 1724 52
Fragments arranged by size:
1724 408 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 1683 2184
Size: 1683 501
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1268 2184
Size: 1268 916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 2051 2162 2184
Size: 1045 1006 111 22
Fragments arranged by size:
1045 1006 111 22
Bpu10I CC'TnA_GC
Cuts at: 0 660 1130 1205 2184
Size: 660 470 75 979
Fragments arranged by size:
979 660 470 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 2184
Size: 722 1462
BsaI GGTCTCn'nnnn_
Cuts at: 0 703 2184
Size: 703 1481
BsaAI yAC'GTr
Cuts at: 0 1784 2184
Size: 1784 400
BsaBI GATnn'nnATC
Cuts at: 0 732 1520 2184
Size: 732 788 664
Fragments arranged by size:
788 732 664
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1994 2024 2054 2135 2165 2184
Size: 1994 30 30 81 30 19
Fragments arranged by size:
1994 81 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 227 2184
Size: 227 1957
BseYI C'CCAG_C
Cuts at: 0 339 994 1178 2101 2184
Size: 339 655 184 923 83
Fragments arranged by size:
923 655 339 184 83
BsiHKAI G_wGCw'C
Cuts at: 0 871 1620 1682 2132 2184
Size: 871 749 62 450 52
Fragments arranged by size:
871 749 450 62 52
BsmI GAATG_Cn'
Cuts at: 0 846 1090 1603 1649 1877 2184
Size: 846 244 513 46 228 307
Fragments arranged by size:
846 513 307 244 228 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2022 2027 2184
Size: 2022 5 157
Fragments arranged by size:
2022 157 5
Bsp1286I G_dGCh'C
Cuts at: 0 408 871 1620 1682 2132 2184
Size: 408 463 749 62 450 52
Fragments arranged by size:
749 463 450 408 62 52
BspHI T'CATG_A
Cuts at: 0 647 2184
Size: 647 1537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 655 1852 2184
Size: 655 1197 332
Fragments arranged by size:
1197 655 332
BsrDI GCAATG_nn'
Cuts at: 0 689 2184
Size: 689 1495
BsrGI T'GTAC_A
Cuts at: 0 414 1898 2184
Size: 414 1484 286
Fragments arranged by size:
1484 414 286
BssSI C'ACGA_G
Cuts at: 0 2170 2184
Size: 2170 14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 2184
Size: 75 2109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 2184
Size: 465 1719
BstYI r'GATC_y
Cuts at: 0 2163 2184
Size: 2163 21
BtgI C'CryG_G
Cuts at: 0 518 1001 2184
Size: 518 483 1183
Fragments arranged by size:
1183 518 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 776 958 2184
Size: 776 182 1226
Fragments arranged by size:
1226 776 182
BtsI GCAGTG_nn'
Cuts at: 0 1983 2184
Size: 1983 201
EaeI y'GGCC_r
Cuts at: 0 58 2012 2184
Size: 58 1954 172
Fragments arranged by size:
1954 172 58
EarI CTCTTCn'nnn_
Cuts at: 0 701 933 1384 2184
Size: 701 232 451 800
Fragments arranged by size:
800 701 451 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 2184
Size: 331 1853
EcoICRI GAG'CTC
Cuts at: 0 1680 2184
Size: 1680 504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1045 1408 2051 2162 2184
Size: 121 924 363 643 111 22
Fragments arranged by size:
924 643 363 121 111 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1108 2184
Size: 1108 1076
EcoO109I rG'GnC_Cy
Cuts at: 0 404 1209 2184
Size: 404 805 975
Fragments arranged by size:
975 805 404
FspI TGC'GCA
Cuts at: 0 688 2184
Size: 688 1496
FspAI rTGC'GCAy
Cuts at: 0 688 2184
Size: 688 1496
HaeII r_GCGC'y
Cuts at: 0 91 2184
Size: 91 2093
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 870 902 2184
Size: 870 32 1282
Fragments arranged by size:
1282 870 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1329 2133 2184
Size: 1329 804 51
Fragments arranged by size:
1329 804 51
MslI CAynn'nnrTG
Cuts at: 0 281 839 2080 2184
Size: 281 558 1241 104
Fragments arranged by size:
1241 558 281 104
MspA1I CmG'CkG
Cuts at: 0 30 72 2101 2184
Size: 30 42 2029 83
Fragments arranged by size:
2029 83 42 30
NcoI C'CATG_G
Cuts at: 0 518 2184
Size: 518 1666
NdeI CA'TA_TG
Cuts at: 0 1362 2184
Size: 1362 822
NsiI A_TGCA'T
Cuts at: 0 1090 2184
Size: 1090 1094
NspI r_CATG'y
Cuts at: 0 109 227 508 687 1548 2184
Size: 109 118 281 179 861 636
Fragments arranged by size:
861 636 281 179 118 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 427 1240 1575 1784 2184
Size: 427 813 335 209 400
Fragments arranged by size:
813 427 400 335 209
PfoI T'CCnGG_A
Cuts at: 0 1109 2184
Size: 1109 1075
PmlI CAC'GTG
Cuts at: 0 1784 2184
Size: 1784 400
PpuMI rG'GwC_Cy
Cuts at: 0 1209 2184
Size: 1209 975
PspOMI G'GGCC_C
Cuts at: 0 404 2184
Size: 404 1780
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 138 170 2184
Size: 138 32 2014
Fragments arranged by size:
2014 138 32
PvuII CAG'CTG
Cuts at: 0 72 2101 2184
Size: 72 2029 83
Fragments arranged by size:
2029 83 72
SacI G_AGCT'C
Cuts at: 0 1682 2184
Size: 1682 502
SapI GCTCTTCn'nnn_
Cuts at: 0 933 2184
Size: 933 1251
SexAI A'CCwGG_T
Cuts at: 0 1278 2184
Size: 1278 906
SfcI C'TryA_G
Cuts at: 0 959 2184
Size: 959 1225
SmlI C'TyrA_G
Cuts at: 0 737 1853 2184
Size: 737 1116 331
Fragments arranged by size:
1116 737 331
SphI G_CATG'C
Cuts at: 0 687 2184
Size: 687 1497
SspI AAT'ATT
Cuts at: 0 985 1443 2184
Size: 985 458 741
Fragments arranged by size:
985 741 458
StuI AGG'CCT
Cuts at: 0 658 2184
Size: 658 1526
StyI C'CwwG_G
Cuts at: 0 518 2184
Size: 518 1666
TatI w'GTAC_w
Cuts at: 0 113 414 1898 2184
Size: 113 301 1484 286
Fragments arranged by size:
1484 301 286 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1994 2026 2184
Size: 1994 32 158
Fragments arranged by size:
1994 158 32
Tth111I GACn'n_nGTC
Cuts at: 0 1105 2184
Size: 1105 1079
XhoI C'TCGA_G
Cuts at: 0 1853 2184
Size: 1853 331
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI
BanII BbvCI BciVI BclI BfrBI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I MmeI MslI MspA1I NcoI
NdeI NsiI NspI PflMI PfoI PmlI PpuMI PspOMI
PsrI PvuII SacI SapI SexAI SfcI SmlI SphI
SspI StuI StyI TatI TstI Tth111I XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BglI BglII BmgBI
BmtI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII
XbaI XcmI XmaI XmnI ZraI