Restriction map for
pF1KE2590
(Linear) (Six-base) MAPSORT of: KIEE2590.seq Check: 7567 from: 1 to: 2385
KIEE2590.orf
With 182 enzymes: SgfI *
September 30, 2015 09:49 ..
Acc65I G'GTAC_C
Cuts at: 0 1643 2327 2385
Size: 1643 684 58
Fragments arranged by size:
1643 684 58
AclI AA'CG_TT
Cuts at: 0 814 2385
Size: 814 1571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 849 1648 1795 1843 2004 2006 2385
Size: 499 350 799 147 48 161 2 379
Fragments arranged by size:
799 499 379 350 161 147 48 2
AflIII A'CryG_T
Cuts at: 0 746 2385
Size: 746 1639
AhdI GACnn_n'nnGTC
Cuts at: 0 2229 2385
Size: 2229 156
AlwNI CAG_nnn'CTG
Cuts at: 0 1605 2173 2385
Size: 1605 568 212
Fragments arranged by size:
1605 568 212
ApoI r'AATT_y
Cuts at: 0 1197 1278 2385
Size: 1197 81 1107
Fragments arranged by size:
1197 1107 81
AvaI C'yCGr_G
Cuts at: 0 496 1658 2212 2385
Size: 496 1162 554 173
Fragments arranged by size:
1162 554 496 173
BamHI G'GATC_C
Cuts at: 0 405 531 2385
Size: 405 126 1854
Fragments arranged by size:
1854 405 126
BanI G'GyrC_C
Cuts at: 0 174 1635 1643 2327 2385
Size: 174 1461 8 684 58
Fragments arranged by size:
1461 684 174 58 8
BanII G_rGCy'C
Cuts at: 0 680 760 2385
Size: 680 80 1625
Fragments arranged by size:
1625 680 80
BbeI G_GCGC'C
Cuts at: 0 1639 2385
Size: 1639 746
BbsI GAAGACnn'nnnn_
Cuts at: 0 960 1019 1118 1956 2385
Size: 960 59 99 838 429
Fragments arranged by size:
960 838 429 99 59
BbvCI CC'TCA_GC
Cuts at: 0 1811 2248 2385
Size: 1811 437 137
Fragments arranged by size:
1811 437 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1621 1655 1917 1951 2385
Size: 1621 34 262 34 434
Fragments arranged by size:
1621 434 262 34 34
BclI T'GATC_A
Cuts at: 0 23 2385
Size: 23 2362
BglI GCCn_nnn'nGGC
Cuts at: 0 55 2385
Size: 55 2330
Bme1580I G_kGCm'C
Cuts at: 0 179 2385
Size: 179 2206
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1346 1506 1830 2385
Size: 531 815 160 324 555
Fragments arranged by size:
815 555 531 324 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1545 2385
Size: 1545 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1934 1982 2311 2385
Size: 1934 48 329 74
Fragments arranged by size:
1934 329 74 48
Bpu10I CC'TnA_GC
Cuts at: 0 382 686 1811 2248 2323 2385
Size: 382 304 1125 437 75 62
Fragments arranged by size:
1125 437 382 304 75 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 2385
Size: 760 1625
BsaI GGTCTCn'nnnn_
Cuts at: 0 1400 2385
Size: 1400 985
BsaAI yAC'GTr
Cuts at: 0 1746 2220 2385
Size: 1746 474 165
Fragments arranged by size:
1746 474 165
BsaHI Gr'CG_yC
Cuts at: 0 1636 2385
Size: 1636 749
BsaWI w'CCGG_w
Cuts at: 0 731 1478 2385
Size: 731 747 907
Fragments arranged by size:
907 747 731
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 237 267 2385
Size: 237 30 2118
Fragments arranged by size:
2118 237 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 258 261 881 915 1111 1302 1591 2232
Size: 258 3 620 34 196 191 289 641
Cuts at: 2232 2310 2385
Size: 78 75
Fragments arranged by size:
641 620 289 258 196 191 78 75
34 3
BseYI C'CCAG_C
Cuts at: 0 36 58 190 413 1071 1372 2121 2385
Size: 36 22 132 223 658 301 749 264
Fragments arranged by size:
749 658 301 264 223 132 36 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1004 2385
Size: 170 834 1381
Fragments arranged by size:
1381 834 170
BsiHKAI G_wGCw'C
Cuts at: 0 316 2219 2385
Size: 316 1903 166
Fragments arranged by size:
1903 316 166
Bsp1286I G_dGCh'C
Cuts at: 0 179 316 680 760 2219 2385
Size: 179 137 364 80 1459 166
Fragments arranged by size:
1459 364 179 166 137 80
BspEI T'CCGG_A
Cuts at: 0 1478 2385
Size: 1478 907
BspHI T'CATG_A
Cuts at: 0 1799 2093 2385
Size: 1799 294 292
Fragments arranged by size:
1799 294 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 1269 2385
Size: 474 795 1116
Fragments arranged by size:
1116 795 474
BsrDI GCAATG_nn'
Cuts at: 0 2309 2385
Size: 2309 76
BstYI r'GATC_y
Cuts at: 0 405 531 1122 2036 2385
Size: 405 126 591 914 349
Fragments arranged by size:
914 591 405 349 126
BtsI GCAGTG_nn'
Cuts at: 0 347 564 2385
Size: 347 217 1821
Fragments arranged by size:
1821 347 217
EarI CTCTTCn'nnn_
Cuts at: 0 1224 1423 1495 1696 2242 2385
Size: 1224 199 72 201 546 143
Fragments arranged by size:
1224 546 201 199 143 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1115 2245 2385
Size: 1115 1130 140
Fragments arranged by size:
1130 1115 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 849 1648 1795 1843 1934 1982 2004
Size: 499 350 799 147 48 91 48 22
Cuts at: 2004 2006 2311 2385
Size: 2 305 74
Fragments arranged by size:
799 499 350 305 147 91 74 48
48 22 2
EcoO109I rG'GnC_Cy
Cuts at: 0 360 722 1340 2385
Size: 360 362 618 1045
Fragments arranged by size:
1045 618 362 360
EcoRV GAT'ATC
Cuts at: 0 149 2385
Size: 149 2236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1209 2385
Size: 468 741 1176
Fragments arranged by size:
1176 741 468
HaeII r_GCGC'y
Cuts at: 0 1605 1639 1980 2385
Size: 1605 34 341 405
Fragments arranged by size:
1605 405 341 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 454 486 577 609 2051 2083
Size: 133 32 289 32 91 32 1442 32
Cuts at: 2083 2215 2247 2356 2385
Size: 132 32 109 29
Fragments arranged by size:
1442 289 133 132 109 91 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 0 1611 2385
Size: 1611 774
KasI G'GCGC_C
Cuts at: 0 1635 2385
Size: 1635 750
KpnI G_GTAC'C
Cuts at: 0 1647 2331 2385
Size: 1647 684 54
Fragments arranged by size:
1647 684 54
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1296 1760 2385
Size: 1296 464 625
Fragments arranged by size:
1296 625 464
MspA1I CmG'CkG
Cuts at: 0 40 2199 2385
Size: 40 2159 186
Fragments arranged by size:
2159 186 40
NarI GG'CG_CC
Cuts at: 0 1636 2385
Size: 1636 749
NspI r_CATG'y
Cuts at: 0 826 2385
Size: 826 1559
PasI CC'CwG_GG
Cuts at: 0 725 2127 2385
Size: 725 1402 258
Fragments arranged by size:
1402 725 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 439 1518 1605 1862 2385
Size: 439 1079 87 257 523
Fragments arranged by size:
1079 523 439 257 87
PmlI CAC'GTG
Cuts at: 0 2220 2385
Size: 2220 165
PpuMI rG'GwC_Cy
Cuts at: 0 360 1340 2385
Size: 360 980 1045
Fragments arranged by size:
1045 980 360
PspXI vC'TCGA_Gb
Cuts at: 0 496 2212 2385
Size: 496 1716 173
Fragments arranged by size:
1716 496 173
PstI C_TGCA'G
Cuts at: 0 45 1025 2385
Size: 45 980 1360
Fragments arranged by size:
1360 980 45
PvuII CAG'CTG
Cuts at: 0 40 2385
Size: 40 2345
SanDI GG'GwC_CC
Cuts at: 0 1340 2385
Size: 1340 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 1696 2385
Size: 1696 689
ScaI AGT'ACT
Cuts at: 0 1699 2385
Size: 1699 686
SexAI A'CCwGG_T
Cuts at: 0 2261 2385
Size: 2261 124
SfcI C'TryA_G
Cuts at: 0 41 1021 2385
Size: 41 980 1364
Fragments arranged by size:
1364 980 41
SfoI GGC'GCC
Cuts at: 0 1637 2385
Size: 1637 748
SmaI CCC'GGG
Cuts at: 0 1660 2385
Size: 1660 725
SmlI C'TyrA_G
Cuts at: 0 496 739 2212 2385
Size: 496 243 1473 173
Fragments arranged by size:
1473 496 243 173
StyI C'CwwG_G
Cuts at: 0 1401 1856 2335 2385
Size: 1401 455 479 50
Fragments arranged by size:
1401 479 455 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 204 1774 2385
Size: 204 1570 611
Fragments arranged by size:
1570 611 204
TatI w'GTAC_w
Cuts at: 0 1697 2385
Size: 1697 688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 237 269 982 1014 2162 2194 2385
Size: 237 32 713 32 1148 32 191
Fragments arranged by size:
1148 713 237 191 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1119 2385
Size: 1119 1266
XhoI C'TCGA_G
Cuts at: 0 496 2212 2385
Size: 496 1716 173
Fragments arranged by size:
1716 496 173
XmaI C'CCGG_G
Cuts at: 0 1658 2385
Size: 1658 727
XmnI GAAnn'nnTTC
Cuts at: 0 1115 2385
Size: 1115 1270
Enzymes that do cut:
Acc65I AclI AcuI AflIII AhdI AlwNI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrDI BstYI BtsI EarI
EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII
KasI KpnI MmeI MspA1I NarI NspI PasI PflMI
PmlI PpuMI PspXI PstI PvuII SanDI SapI ScaI
SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI
TstI XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BciVI BfrBI BglII BlpI BmgBI BmtI BsaBI BsiEI
BsiWI BsmI BsmBI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoRI FseI FspI FspAI HindIII HpaI MfeI
MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XcmI ZraI