Restriction map for
pF1KE2585
(Linear) (Six-base) MAPSORT of: KIEE2585.seq Check: 3523 from: 1 to: 2514
KIEE2585.orf
With 182 enzymes: SgfI *
September 4, 2015 15:51 ..
AccI GT'mk_AC
Cuts at: 0 1201 2514
Size: 1201 1313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 373 1511 2514
Size: 343 30 1138 1003
Fragments arranged by size:
1138 1003 343 30
AflIII A'CryG_T
Cuts at: 0 1862 2514
Size: 1862 652
AgeI A'CCGG_T
Cuts at: 0 244 2455 2514
Size: 244 2211 59
Fragments arranged by size:
2211 244 59
AleI CACnn'nnGTG
Cuts at: 0 1953 2514
Size: 1953 561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 893 2514
Size: 893 1621
AlwNI CAG_nnn'CTG
Cuts at: 0 61 1888 2514
Size: 61 1827 626
Fragments arranged by size:
1827 626 61
ApaI G_GGCC'C
Cuts at: 0 2232 2514
Size: 2232 282
ApaLI G'TGCA_C
Cuts at: 0 664 2514
Size: 664 1850
ApoI r'AATT_y
Cuts at: 0 148 385 1922 2440 2514
Size: 148 237 1537 518 74
Fragments arranged by size:
1537 518 237 148 74
AvaI C'yCGr_G
Cuts at: 0 272 2514
Size: 272 2242
BamHI G'GATC_C
Cuts at: 0 450 642 1307 2514
Size: 450 192 665 1207
Fragments arranged by size:
1207 665 450 192
BanI G'GyrC_C
Cuts at: 0 992 2514
Size: 992 1522
BanII G_rGCy'C
Cuts at: 0 2232 2514
Size: 2232 282
BbvCI CC'TCA_GC
Cuts at: 0 2233 2514
Size: 2233 281
BclI T'GATC_A
Cuts at: 0 2375 2514
Size: 2375 139
Bme1580I G_kGCm'C
Cuts at: 0 668 997 1042 2232 2514
Size: 668 329 45 1190 282
Fragments arranged by size:
1190 668 329 282 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 2430 2514
Size: 2430 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 766 2406 2514
Size: 766 1640 108
Fragments arranged by size:
1640 766 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 818 1476 1562 2514
Size: 658 160 658 86 952
Fragments arranged by size:
952 658 658 160 86
Bpu10I CC'TnA_GC
Cuts at: 0 2233 2293 2514
Size: 2233 60 221
Fragments arranged by size:
2233 221 60
BsaAI yAC'GTr
Cuts at: 0 663 2514
Size: 663 1851
BsaBI GATnn'nnATC
Cuts at: 0 2284 2514
Size: 2284 230
BsaWI w'CCGG_w
Cuts at: 0 14 244 2455 2514
Size: 14 230 2211 59
Fragments arranged by size:
2211 230 59 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 997 1027 2089 2119 2194 2224 2306 2336
Size: 997 30 1062 30 75 30 82 30
Cuts at: 2336 2514
Size: 178
Fragments arranged by size:
1062 997 178 82 75 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2025 2086 2514
Size: 2025 61 428
Fragments arranged by size:
2025 428 61
BseYI C'CCAG_C
Cuts at: 0 38 1530 2514
Size: 38 1492 984
Fragments arranged by size:
1492 984 38
BsiEI CG_ry'CG
Cuts at: 0 1468 2514
Size: 1468 1046
BsiHKAI G_wGCw'C
Cuts at: 0 668 2514
Size: 668 1846
BsmI GAATG_Cn'
Cuts at: 0 1836 2514
Size: 1836 678
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1489 2514
Size: 1489 1025
Bsp1286I G_dGCh'C
Cuts at: 0 668 997 1042 2232 2514
Size: 668 329 45 1190 282
Fragments arranged by size:
1190 668 329 282 45
BspEI T'CCGG_A
Cuts at: 0 14 2514
Size: 14 2500
BspHI T'CATG_A
Cuts at: 0 1754 2514
Size: 1754 760
BsrDI GCAATG_nn'
Cuts at: 0 1589 2514
Size: 1589 925
BsrFI r'CCGG_y
Cuts at: 0 244 2455 2514
Size: 244 2211 59
Fragments arranged by size:
2211 244 59
BsrGI T'GTAC_A
Cuts at: 0 1394 2514
Size: 1394 1120
BstYI r'GATC_y
Cuts at: 0 450 642 1307 2514
Size: 450 192 665 1207
Fragments arranged by size:
1207 665 450 192
BstZ17I GTA'TAC
Cuts at: 0 1202 2514
Size: 1202 1312
BtgI C'CryG_G
Cuts at: 0 2150 2273 2360 2514
Size: 2150 123 87 154
Fragments arranged by size:
2150 154 123 87
BtsI GCAGTG_nn'
Cuts at: 0 2204 2339 2407 2514
Size: 2204 135 68 107
Fragments arranged by size:
2204 135 107 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2022 2057 2514
Size: 2022 35 457
Fragments arranged by size:
2022 457 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2152 2514
Size: 2152 362
EaeI y'GGCC_r
Cuts at: 0 1465 2363 2514
Size: 1465 898 151
Fragments arranged by size:
1465 898 151
EagI C'GGCC_G
Cuts at: 0 1465 2514
Size: 1465 1049
EarI CTCTTCn'nnn_
Cuts at: 0 349 461 498 1487 2296 2504 2514
Size: 349 112 37 989 809 208 10
Fragments arranged by size:
989 809 349 208 112 37 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 2514
Size: 463 2051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 373 658 818 1476 1511 1562 2514
Size: 343 30 285 160 658 35 51 952
Fragments arranged by size:
952 658 343 285 160 51 35 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 2514
Size: 613 1901
EcoO109I rG'GnC_Cy
Cuts at: 0 2229 2385 2514
Size: 2229 156 129
Fragments arranged by size:
2229 156 129
EcoRI G'AATT_C
Cuts at: 0 1922 2514
Size: 1922 592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 1516 2514
Size: 378 1138 998
Fragments arranged by size:
1138 998 378
FspI TGC'GCA
Cuts at: 0 2249 2514
Size: 2249 265
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 460 467 492 804 836 997 1029
Size: 435 25 7 25 312 32 161 32
Cuts at: 1029 2089 2121 2514
Size: 1060 32 393
Fragments arranged by size:
1060 435 393 312 161 32 32 32
25 25 7
HindIII A'AGCT_T
Cuts at: 0 190 2514
Size: 190 2324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 443 2225 2514
Size: 165 278 1782 289
Fragments arranged by size:
1782 289 278 165
MscI TGG'CCA
Cuts at: 0 2365 2514
Size: 2365 149
MslI CAynn'nnrTG
Cuts at: 0 907 958 1264 1660 1861 1953 2514
Size: 907 51 306 396 201 92 561
Fragments arranged by size:
907 561 396 306 201 92 51
MspA1I CmG'CkG
Cuts at: 0 705 877 1524 2253 2450 2514
Size: 705 172 647 729 197 64
Fragments arranged by size:
729 705 647 197 172 64
NcoI C'CATG_G
Cuts at: 0 2150 2273 2514
Size: 2150 123 241
Fragments arranged by size:
2150 241 123
NdeI CA'TA_TG
Cuts at: 0 1661 2514
Size: 1661 853
NspI r_CATG'y
Cuts at: 0 579 1866 2514
Size: 579 1287 648
Fragments arranged by size:
1287 648 579
PciI A'CATG_T
Cuts at: 0 1862 2514
Size: 1862 652
PflMI CCAn_nnn'nTGG
Cuts at: 0 1888 2514
Size: 1888 626
PfoI T'CCnGG_A
Cuts at: 0 365 1453 2514
Size: 365 1088 1061
Fragments arranged by size:
1088 1061 365
PshAI GACnn'nnGTC
Cuts at: 0 1250 2514
Size: 1250 1264
PsiI TTA'TAA
Cuts at: 0 1481 2514
Size: 1481 1033
PspOMI G'GGCC_C
Cuts at: 0 2228 2514
Size: 2228 286
PstI C_TGCA'G
Cuts at: 0 872 2514
Size: 872 1642
PvuII CAG'CTG
Cuts at: 0 705 877 1524 2253 2514
Size: 705 172 647 729 261
Fragments arranged by size:
729 705 647 261 172
RsrII CG'GwC_CG
Cuts at: 0 2062 2514
Size: 2062 452
SapI GCTCTTCn'nnn_
Cuts at: 0 2296 2514
Size: 2296 218
ScaI AGT'ACT
Cuts at: 0 2182 2514
Size: 2182 332
SfcI C'TryA_G
Cuts at: 0 49 868 1519 2445 2514
Size: 49 819 651 926 69
Fragments arranged by size:
926 819 651 69 49
SgrAI Cr'CCGG_yG
Cuts at: 0 244 2514
Size: 244 2270
SmaI CCC'GGG
Cuts at: 0 274 2514
Size: 274 2240
SphI G_CATG'C
Cuts at: 0 579 2514
Size: 579 1935
StyI C'CwwG_G
Cuts at: 0 693 1647 1963 2150 2273 2514
Size: 693 954 316 187 123 241
Fragments arranged by size:
954 693 316 241 187 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 1240 1369 2079 2125 2514
Size: 391 849 129 710 46 389
Fragments arranged by size:
849 710 391 389 129 46
TatI w'GTAC_w
Cuts at: 0 1394 2180 2514
Size: 1394 786 334
Fragments arranged by size:
1394 786 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 2306 2338 2514
Size: 1039 32 1235 32 176
Fragments arranged by size:
1235 1039 176 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1666 2358 2514
Size: 1666 692 156
Fragments arranged by size:
1666 692 156
XmaI C'CCGG_G
Cuts at: 0 272 2514
Size: 272 2242
XmnI GAAnn'nnTTC
Cuts at: 0 136 2440 2514
Size: 136 2304 74
Fragments arranged by size:
2304 136 74
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbvCI BclI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BsrDI BsrFI BsrGI BstYI BstZ17I BtgI
BtsI CspCI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NdeI NspI PciI PflMI
PfoI PshAI PsiI PspOMI PstI PvuII RsrII SapI
ScaI SfcI SgrAI SmaI SphI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AloI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI
BsaI BsaHI BsgI BsiWI BspMI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI DraI
DraIII EcoICRI EcoRV FseI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PpuMI PspXI PsrI PvuI SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SmlI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI