Restriction map for
pF1KE2580
(Linear) (Six-base) MAPSORT of: KIEE2580.seq Check: 381 from: 1 to: 3582
KIEE2580.orf
With 182 enzymes: SgfI *
July 27, 2015 09:05 ..
AccI GT'mk_AC
Cuts at: 0 716 2917 3582
Size: 716 2201 665
Fragments arranged by size:
2201 716 665
Acc65I G'GTAC_C
Cuts at: 0 2015 3582
Size: 2015 1567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1421 3582
Size: 390 1031 2161
Fragments arranged by size:
2161 1031 390
AflIII A'CryG_T
Cuts at: 0 1772 3582
Size: 1772 1810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 2121 3241 3582
Size: 1357 764 1120 341
Fragments arranged by size:
1357 1120 764 341
AlwNI CAG_nnn'CTG
Cuts at: 0 3012 3192 3582
Size: 3012 180 390
Fragments arranged by size:
3012 390 180
ApoI r'AATT_y
Cuts at: 0 16 488 770 1537 1543 1828 1990 2209
Size: 16 472 282 767 6 285 162 219
Cuts at: 2209 2518 2817 3002 3433 3582
Size: 309 299 185 431 149
Fragments arranged by size:
767 472 431 309 299 285 282 219
185 162 149 16 6
BamHI G'GATC_C
Cuts at: 0 225 2354 3582
Size: 225 2129 1228
Fragments arranged by size:
2129 1228 225
BanI G'GyrC_C
Cuts at: 0 2015 2155 3582
Size: 2015 140 1427
Fragments arranged by size:
2015 1427 140
BanII G_rGCy'C
Cuts at: 0 602 2678 3582
Size: 602 2076 904
Fragments arranged by size:
2076 904 602
BbsI GAAGACnn'nnnn_
Cuts at: 0 562 903 1013 1106 1769 1819 2765 3582
Size: 562 341 110 93 663 50 946 817
Fragments arranged by size:
946 817 663 562 341 110 93 50
BbvCI CC'TCA_GC
Cuts at: 0 3098 3127 3582
Size: 3098 29 455
Fragments arranged by size:
3098 455 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 990 1024 1840 1874 3487 3521 3582
Size: 990 34 816 34 1613 34 61
Fragments arranged by size:
1613 990 816 61 34 34 34
BclI T'GATC_A
Cuts at: 0 1703 3080 3582
Size: 1703 1377 502
Fragments arranged by size:
1703 1377 502
BglI GCCn_nnn'nGGC
Cuts at: 0 799 3582
Size: 799 2783
BglII A'GATC_T
Cuts at: 0 312 669 1275 3582
Size: 312 357 606 2307
Fragments arranged by size:
2307 606 357 312
Bme1580I G_kGCm'C
Cuts at: 0 2597 3582
Size: 2597 985
BmgBI CAC'GTC
Cuts at: 0 2592 3582
Size: 2592 990
BmrI ACTGGGnnnn_n'
Cuts at: 0 569 901 2591 3582
Size: 569 332 1690 991
Fragments arranged by size:
1690 991 569 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1469 3582
Size: 1469 2113
Bpu10I CC'TnA_GC
Cuts at: 0 3098 3127 3582
Size: 3098 29 455
Fragments arranged by size:
3098 455 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 541 2328 2729 3582
Size: 424 117 1787 401 853
Fragments arranged by size:
1787 853 424 401 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 3110 3582
Size: 3110 472
BsaAI yAC'GTr
Cuts at: 0 2163 2203 3582
Size: 2163 40 1379
Fragments arranged by size:
2163 1379 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1031 1061 1868 1898 2477 2507 3582
Size: 1031 30 807 30 579 30 1075
Fragments arranged by size:
1075 1031 807 579 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1510 1534 3115 3582
Size: 94 1416 24 1581 467
Fragments arranged by size:
1581 1416 467 94 24
BseYI C'CCAG_C
Cuts at: 0 1082 1464 1715 3185 3582
Size: 1082 382 251 1470 397
Fragments arranged by size:
1470 1082 397 382 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2134 2856 3106 3582
Size: 2134 722 250 476
Fragments arranged by size:
2134 722 476 250
BsiEI CG_ry'CG
Cuts at: 0 208 3582
Size: 208 3374
BsiHKAI G_wGCw'C
Cuts at: 0 2678 3234 3582
Size: 2678 556 348
Fragments arranged by size:
2678 556 348
BsmI GAATG_Cn'
Cuts at: 0 187 3582
Size: 187 3395
Bsp1286I G_dGCh'C
Cuts at: 0 602 2597 2678 3234 3582
Size: 602 1995 81 556 348
Fragments arranged by size:
1995 602 556 348 81
BspHI T'CATG_A
Cuts at: 0 2036 2922 3582
Size: 2036 886 660
Fragments arranged by size:
2036 886 660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2106 3128 3582
Size: 2106 1022 454
Fragments arranged by size:
2106 1022 454
BsrDI GCAATG_nn'
Cuts at: 0 376 809 1139 2951 3582
Size: 376 433 330 1812 631
Fragments arranged by size:
1812 631 433 376 330
BsrGI T'GTAC_A
Cuts at: 0 1034 1138 3582
Size: 1034 104 2444
Fragments arranged by size:
2444 1034 104
BssSI C'ACGA_G
Cuts at: 0 3172 3582
Size: 3172 410
BstBI TT'CG_AA
Cuts at: 0 2403 3582
Size: 2403 1179
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 3396 3582
Size: 237 3159 186
Fragments arranged by size:
3159 237 186
BstYI r'GATC_y
Cuts at: 0 225 312 669 753 1275 1287 2354 2508
Size: 225 87 357 84 522 12 1067 154
Cuts at: 2508 3582
Size: 1074
Fragments arranged by size:
1074 1067 522 357 225 154 87 84
12
BtgI C'CryG_G
Cuts at: 0 2690 3582
Size: 2690 892
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1984 3582
Size: 1984 1598
BtsI GCAGTG_nn'
Cuts at: 0 309 1230 2868 3142 3485 3582
Size: 309 921 1638 274 343 97
Fragments arranged by size:
1638 921 343 309 274 97
DraI TTT'AAA
Cuts at: 0 2795 2825 3582
Size: 2795 30 757
Fragments arranged by size:
2795 757 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 2302 3582
Size: 2302 1280
EaeI y'GGCC_r
Cuts at: 0 2 2346 3582
Size: 2 2344 1236
Fragments arranged by size:
2344 1236 2
EarI CTCTTCn'nnn_
Cuts at: 0 118 1487 2255 3582
Size: 118 1369 768 1327
Fragments arranged by size:
1369 1327 768 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 300 3582
Size: 300 3282
EcoICRI GAG'CTC
Cuts at: 0 2676 3582
Size: 2676 906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1421 1469 3582
Size: 390 1031 48 2113
Fragments arranged by size:
2113 1031 390 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1153 2938 3582
Size: 1153 1785 644
Fragments arranged by size:
1785 1153 644
EcoO109I rG'GnC_Cy
Cuts at: 0 1611 3582
Size: 1611 1971
EcoRI G'AATT_C
Cuts at: 0 1537 2209 3433 3582
Size: 1537 672 1224 149
Fragments arranged by size:
1537 1224 672 149
EcoRV GAT'ATC
Cuts at: 0 883 1146 2386 2490 3582
Size: 883 263 1240 104 1092
Fragments arranged by size:
1240 1092 883 263 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 2799 3436 3582
Size: 561 2238 637 146
Fragments arranged by size:
2238 637 561 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 636 668 1031 1063 1868 1900
Size: 70 32 534 32 363 32 805 32
Cuts at: 1900 2053 2085 2488 2520 3006 3013 3038 3045
Size: 153 32 403 32 486 7 25 7
Cuts at: 3045 3582
Size: 537
Fragments arranged by size:
805 537 534 486 403 363 153 70
32 32 32 32 32 32 25 7
7
HincII GTy'rAC
Cuts at: 0 2883 2918 3582
Size: 2883 35 664
Fragments arranged by size:
2883 664 35
HindIII A'AGCT_T
Cuts at: 0 1424 3582
Size: 1424 2158
KpnI G_GTAC'C
Cuts at: 0 2019 3582
Size: 2019 1563
MfeI C'AATT_G
Cuts at: 0 1823 1975 3582
Size: 1823 152 1607
Fragments arranged by size:
1823 1607 152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 3582
Size: 237 3345
MscI TGG'CCA
Cuts at: 0 4 2348 3582
Size: 4 2344 1234
Fragments arranged by size:
2344 1234 4
MslI CAynn'nnrTG
Cuts at: 0 817 2227 3582
Size: 817 1410 1355
Fragments arranged by size:
1410 1355 817
MspA1I CmG'CkG
Cuts at: 0 1419 3192 3582
Size: 1419 1773 390
Fragments arranged by size:
1773 1419 390
NdeI CA'TA_TG
Cuts at: 0 2466 3582
Size: 2466 1116
NspI r_CATG'y
Cuts at: 0 437 1776 1822 1905 2839 3582
Size: 437 1339 46 83 934 743
Fragments arranged by size:
1339 934 743 437 83 46
PciI A'CATG_T
Cuts at: 0 1772 3582
Size: 1772 1810
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 954 3582
Size: 942 12 2628
Fragments arranged by size:
2628 942 12
PshAI GACnn'nnGTC
Cuts at: 0 1574 3582
Size: 1574 2008
PsiI TTA'TAA
Cuts at: 0 1655 1769 1841 3582
Size: 1655 114 72 1741
Fragments arranged by size:
1741 1655 114 72
PstI C_TGCA'G
Cuts at: 0 304 1237 3582
Size: 304 933 2345
Fragments arranged by size:
2345 933 304
PvuII CAG'CTG
Cuts at: 0 1419 3192 3582
Size: 1419 1773 390
Fragments arranged by size:
1773 1419 390
SacI G_AGCT'C
Cuts at: 0 2678 3582
Size: 2678 904
SalI G'TCGA_C
Cuts at: 0 2916 3582
Size: 2916 666
SfcI C'TryA_G
Cuts at: 0 300 1233 1622 3582
Size: 300 933 389 1960
Fragments arranged by size:
1960 933 389 300
SmlI C'TyrA_G
Cuts at: 0 439 556 2307 2744 3582
Size: 439 117 1751 437 838
Fragments arranged by size:
1751 838 439 437 117
SnaBI TAC'GTA
Cuts at: 0 2163 2203 3582
Size: 2163 40 1379
Fragments arranged by size:
2163 1379 40
SpeI A'CTAG_T
Cuts at: 0 2575 3582
Size: 2575 1007
SspI AAT'ATT
Cuts at: 0 1267 1761 2451 3582
Size: 1267 494 690 1131
Fragments arranged by size:
1267 1131 690 494
StuI AGG'CCT
Cuts at: 0 383 854 3582
Size: 383 471 2728
Fragments arranged by size:
2728 471 383
StyI C'CwwG_G
Cuts at: 0 1871 3582
Size: 1871 1711
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 194 1786 3582
Size: 194 1592 1796
Fragments arranged by size:
1796 1592 194
TatI w'GTAC_w
Cuts at: 0 1034 1138 1250 3582
Size: 1034 104 112 2332
Fragments arranged by size:
2332 1034 112 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 279 311 2477 2509 3582
Size: 279 32 2166 32 1073
Fragments arranged by size:
2166 1073 279 32 32
XbaI T'CTAG_A
Cuts at: 0 3429 3582
Size: 3429 153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2697 3582
Size: 2697 885
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApoI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NdeI NspI PciI PflMI PshAI PsiI PstI PvuII
SacI SalI SfcI SmlI SnaBI SpeI SspI StuI
StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BciVI BfrBI BlpI BmtI BplI BsaBI
BsaHI BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EagI
FseI FspI FspAI HaeII HpaI KasI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI Tth111I XhoI XmaI XmnI ZraI