Restriction map for
pF1KE2577
(Linear) (Six-base) MAPSORT of: KIEE2577.seq Check: 585 from: 1 to: 969
KIEE2577.orf
With 182 enzymes: SgfI *
June 12, 2015 15:10 ..
AccI GT'mk_AC
Cuts at: 0 110 969
Size: 110 859
Acc65I G'GTAC_C
Cuts at: 0 674 969
Size: 674 295
AvaI C'yCGr_G
Cuts at: 0 758 793 949 969
Size: 758 35 156 20
Fragments arranged by size:
758 156 35 20
BanI G'GyrC_C
Cuts at: 0 251 674 969
Size: 251 423 295
Fragments arranged by size:
423 295 251
BanII G_rGCy'C
Cuts at: 0 793 969
Size: 793 176
BclI T'GATC_A
Cuts at: 0 842 926 969
Size: 842 84 43
Fragments arranged by size:
842 84 43
BglI GCCn_nnn'nGGC
Cuts at: 0 210 730 969
Size: 210 520 239
Fragments arranged by size:
520 239 210
BlpI GC'TnA_GC
Cuts at: 0 824 969
Size: 824 145
Bme1580I G_kGCm'C
Cuts at: 0 254 969
Size: 254 715
BmrI ACTGGGnnnn_n'
Cuts at: 0 848 969
Size: 848 121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 452 969
Size: 99 353 517
Fragments arranged by size:
517 353 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 838 969
Size: 94 744 131
Fragments arranged by size:
744 131 94
Bpu10I CC'TnA_GC
Cuts at: 0 787 969
Size: 787 182
BsaHI Gr'CG_yC
Cuts at: 0 782 969
Size: 782 187
BseYI C'CCAG_C
Cuts at: 0 175 932 969
Size: 175 757 37
Fragments arranged by size:
757 175 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 969
Size: 700 269
BsiHKAI G_wGCw'C
Cuts at: 0 80 809 969
Size: 80 729 160
Fragments arranged by size:
729 160 80
BsmI GAATG_Cn'
Cuts at: 0 170 527 969
Size: 170 357 442
Fragments arranged by size:
442 357 170
Bsp1286I G_dGCh'C
Cuts at: 0 80 254 793 809 969
Size: 80 174 539 16 160
Fragments arranged by size:
539 174 160 80 16
BsrDI GCAATG_nn'
Cuts at: 0 186 969
Size: 186 783
BsrFI r'CCGG_y
Cuts at: 0 587 724 969
Size: 587 137 245
Fragments arranged by size:
587 245 137
BsrGI T'GTAC_A
Cuts at: 0 571 969
Size: 571 398
BstAPI GCAn_nnn'nTGC
Cuts at: 0 599 804 969
Size: 599 205 165
Fragments arranged by size:
599 205 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 259 969
Size: 259 710
ClaI AT'CG_AT
Cuts at: 0 578 969
Size: 578 391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 723 758 969
Size: 723 35 211
Fragments arranged by size:
723 211 35
DraI TTT'AAA
Cuts at: 0 519 969
Size: 519 450
EaeI y'GGCC_r
Cuts at: 0 238 747 902 969
Size: 238 509 155 67
Fragments arranged by size:
509 238 155 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 838 969
Size: 94 744 131
Fragments arranged by size:
744 131 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 817 969
Size: 817 152
EcoRV GAT'ATC
Cuts at: 0 475 969
Size: 475 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 969
Size: 451 32 486
Fragments arranged by size:
486 451 32
KpnI G_GTAC'C
Cuts at: 0 678 969
Size: 678 291
MscI TGG'CCA
Cuts at: 0 240 969
Size: 240 729
MslI CAynn'nnrTG
Cuts at: 0 293 883 969
Size: 293 590 86
Fragments arranged by size:
590 293 86
MspA1I CmG'CkG
Cuts at: 0 714 932 969
Size: 714 218 37
Fragments arranged by size:
714 218 37
NaeI GCC'GGC
Cuts at: 0 726 969
Size: 726 243
NgoMIV G'CCGG_C
Cuts at: 0 724 969
Size: 724 245
PflMI CCAn_nnn'nTGG
Cuts at: 0 247 969
Size: 247 722
PfoI T'CCnGG_A
Cuts at: 0 655 815 969
Size: 655 160 154
Fragments arranged by size:
655 160 154
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 332 364 969
Size: 332 32 605
Fragments arranged by size:
605 332 32
PstI C_TGCA'G
Cuts at: 0 55 457 969
Size: 55 402 512
Fragments arranged by size:
512 402 55
PvuII CAG'CTG
Cuts at: 0 714 932 969
Size: 714 218 37
Fragments arranged by size:
714 218 37
SfcI C'TryA_G
Cuts at: 0 51 144 453 969
Size: 51 93 309 516
Fragments arranged by size:
516 309 93 51
SmaI CCC'GGG
Cuts at: 0 951 969
Size: 951 18
StuI AGG'CCT
Cuts at: 0 511 607 874 969
Size: 511 96 267 95
Fragments arranged by size:
511 267 96 95
TatI w'GTAC_w
Cuts at: 0 571 969
Size: 571 398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 369 401 969
Size: 369 32 568
Fragments arranged by size:
568 369 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 233 969
Size: 69 164 736
Fragments arranged by size:
736 164 69
XmaI C'CCGG_G
Cuts at: 0 949 969
Size: 949 20
Enzymes that do cut:
AccI Acc65I AvaI BanI BanII BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaHI BseYI BsgI
BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI BstAPI BtgZI
ClaI CspCI DraI EaeI Eco57MI EcoNI EcoRV Hin4I
KpnI MscI MslI MspA1I NaeI NgoMIV PflMI PfoI
PsrI PstI PvuII SfcI SmaI StuI TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII
DrdI EagI EarI EciI EcoICRI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII Tth111I XbaI XhoI
XmnI ZraI