(Linear) (Six-base) MAPSORT of: KIEE2576.seq  Check: 6377  from: 1  to: 507



KIEE2576.orf

 With 182 enzymes: SgfI * 

                        May 26, 2015 10:14  ..

AccI GT'mk_AC

Cuts at:      0     452     507 
   Size:        452      55
 
AflIII A'CryG_T

Cuts at:      0     413     507 
   Size:        413      94
 
BanI G'GyrC_C

Cuts at:      0       3     277     337     507 
   Size:          3     274      60     170

  Fragments arranged by size: 

                 274     170      60       3
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      45     237     507 
   Size:         45     192     270

  Fragments arranged by size: 

                 270     192      45
 
BclI T'GATC_A

Cuts at:      0     296     507 
   Size:        296     211
 
Bme1580I G_kGCm'C

Cuts at:      0     340     507 
   Size:        340     167
 
BmgBI CAC'GTC

Cuts at:      0     438     507 
   Size:        438      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     379     507 
   Size:         30     349     128

  Fragments arranged by size: 

                 349     128      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     507 
   Size:        256     251
 
BsaHI Gr'CG_yC

Cuts at:      0     491     507 
   Size:        491      16
 
BsaWI w'CCGG_w

Cuts at:      0     301     507 
   Size:        301     206
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     376     406     507 
   Size:        376      30     101

  Fragments arranged by size: 

                 376     101      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     376     415     507 
   Size:        208     168      39      92

  Fragments arranged by size: 

                 208     168      92      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     340     507 
   Size:        340     167
 
BstYI r'GATC_y

Cuts at:      0      77     507 
   Size:         77     430
 
Bsu36I CC'TnA_GG

Cuts at:      0     287     326     507 
   Size:        287      39     181

  Fragments arranged by size: 

                 287     181      39
 
BtgI C'CryG_G

Cuts at:      0     167     507 
   Size:        167     340
 
BtsI GCAGTG_nn'

Cuts at:      0     385     507 
   Size:        385     122
 
DraIII CAC_nnn'GTG

Cuts at:      0     380     507 
   Size:        380     127
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      53     507 
   Size:         53     454
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     379     507 
   Size:         30     349     128

  Fragments arranged by size: 

                 349     128      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144     507 
   Size:        144     363
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     507 
   Size:        376      32      99

  Fragments arranged by size: 

                 376      99      32
 
HincII GTy'rAC

Cuts at:      0     453     507 
   Size:        453      54
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     507 
   Size:        179     328
 
MspA1I CmG'CkG

Cuts at:      0     224     507 
   Size:        224     283
 
NcoI C'CATG_G

Cuts at:      0     167     507 
   Size:        167     340
 
NspI r_CATG'y

Cuts at:      0     417     507 
   Size:        417      90
 
PciI A'CATG_T

Cuts at:      0     413     507 
   Size:        413      94
 
PfoI T'CCnGG_A

Cuts at:      0      80     356     507 
   Size:         80     276     151

  Fragments arranged by size: 

                 276     151      80
 
PpuMI rG'GwC_Cy

Cuts at:      0     144     507 
   Size:        144     363
 
SalI G'TCGA_C

Cuts at:      0     451     507 
   Size:        451      56
 
SmlI C'TyrA_G

Cuts at:      0     271     507 
   Size:        271     236
 
StuI AGG'CCT

Cuts at:      0      97     507 
   Size:         97     410
 
StyI C'CwwG_G

Cuts at:      0     167     507 
   Size:        167     340
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     507 
   Size:         21     486
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     121     507 
   Size:        121     386
 
XmnI GAAnn'nnTTC

Cuts at:      0     352     507 
   Size:        352     155
 

 Enzymes that do cut:

     AccI   AflIII     BanI     BbsI     BclI Bme1580I    BmgBI     BpmI
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI Bsp1286I    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EciI  Eco57MI EcoO109I    Hin4I   HincII
     MmeI   MspA1I     NcoI     NspI     PciI     PfoI    PpuMI     SalI
     SmlI     StuI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI