Restriction map for
pF1KE2570
(Linear) (Six-base) MAPSORT of: KIEE2570.seq Check: 6308 from: 1 to: 1578
KIEE2570.orf
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1260 1578
Size: 1207 53 318
Fragments arranged by size:
1207 318 53
AhdI GACnn_n'nnGTC
Cuts at: 0 743 1578
Size: 743 835
AleI CACnn'nnGTG
Cuts at: 0 316 1578
Size: 316 1262
ApoI r'AATT_y
Cuts at: 0 632 1578
Size: 632 946
AvaI C'yCGr_G
Cuts at: 0 609 684 1578
Size: 609 75 894
Fragments arranged by size:
894 609 75
BanII G_rGCy'C
Cuts at: 0 367 805 850 1578
Size: 367 438 45 728
Fragments arranged by size:
728 438 367 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1571 1578
Size: 1571 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 264 1578
Size: 264 1314
BlpI GC'TnA_GC
Cuts at: 0 287 1578
Size: 287 1291
BmrI ACTGGGnnnn_n'
Cuts at: 0 298 933 1578
Size: 298 635 645
Fragments arranged by size:
645 635 298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 726 834 1578
Size: 128 598 108 744
Fragments arranged by size:
744 598 128 108
Bpu10I CC'TnA_GC
Cuts at: 0 336 1578
Size: 336 1242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 1578
Size: 964 614
BsaI GGTCTCn'nnnn_
Cuts at: 0 832 1447 1578
Size: 832 615 131
Fragments arranged by size:
832 615 131
BsaWI w'CCGG_w
Cuts at: 0 1254 1578
Size: 1254 324
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 882 912 1578
Size: 882 30 666
Fragments arranged by size:
882 666 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 1273 1315 1411 1573 1578
Size: 341 932 42 96 162 5
Fragments arranged by size:
932 341 162 96 42 5
BseYI C'CCAG_C
Cuts at: 0 182 1503 1578
Size: 182 1321 75
Fragments arranged by size:
1321 182 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 811 1025 1578
Size: 404 407 214 553
Fragments arranged by size:
553 407 404 214
BsiHKAI G_wGCw'C
Cuts at: 0 193 376 850 1578
Size: 193 183 474 728
Fragments arranged by size:
728 474 193 183
BsmI GAATG_Cn'
Cuts at: 0 102 1578
Size: 102 1476
Bsp1286I G_dGCh'C
Cuts at: 0 193 367 376 805 850 1578
Size: 193 174 9 429 45 728
Fragments arranged by size:
728 429 193 174 45 9
BspHI T'CATG_A
Cuts at: 0 912 1578
Size: 912 666
BsrBI CCG'CTC
Cuts at: 0 1182 1578
Size: 1182 396
BssHII G'CGCG_C
Cuts at: 0 8 1578
Size: 8 1570
BssSI C'ACGA_G
Cuts at: 0 798 1327 1578
Size: 798 529 251
Fragments arranged by size:
798 529 251
BstEII G'GTnAC_C
Cuts at: 0 1420 1578
Size: 1420 158
BstYI r'GATC_y
Cuts at: 0 939 1578
Size: 939 639
BtgI C'CryG_G
Cuts at: 0 317 1124 1578
Size: 317 807 454
Fragments arranged by size:
807 454 317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 381 416 635 670 1578
Size: 381 35 219 35 908
Fragments arranged by size:
908 381 219 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 495 961 1578
Size: 495 466 617
Fragments arranged by size:
617 495 466
EaeI y'GGCC_r
Cuts at: 0 593 1578
Size: 593 985
EarI CTCTTCn'nnn_
Cuts at: 0 1464 1578
Size: 1464 114
EcoICRI GAG'CTC
Cuts at: 0 848 1578
Size: 848 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 726 834 1207 1260 1578
Size: 128 598 108 373 53 318
Fragments arranged by size:
598 373 318 128 108 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1540 1555 1578
Size: 1540 15 23
Fragments arranged by size:
1540 23 15
HaeII r_GCGC'y
Cuts at: 0 19 28 1016 1362 1578
Size: 19 9 988 346 216
Fragments arranged by size:
988 346 216 19 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 525 528 557 729 761 1509 1541
Size: 496 29 3 29 172 32 748 32
Cuts at: 1541 1578
Size: 37
Fragments arranged by size:
748 496 172 37 32 32 29 29
3
HincII GTy'rAC
Cuts at: 0 44 309 1578
Size: 44 265 1269
Fragments arranged by size:
1269 265 44
MscI TGG'CCA
Cuts at: 0 595 1578
Size: 595 983
MslI CAynn'nnrTG
Cuts at: 0 163 316 1041 1578
Size: 163 153 725 537
Fragments arranged by size:
725 537 163 153
MspA1I CmG'CkG
Cuts at: 0 54 1386 1514 1578
Size: 54 1332 128 64
Fragments arranged by size:
1332 128 64 54
NspI r_CATG'y
Cuts at: 0 1284 1578
Size: 1284 294
PfoI T'CCnGG_A
Cuts at: 0 1110 1578
Size: 1110 468
PshAI GACnn'nnGTC
Cuts at: 0 1126 1578
Size: 1126 452
PstI C_TGCA'G
Cuts at: 0 1406 1578
Size: 1406 172
PvuII CAG'CTG
Cuts at: 0 1514 1578
Size: 1514 64
SacI G_AGCT'C
Cuts at: 0 850 1578
Size: 850 728
SfcI C'TryA_G
Cuts at: 0 170 395 1402 1578
Size: 170 225 1007 176
Fragments arranged by size:
1007 225 176 170
SmaI CCC'GGG
Cuts at: 0 686 1578
Size: 686 892
SmlI C'TyrA_G
Cuts at: 0 943 1578
Size: 943 635
SphI G_CATG'C
Cuts at: 0 1284 1578
Size: 1284 294
StyI C'CwwG_G
Cuts at: 0 206 246 1166 1541 1578
Size: 206 40 920 375 37
Fragments arranged by size:
920 375 206 40 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1305 1548 1578
Size: 219 1086 243 30
Fragments arranged by size:
1086 243 219 30
XmaI C'CCGG_G
Cuts at: 0 684 1578
Size: 684 894
Enzymes that do cut:
AcuI AhdI AleI ApoI AvaI BanII BbsI BciVI
BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BssHII BssSI BstEII BstYI BtgI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI HaeII Hin4I HincII MscI
MslI MspA1I NspI PfoI PshAI PstI PvuII SacI
SfcI SmaI SmlI SphI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DrdI EagI EciI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmnI ZraI