Restriction map for
pF1KE2567
(Linear) (Six-base) MAPSORT of: KIEE2567.seq Check: 4371 from: 1 to: 2229
KIEE2567.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 294 2229
Size: 294 1935
AccI GT'mk_AC
Cuts at: 0 2225 2229
Size: 2225 4
Acc65I G'GTAC_C
Cuts at: 0 771 2229
Size: 771 1458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1041 1869 2050 2229
Size: 88 953 828 181 179
Fragments arranged by size:
953 828 181 179 88
AflIII A'CryG_T
Cuts at: 0 1265 2229
Size: 1265 964
AhdI GACnn_n'nnGTC
Cuts at: 0 178 2229
Size: 178 2051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1549 2229
Size: 1549 680
AlwNI CAG_nnn'CTG
Cuts at: 0 384 2229
Size: 384 1845
ApaI G_GGCC'C
Cuts at: 0 950 2229
Size: 950 1279
ApoI r'AATT_y
Cuts at: 0 91 2229
Size: 91 2138
AvaI C'yCGr_G
Cuts at: 0 561 943 2190 2229
Size: 561 382 1247 39
Fragments arranged by size:
1247 561 382 39
BanI G'GyrC_C
Cuts at: 0 17 771 1599 1771 2229
Size: 17 754 828 172 458
Fragments arranged by size:
828 754 458 172 17
BanII G_rGCy'C
Cuts at: 0 209 477 561 740 866 950 1024 1069
Size: 209 268 84 179 126 84 74 45
Cuts at: 1069 2229
Size: 1160
Fragments arranged by size:
1160 268 209 179 126 84 84 74
45
BbeI G_GCGC'C
Cuts at: 0 1603 1775 2229
Size: 1603 172 454
Fragments arranged by size:
1603 454 172
BbsI GAAGACnn'nnnn_
Cuts at: 0 163 695 2229
Size: 163 532 1534
Fragments arranged by size:
1534 532 163
BbvCI CC'TCA_GC
Cuts at: 0 1635 2229
Size: 1635 594
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 563 597 2229
Size: 563 34 1632
Fragments arranged by size:
1632 563 34
BfrBI ATG'CAT
Cuts at: 0 222 2229
Size: 222 2007
BglI GCCn_nnn'nGGC
Cuts at: 0 823 2229
Size: 823 1406
BglII A'GATC_T
Cuts at: 0 68 2229
Size: 68 2161
BlpI GC'TnA_GC
Cuts at: 0 491 2229
Size: 491 1738
Bme1580I G_kGCm'C
Cuts at: 0 410 950 1103 2229
Size: 410 540 153 1126
Fragments arranged by size:
1126 540 410 153
BmgBI CAC'GTC
Cuts at: 0 1052 1452 1974 2229
Size: 1052 400 522 255
Fragments arranged by size:
1052 522 400 255
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 593 2154 2229
Size: 593 1561 75
Fragments arranged by size:
1561 593 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 295 361 491 2112 2145 2229
Size: 16 279 66 130 1621 33 84
Fragments arranged by size:
1621 279 130 84 66 33 16
Bpu10I CC'TnA_GC
Cuts at: 0 1635 2229
Size: 1635 594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 2229
Size: 109 2120
BsaI GGTCTCn'nnnn_
Cuts at: 0 1082 2229
Size: 1082 1147
BsaAI yAC'GTr
Cuts at: 0 1266 2229
Size: 1266 963
BsaBI GATnn'nnATC
Cuts at: 0 704 2229
Size: 704 1525
BsaHI Gr'CG_yC
Cuts at: 0 1474 1600 1673 1772 2098 2229
Size: 1474 126 73 99 326 131
Fragments arranged by size:
1474 326 131 126 99 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1147 1177 1873 1903 2229
Size: 1147 30 696 30 326
Fragments arranged by size:
1147 696 326 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 622 906 1032 2229
Size: 619 3 284 126 1197
Fragments arranged by size:
1197 619 284 126 3
BseYI C'CCAG_C
Cuts at: 0 423 742 949 1102 1228 1484 1641 1844
Size: 423 319 207 153 126 256 157 203
Cuts at: 1844 2229
Size: 385
Fragments arranged by size:
423 385 319 256 207 203 157 153
126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1954 1984 2229
Size: 1336 618 30 245
Fragments arranged by size:
1336 618 245 30
BsiEI CG_ry'CG
Cuts at: 0 78 616 1940 2229
Size: 78 538 1324 289
Fragments arranged by size:
1324 538 289 78
BsiHKAI G_wGCw'C
Cuts at: 0 311 866 2065 2229
Size: 311 555 1199 164
Fragments arranged by size:
1199 555 311 164
BsiWI C'GTAC_G
Cuts at: 0 200 2102 2229
Size: 200 1902 127
Fragments arranged by size:
1902 200 127
BsmI GAATG_Cn'
Cuts at: 0 782 2229
Size: 782 1447
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1218 2229
Size: 1218 1011
Bsp1286I G_dGCh'C
Cuts at: 0 209 311 410 477 561 740 866 950
Size: 209 102 99 67 84 179 126 84
Cuts at: 950 1024 1069 1103 2065 2229
Size: 74 45 34 962 164
Fragments arranged by size:
962 209 179 164 126 102 99 84
84 74 67 45 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 324 1733 1859 2087 2229
Size: 294 30 1409 126 228 142
Fragments arranged by size:
1409 294 228 142 126 30
BsrBI CCG'CTC
Cuts at: 0 30 254 2229
Size: 30 224 1975
Fragments arranged by size:
1975 224 30
BsrFI r'CCGG_y
Cuts at: 0 1248 1290 2229
Size: 1248 42 939
Fragments arranged by size:
1248 939 42
BsrGI T'GTAC_A
Cuts at: 0 1415 2229
Size: 1415 814
BssSI C'ACGA_G
Cuts at: 0 598 2008 2229
Size: 598 1410 221
Fragments arranged by size:
1410 598 221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 2229
Size: 306 1923
BstEII G'GTnAC_C
Cuts at: 0 336 1386 2229
Size: 336 1050 843
Fragments arranged by size:
1050 843 336
BstYI r'GATC_y
Cuts at: 0 68 1964 2229
Size: 68 1896 265
Fragments arranged by size:
1896 265 68
Bsu36I CC'TnA_GG
Cuts at: 0 1015 2229
Size: 1015 1214
BtgI C'CryG_G
Cuts at: 0 341 998 1333 1365 1478 2229
Size: 341 657 335 32 113 751
Fragments arranged by size:
751 657 341 335 113 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1715 2229
Size: 1715 514
BtsI GCAGTG_nn'
Cuts at: 0 450 1911 2229
Size: 450 1461 318
Fragments arranged by size:
1461 450 318
EaeI y'GGCC_r
Cuts at: 0 39 146 956 1542 1658 2229
Size: 39 107 810 586 116 571
Fragments arranged by size:
810 586 571 116 107 39
EarI CTCTTCn'nnn_
Cuts at: 0 1221 2229
Size: 1221 1008
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 247 2229
Size: 247 1982
EcoICRI GAG'CTC
Cuts at: 0 864 2229
Size: 864 1365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 88 295 361 491 1041 1869 2050
Size: 16 72 207 66 130 550 828 181
Cuts at: 2050 2112 2145 2229
Size: 62 33 84
Fragments arranged by size:
828 550 207 181 130 84 72 66
62 33 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 1855 2229
Size: 286 1569 374
Fragments arranged by size:
1569 374 286
EcoO109I rG'GnC_Cy
Cuts at: 0 670 798 1590 1722 1821 1848 2229
Size: 670 128 792 132 99 27 381
Fragments arranged by size:
792 670 381 132 128 99 27
EcoRI G'AATT_C
Cuts at: 0 91 2229
Size: 91 2138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1701 2229
Size: 1701 528
HaeII r_GCGC'y
Cuts at: 0 1603 1775 2229
Size: 1603 172 454
Fragments arranged by size:
1603 454 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 164 196 715 747 1873 1905 2229
Size: 164 32 519 32 1126 32 324
Fragments arranged by size:
1126 519 324 164 32 32 32
KasI G'GCGC_C
Cuts at: 0 1599 1771 2229
Size: 1599 172 458
Fragments arranged by size:
1599 458 172
KpnI G_GTAC'C
Cuts at: 0 775 2229
Size: 775 1454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1920 2229
Size: 1920 309
MscI TGG'CCA
Cuts at: 0 148 2229
Size: 148 2081
MslI CAynn'nnrTG
Cuts at: 0 217 2229
Size: 217 2012
MspA1I CmG'CkG
Cuts at: 0 384 495 846 1335 1350 1397 1778 1869
Size: 384 111 351 489 15 47 381 91
Cuts at: 1869 1998 2229
Size: 129 231
Fragments arranged by size:
489 384 381 351 231 129 111 91
47 15
NaeI GCC'GGC
Cuts at: 0 1250 2229
Size: 1250 979
NarI GG'CG_CC
Cuts at: 0 1600 1772 2229
Size: 1600 172 457
Fragments arranged by size:
1600 457 172
NcoI C'CATG_G
Cuts at: 0 1365 2229
Size: 1365 864
NgoMIV G'CCGG_C
Cuts at: 0 1248 2229
Size: 1248 981
NsiI A_TGCA'T
Cuts at: 0 224 2229
Size: 224 2005
NspI r_CATG'y
Cuts at: 0 1414 2229
Size: 1414 815
PasI CC'CwG_GG
Cuts at: 0 9 1237 1905 2229
Size: 9 1228 668 324
Fragments arranged by size:
1228 668 324 9
PmlI CAC'GTG
Cuts at: 0 1266 2229
Size: 1266 963
PpuMI rG'GwC_Cy
Cuts at: 0 670 798 1590 1722 1848 2229
Size: 670 128 792 132 126 381
Fragments arranged by size:
792 670 381 132 128 126
PshAI GACnn'nnGTC
Cuts at: 0 1741 2229
Size: 1741 488
PspOMI G'GGCC_C
Cuts at: 0 946 2229
Size: 946 1283
PstI C_TGCA'G
Cuts at: 0 1699 1730 1874 2229
Size: 1699 31 144 355
Fragments arranged by size:
1699 355 144 31
PvuII CAG'CTG
Cuts at: 0 495 1350 1778 1869 1998 2229
Size: 495 855 428 91 129 231
Fragments arranged by size:
855 495 428 231 129 91
SacI G_AGCT'C
Cuts at: 0 866 2229
Size: 866 1363
SacII CC_GC'GG
Cuts at: 0 1336 2229
Size: 1336 893
SexAI A'CCwGG_T
Cuts at: 0 800 2229
Size: 800 1429
SfcI C'TryA_G
Cuts at: 0 1695 1726 1870 2229
Size: 1695 31 144 359
Fragments arranged by size:
1695 359 144 31
SfoI GGC'GCC
Cuts at: 0 1601 1773 2229
Size: 1601 172 456
Fragments arranged by size:
1601 456 172
SmaI CCC'GGG
Cuts at: 0 945 2229
Size: 945 1284
SmlI C'TyrA_G
Cuts at: 0 124 2229
Size: 124 2105
StyI C'CwwG_G
Cuts at: 0 821 834 857 1365 1676 2126 2229
Size: 821 13 23 508 311 450 103
Fragments arranged by size:
821 508 450 311 103 23 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 630 2229
Size: 630 1599
TatI w'GTAC_w
Cuts at: 0 1415 2229
Size: 1415 814
Tth111I GACn'n_nGTC
Cuts at: 0 1006 1032 2229
Size: 1006 26 1197
Fragments arranged by size:
1197 1006 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1476 1590 2229
Size: 1476 114 639
Fragments arranged by size:
1476 639 114
XmaI C'CCGG_G
Cuts at: 0 943 2229
Size: 943 1286
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII
BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SexAI SfcI SfoI
SmaI SmlI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BmrI BmtI BsaWI BspEI BspHI BsrDI BssHII BstBI
BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EagI
EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI
MluI NdeI NheI NotI NruI PacI PciI PflMI
PfoI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI XmnI ZraI