Restriction map for
pF1KE2564
(Linear) (Six-base) MAPSORT of: KIEE2564.seq Check: 856 from: 1 to: 1824
KIEE2564.orf
With 182 enzymes: SgfI *
March 13, 2015 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1102 1824
Size: 1102 722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1323 1746 1824
Size: 553 770 423 78
Fragments arranged by size:
770 553 423 78
AleI CACnn'nnGTG
Cuts at: 0 677 1447 1612 1751 1824
Size: 677 770 165 139 73
Fragments arranged by size:
770 677 165 139 73
AlwNI CAG_nnn'CTG
Cuts at: 0 17 398 1048 1824
Size: 17 381 650 776
Fragments arranged by size:
776 650 381 17
ApaI G_GGCC'C
Cuts at: 0 97 881 1824
Size: 97 784 943
Fragments arranged by size:
943 784 97
ApaLI G'TGCA_C
Cuts at: 0 1735 1824
Size: 1735 89
AvaI C'yCGr_G
Cuts at: 0 377 623 1824
Size: 377 246 1201
Fragments arranged by size:
1201 377 246
BamHI G'GATC_C
Cuts at: 0 812 1824
Size: 812 1012
BanI G'GyrC_C
Cuts at: 0 199 499 1081 1355 1441 1824
Size: 199 300 582 274 86 383
Fragments arranged by size:
582 383 300 274 199 86
BanII G_rGCy'C
Cuts at: 0 31 97 707 881 1824
Size: 31 66 610 174 943
Fragments arranged by size:
943 610 174 66 31
BbeI G_GCGC'C
Cuts at: 0 503 1359 1824
Size: 503 856 465
Fragments arranged by size:
856 503 465
BbsI GAAGACnn'nnnn_
Cuts at: 0 1205 1490 1824
Size: 1205 285 334
Fragments arranged by size:
1205 334 285
BbvCI CC'TCA_GC
Cuts at: 0 743 1454 1824
Size: 743 711 370
Fragments arranged by size:
743 711 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 431 1824
Size: 397 34 1393
Fragments arranged by size:
1393 397 34
BglI GCCn_nnn'nGGC
Cuts at: 0 449 934 1824
Size: 449 485 890
Fragments arranged by size:
890 485 449
BglII A'GATC_T
Cuts at: 0 1152 1824
Size: 1152 672
BlpI GC'TnA_GC
Cuts at: 0 1254 1263 1824
Size: 1254 9 561
Fragments arranged by size:
1254 561 9
Bme1580I G_kGCm'C
Cuts at: 0 97 202 553 724 881 1074 1739 1824
Size: 97 105 351 171 157 193 665 85
Fragments arranged by size:
665 351 193 171 157 105 97 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 806 921 958 1002 1824
Size: 806 115 37 44 822
Fragments arranged by size:
822 806 115 44 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 659 1524 1824
Size: 659 865 300
Fragments arranged by size:
865 659 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 943 1145 1621 1824
Size: 530 413 202 476 203
Fragments arranged by size:
530 476 413 203 202
Bpu10I CC'TnA_GC
Cuts at: 0 743 1454 1824
Size: 743 711 370
Fragments arranged by size:
743 711 370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1824
Size: 354 1470
BsaI GGTCTCn'nnnn_
Cuts at: 0 80 799 976 1177 1824
Size: 80 719 177 201 647
Fragments arranged by size:
719 647 201 177 80
BsaBI GATnn'nnATC
Cuts at: 0 1603 1824
Size: 1603 221
BsaHI Gr'CG_yC
Cuts at: 0 500 1290 1356 1824
Size: 500 790 66 468
Fragments arranged by size:
790 500 468 66
BsaWI w'CCGG_w
Cuts at: 0 776 1824
Size: 776 1048
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 973 1003 1824
Size: 973 30 821
Fragments arranged by size:
973 821 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1296 1824
Size: 1296 528
BseYI C'CCAG_C
Cuts at: 0 255 383 418 606 887 1351 1562 1824
Size: 255 128 35 188 281 464 211 262
Fragments arranged by size:
464 281 262 255 211 188 128 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1023 1147 1198 1824
Size: 78 945 124 51 626
Fragments arranged by size:
945 626 124 78 51
BsiHKAI G_wGCw'C
Cuts at: 0 624 707 1739 1824
Size: 624 83 1032 85
Fragments arranged by size:
1032 624 85 83
Bsp1286I G_dGCh'C
Cuts at: 0 31 97 202 553 624 707 724 881
Size: 31 66 105 351 71 83 17 157
Cuts at: 881 1074 1739 1824
Size: 193 665 85
Fragments arranged by size:
665 351 193 157 105 85 83 71
66 31 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 1102 1824
Size: 584 518 722
Fragments arranged by size:
722 584 518
BsrBI CCG'CTC
Cuts at: 0 1262 1824
Size: 1262 562
BsrDI GCAATG_nn'
Cuts at: 0 1700 1824
Size: 1700 124
BsrFI r'CCGG_y
Cuts at: 0 1202 1286 1574 1824
Size: 1202 84 288 250
Fragments arranged by size:
1202 288 250 84
BssSI C'ACGA_G
Cuts at: 0 1375 1824
Size: 1375 449
BstAPI GCAn_nnn'nTGC
Cuts at: 0 65 206 1824
Size: 65 141 1618
Fragments arranged by size:
1618 141 65
BstEII G'GTnAC_C
Cuts at: 0 1282 1824
Size: 1282 542
BstYI r'GATC_y
Cuts at: 0 812 1152 1313 1566 1824
Size: 812 340 161 253 258
Fragments arranged by size:
812 340 258 253 161
BtgI C'CryG_G
Cuts at: 0 89 929 969 1383 1824
Size: 89 840 40 414 441
Fragments arranged by size:
840 441 414 89 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 224 1824
Size: 224 1600
BtsI GCAGTG_nn'
Cuts at: 0 28 348 1118 1824
Size: 28 320 770 706
Fragments arranged by size:
770 706 320 28
DraIII CAC_nnn'GTG
Cuts at: 0 1493 1824
Size: 1493 331
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 1053 1824
Size: 466 587 771
Fragments arranged by size:
771 587 466
EaeI y'GGCC_r
Cuts at: 0 1247 1800 1824
Size: 1247 553 24
Fragments arranged by size:
1247 553 24
EarI CTCTTCn'nnn_
Cuts at: 0 1229 1763 1824
Size: 1229 534 61
Fragments arranged by size:
1229 534 61
EcoICRI GAG'CTC
Cuts at: 0 705 1824
Size: 705 1119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 553 943 1145 1323 1621 1746 1824
Size: 530 23 390 202 178 298 125 78
Fragments arranged by size:
530 390 298 202 178 125 78 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 308 1098 1824
Size: 308 790 726
Fragments arranged by size:
790 726 308
EcoO109I rG'GnC_Cy
Cuts at: 0 94 573 871 878 891 917 1109 1824
Size: 94 479 298 7 13 26 192 715
Fragments arranged by size:
715 479 298 192 94 26 13 7
EcoRV GAT'ATC
Cuts at: 0 1425 1824
Size: 1425 399
HaeII r_GCGC'y
Cuts at: 0 444 503 644 1359 1824
Size: 444 59 141 715 465
Fragments arranged by size:
715 465 444 141 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 1132 1164 1712 1744 1824
Size: 689 32 411 32 548 32 80
Fragments arranged by size:
689 548 411 80 32 32 32
KasI G'GCGC_C
Cuts at: 0 499 1355 1824
Size: 499 856 469
Fragments arranged by size:
856 499 469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1824
Size: 840 984
MscI TGG'CCA
Cuts at: 0 1249 1802 1824
Size: 1249 553 22
Fragments arranged by size:
1249 553 22
MslI CAynn'nnrTG
Cuts at: 0 677 1447 1552 1612 1751 1824
Size: 677 770 105 60 139 73
Fragments arranged by size:
770 677 139 105 73 60
MspA1I CmG'CkG
Cuts at: 0 742 1824
Size: 742 1082
NarI GG'CG_CC
Cuts at: 0 500 1356 1824
Size: 500 856 468
Fragments arranged by size:
856 500 468
NcoI C'CATG_G
Cuts at: 0 929 1383 1824
Size: 929 454 441
Fragments arranged by size:
929 454 441
NdeI CA'TA_TG
Cuts at: 0 1214 1824
Size: 1214 610
NspI r_CATG'y
Cuts at: 0 583 1824
Size: 583 1241
PasI CC'CwG_GG
Cuts at: 0 32 1824
Size: 32 1792
PflMI CCAn_nnn'nTGG
Cuts at: 0 278 545 1323 1401 1824
Size: 278 267 778 78 423
Fragments arranged by size:
778 423 278 267 78
PpuMI rG'GwC_Cy
Cuts at: 0 573 871 917 1109 1824
Size: 573 298 46 192 715
Fragments arranged by size:
715 573 298 192 46
PspOMI G'GGCC_C
Cuts at: 0 93 877 1824
Size: 93 784 947
Fragments arranged by size:
947 784 93
PstI C_TGCA'G
Cuts at: 0 230 343 980 1824
Size: 230 113 637 844
Fragments arranged by size:
844 637 230 113
PvuII CAG'CTG
Cuts at: 0 742 1824
Size: 742 1082
SacI G_AGCT'C
Cuts at: 0 707 1824
Size: 707 1117
SanDI GG'GwC_CC
Cuts at: 0 917 1109 1824
Size: 917 192 715
Fragments arranged by size:
917 715 192
SbfI CC_TGCA'GG
Cuts at: 0 980 1824
Size: 980 844
SexAI A'CCwGG_T
Cuts at: 0 985 1824
Size: 985 839
SfcI C'TryA_G
Cuts at: 0 226 339 939 976 1824
Size: 226 113 600 37 848
Fragments arranged by size:
848 600 226 113 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 934 1824
Size: 934 890
SfoI GGC'GCC
Cuts at: 0 501 1357 1824
Size: 501 856 467
Fragments arranged by size:
856 501 467
SgrAI Cr'CCGG_yG
Cuts at: 0 1286 1824
Size: 1286 538
SmaI CCC'GGG
Cuts at: 0 379 1824
Size: 379 1445
SmlI C'TyrA_G
Cuts at: 0 333 623 1824
Size: 333 290 1201
Fragments arranged by size:
1201 333 290
SphI G_CATG'C
Cuts at: 0 583 1824
Size: 583 1241
StyI C'CwwG_G
Cuts at: 0 929 1346 1383 1445 1824
Size: 929 417 37 62 379
Fragments arranged by size:
929 417 379 62 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 652 1099 1356 1722 1824
Size: 364 288 447 257 366 102
Fragments arranged by size:
447 366 364 288 257 102
TatI w'GTAC_w
Cuts at: 0 1220 1824
Size: 1220 604
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 273 305 1824
Size: 273 32 1519
Fragments arranged by size:
1519 273 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 445 1824
Size: 445 1379
XhoI C'TCGA_G
Cuts at: 0 623 1824
Size: 623 1201
XmaI C'CCGG_G
Cuts at: 0 377 1824
Size: 377 1447
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI
MmeI MscI MslI MspA1I NarI NcoI NdeI NspI
PasI PflMI PpuMI PspOMI PstI PvuII SacI SanDI
SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BciVI BclI BfrBI BmgBI BmtI BsaAI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstXI BstZ17I Bsu36I ClaI CspCI DraI EagI EciI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
ScaI SgfI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XmnI ZraI