(Linear) (Six-base) MAPSORT of: KIEE2560.seq  Check: 5018  from: 1  to: 627



KIEE2560.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0     599     627 
   Size:        599      28
 
AclI AA'CG_TT

Cuts at:      0     264     627 
   Size:        264     363
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     627 
   Size:        527     100
 
AflIII A'CryG_T

Cuts at:      0     192     351     627 
   Size:        192     159     276

  Fragments arranged by size: 

                 276     192     159
 
ApoI r'AATT_y

Cuts at:      0     276     627 
   Size:        276     351
 
AvaI C'yCGr_G

Cuts at:      0      69      94     627 
   Size:         69      25     533

  Fragments arranged by size: 

                 533      69      25
 
BanI G'GyrC_C

Cuts at:      0      48     627 
   Size:         48     579
 
BbeI G_GCGC'C

Cuts at:      0      52     627 
   Size:         52     575
 
BglII A'GATC_T

Cuts at:      0     174     627 
   Size:        174     453
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      26     387     627 
   Size:         26     361     240

  Fragments arranged by size: 

                 361     240      26
 
BsaAI yAC'GTr

Cuts at:      0     354     627 
   Size:        354     273
 
BsaHI Gr'CG_yC

Cuts at:      0      49     627 
   Size:         49     578
 
BsaWI w'CCGG_w

Cuts at:      0      60     627 
   Size:         60     567
 
BsmI GAATG_Cn'

Cuts at:      0     156     627 
   Size:        156     471
 
BspEI T'CCGG_A

Cuts at:      0      60     627 
   Size:         60     567
 
BstYI r'GATC_y

Cuts at:      0     174     627 
   Size:        174     453
 
DraI TTT'AAA

Cuts at:      0     237     560     627 
   Size:        237     323      67

  Fragments arranged by size: 

                 323     237      67
 
EarI CTCTTCn'nnn_

Cuts at:      0     569     627 
   Size:        569      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     627 
   Size:        527     100
 
EcoRV GAT'ATC

Cuts at:      0     513     627 
   Size:        513     114
 
HaeII r_GCGC'y

Cuts at:      0      52     627 
   Size:         52     575
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     627 
   Size:        118      32     477

  Fragments arranged by size: 

                 477     118      32
 
HindIII A'AGCT_T

Cuts at:      0     100     627 
   Size:        100     527
 
KasI G'GCGC_C

Cuts at:      0      48     627 
   Size:         48     579
 
NarI GG'CG_CC

Cuts at:      0      49     627 
   Size:         49     578
 
NspI r_CATG'y

Cuts at:      0     196     627 
   Size:        196     431
 
PciI A'CATG_T

Cuts at:      0     192     627 
   Size:        192     435
 
PmlI CAC'GTG

Cuts at:      0     354     627 
   Size:        354     273
 
PsiI TTA'TAA

Cuts at:      0     409     500     627 
   Size:        409      91     127

  Fragments arranged by size: 

                 409     127      91
 
ScaI AGT'ACT

Cuts at:      0     258     627 
   Size:        258     369
 
SfcI C'TryA_G

Cuts at:      0     178     627 
   Size:        178     449
 
SfoI GGC'GCC

Cuts at:      0      50     627 
   Size:         50     577
 
SmaI CCC'GGG

Cuts at:      0      71     627 
   Size:         71     556
 
SmlI C'TyrA_G

Cuts at:      0      94     627 
   Size:         94     533
 
TatI w'GTAC_w

Cuts at:      0     256     317     627 
   Size:        256      61     310

  Fragments arranged by size: 

                 310     256      61
 
XhoI C'TCGA_G

Cuts at:      0      94     627 
   Size:         94     533
 
XmaI C'CCGG_G

Cuts at:      0      69     627 
   Size:         69     558
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     ApoI     AvaI     BanI     BbeI
    BglII     BplI    BsaAI    BsaHI    BsaWI     BsmI    BspEI    BstYI
     DraI     EarI  Eco57MI    EcoRV    HaeII    Hin4I  HindIII     KasI
     NarI     NspI     PciI     PmlI     PsiI     ScaI     SfcI     SfoI
     SmaI     SmlI     TatI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI