Restriction map for
pF1KE2555
(Linear) (Six-base) MAPSORT of: KIEE2555.seq Check: 9470 from: 1 to: 1368
KIEE2555.orf
With 182 enzymes: SgfI *
December 15, 2014 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1251 1368
Size: 1251 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 667 827 1368
Size: 402 265 160 541
Fragments arranged by size:
541 402 265 160
AfeI AGC'GCT
Cuts at: 0 344 881 1368
Size: 344 537 487
Fragments arranged by size:
537 487 344
AlwNI CAG_nnn'CTG
Cuts at: 0 504 702 1266 1368
Size: 504 198 564 102
Fragments arranged by size:
564 504 198 102
AvaI C'yCGr_G
Cuts at: 0 181 1368
Size: 181 1187
BamHI G'GATC_C
Cuts at: 0 155 1368
Size: 155 1213
BanI G'GyrC_C
Cuts at: 0 70 457 1251 1291 1368
Size: 70 387 794 40 77
Fragments arranged by size:
794 387 77 70 40
BanII G_rGCy'C
Cuts at: 0 367 722 824 1368
Size: 367 355 102 544
Fragments arranged by size:
544 367 355 102
BbeI G_GCGC'C
Cuts at: 0 1255 1295 1368
Size: 1255 40 73
Fragments arranged by size:
1255 73 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 840 849 891 912 1368
Size: 840 9 42 21 456
Fragments arranged by size:
840 456 42 21 9
BbvCI CC'TCA_GC
Cuts at: 0 490 1368
Size: 490 878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 426 460 553 587 1368
Size: 426 34 93 34 781
Fragments arranged by size:
781 426 93 34 34
BglII A'GATC_T
Cuts at: 0 866 1368
Size: 866 502
BlpI GC'TnA_GC
Cuts at: 0 716 1368
Size: 716 652
Bme1580I G_kGCm'C
Cuts at: 0 73 460 1118 1142 1368
Size: 73 387 658 24 226
Fragments arranged by size:
658 387 226 73 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 743 1368
Size: 743 625
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 656 1357 1368
Size: 656 701 11
Fragments arranged by size:
701 656 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 724 811 1368
Size: 709 15 87 557
Fragments arranged by size:
709 557 87 15
Bpu10I CC'TnA_GC
Cuts at: 0 160 490 631 695 1368
Size: 160 330 141 64 673
Fragments arranged by size:
673 330 160 141 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 700 1368
Size: 559 141 668
Fragments arranged by size:
668 559 141
BsaAI yAC'GTr
Cuts at: 0 1137 1368
Size: 1137 231
BsaHI Gr'CG_yC
Cuts at: 0 14 560 1252 1292 1368
Size: 14 546 692 40 76
Fragments arranged by size:
692 546 76 40 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 241 712 994 1368
Size: 23 218 471 282 374
Fragments arranged by size:
471 374 282 218 23
BseYI C'CCAG_C
Cuts at: 0 66 224 453 508 1026 1368
Size: 66 158 229 55 518 342
Fragments arranged by size:
518 342 229 158 66 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 1368
Size: 443 925
BsiHKAI G_wGCw'C
Cuts at: 0 166 722 1368
Size: 166 556 646
Fragments arranged by size:
646 556 166
BsmI GAATG_Cn'
Cuts at: 0 293 1368
Size: 293 1075
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5 277 1368
Size: 5 272 1091
Fragments arranged by size:
1091 272 5
Bsp1286I G_dGCh'C
Cuts at: 0 73 166 367 460 722 824 1118 1142
Size: 73 93 201 93 262 102 294 24
Cuts at: 1142 1368
Size: 226
Fragments arranged by size:
294 262 226 201 102 93 93 73
24
BspHI T'CATG_A
Cuts at: 0 1298 1368
Size: 1298 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1251 1368
Size: 1251 117
BsrFI r'CCGG_y
Cuts at: 0 136 1073 1288 1368
Size: 136 937 215 80
Fragments arranged by size:
937 215 136 80
BssSI C'ACGA_G
Cuts at: 0 1273 1368
Size: 1273 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1266 1368
Size: 1266 102
BstEII G'GTnAC_C
Cuts at: 0 942 1368
Size: 942 426
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 555 1248 1368
Size: 390 165 693 120
Fragments arranged by size:
693 390 165 120
BstYI r'GATC_y
Cuts at: 0 155 512 780 866 1368
Size: 155 357 268 86 502
Fragments arranged by size:
502 357 268 155 86
BtgI C'CryG_G
Cuts at: 0 19 389 542 938 1368
Size: 19 370 153 396 430
Fragments arranged by size:
430 396 370 153 19
DraIII CAC_nnn'GTG
Cuts at: 0 1244 1368
Size: 1244 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 219 1368
Size: 219 1149
EaeI y'GGCC_r
Cuts at: 0 128 311 495 545 668 1057 1286 1368
Size: 128 183 184 50 123 389 229 82
Fragments arranged by size:
389 229 184 183 128 123 82 50
EarI CTCTTCn'nnn_
Cuts at: 0 247 448 490 1012 1126 1171 1357 1368
Size: 247 201 42 522 114 45 186 11
Fragments arranged by size:
522 247 201 186 114 45 42 11
EcoICRI GAG'CTC
Cuts at: 0 720 1368
Size: 720 648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 667 709 724 811 827 1368
Size: 402 265 42 15 87 16 541
Fragments arranged by size:
541 402 265 87 42 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 793 1354 1368
Size: 793 561 14
Fragments arranged by size:
793 561 14
EcoO109I rG'GnC_Cy
Cuts at: 0 178 501 1148 1368
Size: 178 323 647 220
Fragments arranged by size:
647 323 220 178
FspI TGC'GCA
Cuts at: 0 292 1368
Size: 292 1076
FspAI rTGC'GCAy
Cuts at: 0 292 1368
Size: 292 1076
HaeII r_GCGC'y
Cuts at: 0 346 883 1255 1295 1363 1368
Size: 346 537 372 40 68 5
Fragments arranged by size:
537 372 346 68 40 5
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 790 797 822 1368
Size: 765 25 7 25 546
Fragments arranged by size:
765 546 25 25 7
KasI G'GCGC_C
Cuts at: 0 1251 1291 1368
Size: 1251 40 77
Fragments arranged by size:
1251 77 40
MfeI C'AATT_G
Cuts at: 0 204 1368
Size: 204 1164
MscI TGG'CCA
Cuts at: 0 313 547 670 1059 1368
Size: 313 234 123 389 309
Fragments arranged by size:
389 313 309 234 123
MslI CAynn'nnrTG
Cuts at: 0 388 610 937 1195 1368
Size: 388 222 327 258 173
Fragments arranged by size:
388 327 258 222 173
MspA1I CmG'CkG
Cuts at: 0 21 228 453 494 1368
Size: 21 207 225 41 874
Fragments arranged by size:
874 225 207 41 21
NaeI GCC'GGC
Cuts at: 0 138 1075 1290 1368
Size: 138 937 215 78
Fragments arranged by size:
937 215 138 78
NarI GG'CG_CC
Cuts at: 0 1252 1292 1368
Size: 1252 40 76
Fragments arranged by size:
1252 76 40
NcoI C'CATG_G
Cuts at: 0 389 542 938 1368
Size: 389 153 396 430
Fragments arranged by size:
430 396 389 153
NgoMIV G'CCGG_C
Cuts at: 0 136 1073 1288 1368
Size: 136 937 215 80
Fragments arranged by size:
937 215 136 80
PasI CC'CwG_GG
Cuts at: 0 576 1317 1368
Size: 576 741 51
Fragments arranged by size:
741 576 51
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 1368
Size: 504 864
PfoI T'CCnGG_A
Cuts at: 0 278 1368
Size: 278 1090
PmlI CAC'GTG
Cuts at: 0 1137 1368
Size: 1137 231
PpuMI rG'GwC_Cy
Cuts at: 0 178 1148 1368
Size: 178 970 220
Fragments arranged by size:
970 220 178
PvuII CAG'CTG
Cuts at: 0 228 453 1368
Size: 228 225 915
Fragments arranged by size:
915 228 225
SacI G_AGCT'C
Cuts at: 0 722 1368
Size: 722 646
SacII CC_GC'GG
Cuts at: 0 22 1368
Size: 22 1346
SanDI GG'GwC_CC
Cuts at: 0 178 1368
Size: 178 1190
SapI GCTCTTCn'nnn_
Cuts at: 0 247 1368
Size: 247 1121
SexAI A'CCwGG_T
Cuts at: 0 35 1226 1368
Size: 35 1191 142
Fragments arranged by size:
1191 142 35
SfcI C'TryA_G
Cuts at: 0 430 474 1368
Size: 430 44 894
Fragments arranged by size:
894 430 44
SfoI GGC'GCC
Cuts at: 0 1253 1293 1368
Size: 1253 40 75
Fragments arranged by size:
1253 75 40
StyI C'CwwG_G
Cuts at: 0 389 542 938 1368
Size: 389 153 396 430
Fragments arranged by size:
430 396 389 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 198 1368
Size: 198 1170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 63 95 1368
Size: 63 32 1273
Fragments arranged by size:
1273 63 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 505 936 1368
Size: 505 431 432
Fragments arranged by size:
505 432 431
XmnI GAAnn'nnTTC
Cuts at: 0 91 840 1368
Size: 91 749 528
Fragments arranged by size:
749 528 91
Enzymes that do cut:
AarI AcuI AfeI AlwNI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I KasI MfeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI
PvuII SacI SacII SanDI SapI SexAI SfcI SfoI
StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BglI BmgBI
BmtI BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BspEI
BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI EagI EciI EcoRI EcoRV
FalI FseI HincII HindIII HpaI KpnI MluI MmeI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SalI SbfI ScaI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI ZraI