Restriction map for
pF1KE2554
(Linear) (Six-base) MAPSORT of: KIEE2554.seq Check: 4043 from: 1 to: 1083
KIEE2554.orf
With 182 enzymes: SgfI *
December 15, 2014 12:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 792 954 1083
Size: 544 248 162 129
Fragments arranged by size:
544 248 162 129
AleI CACnn'nnGTG
Cuts at: 0 257 757 1083
Size: 257 500 326
Fragments arranged by size:
500 326 257
ApaLI G'TGCA_C
Cuts at: 0 727 750 856 1083
Size: 727 23 106 227
Fragments arranged by size:
727 227 106 23
ApoI r'AATT_y
Cuts at: 0 997 1083
Size: 997 86
BanI G'GyrC_C
Cuts at: 0 624 1083
Size: 624 459
BanII G_rGCy'C
Cuts at: 0 78 86 145 779 1083
Size: 78 8 59 634 304
Fragments arranged by size:
634 304 78 59 8
BbeI G_GCGC'C
Cuts at: 0 628 1083
Size: 628 455
BbsI GAAGACnn'nnnn_
Cuts at: 0 726 1083
Size: 726 357
BfrBI ATG'CAT
Cuts at: 0 268 1083
Size: 268 815
BglI GCCn_nnn'nGGC
Cuts at: 0 880 1083
Size: 880 203
Bme1580I G_kGCm'C
Cuts at: 0 731 754 860 1083
Size: 731 23 106 223
Fragments arranged by size:
731 223 106 23
BmgBI CAC'GTC
Cuts at: 0 732 1083
Size: 732 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 1083
Size: 415 668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1083
Size: 337 746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1083
Size: 829 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 757 1083
Size: 757 326
BsaHI Gr'CG_yC
Cuts at: 0 625 1083
Size: 625 458
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 58 135 1083
Size: 55 3 77 948
Fragments arranged by size:
948 77 55 3
BseYI C'CCAG_C
Cuts at: 0 802 1083
Size: 802 281
BsiHKAI G_wGCw'C
Cuts at: 0 86 145 731 754 779 860 1083
Size: 86 59 586 23 25 81 223
Fragments arranged by size:
586 223 86 81 59 25 23
BsiWI C'GTAC_G
Cuts at: 0 298 1083
Size: 298 785
Bsp1286I G_dGCh'C
Cuts at: 0 78 86 145 731 754 779 860 1083
Size: 78 8 59 586 23 25 81 223
Fragments arranged by size:
586 223 81 78 59 25 23 8
BsrDI GCAATG_nn'
Cuts at: 0 1052 1083
Size: 1052 31
BsrFI r'CCGG_y
Cuts at: 0 604 1083
Size: 604 479
BssSI C'ACGA_G
Cuts at: 0 859 1083
Size: 859 224
BstAPI GCAn_nnn'nTGC
Cuts at: 0 371 1083
Size: 371 712
BstEII G'GTnAC_C
Cuts at: 0 834 1083
Size: 834 249
Bsu36I CC'TnA_GG
Cuts at: 0 26 1083
Size: 26 1057
BtsI GCAGTG_nn'
Cuts at: 0 157 930 1083
Size: 157 773 153
Fragments arranged by size:
773 157 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 906 941 1083
Size: 906 35 142
Fragments arranged by size:
906 142 35
EaeI y'GGCC_r
Cuts at: 0 515 1058 1083
Size: 515 543 25
Fragments arranged by size:
543 515 25
EarI CTCTTCn'nnn_
Cuts at: 0 32 451 823 1083
Size: 32 419 372 260
Fragments arranged by size:
419 372 260 32
EcoICRI GAG'CTC
Cuts at: 0 84 143 777 1083
Size: 84 59 634 306
Fragments arranged by size:
634 306 84 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 544 792 954 1083
Size: 337 207 248 162 129
Fragments arranged by size:
337 248 207 162 129
EcoO109I rG'GnC_Cy
Cuts at: 0 191 1083
Size: 191 892
EcoRV GAT'ATC
Cuts at: 0 978 1083
Size: 978 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 306 1083
Size: 306 777
FspI TGC'GCA
Cuts at: 0 952 1083
Size: 952 131
HaeII r_GCGC'y
Cuts at: 0 628 1083
Size: 628 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 226 258 1083
Size: 178 32 16 32 825
Fragments arranged by size:
825 178 32 32 16
HincII GTy'rAC
Cuts at: 0 894 1083
Size: 894 189
KasI G'GCGC_C
Cuts at: 0 624 1083
Size: 624 459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1083
Size: 418 665
MslI CAynn'nnrTG
Cuts at: 0 257 269 757 1083
Size: 257 12 488 326
Fragments arranged by size:
488 326 257 12
MspA1I CmG'CkG
Cuts at: 0 341 743 1083
Size: 341 402 340
Fragments arranged by size:
402 341 340
NarI GG'CG_CC
Cuts at: 0 625 1083
Size: 625 458
NsiI A_TGCA'T
Cuts at: 0 270 1083
Size: 270 813
NspI r_CATG'y
Cuts at: 0 106 272 414 1083
Size: 106 166 142 669
Fragments arranged by size:
669 166 142 106
PfoI T'CCnGG_A
Cuts at: 0 32 136 1083
Size: 32 104 947
Fragments arranged by size:
947 104 32
PstI C_TGCA'G
Cuts at: 0 176 1083
Size: 176 907
PvuII CAG'CTG
Cuts at: 0 341 1083
Size: 341 742
SacI G_AGCT'C
Cuts at: 0 86 145 779 1083
Size: 86 59 634 304
Fragments arranged by size:
634 304 86 59
ScaI AGT'ACT
Cuts at: 0 379 1083
Size: 379 704
SexAI A'CCwGG_T
Cuts at: 0 830 1083
Size: 830 253
SfcI C'TryA_G
Cuts at: 0 172 1083
Size: 172 911
SfoI GGC'GCC
Cuts at: 0 626 1083
Size: 626 457
SmlI C'TyrA_G
Cuts at: 0 844 1083
Size: 844 239
SphI G_CATG'C
Cuts at: 0 106 1083
Size: 106 977
StuI AGG'CCT
Cuts at: 0 529 1083
Size: 529 554
TatI w'GTAC_w
Cuts at: 0 377 500 1083
Size: 377 123 583
Fragments arranged by size:
583 377 123
XmnI GAAnn'nnTTC
Cuts at: 0 529 565 1083
Size: 529 36 518
Fragments arranged by size:
529 518 36
Enzymes that do cut:
AcuI AleI ApaLI ApoI BanI BanII BbeI BbsI
BfrBI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaHI BseRI BseYI BsiHKAI BsiWI Bsp1286I BsrDI BsrFI
BssSI BstAPI BstEII Bsu36I BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII KasI MmeI MslI MspA1I NarI NsiI NspI
PfoI PstI PvuII SacI ScaI SexAI SfcI SfoI
SmlI SphI StuI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BglII BlpI BmtI BplI Bpu10I BsaAI BsaBI
BsaWI BsaXI BsgI BsiEI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BstBI BstXI BstYI BstZ17I
BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRI FseI FspAI HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI