(Linear) (Six-base) MAPSORT of: KIEE2552.seq  Check: 1599  from: 1  to: 501



KIEE2552.orf

 With 182 enzymes: SgfI * 

                        November 21, 2014 11:13  ..

AccI GT'mk_AC

Cuts at:      0     251     501 
   Size:        251     250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     289     501 
   Size:        171     118     212

  Fragments arranged by size: 

                 212     171     118
 
ApoI r'AATT_y

Cuts at:      0     130     501 
   Size:        130     371
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     201     501 
   Size:        201     300
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      25      59     501 
   Size:         25      34     442

  Fragments arranged by size: 

                 442      34      25
 
BglII A'GATC_T

Cuts at:      0     310     501 
   Size:        310     191
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     501 
   Size:        260     241
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     233     263     501 
   Size:        233      30     238

  Fragments arranged by size: 

                 238     233      30
 
BsrDI GCAATG_nn'

Cuts at:      0     239     501 
   Size:        239     262
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     114     501 
   Size:        114     387
 
BstYI r'GATC_y

Cuts at:      0     310     501 
   Size:        310     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     260     289     501 
   Size:        171      89      29     212

  Fragments arranged by size: 

                 212     171      89      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     233     265     501 
   Size:        233      32     236

  Fragments arranged by size: 

                 236     233      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     362     501 
   Size:        362     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     124     501 
   Size:        124     377
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111     501 
   Size:        111     390
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BbsI     BcgI    BglII     BpmI    BsaXI
    BsrDI    BstXI    BstYI  Eco57MI    Hin4I    PflMI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI