Restriction map for
pF1KE2534
(Linear) (Six-base) MAPSORT of: KIEE2534.seq Check: 6847 from: 1 to: 1032
KIEE2534.orf
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 804 1032
Size: 804 228
AccI GT'mk_AC
Cuts at: 0 419 1032
Size: 419 613
AlwNI CAG_nnn'CTG
Cuts at: 0 916 955 1032
Size: 916 39 77
Fragments arranged by size:
916 77 39
ApaI G_GGCC'C
Cuts at: 0 278 1032
Size: 278 754
BamHI G'GATC_C
Cuts at: 0 631 883 1032
Size: 631 252 149
Fragments arranged by size:
631 252 149
BanII G_rGCy'C
Cuts at: 0 278 851 1032
Size: 278 573 181
Fragments arranged by size:
573 278 181
BbvCI CC'TCA_GC
Cuts at: 0 12 1032
Size: 12 1020
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 990 1024 1032
Size: 990 34 8
Fragments arranged by size:
990 34 8
BclI T'GATC_A
Cuts at: 0 944 1032
Size: 944 88
BfrBI ATG'CAT
Cuts at: 0 313 1032
Size: 313 719
BglI GCCn_nnn'nGGC
Cuts at: 0 1021 1032
Size: 1021 11
BglII A'GATC_T
Cuts at: 0 381 1032
Size: 381 651
Bme1580I G_kGCm'C
Cuts at: 0 278 1032
Size: 278 754
BmrI ACTGGGnnnn_n'
Cuts at: 0 676 1032
Size: 676 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1032
Size: 432 600
Bpu10I CC'TnA_GC
Cuts at: 0 12 1032
Size: 12 1020
BsaWI w'CCGG_w
Cuts at: 0 403 1032
Size: 403 629
BseYI C'CCAG_C
Cuts at: 0 657 1032
Size: 657 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1032
Size: 782 250
BsiHKAI G_wGCw'C
Cuts at: 0 851 966 1032
Size: 851 115 66
Fragments arranged by size:
851 115 66
Bsp1286I G_dGCh'C
Cuts at: 0 278 851 966 1032
Size: 278 573 115 66
Fragments arranged by size:
573 278 115 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 804 1032
Size: 179 625 228
Fragments arranged by size:
625 228 179
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1032
Size: 468 564
BstYI r'GATC_y
Cuts at: 0 381 631 883 1032
Size: 381 250 252 149
Fragments arranged by size:
381 252 250 149
BstZ17I GTA'TAC
Cuts at: 0 420 1032
Size: 420 612
Bsu36I CC'TnA_GG
Cuts at: 0 506 1032
Size: 506 526
BtgI C'CryG_G
Cuts at: 0 467 1032
Size: 467 565
BtsI GCAGTG_nn'
Cuts at: 0 95 681 1032
Size: 95 586 351
Fragments arranged by size:
586 351 95
EaeI y'GGCC_r
Cuts at: 0 983 1013 1032
Size: 983 30 19
Fragments arranged by size:
983 30 19
EcoICRI GAG'CTC
Cuts at: 0 849 1032
Size: 849 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1032
Size: 432 600
EcoO109I rG'GnC_Cy
Cuts at: 0 4 51 274 1032
Size: 4 47 223 758
Fragments arranged by size:
758 223 47 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 585 616 617 648 1032
Size: 20 32 533 31 1 31 384
Fragments arranged by size:
533 384 32 31 31 20 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1032
Size: 485 547
MscI TGG'CCA
Cuts at: 0 985 1015 1032
Size: 985 30 17
Fragments arranged by size:
985 30 17
MslI CAynn'nnrTG
Cuts at: 0 868 1032
Size: 868 164
MspA1I CmG'CkG
Cuts at: 0 197 576 979 1032
Size: 197 379 403 53
Fragments arranged by size:
403 379 197 53
NsiI A_TGCA'T
Cuts at: 0 315 1032
Size: 315 717
NspI r_CATG'y
Cuts at: 0 37 1032
Size: 37 995
PasI CC'CwG_GG
Cuts at: 0 271 1032
Size: 271 761
PpuMI rG'GwC_Cy
Cuts at: 0 4 51 1032
Size: 4 47 981
Fragments arranged by size:
981 47 4
PshAI GACnn'nnGTC
Cuts at: 0 934 1032
Size: 934 98
PspOMI G'GGCC_C
Cuts at: 0 274 1032
Size: 274 758
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 508 540 1032
Size: 508 32 492
Fragments arranged by size:
508 492 32
PstI C_TGCA'G
Cuts at: 0 28 575 1032
Size: 28 547 457
Fragments arranged by size:
547 457 28
PvuII CAG'CTG
Cuts at: 0 197 576 979 1032
Size: 197 379 403 53
Fragments arranged by size:
403 379 197 53
SacI G_AGCT'C
Cuts at: 0 851 1032
Size: 851 181
SanDI GG'GwC_CC
Cuts at: 0 4 1032
Size: 4 1028
ScaI AGT'ACT
Cuts at: 0 805 1032
Size: 805 227
SfcI C'TryA_G
Cuts at: 0 24 571 1032
Size: 24 547 461
Fragments arranged by size:
547 461 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 105 352 1032
Size: 105 247 680
Fragments arranged by size:
680 247 105
TatI w'GTAC_w
Cuts at: 0 803 1032
Size: 803 229
Tth111I GACn'n_nGTC
Cuts at: 0 682 1032
Size: 682 350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 1032
Size: 184 848
Enzymes that do cut:
AarI AccI AlwNI ApaI BamHI BanII BbvCI BcgI
BclI BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I
BsaWI BseYI BsgI BsiHKAI Bsp1286I BspMI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI EaeI EcoICRI Eco57MI EcoO109I
Hin4I MmeI MscI MslI MspA1I NsiI NspI PasI
PpuMI PshAI PspOMI PsrI PstI PvuII SacI SanDI
ScaI SfcI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BciVI
BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PspXI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI XbaI XhoI XmaI XmnI ZraI