Restriction map for
pF1KE2525
(Linear) (Six-base) MAPSORT of: KIEE2525.seq Check: 28 from: 1 to: 1566
KIEE2525.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AccI GT'mk_AC
Cuts at: 0 1076 1566
Size: 1076 490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1230 1526 1566
Size: 889 341 296 40
Fragments arranged by size:
889 341 296 40
AleI CACnn'nnGTG
Cuts at: 0 304 1566
Size: 304 1262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 392 1487 1566
Size: 392 1095 79
Fragments arranged by size:
1095 392 79
ApaI G_GGCC'C
Cuts at: 0 163 1566
Size: 163 1403
ApoI r'AATT_y
Cuts at: 0 361 409 1081 1566
Size: 361 48 672 485
Fragments arranged by size:
672 485 361 48
AvaI C'yCGr_G
Cuts at: 0 14 91 219 1566
Size: 14 77 128 1347
Fragments arranged by size:
1347 128 77 14
BamHI G'GATC_C
Cuts at: 0 743 1427 1566
Size: 743 684 139
Fragments arranged by size:
743 684 139
BanI G'GyrC_C
Cuts at: 0 178 259 640 1566
Size: 178 81 381 926
Fragments arranged by size:
926 381 178 81
BanII G_rGCy'C
Cuts at: 0 21 98 163 1566
Size: 21 77 65 1403
Fragments arranged by size:
1403 77 65 21
BbeI G_GCGC'C
Cuts at: 0 182 1566
Size: 182 1384
BbsI GAAGACnn'nnnn_
Cuts at: 0 1236 1327 1566
Size: 1236 91 239
Fragments arranged by size:
1236 239 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 275 1566
Size: 241 34 1291
Fragments arranged by size:
1291 241 34
BclI T'GATC_A
Cuts at: 0 372 818 1566
Size: 372 446 748
Fragments arranged by size:
748 446 372
BfrBI ATG'CAT
Cuts at: 0 369 1566
Size: 369 1197
Bme1580I G_kGCm'C
Cuts at: 0 163 1566
Size: 163 1403
BmrI ACTGGGnnnn_n'
Cuts at: 0 1048 1566
Size: 1048 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1133 1566
Size: 865 268 433
Fragments arranged by size:
865 433 268
Bpu10I CC'TnA_GC
Cuts at: 0 22 648 1566
Size: 22 626 918
Fragments arranged by size:
918 626 22
BsaHI Gr'CG_yC
Cuts at: 0 179 1566
Size: 179 1387
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 853 1566
Size: 853 713
BseYI C'CCAG_C
Cuts at: 0 255 291 1566
Size: 255 36 1275
Fragments arranged by size:
1275 255 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 816 1566
Size: 734 82 750
Fragments arranged by size:
750 734 82
BsiEI CG_ry'CG
Cuts at: 0 251 696 1566
Size: 251 445 870
Fragments arranged by size:
870 445 251
BsmI GAATG_Cn'
Cuts at: 0 468 1566
Size: 468 1098
Bsp1286I G_dGCh'C
Cuts at: 0 21 98 163 1566
Size: 21 77 65 1403
Fragments arranged by size:
1403 77 65 21
BspHI T'CATG_A
Cuts at: 0 621 956 1566
Size: 621 335 610
Fragments arranged by size:
621 610 335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 706 1566
Size: 706 860
BsrDI GCAATG_nn'
Cuts at: 0 722 1566
Size: 722 844
BsrFI r'CCGG_y
Cuts at: 0 28 1566
Size: 28 1538
BssHII G'CGCG_C
Cuts at: 0 58 60 111 236 238 1566
Size: 58 2 51 125 2 1328
Fragments arranged by size:
1328 125 58 51 2 2
BssSI C'ACGA_G
Cuts at: 0 528 1566
Size: 528 1038
BstAPI GCAn_nnn'nTGC
Cuts at: 0 830 1162 1566
Size: 830 332 404
Fragments arranged by size:
830 404 332
BstYI r'GATC_y
Cuts at: 0 632 743 1427 1566
Size: 632 111 684 139
Fragments arranged by size:
684 632 139 111
BtgI C'CryG_G
Cuts at: 0 98 128 182 272 510 1566
Size: 98 30 54 90 238 1056
Fragments arranged by size:
1056 238 98 90 54 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 677 1566
Size: 677 889
BtsI GCAGTG_nn'
Cuts at: 0 1487 1566
Size: 1487 79
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 276 311 1566
Size: 276 35 1255
Fragments arranged by size:
1255 276 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1519 1566
Size: 1519 47
EaeI y'GGCC_r
Cuts at: 0 230 248 513 693 1167 1566
Size: 230 18 265 180 474 399
Fragments arranged by size:
474 399 265 230 180 18
EagI C'GGCC_G
Cuts at: 0 248 693 1566
Size: 248 445 873
Fragments arranged by size:
873 445 248
EarI CTCTTCn'nnn_
Cuts at: 0 1145 1541 1545 1566
Size: 1145 396 4 21
Fragments arranged by size:
1145 396 21 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 889 1133 1230 1526 1566
Size: 865 24 244 97 296 40
Fragments arranged by size:
865 296 244 97 40 24
EcoO109I rG'GnC_Cy
Cuts at: 0 159 1005 1020 1566
Size: 159 846 15 546
Fragments arranged by size:
846 546 159 15
EcoRI G'AATT_C
Cuts at: 0 361 1081 1566
Size: 361 720 485
Fragments arranged by size:
720 485 361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1159 1524 1566
Size: 1159 365 42
Fragments arranged by size:
1159 365 42
HaeII r_GCGC'y
Cuts at: 0 29 182 1566
Size: 29 153 1384
Fragments arranged by size:
1384 153 29
HincII GTy'rAC
Cuts at: 0 978 1566
Size: 978 588
KasI G'GCGC_C
Cuts at: 0 178 1566
Size: 178 1388
MfeI C'AATT_G
Cuts at: 0 414 1566
Size: 414 1152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 1566
Size: 294 1272
MscI TGG'CCA
Cuts at: 0 232 515 1566
Size: 232 283 1051
Fragments arranged by size:
1051 283 232
MslI CAynn'nnrTG
Cuts at: 0 304 374 727 961 1301 1566
Size: 304 70 353 234 340 265
Fragments arranged by size:
353 340 304 265 234 70
MspA1I CmG'CkG
Cuts at: 0 100 172 349 678 1566
Size: 100 72 177 329 888
Fragments arranged by size:
888 329 177 100 72
NaeI GCC'GGC
Cuts at: 0 30 1566
Size: 30 1536
NarI GG'CG_CC
Cuts at: 0 179 1566
Size: 179 1387
NcoI C'CATG_G
Cuts at: 0 128 1566
Size: 128 1438
NdeI CA'TA_TG
Cuts at: 0 1433 1566
Size: 1433 133
NgoMIV G'CCGG_C
Cuts at: 0 28 1566
Size: 28 1538
NsiI A_TGCA'T
Cuts at: 0 371 1566
Size: 371 1195
PasI CC'CwG_GG
Cuts at: 0 756 1566
Size: 756 810
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 1566
Size: 291 1275
PpuMI rG'GwC_Cy
Cuts at: 0 1005 1020 1566
Size: 1005 15 546
Fragments arranged by size:
1005 546 15
PshAI GACnn'nnGTC
Cuts at: 0 1061 1566
Size: 1061 505
PsiI TTA'TAA
Cuts at: 0 1223 1566
Size: 1223 343
PspOMI G'GGCC_C
Cuts at: 0 159 1566
Size: 159 1407
PstI C_TGCA'G
Cuts at: 0 139 936 1466 1566
Size: 139 797 530 100
Fragments arranged by size:
797 530 139 100
PvuII CAG'CTG
Cuts at: 0 349 678 1566
Size: 349 329 888
Fragments arranged by size:
888 349 329
SacII CC_GC'GG
Cuts at: 0 101 1566
Size: 101 1465
SanDI GG'GwC_CC
Cuts at: 0 1005 1566
Size: 1005 561
SapI GCTCTTCn'nnn_
Cuts at: 0 1145 1541 1566
Size: 1145 396 25
Fragments arranged by size:
1145 396 25
SbfI CC_TGCA'GG
Cuts at: 0 139 1566
Size: 139 1427
SexAI A'CCwGG_T
Cuts at: 0 200 1566
Size: 200 1366
SfcI C'TryA_G
Cuts at: 0 135 932 1462 1566
Size: 135 797 530 104
Fragments arranged by size:
797 530 135 104
SfoI GGC'GCC
Cuts at: 0 180 1566
Size: 180 1386
SgrAI Cr'CCGG_yG
Cuts at: 0 28 1566
Size: 28 1538
StyI C'CwwG_G
Cuts at: 0 128 1016 1566
Size: 128 888 550
Fragments arranged by size:
888 550 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 247 283 1436 1566
Size: 54 193 36 1153 130
Fragments arranged by size:
1153 193 130 54 36
TatI w'GTAC_w
Cuts at: 0 569 1566
Size: 569 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 1566
Size: 519 32 1015
Fragments arranged by size:
1015 519 32
Tth111I GACn'n_nGTC
Cuts at: 0 903 1566
Size: 903 663
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 292 1258 1566
Size: 270 22 966 308
Fragments arranged by size:
966 308 270 22
XmnI GAAnn'nnTTC
Cuts at: 0 1292 1566
Size: 1292 274
Enzymes that do cut:
AccI AcuI AleI AlfI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BcgI BclI BfrBI Bme1580I
BmrI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BssHII BssSI
BstAPI BstYI BtgI BtgZI BtsI CspCI DrdI EaeI
EagI EarI Eco57MI EcoO109I EcoRI FalI HaeII HincII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI PasI PflMI PpuMI PshAI
PsiI PspOMI PstI PvuII SacII SanDI SapI SbfI
SexAI SfcI SfoI SgrAI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BciVI BglI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrGI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI DraI DraIII EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KpnI
MluI NheI NotI NruI NspI PacI PciI PfoI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI
SalI ScaI SfiI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI XmaI
ZraI