Restriction map for
pF1KE2523
(Linear) (Six-base) MAPSORT of: KIEE2523.seq Check: 8255 from: 1 to: 3753
KIEE2523.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 928 3576 3753
Size: 928 2648 177
Fragments arranged by size:
2648 928 177
AatII G_ACGT'C
Cuts at: 0 596 3753
Size: 596 3157
AccI GT'mk_AC
Cuts at: 0 2333 3753
Size: 2333 1420
Acc65I G'GTAC_C
Cuts at: 0 1112 1197 3753
Size: 1112 85 2556
Fragments arranged by size:
2556 1112 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2270 2482 2488 3753
Size: 2270 212 6 1265
Fragments arranged by size:
2270 1265 212 6
AfeI AGC'GCT
Cuts at: 0 3691 3753
Size: 3691 62
AflIII A'CryG_T
Cuts at: 0 529 3366 3753
Size: 529 2837 387
Fragments arranged by size:
2837 529 387
AhdI GACnn_n'nnGTC
Cuts at: 0 108 1628 3207 3753
Size: 108 1520 1579 546
Fragments arranged by size:
1579 1520 546 108
AleI CACnn'nnGTG
Cuts at: 0 433 3753
Size: 433 3320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2607 3753
Size: 2607 1146
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 3353 3385 3753
Size: 538 32 2783 32 368
Fragments arranged by size:
2783 538 368 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 668 943 3753
Size: 668 275 2810
Fragments arranged by size:
2810 668 275
ApoI r'AATT_y
Cuts at: 0 247 578 712 875 1993 2423 3753
Size: 247 331 134 163 1118 430 1330
Fragments arranged by size:
1330 1118 430 331 247 163 134
AseI AT'TA_AT
Cuts at: 0 2282 3753
Size: 2282 1471
AvaI C'yCGr_G
Cuts at: 0 1333 2431 3753
Size: 1333 1098 1322
Fragments arranged by size:
1333 1322 1098
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1181 1214 3753
Size: 1181 33 2539
Fragments arranged by size:
2539 1181 33
BanI G'GyrC_C
Cuts at: 0 1112 1197 1666 3192 3721 3753
Size: 1112 85 469 1526 529 32
Fragments arranged by size:
1526 1112 529 469 85 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1136 2126 2258 3753
Size: 1136 990 132 1495
Fragments arranged by size:
1495 1136 990 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1648 1682 3753
Size: 1648 34 2071
Fragments arranged by size:
2071 1648 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3264 3753
Size: 3264 489
BclI T'GATC_A
Cuts at: 0 2699 3753
Size: 2699 1054
BglI GCCn_nnn'nGGC
Cuts at: 0 2032 3712 3753
Size: 2032 1680 41
Fragments arranged by size:
2032 1680 41
BglII A'GATC_T
Cuts at: 0 2180 3753
Size: 2180 1573
Bme1580I G_kGCm'C
Cuts at: 0 440 1671 1750 3753
Size: 440 1231 79 2003
Fragments arranged by size:
2003 1231 440 79
BmgBI CAC'GTC
Cuts at: 0 530 2691 3753
Size: 530 2161 1062
Fragments arranged by size:
2161 1062 530
BmrI ACTGGGnnnn_n'
Cuts at: 0 1269 1753 3753
Size: 1269 484 2000
Fragments arranged by size:
2000 1269 484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2216 3753
Size: 2216 1537
BsaAI yAC'GTr
Cuts at: 0 886 3753
Size: 886 2867
BsaHI Gr'CG_yC
Cuts at: 0 458 593 2330 3753
Size: 458 135 1737 1423
Fragments arranged by size:
1737 1423 458 135
BsaWI w'CCGG_w
Cuts at: 0 1796 3753
Size: 1796 1957
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3020 3050 3553 3583 3646 3676 3753
Size: 3020 30 503 30 63 30 77
Fragments arranged by size:
3020 503 77 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 2089 2983 3577 3654 3753
Size: 169 1920 894 594 77 99
Fragments arranged by size:
1920 894 594 169 99 77
BseYI C'CCAG_C
Cuts at: 0 453 3753
Size: 453 3300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2377 2598 3753
Size: 2377 221 1155
Fragments arranged by size:
2377 1155 221
BsiEI CG_ry'CG
Cuts at: 0 210 3753
Size: 210 3543
BsiHKAI G_wGCw'C
Cuts at: 0 2993 3753
Size: 2993 760
BsmI GAATG_Cn'
Cuts at: 0 2750 3300 3753
Size: 2750 550 453
Fragments arranged by size:
2750 550 453
BsmBI CGTCTCn'nnnn_
Cuts at: 0 584 3753
Size: 584 3169
Bsp1286I G_dGCh'C
Cuts at: 0 440 1671 1750 2993 3753
Size: 440 1231 79 1243 760
Fragments arranged by size:
1243 1231 760 440 79
BspHI T'CATG_A
Cuts at: 0 1388 3753
Size: 1388 2365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 542 928 1274 3576 3753
Size: 542 386 346 2302 177
Fragments arranged by size:
2302 542 386 346 177
BsrDI GCAATG_nn'
Cuts at: 0 3224 3317 3753
Size: 3224 93 436
Fragments arranged by size:
3224 436 93
BsrGI T'GTAC_A
Cuts at: 0 184 2972 3753
Size: 184 2788 781
Fragments arranged by size:
2788 781 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2344 3753
Size: 2344 1409
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2628 2664 3434 3753
Size: 2628 36 770 319
Fragments arranged by size:
2628 770 319 36
BstYI r'GATC_y
Cuts at: 0 1340 2180 3530 3753
Size: 1340 840 1350 223
Fragments arranged by size:
1350 1340 840 223
BtgI C'CryG_G
Cuts at: 0 918 1149 1193 2621 3725 3753
Size: 918 231 44 1428 1104 28
Fragments arranged by size:
1428 1104 918 231 44 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3526 3753
Size: 3526 227
BtsI GCAGTG_nn'
Cuts at: 0 461 3574 3753
Size: 461 3113 179
Fragments arranged by size:
3113 461 179
ClaI AT'CG_AT
Cuts at: 0 3130 3753
Size: 3130 623
DraI TTT'AAA
Cuts at: 0 710 984 1398 3753
Size: 710 274 414 2355
Fragments arranged by size:
2355 710 414 274
DraIII CAC_nnn'GTG
Cuts at: 0 2380 3753
Size: 2380 1373
EaeI y'GGCC_r
Cuts at: 0 1071 2666 2817 3511 3753
Size: 1071 1595 151 694 242
Fragments arranged by size:
1595 1071 694 242 151
EarI CTCTTCn'nnn_
Cuts at: 0 383 2294 2635 3145 3753
Size: 383 1911 341 510 608
Fragments arranged by size:
1911 608 510 383 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3574 3583 3730 3753
Size: 3574 9 147 23
Fragments arranged by size:
3574 147 23 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2216 2270 2482 2488 3753
Size: 2216 54 212 6 1265
Fragments arranged by size:
2216 1265 212 54 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1263 1715 3120 3753
Size: 1263 452 1405 633
Fragments arranged by size:
1405 1263 633 452
EcoRV GAT'ATC
Cuts at: 0 1932 2791 3753
Size: 1932 859 962
Fragments arranged by size:
1932 962 859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 112 875 3114 3753
Size: 112 763 2239 639
Fragments arranged by size:
2239 763 639 112
FspI TGC'GCA
Cuts at: 0 2927 3753
Size: 2927 826
HaeII r_GCGC'y
Cuts at: 0 3618 3693 3753
Size: 3618 75 60
Fragments arranged by size:
3618 75 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 1126 1158 1614 1646 2315 2347
Size: 94 32 1000 32 456 32 669 32
Cuts at: 2347 3193 3225 3646 3678 3753
Size: 846 32 421 32 75
Fragments arranged by size:
1000 846 669 456 421 94 75 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 141 3440 3608 3753
Size: 141 3299 168 145
Fragments arranged by size:
3299 168 145 141
HindIII A'AGCT_T
Cuts at: 0 1510 1843 3105 3753
Size: 1510 333 1262 648
Fragments arranged by size:
1510 1262 648 333
HpaI GTT'AAC
Cuts at: 0 3440 3753
Size: 3440 313
KpnI G_GTAC'C
Cuts at: 0 1116 1201 3753
Size: 1116 85 2552
Fragments arranged by size:
2552 1116 85
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 2703 3224 3753
Size: 567 2136 521 529
Fragments arranged by size:
2136 567 529 521
MscI TGG'CCA
Cuts at: 0 2668 3513 3753
Size: 2668 845 240
Fragments arranged by size:
2668 845 240
MslI CAynn'nnrTG
Cuts at: 0 433 1439 1609 2626 2836 3753
Size: 433 1006 170 1017 210 917
Fragments arranged by size:
1017 1006 917 433 210 170
MspA1I CmG'CkG
Cuts at: 0 628 2544 3642 3727 3753
Size: 628 1916 1098 85 26
Fragments arranged by size:
1916 1098 628 85 26
NcoI C'CATG_G
Cuts at: 0 1193 2621 3753
Size: 1193 1428 1132
Fragments arranged by size:
1428 1193 1132
NdeI CA'TA_TG
Cuts at: 0 1497 3753
Size: 1497 2256
NspI r_CATG'y
Cuts at: 0 3370 3753
Size: 3370 383
PasI CC'CwG_GG
Cuts at: 0 3046 3753
Size: 3046 707
PciI A'CATG_T
Cuts at: 0 3366 3753
Size: 3366 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 2380 3753
Size: 504 1876 1373
Fragments arranged by size:
1876 1373 504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2932 2964 3753
Size: 2932 32 789
Fragments arranged by size:
2932 789 32
PpuMI rG'GwC_Cy
Cuts at: 0 1263 1715 3120 3753
Size: 1263 452 1405 633
Fragments arranged by size:
1405 1263 633 452
PstI C_TGCA'G
Cuts at: 0 2798 3753
Size: 2798 955
PvuI CG_AT'CG
Cuts at: 0 210 3753
Size: 210 3543
PvuII CAG'CTG
Cuts at: 0 628 3753
Size: 628 3125
SacII CC_GC'GG
Cuts at: 0 3728 3753
Size: 3728 25
SapI GCTCTTCn'nnn_
Cuts at: 0 383 3753
Size: 383 3370
SexAI A'CCwGG_T
Cuts at: 0 476 2633 3753
Size: 476 2157 1120
Fragments arranged by size:
2157 1120 476
SfcI C'TryA_G
Cuts at: 0 69 2108 2128 2794 3753
Size: 69 2039 20 666 959
Fragments arranged by size:
2039 959 666 69 20
SmlI C'TyrA_G
Cuts at: 0 2431 3753
Size: 2431 1322
SpeI A'CTAG_T
Cuts at: 0 432 3753
Size: 432 3321
StyI C'CwwG_G
Cuts at: 0 1193 2621 3753
Size: 1193 1428 1132
Fragments arranged by size:
1428 1193 1132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2175 3753
Size: 2175 1578
TatI w'GTAC_w
Cuts at: 0 4 184 302 1274 2972 3731 3753
Size: 4 180 118 972 1698 759 22
Fragments arranged by size:
1698 972 759 180 118 22 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 840 872 2310 2342 3553 3585 3753
Size: 840 32 1438 32 1211 32 168
Fragments arranged by size:
1438 1211 840 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1244 3753
Size: 1244 2509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1740 2719 3753
Size: 1740 979 1034
Fragments arranged by size:
1740 1034 979
XhoI C'TCGA_G
Cuts at: 0 2431 3753
Size: 2431 1322
XmnI GAAnn'nnTTC
Cuts at: 0 62 1229 2426 3753
Size: 62 1167 1197 1327
Fragments arranged by size:
1327 1197 1167 62
ZraI GAC'GTC
Cuts at: 0 594 3753
Size: 594 3159
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApoI AseI AvaI BaeI
BanI BbsI BcgI BciVI BclI BglI BglII Bme1580I
BmgBI BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII EaeI EarI EciI Eco57MI
EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MmeI MscI MslI MspA1I NcoI NdeI
NspI PasI PciI PflMI PpiI PpuMI PstI PvuI
PvuII SacII SapI SexAI SfcI SmlI SpeI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI ApaI ApaLI AscI AsiSI AvrII
BamHI BanII BbeI BbvCI BfrBI BlpI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsiWI BspEI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I CspCI DrdI
EagI EcoICRI EcoNI EcoRI FseI FspAI KasI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI RsrII SacI SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
StuI SwaI XbaI XmaI