Restriction map for
pF1KE2522
(Linear) (Six-base) MAPSORT of: KIEE2522.seq Check: 6443 from: 1 to: 2772
KIEE2522.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 327 2772
Size: 327 2445
AccI GT'mk_AC
Cuts at: 0 1199 1640 2772
Size: 1199 441 1132
Fragments arranged by size:
1199 1132 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 894 2772
Size: 557 337 1878
Fragments arranged by size:
1878 557 337
AleI CACnn'nnGTG
Cuts at: 0 393 2772
Size: 393 2379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1333 2772
Size: 1333 1439
AlwNI CAG_nnn'CTG
Cuts at: 0 573 917 1374 1764 1857 2273 2457 2585
Size: 573 344 457 390 93 416 184 128
Cuts at: 2585 2772
Size: 187
Fragments arranged by size:
573 457 416 390 344 187 184 128
93
ApaI G_GGCC'C
Cuts at: 0 65 218 2772
Size: 65 153 2554
Fragments arranged by size:
2554 153 65
ApaLI G'TGCA_C
Cuts at: 0 2317 2772
Size: 2317 455
AvaI C'yCGr_G
Cuts at: 0 1693 2256 2772
Size: 1693 563 516
Fragments arranged by size:
1693 563 516
BamHI G'GATC_C
Cuts at: 0 343 821 2772
Size: 343 478 1951
Fragments arranged by size:
1951 478 343
BanI G'GyrC_C
Cuts at: 0 72 1285 2153 2186 2227 2772
Size: 72 1213 868 33 41 545
Fragments arranged by size:
1213 868 545 72 41 33
BanII G_rGCy'C
Cuts at: 0 65 218 545 976 1351 1811 1892 2350
Size: 65 153 327 431 375 460 81 458
Cuts at: 2350 2772
Size: 422
Fragments arranged by size:
460 458 431 422 375 327 153 81
65
BbeI G_GCGC'C
Cuts at: 0 1289 2157 2772
Size: 1289 868 615
Fragments arranged by size:
1289 868 615
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 1374 2772
Size: 639 735 1398
Fragments arranged by size:
1398 735 639
BbvCI CC'TCA_GC
Cuts at: 0 801 1174 2772
Size: 801 373 1598
Fragments arranged by size:
1598 801 373
BglI GCCn_nnn'nGGC
Cuts at: 0 14 1820 2236 2302 2772
Size: 14 1806 416 66 470
Fragments arranged by size:
1806 470 416 66 14
BglII A'GATC_T
Cuts at: 0 54 593 2340 2772
Size: 54 539 1747 432
Fragments arranged by size:
1747 539 432 54
BlpI GC'TnA_GC
Cuts at: 0 970 1345 2266 2772
Size: 970 375 921 506
Fragments arranged by size:
970 921 506 375
Bme1580I G_kGCm'C
Cuts at: 0 65 77 218 400 1947 2168 2189 2321
Size: 65 12 141 182 1547 221 21 132
Cuts at: 2321 2772
Size: 451
Fragments arranged by size:
1547 451 221 182 141 132 65 21
12
BmgBI CAC'GTC
Cuts at: 0 1380 1653 2772
Size: 1380 273 1119
Fragments arranged by size:
1380 1119 273
BmrI ACTGGGnnnn_n'
Cuts at: 0 801 927 2453 2772
Size: 801 126 1526 319
Fragments arranged by size:
1526 801 319 126
BmtI G_CTAG'C
Cuts at: 0 1660 2772
Size: 1660 1112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 990 2772
Size: 990 1782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 658 813 1903 1969 2574 2634 2772
Size: 481 177 155 1090 66 605 60 138
Fragments arranged by size:
1090 605 481 177 155 138 66 60
Bpu10I CC'TnA_GC
Cuts at: 0 801 1174 2772
Size: 801 373 1598
Fragments arranged by size:
1598 801 373
BsaI GGTCTCn'nnnn_
Cuts at: 0 1878 2772
Size: 1878 894
BsaAI yAC'GTr
Cuts at: 0 1395 1671 2772
Size: 1395 276 1101
Fragments arranged by size:
1395 1101 276
BsaBI GATnn'nnATC
Cuts at: 0 145 2772
Size: 145 2627
BsaHI Gr'CG_yC
Cuts at: 0 1286 2154 2772
Size: 1286 868 618
Fragments arranged by size:
1286 868 618
BsaWI w'CCGG_w
Cuts at: 0 1728 2772
Size: 1728 1044
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 366 396 1879 1909 2772
Size: 366 30 1483 30 863
Fragments arranged by size:
1483 863 366 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 92 95 98 180 523 838 979
Size: 70 22 3 3 82 343 315 141
Cuts at: 979 1819 1894 1900 2635 2772
Size: 840 75 6 735 137
Fragments arranged by size:
840 735 343 315 141 137 82 75
70 22 6 3 3
BseYI C'CCAG_C
Cuts at: 0 17 39 279 654 1244 1301 1425 1990
Size: 17 22 240 375 590 57 124 565
Cuts at: 1990 2419 2462 2772
Size: 429 43 310
Fragments arranged by size:
590 565 429 375 310 240 124 57
43 22 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1015 1060 1360 2259 2497 2772
Size: 934 81 45 300 899 238 275
Fragments arranged by size:
934 899 300 275 238 81 45
BsiHKAI G_wGCw'C
Cuts at: 0 270 976 1634 1670 1676 1892 2267 2321
Size: 270 706 658 36 6 216 375 54
Cuts at: 2321 2772
Size: 451
Fragments arranged by size:
706 658 451 375 270 216 54 36
6
Bsp1286I G_dGCh'C
Cuts at: 0 65 77 218 270 400 545 976 1351
Size: 65 12 141 52 130 145 431 375
Cuts at: 1351 1634 1670 1676 1811 1892 1947 2168 2189
Size: 283 36 6 135 81 55 221 21
Cuts at: 2189 2267 2321 2350 2772
Size: 78 54 29 422
Fragments arranged by size:
431 422 375 283 221 145 141 135
130 81 78 65 55 54 52 36
29 21 12 6
BspEI T'CCGG_A
Cuts at: 0 1728 2772
Size: 1728 1044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 327 1211 1359 2519 2772
Size: 315 12 884 148 1160 253
Fragments arranged by size:
1160 884 315 253 148 12
BsrBI CCG'CTC
Cuts at: 0 456 827 1025 1870 1902 2772
Size: 456 371 198 845 32 870
Fragments arranged by size:
870 845 456 371 198 32
BsrDI GCAATG_nn'
Cuts at: 0 317 2772
Size: 317 2455
BsrFI r'CCGG_y
Cuts at: 0 865 1131 1838 2772
Size: 865 266 707 934
Fragments arranged by size:
934 865 707 266
BssHII G'CGCG_C
Cuts at: 0 1297 1421 1432 1453 1497 1499 1501 2772
Size: 1297 124 11 21 44 2 2 1271
Fragments arranged by size:
1297 1271 124 44 21 11 2 2
BssSI C'ACGA_G
Cuts at: 0 1489 1940 2772
Size: 1489 451 832
Fragments arranged by size:
1489 832 451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 522 1374 1494 1764 1857 2772
Size: 522 852 120 270 93 915
Fragments arranged by size:
915 852 522 270 120 93
BstEII G'GTnAC_C
Cuts at: 0 1698 2724 2772
Size: 1698 1026 48
Fragments arranged by size:
1698 1026 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 616 2772
Size: 616 2156
BstYI r'GATC_y
Cuts at: 0 54 140 343 593 821 2340 2772
Size: 54 86 203 250 228 1519 432
Fragments arranged by size:
1519 432 250 228 203 86 54
Bsu36I CC'TnA_GG
Cuts at: 0 476 2291 2772
Size: 476 1815 481
Fragments arranged by size:
1815 481 476
BtgI C'CryG_G
Cuts at: 0 501 1045 1179 1215 1236 1661 2037 2548
Size: 501 544 134 36 21 425 376 511
Cuts at: 2548 2772
Size: 224
Fragments arranged by size:
544 511 501 425 376 224 134 36
21
BtsI GCAGTG_nn'
Cuts at: 0 91 400 1012 1167 1744 2772
Size: 91 309 612 155 577 1028
Fragments arranged by size:
1028 612 577 309 155 91
EaeI y'GGCC_r
Cuts at: 0 1112 1325 2303 2372 2772
Size: 1112 213 978 69 400
Fragments arranged by size:
1112 978 400 213 69
EarI CTCTTCn'nnn_
Cuts at: 0 913 1216 1237 1618 1727 2317 2772
Size: 913 303 21 381 109 590 455
Fragments arranged by size:
913 590 455 381 303 109 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1924 2772
Size: 1924 848
EcoICRI GAG'CTC
Cuts at: 0 974 1890 2772
Size: 974 916 882
Fragments arranged by size:
974 916 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 557 658 813 894 1903 1969 2574
Size: 481 76 101 155 81 1009 66 605
Cuts at: 2574 2634 2772
Size: 60 138
Fragments arranged by size:
1009 605 481 155 138 101 81 76
66 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 131 1801 1882 2290 2772
Size: 131 1670 81 408 482
Fragments arranged by size:
1670 482 408 131 81
EcoO109I rG'GnC_Cy
Cuts at: 0 61 124 214 215 427 482 945 1029
Size: 61 63 90 1 212 55 463 84
Cuts at: 1029 2415 2582 2772
Size: 1386 167 190
Fragments arranged by size:
1386 463 212 190 167 90 84 63
61 55 1
FspI TGC'GCA
Cuts at: 0 1543 1570 2772
Size: 1543 27 1202
Fragments arranged by size:
1543 1202 27
HaeII r_GCGC'y
Cuts at: 0 1111 1192 1289 1345 2037 2157 2772
Size: 1111 81 97 56 692 120 615
Fragments arranged by size:
1111 692 615 120 97 81 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 125 157 982 1014 1879 1911 2407 2439
Size: 125 32 825 32 865 32 496 32
Cuts at: 2439 2614 2646 2743 2772
Size: 175 32 97 29
Fragments arranged by size:
865 825 496 175 125 97 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 0 1200 2772
Size: 1200 1572
KasI G'GCGC_C
Cuts at: 0 1285 2153 2772
Size: 1285 868 619
Fragments arranged by size:
1285 868 619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 2772
Size: 906 1866
MscI TGG'CCA
Cuts at: 0 1114 2305 2772
Size: 1114 1191 467
Fragments arranged by size:
1191 1114 467
MslI CAynn'nnrTG
Cuts at: 0 393 928 1681 2772
Size: 393 535 753 1091
Fragments arranged by size:
1091 753 535 393
MspA1I CmG'CkG
Cuts at: 0 612 654 837 1020 1047 1139 1146 1181
Size: 612 42 183 183 27 92 7 35
Cuts at: 1181 1217 1238 1281 1353 1464 1549 1854 1914
Size: 36 21 43 72 111 85 305 60
Cuts at: 1914 2218 2368 2772
Size: 304 150 404
Fragments arranged by size:
612 404 305 304 183 183 150 111
92 85 72 60 43 42 36 35
27 21 7
NaeI GCC'GGC
Cuts at: 0 867 1133 2772
Size: 867 266 1639
Fragments arranged by size:
1639 867 266
NarI GG'CG_CC
Cuts at: 0 1286 2154 2772
Size: 1286 868 618
Fragments arranged by size:
1286 868 618
NgoMIV G'CCGG_C
Cuts at: 0 865 1131 2772
Size: 865 266 1641
Fragments arranged by size:
1641 865 266
NheI G'CTAG_C
Cuts at: 0 1656 2772
Size: 1656 1116
NspI r_CATG'y
Cuts at: 0 1791 2772
Size: 1791 981
PasI CC'CwG_GG
Cuts at: 0 983 1151 2161 2772
Size: 983 168 1010 611
Fragments arranged by size:
1010 983 611 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 2457 2772
Size: 573 1884 315
Fragments arranged by size:
1884 573 315
PfoI T'CCnGG_A
Cuts at: 0 1799 1880 2772
Size: 1799 81 892
Fragments arranged by size:
1799 892 81
PmlI CAC'GTG
Cuts at: 0 1395 1671 2772
Size: 1395 276 1101
Fragments arranged by size:
1395 1101 276
PpuMI rG'GwC_Cy
Cuts at: 0 124 482 2415 2772
Size: 124 358 1933 357
Fragments arranged by size:
1933 358 357 124
PspOMI G'GGCC_C
Cuts at: 0 61 214 2772
Size: 61 153 2558
Fragments arranged by size:
2558 153 61
PstI C_TGCA'G
Cuts at: 0 1019 1772 1862 2565 2772
Size: 1019 753 90 703 207
Fragments arranged by size:
1019 753 703 207 90
PvuII CAG'CTG
Cuts at: 0 654 1464 1854 2218 2368 2772
Size: 654 810 390 364 150 404
Fragments arranged by size:
810 654 404 390 364 150
SacI G_AGCT'C
Cuts at: 0 976 1892 2772
Size: 976 916 880
Fragments arranged by size:
976 916 880
SacII CC_GC'GG
Cuts at: 0 1048 1182 1218 1239 2772
Size: 1048 134 36 21 1533
Fragments arranged by size:
1533 1048 134 36 21
SalI G'TCGA_C
Cuts at: 0 1198 2772
Size: 1198 1574
SanDI GG'GwC_CC
Cuts at: 0 482 2772
Size: 482 2290
SapI GCTCTTCn'nnn_
Cuts at: 0 1216 1237 1727 2772
Size: 1216 21 490 1045
Fragments arranged by size:
1216 1045 490 21
SexAI A'CCwGG_T
Cuts at: 0 1100 2772
Size: 1100 1672
SfcI C'TryA_G
Cuts at: 0 232 597 1015 1386 1768 1858 2561 2772
Size: 232 365 418 371 382 90 703 211
Fragments arranged by size:
703 418 382 371 365 232 211 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2302 2772
Size: 2302 470
SfoI GGC'GCC
Cuts at: 0 1287 2155 2772
Size: 1287 868 617
Fragments arranged by size:
1287 868 617
SmaI CCC'GGG
Cuts at: 0 2258 2772
Size: 2258 514
StuI AGG'CCT
Cuts at: 0 1124 2077 2296 2772
Size: 1124 953 219 476
Fragments arranged by size:
1124 953 476 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 510 2752 2772
Size: 510 2242 20
Fragments arranged by size:
2242 510 20
TatI w'GTAC_w
Cuts at: 0 2202 2772
Size: 2202 570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1723 1755 2772
Size: 1723 32 1017
Fragments arranged by size:
1723 1017 32
Tth111I GACn'n_nGTC
Cuts at: 0 780 2772
Size: 780 1992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 409 613 751 837 1101 2772
Size: 360 49 204 138 86 264 1671
Fragments arranged by size:
1671 360 264 204 138 86 49
XmaI C'CCGG_G
Cuts at: 0 2256 2772
Size: 2256 516
XmnI GAAnn'nnTTC
Cuts at: 0 2704 2772
Size: 2704 68
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SalI SanDI SapI SexAI SfcI SfiI SfoI
SmaI StuI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BciVI BclI BfrBI BpuEI BsiEI BsiWI BsmI BsmBI
BspHI BsrGI BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SbfI ScaI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
XbaI XhoI ZraI