Restriction map for
pF1KE2515
(Linear) (Six-base) MAPSORT of: KIEE2515.seq Check: 150 from: 1 to: 1113
KIEE2515.orf
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AatII G_ACGT'C
Cuts at: 0 284 1113
Size: 284 829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1113
Size: 220 893
AflIII A'CryG_T
Cuts at: 0 653 668 1113
Size: 653 15 445
Fragments arranged by size:
653 445 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 339 371 1113
Size: 339 32 742
Fragments arranged by size:
742 339 32
ApaI G_GGCC'C
Cuts at: 0 333 1113
Size: 333 780
ApaLI G'TGCA_C
Cuts at: 0 841 1113
Size: 841 272
ApoI r'AATT_y
Cuts at: 0 36 1113
Size: 36 1077
AvaI C'yCGr_G
Cuts at: 0 288 539 913 1113
Size: 288 251 374 200
Fragments arranged by size:
374 288 251 200
BanI G'GyrC_C
Cuts at: 0 251 292 555 997 1113
Size: 251 41 263 442 116
Fragments arranged by size:
442 263 251 116 41
BanII G_rGCy'C
Cuts at: 0 333 452 704 1113
Size: 333 119 252 409
Fragments arranged by size:
409 333 252 119
BbeI G_GCGC'C
Cuts at: 0 559 1113
Size: 559 554
BbsI GAAGACnn'nnnn_
Cuts at: 0 794 982 1113
Size: 794 188 131
Fragments arranged by size:
794 188 131
BbvCI CC'TCA_GC
Cuts at: 0 131 1113
Size: 131 982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 358 1113
Size: 324 34 755
Fragments arranged by size:
755 324 34
BglI GCCn_nnn'nGGC
Cuts at: 0 52 853 1113
Size: 52 801 260
Fragments arranged by size:
801 260 52
BglII A'GATC_T
Cuts at: 0 989 1113
Size: 989 124
Bme1580I G_kGCm'C
Cuts at: 0 295 333 845 1113
Size: 295 38 512 268
Fragments arranged by size:
512 295 268 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 448 1113
Size: 448 665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1113
Size: 55 1058
Bpu10I CC'TnA_GC
Cuts at: 0 131 1113
Size: 131 982
BsaAI yAC'GTr
Cuts at: 0 411 840 1113
Size: 411 429 273
Fragments arranged by size:
429 411 273
BsaHI Gr'CG_yC
Cuts at: 0 281 556 1113
Size: 281 275 557
Fragments arranged by size:
557 281 275
BsaWI w'CCGG_w
Cuts at: 0 1103 1113
Size: 1103 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 217 247 640 670 1113
Size: 148 30 39 30 393 30 443
Fragments arranged by size:
443 393 148 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 454 1000 1113
Size: 205 249 546 113
Fragments arranged by size:
546 249 205 113
BsiHKAI G_wGCw'C
Cuts at: 0 452 533 845 1113
Size: 452 81 312 268
Fragments arranged by size:
452 312 268 81
Bsp1286I G_dGCh'C
Cuts at: 0 295 333 452 533 704 845 1113
Size: 295 38 119 81 171 141 268
Fragments arranged by size:
295 268 171 141 119 81 38
BsrFI r'CCGG_y
Cuts at: 0 248 295 614 895 1113
Size: 248 47 319 281 218
Fragments arranged by size:
319 281 248 218 47
BssSI C'ACGA_G
Cuts at: 0 187 1113
Size: 187 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 305 1113
Size: 305 808
BstEII G'GTnAC_C
Cuts at: 0 414 735 1113
Size: 414 321 378
Fragments arranged by size:
414 378 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 732 1056 1113
Size: 732 324 57
Fragments arranged by size:
732 324 57
BstYI r'GATC_y
Cuts at: 0 635 989 1113
Size: 635 354 124
Fragments arranged by size:
635 354 124
BtsI GCAGTG_nn'
Cuts at: 0 120 1113
Size: 120 993
DraIII CAC_nnn'GTG
Cuts at: 0 414 1113
Size: 414 699
EaeI y'GGCC_r
Cuts at: 0 99 602 689 1113
Size: 99 503 87 424
Fragments arranged by size:
503 424 99 87
EarI CTCTTCn'nnn_
Cuts at: 0 262 508 1113
Size: 262 246 605
Fragments arranged by size:
605 262 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 1113
Size: 121 992
EcoICRI GAG'CTC
Cuts at: 0 450 1113
Size: 450 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 220 1113
Size: 55 165 893
Fragments arranged by size:
893 165 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 754 1113
Size: 259 495 359
Fragments arranged by size:
495 359 259
EcoO109I rG'GnC_Cy
Cuts at: 0 329 1035 1113
Size: 329 706 78
Fragments arranged by size:
706 329 78
FspI TGC'GCA
Cuts at: 0 214 1113
Size: 214 899
FspAI rTGC'GCAy
Cuts at: 0 214 1113
Size: 214 899
HaeII r_GCGC'y
Cuts at: 0 559 1113
Size: 559 554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 271 303 1113
Size: 127 32 112 32 810
Fragments arranged by size:
810 127 112 32 32
KasI G'GCGC_C
Cuts at: 0 555 1113
Size: 555 558
MscI TGG'CCA
Cuts at: 0 101 691 1113
Size: 101 590 422
Fragments arranged by size:
590 422 101
MslI CAynn'nnrTG
Cuts at: 0 1006 1113
Size: 1006 107
MspA1I CmG'CkG
Cuts at: 0 732 1074 1113
Size: 732 342 39
Fragments arranged by size:
732 342 39
NarI GG'CG_CC
Cuts at: 0 556 1113
Size: 556 557
NspI r_CATG'y
Cuts at: 0 312 672 1011 1113
Size: 312 360 339 102
Fragments arranged by size:
360 339 312 102
PciI A'CATG_T
Cuts at: 0 668 1113
Size: 668 445
PmlI CAC'GTG
Cuts at: 0 411 1113
Size: 411 702
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1113
Size: 1035 78
PspOMI G'GGCC_C
Cuts at: 0 329 1113
Size: 329 784
PvuII CAG'CTG
Cuts at: 0 732 1074 1113
Size: 732 342 39
Fragments arranged by size:
732 342 39
SacI G_AGCT'C
Cuts at: 0 452 1113
Size: 452 661
SfoI GGC'GCC
Cuts at: 0 557 1113
Size: 557 556
SmaI CCC'GGG
Cuts at: 0 541 1113
Size: 541 572
SphI G_CATG'C
Cuts at: 0 1011 1113
Size: 1011 102
StyI C'CwwG_G
Cuts at: 0 50 320 1113
Size: 50 270 793
Fragments arranged by size:
793 270 50
TatI w'GTAC_w
Cuts at: 0 620 1113
Size: 620 493
XmaI C'CCGG_G
Cuts at: 0 539 1113
Size: 539 574
ZraI GAC'GTC
Cuts at: 0 282 1113
Size: 282 831
Enzymes that do cut:
AatII AcuI AflIII AloI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BglII
Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BsiHKAI Bsp1286I BsrFI BssSI BstAPI BstEII BstXI
BstYI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI MscI
MslI MspA1I NarI NspI PciI PmlI PpuMI PspOMI
PvuII SacI SfoI SmaI SphI StyI TatI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
BpuEI BsaI BsaBI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FalI FseI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmnI