Restriction map for
pF1KE2510
(Linear) (Six-base) MAPSORT of: KIEE2510.seq Check: 7400 from: 1 to: 3378
KIEE2510.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 246 1719 1974 2235 2298 2521 3066 3378
Size: 246 1473 255 261 63 223 545 312
Fragments arranged by size:
1473 545 312 261 255 246 223 63
AatII G_ACGT'C
Cuts at: 0 1523 3378
Size: 1523 1855
AccI GT'mk_AC
Cuts at: 0 589 3378
Size: 589 2789
AclI AA'CG_TT
Cuts at: 0 167 3378
Size: 167 3211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1847 3378
Size: 781 1066 1531
Fragments arranged by size:
1531 1066 781
AgeI A'CCGG_T
Cuts at: 0 332 3378
Size: 332 3046
AleI CACnn'nnGTG
Cuts at: 0 1513 2393 3378
Size: 1513 880 985
Fragments arranged by size:
1513 985 880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 892 1587 3378
Size: 892 695 1791
Fragments arranged by size:
1791 892 695
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 3378
Size: 1510 32 1836
Fragments arranged by size:
1836 1510 32
AlwNI CAG_nnn'CTG
Cuts at: 0 192 411 435 903 993 1246 1293 1769
Size: 192 219 24 468 90 253 47 476
Cuts at: 1769 3378
Size: 1609
Fragments arranged by size:
1609 476 468 253 219 192 90 47
24
ApaI G_GGCC'C
Cuts at: 0 77 218 1801 2113 2686 2740 3044 3199
Size: 77 141 1583 312 573 54 304 155
Cuts at: 3199 3378
Size: 179
Fragments arranged by size:
1583 573 312 304 179 155 141 77
54
ApaLI G'TGCA_C
Cuts at: 0 1273 3378
Size: 1273 2105
AvaI C'yCGr_G
Cuts at: 0 116 385 2391 2406 2650 3378
Size: 116 269 2006 15 244 728
Fragments arranged by size:
2006 728 269 244 116 15
BamHI G'GATC_C
Cuts at: 0 1106 3378
Size: 1106 2272
BanI G'GyrC_C
Cuts at: 0 391 414 600 1334 1404 1840 2176 2281
Size: 391 23 186 734 70 436 336 105
Cuts at: 2281 2395 2996 3150 3180 3378
Size: 114 601 154 30 198
Fragments arranged by size:
734 601 436 391 336 198 186 154
114 105 70 30 23
BanII G_rGCy'C
Cuts at: 0 46 77 218 1685 1801 2106 2113 2241
Size: 46 31 141 1467 116 305 7 128
Cuts at: 2241 2345 2686 2731 2740 3017 3044 3199 3260
Size: 104 341 45 9 277 27 155 61
Cuts at: 3260 3378
Size: 118
Fragments arranged by size:
1467 341 305 277 155 141 128 118
116 104 61 46 45 31 27 9
7
BbeI G_GCGC'C
Cuts at: 0 604 3000 3378
Size: 604 2396 378
Fragments arranged by size:
2396 604 378
BbsI GAAGACnn'nnnn_
Cuts at: 0 1055 1780 3378
Size: 1055 725 1598
Fragments arranged by size:
1598 1055 725
BbvCI CC'TCA_GC
Cuts at: 0 825 961 1330 1971 2455 3378
Size: 825 136 369 641 484 923
Fragments arranged by size:
923 825 641 484 369 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1179 1213 2974 3008 3356 3378
Size: 1179 34 1761 34 348 22
Fragments arranged by size:
1761 1179 348 34 34 22
BclI T'GATC_A
Cuts at: 0 578 3378
Size: 578 2800
BfrBI ATG'CAT
Cuts at: 0 1377 3378
Size: 1377 2001
BglI GCCn_nnn'nGGC
Cuts at: 0 29 1348 1936 3378
Size: 29 1319 588 1442
Fragments arranged by size:
1442 1319 588 29
BglII A'GATC_T
Cuts at: 0 1894 3378
Size: 1894 1484
BlpI GC'TnA_GC
Cuts at: 0 2231 2240 2923 3378
Size: 2231 9 683 455
Fragments arranged by size:
2231 683 455 9
Bme1580I G_kGCm'C
Cuts at: 0 77 218 986 1277 1407 1801 1843 1851
Size: 77 141 768 291 130 394 42 8
Cuts at: 1851 2113 2181 2284 2520 2686 2740 3044 3153
Size: 262 68 103 236 166 54 304 109
Cuts at: 3153 3199 3378
Size: 46 179
Fragments arranged by size:
768 394 304 291 262 236 179 166
141 130 109 103 77 68 54 46
42 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 71 710 1796 1798 2562 2696 2710 3115
Size: 71 639 1086 2 764 134 14 405
Cuts at: 3115 3378
Size: 263
Fragments arranged by size:
1086 764 639 405 263 134 71 14
2
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2747 3378
Size: 2747 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 248 457 1339 1453 1654 2347 2742
Size: 185 63 209 882 114 201 693 395
Cuts at: 2742 2847 3378
Size: 105 531
Fragments arranged by size:
882 693 531 395 209 201 185 114
105 63
Bpu10I CC'TnA_GC
Cuts at: 0 825 961 1330 1971 2455 2524 3011 3203
Size: 825 136 369 641 484 69 487 192
Cuts at: 3203 3378
Size: 175
Fragments arranged by size:
825 641 487 484 369 192 175 136
69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2466 3378
Size: 2466 912
BsaHI Gr'CG_yC
Cuts at: 0 541 601 1520 1672 2066 2997 3378
Size: 541 60 919 152 394 931 381
Fragments arranged by size:
931 919 541 394 381 152 60
BsaWI w'CCGG_w
Cuts at: 0 290 332 347 366 3378
Size: 290 42 15 19 3012
Fragments arranged by size:
3012 290 42 19 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 620 650 1420 1450 1903 1933
Size: 430 30 160 30 770 30 453 30
Cuts at: 1933 2882 2912 3378
Size: 949 30 466
Fragments arranged by size:
949 770 466 453 430 160 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 104 517 550 1267 1270 1924 2057 2472
Size: 104 413 33 717 3 654 133 415
Cuts at: 2472 2739 3060 3063 3378
Size: 267 321 3 315
Fragments arranged by size:
717 654 415 413 321 315 267 133
104 33 3 3
BseYI C'CCAG_C
Cuts at: 0 67 217 1665 1833 1947 1954 2202 2519
Size: 67 150 1448 168 114 7 248 317
Cuts at: 2519 2619 2709 2981 3334 3378
Size: 100 90 272 353 44
Fragments arranged by size:
1448 353 317 272 248 168 150 114
100 90 67 44 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 274 1501 2106 2729 3378
Size: 70 204 1227 605 623 649
Fragments arranged by size:
1227 649 623 605 204 70
BsiEI CG_ry'CG
Cuts at: 0 516 569 2004 3378
Size: 516 53 1435 1374
Fragments arranged by size:
1435 1374 516 53
BsiHKAI G_wGCw'C
Cuts at: 0 488 959 1277 1965 2731 3260 3378
Size: 488 471 318 688 766 529 118
Fragments arranged by size:
766 688 529 488 471 318 118
BsmI GAATG_Cn'
Cuts at: 0 1129 3378
Size: 1129 2249
BsmBI CGTCTCn'nnnn_
Cuts at: 0 821 3378
Size: 821 2557
Bsp1286I G_dGCh'C
Cuts at: 0 46 77 218 488 959 986 1277 1407
Size: 46 31 141 270 471 27 291 130
Cuts at: 1407 1685 1801 1843 1851 1965 2106 2113 2181
Size: 278 116 42 8 114 141 7 68
Cuts at: 2181 2241 2284 2345 2520 2686 2731 2740 3017
Size: 60 43 61 175 166 45 9 277
Cuts at: 3017 3044 3153 3199 3260 3378
Size: 27 109 46 61 118
Fragments arranged by size:
471 291 278 277 270 175 166 141
141 130 118 116 114 109 68 61
61 60 46 46 45 43 42 31
27 27 9 8 7
BspEI T'CCGG_A
Cuts at: 0 290 366 3378
Size: 290 76 3012
Fragments arranged by size:
3012 290 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 246 1334 1421 1719 1974 2235 2298 2521
Size: 246 1088 87 298 255 261 63 223
Cuts at: 2521 3066 3378
Size: 545 312
Fragments arranged by size:
1088 545 312 298 261 255 246 223
87 63
BsrBI CCG'CTC
Cuts at: 0 744 2235 2315 2822 3378
Size: 744 1491 80 507 556
Fragments arranged by size:
1491 744 556 507 80
BsrFI r'CCGG_y
Cuts at: 0 282 332 515 856 1046 1549 1661 3378
Size: 282 50 183 341 190 503 112 1717
Fragments arranged by size:
1717 503 341 282 190 183 112 50
BsrGI T'GTAC_A
Cuts at: 0 3275 3378
Size: 3275 103
BssSI C'ACGA_G
Cuts at: 0 842 1087 3378
Size: 842 245 2291
Fragments arranged by size:
2291 842 245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 903 3378
Size: 903 2475
BstEII G'GTnAC_C
Cuts at: 0 258 3378
Size: 258 3120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1216 1431 3378
Size: 1216 215 1947
Fragments arranged by size:
1947 1216 215
BstYI r'GATC_y
Cuts at: 0 1040 1106 1894 3108 3378
Size: 1040 66 788 1214 270
Fragments arranged by size:
1214 1040 788 270 66
BstZ17I GTA'TAC
Cuts at: 0 590 3378
Size: 590 2788
Bsu36I CC'TnA_GG
Cuts at: 0 2355 3378
Size: 2355 1023
BtgI C'CryG_G
Cuts at: 0 130 835 1023 1424 1676 2471 3378
Size: 130 705 188 401 252 795 907
Fragments arranged by size:
907 795 705 401 252 188 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1907 3378
Size: 1907 1471
BtsI GCAGTG_nn'
Cuts at: 0 62 418 566 2137 3378
Size: 62 356 148 1571 1241
Fragments arranged by size:
1571 1241 356 148 62
DraIII CAC_nnn'GTG
Cuts at: 0 1029 1152 3378
Size: 1029 123 2226
Fragments arranged by size:
2226 1029 123
EaeI y'GGCC_r
Cuts at: 0 177 280 513 566 745 854 1391 2001
Size: 177 103 233 53 179 109 537 610
Cuts at: 2001 2249 2883 3378
Size: 248 634 495
Fragments arranged by size:
634 610 537 495 248 233 179 177
109 103 53
EagI C'GGCC_G
Cuts at: 0 513 566 2001 3378
Size: 513 53 1435 1377
Fragments arranged by size:
1435 1377 513 53
EarI CTCTTCn'nnn_
Cuts at: 0 94 739 3069 3081 3378
Size: 94 645 2330 12 297
Fragments arranged by size:
2330 645 297 94 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 781 1536 3378
Size: 781 755 1842
Fragments arranged by size:
1842 781 755
EcoICRI GAG'CTC
Cuts at: 0 2729 3258 3378
Size: 2729 529 120
Fragments arranged by size:
2729 529 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 248 457 781 1339 1453 1654 1847
Size: 185 63 209 324 558 114 201 193
Cuts at: 1847 2347 2742 2847 3378
Size: 500 395 105 531
Fragments arranged by size:
558 531 500 395 324 209 201 193
185 114 105 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 2484 3243 3378
Size: 532 1952 759 135
Fragments arranged by size:
1952 759 532 135
EcoO109I rG'GnC_Cy
Cuts at: 0 73 121 214 1313 1698 1797 1798 2052
Size: 73 48 93 1099 385 99 1 254
Cuts at: 2052 2109 2351 2403 2410 2477 2500 2655 2683
Size: 57 242 52 7 67 23 155 28
Cuts at: 2683 2737 2798 2906 3040 3049 3160 3187 3195
Size: 54 61 108 134 9 111 27 8
Cuts at: 3195 3196 3269 3378
Size: 1 73 109
Fragments arranged by size:
1099 385 254 242 155 134 111 109
108 99 93 73 73 67 61 57
54 52 48 28 27 23 9 8
7 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 1191 1797 2685 3378
Size: 711 480 606 888 693
Fragments arranged by size:
888 711 693 606 480
FseI GG_CCGG'CC
Cuts at: 0 860 3378
Size: 860 2518
HaeII r_GCGC'y
Cuts at: 0 604 3000 3378
Size: 604 2396 378
Fragments arranged by size:
2396 604 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 512 526 544 558 598 620
Size: 430 32 50 14 18 14 40 22
Cuts at: 620 622 630 652 654 1222 1254 1420 1452
Size: 2 8 22 2 568 32 166 32
Cuts at: 1452 1903 1935 2116 2148 3378
Size: 451 32 181 32 1230
Fragments arranged by size:
1230 568 451 430 181 166 50 40
32 32 32 32 32 22 22 18
14 14 8 2 2
HincII GTy'rAC
Cuts at: 0 171 3027 3378
Size: 171 2856 351
Fragments arranged by size:
2856 351 171
KasI G'GCGC_C
Cuts at: 0 600 2996 3378
Size: 600 2396 382
Fragments arranged by size:
2396 600 382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 3378
Size: 1091 2287
MscI TGG'CCA
Cuts at: 0 179 1393 2885 3378
Size: 179 1214 1492 493
Fragments arranged by size:
1492 1214 493 179
MslI CAynn'nnrTG
Cuts at: 0 493 874 1240 1372 1513 2393 3378
Size: 493 381 366 132 141 880 985
Fragments arranged by size:
985 880 493 381 366 141 132
MspA1I CmG'CkG
Cuts at: 0 132 829 900 912 1293 1571 1607 1749
Size: 132 697 71 12 381 278 36 142
Cuts at: 1749 1769 1934 2058 2466 3338 3378
Size: 20 165 124 408 872 40
Fragments arranged by size:
872 697 408 381 278 165 142 132
124 71 40 36 20 12
NaeI GCC'GGC
Cuts at: 0 517 858 1551 1663 3378
Size: 517 341 693 112 1715
Fragments arranged by size:
1715 693 517 341 112
NarI GG'CG_CC
Cuts at: 0 601 2997 3378
Size: 601 2396 381
Fragments arranged by size:
2396 601 381
NcoI C'CATG_G
Cuts at: 0 1424 2471 3378
Size: 1424 1047 907
Fragments arranged by size:
1424 1047 907
NgoMIV G'CCGG_C
Cuts at: 0 515 856 1549 1661 3378
Size: 515 341 693 112 1717
Fragments arranged by size:
1717 693 515 341 112
NotI GC'GGCC_GC
Cuts at: 0 566 3378
Size: 566 2812
NsiI A_TGCA'T
Cuts at: 0 1379 3378
Size: 1379 1999
NspI r_CATG'y
Cuts at: 0 654 1377 1381 2880 3378
Size: 654 723 4 1499 498
Fragments arranged by size:
1499 723 654 498 4
PasI CC'CwG_GG
Cuts at: 0 34 804 1656 2106 2170 2346 2627 2679
Size: 34 770 852 450 64 176 281 52
Cuts at: 2679 3044 3155 3191 3378
Size: 365 111 36 187
Fragments arranged by size:
852 770 450 365 281 187 176 111
64 52 36 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 411 1029 1400 3378
Size: 411 618 371 1978
Fragments arranged by size:
1978 618 411 371
PfoI T'CCnGG_A
Cuts at: 0 1109 1466 2824 3378
Size: 1109 357 1358 554
Fragments arranged by size:
1358 1109 554 357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 3378
Size: 229 32 3117
Fragments arranged by size:
3117 229 32
PpuMI rG'GwC_Cy
Cuts at: 0 1313 2351 2403 2410 2477 2655 2798 2906
Size: 1313 1038 52 7 67 178 143 108
Cuts at: 2906 3049 3160 3187 3269 3378
Size: 143 111 27 82 109
Fragments arranged by size:
1313 1038 178 143 143 111 109 108
82 67 52 27 7
PspOMI G'GGCC_C
Cuts at: 0 73 214 1797 2109 2682 2736 3040 3195
Size: 73 141 1583 312 573 54 304 155
Cuts at: 3195 3378
Size: 183
Fragments arranged by size:
1583 573 312 304 183 155 141 73
54
PspXI vC'TCGA_Gb
Cuts at: 0 116 3378
Size: 116 3262
PstI C_TGCA'G
Cuts at: 0 431 561 1139 1566 3378
Size: 431 130 578 427 1812
Fragments arranged by size:
1812 578 431 427 130
PvuII CAG'CTG
Cuts at: 0 829 1293 1607 1769 3338 3378
Size: 829 464 314 162 1569 40
Fragments arranged by size:
1569 829 464 314 162 40
SacI G_AGCT'C
Cuts at: 0 2731 3260 3378
Size: 2731 529 118
Fragments arranged by size:
2731 529 118
SacII CC_GC'GG
Cuts at: 0 133 3378
Size: 133 3245
SanDI GG'GwC_CC
Cuts at: 0 2403 2410 2655 2798 3049 3160 3187 3378
Size: 2403 7 245 143 251 111 27 191
Fragments arranged by size:
2403 251 245 191 143 111 27 7
SbfI CC_TGCA'GG
Cuts at: 0 1139 1566 3378
Size: 1139 427 1812
Fragments arranged by size:
1812 1139 427
SfcI C'TryA_G
Cuts at: 0 427 557 1135 1562 3219 3378
Size: 427 130 578 427 1657 159
Fragments arranged by size:
1657 578 427 427 159 130
SfoI GGC'GCC
Cuts at: 0 602 2998 3378
Size: 602 2396 380
Fragments arranged by size:
2396 602 380
SgrAI Cr'CCGG_yG
Cuts at: 0 332 3378
Size: 332 3046
SmaI CCC'GGG
Cuts at: 0 2393 2408 3378
Size: 2393 15 970
Fragments arranged by size:
2393 970 15
SmlI C'TyrA_G
Cuts at: 0 116 2481 3378
Size: 116 2365 897
Fragments arranged by size:
2365 897 116
SphI G_CATG'C
Cuts at: 0 1377 1381 2880 3378
Size: 1377 4 1499 498
Fragments arranged by size:
1499 1377 498 4
StyI C'CwwG_G
Cuts at: 0 1424 1694 2428 2471 2964 3378
Size: 1424 270 734 43 493 414
Fragments arranged by size:
1424 734 493 414 270 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 672 1422 1438 1525 1669 2107 3378
Size: 672 750 16 87 144 438 1271
Fragments arranged by size:
1271 750 672 438 144 87 16
TatI w'GTAC_w
Cuts at: 0 2771 3275 3378
Size: 2771 504 103
Fragments arranged by size:
2771 504 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 536 568 1138 1170 1212 1244 2882 2914
Size: 536 32 570 32 42 32 1638 32
Cuts at: 2914 3253 3285 3378
Size: 339 32 93
Fragments arranged by size:
1638 570 536 339 93 42 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 444 634 1882 3378
Size: 444 190 1248 1496
Fragments arranged by size:
1496 1248 444 190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 723 2272 2717 3378
Size: 723 1549 445 661
Fragments arranged by size:
1549 723 661 445
XhoI C'TCGA_G
Cuts at: 0 116 3378
Size: 116 3262
XmaI C'CCGG_G
Cuts at: 0 2391 2406 3378
Size: 2391 15 972
Fragments arranged by size:
2391 972 15
XmnI GAAnn'nnTTC
Cuts at: 0 1777 3102 3378
Size: 1777 1325 276
Fragments arranged by size:
1777 1325 276
ZraI GAC'GTC
Cuts at: 0 1521 3378
Size: 1521 1857
Enzymes that do cut:
AarI AatII AccI AclI AcuI AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NsiI NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SanDI SbfI
SfcI SfoI SgrAI SmaI SmlI SphI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AhdI ApoI AscI AseI
AsiSI AvrII BaeI BciVI BmgBI BmtI BsaI BsaAI
BsaBI BsiWI BspHI BsrDI BssHII BstBI ClaI CspCI
DraI DrdI EcoRI EcoRV FspI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NruI PacI PciI
PmeI PmlI PshAI PsiI PsrI PvuI RsrII SalI
SapI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI
SspI StuI SwaI XbaI