Restriction map for
pF1KE2496
(Linear) (Six-base) MAPSORT of: KIEE2496.seq Check: 995 from: 1 to: 1965
KIEE2496.orf
With 182 enzymes: SgfI *
January 16, 2014 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1317 1391 1925 1965
Size: 1317 74 534 40
Fragments arranged by size:
1317 534 74 40
AatII G_ACGT'C
Cuts at: 0 1166 1517 1965
Size: 1166 351 448
Fragments arranged by size:
1166 448 351
AccI GT'mk_AC
Cuts at: 0 701 1172 1965
Size: 701 471 793
Fragments arranged by size:
793 701 471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 788 1420 1816 1965
Size: 534 254 632 396 149
Fragments arranged by size:
632 534 396 254 149
AleI CACnn'nnGTG
Cuts at: 0 659 1965
Size: 659 1306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1399 1965
Size: 1399 566
AlwNI CAG_nnn'CTG
Cuts at: 0 625 688 1965
Size: 625 63 1277
Fragments arranged by size:
1277 625 63
ApaI G_GGCC'C
Cuts at: 0 66 823 1965
Size: 66 757 1142
Fragments arranged by size:
1142 757 66
ApaLI G'TGCA_C
Cuts at: 0 1686 1965
Size: 1686 279
ApoI r'AATT_y
Cuts at: 0 503 1876 1965
Size: 503 1373 89
Fragments arranged by size:
1373 503 89
AvaI C'yCGr_G
Cuts at: 0 590 601 893 1492 1767 1965
Size: 590 11 292 599 275 198
Fragments arranged by size:
599 590 292 275 198 11
BanI G'GyrC_C
Cuts at: 0 653 952 1016 1965
Size: 653 299 64 949
Fragments arranged by size:
949 653 299 64
BanII G_rGCy'C
Cuts at: 0 66 823 1311 1768 1965
Size: 66 757 488 457 197
Fragments arranged by size:
757 488 457 197 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 1937 1965
Size: 308 1629 28
Fragments arranged by size:
1629 308 28
BglI GCCn_nnn'nGGC
Cuts at: 0 322 827 1965
Size: 322 505 1138
Fragments arranged by size:
1138 505 322
Bme1580I G_kGCm'C
Cuts at: 0 66 352 823 1690 1852 1965
Size: 66 286 471 867 162 113
Fragments arranged by size:
867 471 286 162 113 66
BmgBI CAC'GTC
Cuts at: 0 1251 1965
Size: 1251 714
BmtI G_CTAG'C
Cuts at: 0 548 1965
Size: 548 1417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1735 1965
Size: 1585 150 230
Fragments arranged by size:
1585 230 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1965
Size: 1303 662
BsaI GGTCTCn'nnnn_
Cuts at: 0 1050 1965
Size: 1050 915
BsaHI Gr'CG_yC
Cuts at: 0 835 1163 1514 1965
Size: 835 328 351 451
Fragments arranged by size:
835 451 351 328
BsaWI w'CCGG_w
Cuts at: 0 1412 1965
Size: 1412 553
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 1618 1648 1831 1861 1965
Size: 499 30 1089 30 183 30 104
Fragments arranged by size:
1089 499 183 104 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 763 1210 1461 1510 1539 1639 1738 1852
Size: 763 447 251 49 29 100 99 114
Cuts at: 1852 1965
Size: 113
Fragments arranged by size:
763 447 251 114 113 100 99 49
29
BseYI C'CCAG_C
Cuts at: 0 681 919 1892 1965
Size: 681 238 973 73
Fragments arranged by size:
973 681 238 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 159 986 1117 1345 1359 1965
Size: 31 128 827 131 228 14 606
Fragments arranged by size:
827 606 228 131 128 31 14
BsiEI CG_ry'CG
Cuts at: 0 759 1809 1965
Size: 759 1050 156
Fragments arranged by size:
1050 759 156
BsiHKAI G_wGCw'C
Cuts at: 0 1311 1690 1768 1965
Size: 1311 379 78 197
Fragments arranged by size:
1311 379 197 78
Bsp1286I G_dGCh'C
Cuts at: 0 66 352 823 1311 1690 1768 1852 1965
Size: 66 286 471 488 379 78 84 113
Fragments arranged by size:
488 471 379 286 113 84 78 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1317 1391 1439 1709 1925 1965
Size: 1317 74 48 270 216 40
Fragments arranged by size:
1317 270 216 74 48 40
BsrDI GCAATG_nn'
Cuts at: 0 483 1965
Size: 483 1482
BsrFI r'CCGG_y
Cuts at: 0 95 275 316 758 1808 1965
Size: 95 180 41 442 1050 157
Fragments arranged by size:
1050 442 180 157 95 41
BsrGI T'GTAC_A
Cuts at: 0 8 971 1965
Size: 8 963 994
Fragments arranged by size:
994 963 8
BstXI CCAn_nnnn'nTGG
Cuts at: 0 83 201 1965
Size: 83 118 1764
Fragments arranged by size:
1764 118 83
Bsu36I CC'TnA_GG
Cuts at: 0 1343 1965
Size: 1343 622
BtgI C'CryG_G
Cuts at: 0 1633 1965
Size: 1633 332
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 593 1353 1965
Size: 593 760 612
Fragments arranged by size:
760 612 593
BtsI GCAGTG_nn'
Cuts at: 0 176 259 380 1965
Size: 176 83 121 1585
Fragments arranged by size:
1585 176 121 83
DraIII CAC_nnn'GTG
Cuts at: 0 54 1965
Size: 54 1911
DrdI GACnn_nn'nnGTC
Cuts at: 0 1042 1168 1965
Size: 1042 126 797
Fragments arranged by size:
1042 797 126
EaeI y'GGCC_r
Cuts at: 0 314 756 760 1806 1965
Size: 314 442 4 1046 159
Fragments arranged by size:
1046 442 314 159 4
EagI C'GGCC_G
Cuts at: 0 756 1806 1965
Size: 756 1050 159
Fragments arranged by size:
1050 756 159
EarI CTCTTCn'nnn_
Cuts at: 0 190 253 1965
Size: 190 63 1712
Fragments arranged by size:
1712 190 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 1038 1797 1965
Size: 30 1008 759 168
Fragments arranged by size:
1008 759 168 30
EcoICRI GAG'CTC
Cuts at: 0 1309 1766 1965
Size: 1309 457 199
Fragments arranged by size:
1309 457 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 788 1420 1585 1735 1816 1965
Size: 534 254 632 165 150 81 149
Fragments arranged by size:
632 534 254 165 150 149 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 973 1087 1435 1705 1965
Size: 907 66 114 348 270 260
Fragments arranged by size:
907 348 270 260 114 66
EcoO109I rG'GnC_Cy
Cuts at: 0 63 309 355 693 819 1599 1615 1710
Size: 63 246 46 338 126 780 16 95
Cuts at: 1710 1965
Size: 255
Fragments arranged by size:
780 338 255 246 126 95 63 46
16
EcoRI G'AATT_C
Cuts at: 0 1876 1965
Size: 1876 89
FseI GG_CCGG'CC
Cuts at: 0 762 1965
Size: 762 1203
FspI TGC'GCA
Cuts at: 0 736 1965
Size: 736 1229
FspAI rTGC'GCAy
Cuts at: 0 736 1965
Size: 736 1229
HaeII r_GCGC'y
Cuts at: 0 138 542 1409 1965
Size: 138 404 867 556
Fragments arranged by size:
867 556 404 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1531 1563 1965
Size: 1531 32 402
Fragments arranged by size:
1531 402 32
HincII GTy'rAC
Cuts at: 0 423 702 1965
Size: 423 279 1263
Fragments arranged by size:
1263 423 279
HindIII A'AGCT_T
Cuts at: 0 366 1965
Size: 366 1599
MslI CAynn'nnrTG
Cuts at: 0 659 1249 1385 1965
Size: 659 590 136 580
Fragments arranged by size:
659 590 580 136
MspA1I CmG'CkG
Cuts at: 0 32 141 168 941 1131 1214 1425 1896
Size: 32 109 27 773 190 83 211 471
Cuts at: 1896 1965
Size: 69
Fragments arranged by size:
773 471 211 190 109 83 69 32
27
NaeI GCC'GGC
Cuts at: 0 277 760 1810 1965
Size: 277 483 1050 155
Fragments arranged by size:
1050 483 277 155
NgoMIV G'CCGG_C
Cuts at: 0 275 758 1808 1965
Size: 275 483 1050 157
Fragments arranged by size:
1050 483 275 157
NheI G'CTAG_C
Cuts at: 0 544 1965
Size: 544 1421
NspI r_CATG'y
Cuts at: 0 1390 1827 1834 1965
Size: 1390 437 7 131
Fragments arranged by size:
1390 437 131 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 688 1965
Size: 688 1277
PfoI T'CCnGG_A
Cuts at: 0 1712 1965
Size: 1712 253
PpuMI rG'GwC_Cy
Cuts at: 0 693 1599 1710 1965
Size: 693 906 111 255
Fragments arranged by size:
906 693 255 111
PspOMI G'GGCC_C
Cuts at: 0 62 819 1965
Size: 62 757 1146
Fragments arranged by size:
1146 757 62
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1654 1686 1965
Size: 1654 32 279
Fragments arranged by size:
1654 279 32
PstI C_TGCA'G
Cuts at: 0 167 266 1088 1195 1706 1965
Size: 167 99 822 107 511 259
Fragments arranged by size:
822 511 259 167 107 99
PvuII CAG'CTG
Cuts at: 0 32 168 1131 1425 1896 1965
Size: 32 136 963 294 471 69
Fragments arranged by size:
963 471 294 136 69 32
SacI G_AGCT'C
Cuts at: 0 1311 1768 1965
Size: 1311 457 197
Fragments arranged by size:
1311 457 197
SalI G'TCGA_C
Cuts at: 0 700 1965
Size: 700 1265
SanDI GG'GwC_CC
Cuts at: 0 693 1599 1965
Size: 693 906 366
Fragments arranged by size:
906 693 366
SbfI CC_TGCA'GG
Cuts at: 0 1088 1965
Size: 1088 877
SfcI C'TryA_G
Cuts at: 0 163 262 1084 1191 1593 1702 1965
Size: 163 99 822 107 402 109 263
Fragments arranged by size:
822 402 263 163 109 107 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 827 1965
Size: 827 1138
SmaI CCC'GGG
Cuts at: 0 592 603 895 1965
Size: 592 11 292 1070
Fragments arranged by size:
1070 592 292 11
SmlI C'TyrA_G
Cuts at: 0 1318 1965
Size: 1318 647
SphI G_CATG'C
Cuts at: 0 1390 1834 1965
Size: 1390 444 131
Fragments arranged by size:
1390 444 131
SrfI GCCC'GGGC
Cuts at: 0 592 1965
Size: 592 1373
StuI AGG'CCT
Cuts at: 0 979 1030 1965
Size: 979 51 935
Fragments arranged by size:
979 935 51
StyI C'CwwG_G
Cuts at: 0 452 823 1965
Size: 452 371 1142
Fragments arranged by size:
1142 452 371
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 1603 1906 1965
Size: 454 1149 303 59
Fragments arranged by size:
1149 454 303 59
TatI w'GTAC_w
Cuts at: 0 8 632 971 1148 1965
Size: 8 624 339 177 817
Fragments arranged by size:
817 624 339 177 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 1063 1095 1618 1650 1831 1863
Size: 40 32 991 32 523 32 181 32
Cuts at: 1863 1965
Size: 102
Fragments arranged by size:
991 523 181 102 40 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 697 1516 1965
Size: 697 819 449
Fragments arranged by size:
819 697 449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1248 1935 1965
Size: 1248 687 30
Fragments arranged by size:
1248 687 30
XmaI C'CCGG_G
Cuts at: 0 590 601 893 1965
Size: 590 11 292 1072
Fragments arranged by size:
1072 590 292 11
ZraI GAC'GTC
Cuts at: 0 1164 1515 1965
Size: 1164 351 450
Fragments arranged by size:
1164 450 351
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BglI Bme1580I
BmgBI BmtI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstXI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII
HindIII MslI MspA1I NaeI NgoMIV NheI NspI PflMI
PfoI PpuMI PspOMI PsrI PstI PvuII SacI SalI
SanDI SbfI SfcI SfiI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BplI
Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI EcoRV FalI HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SacII SapI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI