Restriction map for
pF1KE2495
(Linear) (Six-base) MAPSORT of: KIEE2495.seq Check: 4942 from: 1 to: 5817
KIEE2495.orf
With 182 enzymes: SgfI *
February 14, 2014 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1248 3084 3288 5817
Size: 1248 1836 204 2529
Fragments arranged by size:
2529 1836 1248 204
AatII G_ACGT'C
Cuts at: 0 3698 5817
Size: 3698 2119
AccI GT'mk_AC
Cuts at: 0 1049 5817
Size: 1049 4768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 2545 3019 3202 3349 4504 4975 5026
Size: 1013 1532 474 183 147 1155 471 51
Cuts at: 5026 5332 5644 5817
Size: 306 312 173
Fragments arranged by size:
1532 1155 1013 474 471 312 306 183
173 147 51
AfeI AGC'GCT
Cuts at: 0 322 2716 4306 5817
Size: 322 2394 1590 1511
Fragments arranged by size:
2394 1590 1511 322
AflII C'TTAA_G
Cuts at: 0 3244 5817
Size: 3244 2573
AflIII A'CryG_T
Cuts at: 0 1478 5817
Size: 1478 4339
AleI CACnn'nnGTG
Cuts at: 0 2290 3478 5817
Size: 2290 1188 2339
Fragments arranged by size:
2339 2290 1188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1567 2113 5059 5817
Size: 1567 546 2946 758
Fragments arranged by size:
2946 1567 758 546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 706 738 1603 1635 5817
Size: 706 32 865 32 4182
Fragments arranged by size:
4182 865 706 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 870 3024 3303 3654 4194 5121 5151 5448
Size: 870 2154 279 351 540 927 30 297
Cuts at: 5448 5604 5817
Size: 156 213
Fragments arranged by size:
2154 927 870 540 351 297 279 213
156 30
ApaI G_GGCC'C
Cuts at: 0 4689 5817
Size: 4689 1128
ApoI r'AATT_y
Cuts at: 0 739 5817
Size: 739 5078
AseI AT'TA_AT
Cuts at: 0 3266 5817
Size: 3266 2551
AvaI C'yCGr_G
Cuts at: 0 158 351 815 1208 2381 3425 3911 5817
Size: 158 193 464 393 1173 1044 486 1906
Fragments arranged by size:
1906 1173 1044 486 464 393 193 158
BamHI G'GATC_C
Cuts at: 0 749 1797 3112 5817
Size: 749 1048 1315 2705
Fragments arranged by size:
2705 1315 1048 749
BanI G'GyrC_C
Cuts at: 0 643 1132 5788 5817
Size: 643 489 4656 29
Fragments arranged by size:
4656 643 489 29
BanII G_rGCy'C
Cuts at: 0 296 989 1281 1930 2813 2855 4163 4448
Size: 296 693 292 649 883 42 1308 285
Cuts at: 4448 4689 5555 5817
Size: 241 866 262
Fragments arranged by size:
1308 883 866 693 649 296 292 285
262 241 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 205 556 2843 3053 4258 5579 5817
Size: 205 351 2287 210 1205 1321 238
Fragments arranged by size:
2287 1321 1205 351 238 210 205
BbvCI CC'TCA_GC
Cuts at: 0 2708 4435 5817
Size: 2708 1727 1382
Fragments arranged by size:
2708 1727 1382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2152 2186 3550 3584 3989 4023 5817
Size: 2152 34 1364 34 405 34 1794
Fragments arranged by size:
2152 1794 1364 405 34 34 34
BclI T'GATC_A
Cuts at: 0 2259 2729 5817
Size: 2259 470 3088
Fragments arranged by size:
3088 2259 470
BglI GCCn_nnn'nGGC
Cuts at: 0 16 3469 4210 4219 5275 5299 5656 5817
Size: 16 3453 741 9 1056 24 357 161
Fragments arranged by size:
3453 1056 741 357 161 24 16 9
BglII A'GATC_T
Cuts at: 0 1400 5817
Size: 1400 4417
BlpI GC'TnA_GC
Cuts at: 0 2242 2347 2975 4891 5054 5817
Size: 2242 105 628 1916 163 763
Fragments arranged by size:
2242 1916 763 628 163 105
Bme1580I G_kGCm'C
Cuts at: 0 119 646 4689 5817
Size: 119 527 4043 1128
Fragments arranged by size:
4043 1128 527 119
BmgBI CAC'GTC
Cuts at: 0 1014 3476 5817
Size: 1014 2462 2341
Fragments arranged by size:
2462 2341 1014
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 1898 2179 4107 5817
Size: 33 1865 281 1928 1710
Fragments arranged by size:
1928 1865 1710 281 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4446 4548 5817
Size: 4446 102 1269
Fragments arranged by size:
4446 1269 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 703 826 1363 1510 1624 2030 2122
Size: 670 33 123 537 147 114 406 92
Cuts at: 2122 2344 2614 2644 2770 2803 2896 3106 3127
Size: 222 270 30 126 33 93 210 21
Cuts at: 3127 3169 3190 3379 3391 3454 3559 3676 3703
Size: 42 21 189 12 63 105 117 27
Cuts at: 3703 3760 3781 3982 4267 4426 4501 4603 4624
Size: 57 21 201 285 159 75 102 21
Cuts at: 4624 4639 4657 4678 4927 5080 5200 5362 5491
Size: 15 18 21 249 153 120 162 129
Cuts at: 5491 5503 5817
Size: 12 314
Fragments arranged by size:
670 537 406 314 285 270 249 222
210 201 189 162 159 153 147 129
126 123 120 117 114 105 102 93
92 75 63 57 42 33 33 30
27 21 21 21 21 21 18 15
12 12
Bpu10I CC'TnA_GC
Cuts at: 0 2708 3395 4435 5817
Size: 2708 687 1040 1382
Fragments arranged by size:
2708 1382 1040 687
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1174 1828 2839 4441 5404 5817
Size: 298 876 654 1011 1602 963 413
Fragments arranged by size:
1602 1011 963 876 654 413 298
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1339 4486 4859 5122 5817
Size: 787 552 3147 373 263 695
Fragments arranged by size:
3147 787 695 552 373 263
BsaBI GATnn'nnATC
Cuts at: 0 4882 5817
Size: 4882 935
BsaHI Gr'CG_yC
Cuts at: 0 1046 3695 4139 5817
Size: 1046 2649 444 1678
Fragments arranged by size:
2649 1678 1046 444
BsaWI w'CCGG_w
Cuts at: 0 710 5817
Size: 710 5107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 188 448 478 499 529 706 709
Size: 158 30 260 30 21 30 177 3
Cuts at: 709 736 739 1597 1603 1627 1633 3685 3715
Size: 27 3 858 6 24 6 2052 30
Cuts at: 3715 3733 3763 4381 4411 4612 4642 5817
Size: 18 30 618 30 201 30 1175
Fragments arranged by size:
2052 1175 858 618 260 201 177 158
30 30 30 30 30 30 30 27
24 21 18 6 6 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 453 714 997 1048 1126 1513 1621 1624
Size: 453 261 283 51 78 387 108 3
Cuts at: 1624 1857 2341 2641 2725 2806 2809 2959 3367
Size: 233 484 300 84 81 3 150 408
Cuts at: 3367 3376 3379 3451 3556 3979 3982 4063 4066
Size: 9 3 72 105 423 3 81 3
Cuts at: 4066 4171 4222 4230 4402 4486 4654 4681 4762
Size: 105 51 8 172 84 168 27 81
Cuts at: 4762 5077 5245 5275 5320 5668 5677 5740 5817
Size: 315 168 30 45 348 9 63 77
Fragments arranged by size:
484 453 423 408 387 348 315 300
283 261 233 172 168 168 150 108
105 105 84 84 81 81 81 78
77 72 63 51 51 45 30 27
9 9 8 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1017 2953 3612 3830 3932 4187 4688 5210
Size: 1017 1936 659 218 102 255 501 522
Cuts at: 5210 5817
Size: 607
Fragments arranged by size:
1936 1017 659 607 522 501 255 218
102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1273 2698 3502 5164 5260 5268 5722
Size: 271 1002 1425 804 1662 96 8 454
Cuts at: 5722 5817
Size: 95
Fragments arranged by size:
1662 1425 1002 804 454 271 96 95
8
BsiHKAI G_wGCw'C
Cuts at: 0 989 2813 2848 2855 4163 4240 4448 4487
Size: 989 1824 35 7 1308 77 208 39
Cuts at: 4487 4664 5555 5817
Size: 177 891 262
Fragments arranged by size:
1824 1308 989 891 262 208 177 77
39 35 7
BsmI GAATG_Cn'
Cuts at: 0 5154 5817
Size: 5154 663
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2530 2581 4126 5817
Size: 2530 51 1545 1691
Fragments arranged by size:
2530 1691 1545 51
Bsp1286I G_dGCh'C
Cuts at: 0 119 296 646 989 1281 1930 2813 2848
Size: 119 177 350 343 292 649 883 35
Cuts at: 2848 2855 4163 4240 4448 4487 4664 4689 5555
Size: 7 1308 77 208 39 177 25 866
Cuts at: 5555 5817
Size: 262
Fragments arranged by size:
1308 883 866 649 350 343 292 262
208 177 177 119 77 39 35 25
7
BspEI T'CCGG_A
Cuts at: 0 710 5817
Size: 710 5107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 809 1193 1248 1559 2672 3084 3288 3641
Size: 809 384 55 311 1113 412 204 353
Cuts at: 3641 4037 5051 5390 5594 5817
Size: 396 1014 339 204 223
Fragments arranged by size:
1113 1014 809 412 396 384 353 339
311 223 204 204 55
BsrBI CCG'CTC
Cuts at: 0 679 2037 3450 4725 5031 5094 5139 5538
Size: 679 1358 1413 1275 306 63 45 399
Cuts at: 5538 5730 5817
Size: 192 87
Fragments arranged by size:
1413 1358 1275 679 399 306 192 87
63 45
BsrDI GCAATG_nn'
Cuts at: 0 176 590 702 2097 5817
Size: 176 414 112 1395 3720
Fragments arranged by size:
3720 1395 414 176 112
BsrFI r'CCGG_y
Cuts at: 0 393 1750 3463 4262 5390 5817
Size: 393 1357 1713 799 1128 427
Fragments arranged by size:
1713 1357 1128 799 427 393
BsrGI T'GTAC_A
Cuts at: 0 398 5817
Size: 398 5419
BssSI C'ACGA_G
Cuts at: 0 289 3559 4663 5817
Size: 289 3270 1104 1154
Fragments arranged by size:
3270 1154 1104 289
BstAPI GCAn_nnn'nTGC
Cuts at: 0 107 1251 2082 5151 5802 5817
Size: 107 1144 831 3069 651 15
Fragments arranged by size:
3069 1144 831 651 107 15
BstEII G'GTnAC_C
Cuts at: 0 604 909 5817
Size: 604 305 4908
Fragments arranged by size:
4908 604 305
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4908 5817
Size: 4908 909
BstYI r'GATC_y
Cuts at: 0 749 1400 1797 3112 4358 4676 4883 4967
Size: 749 651 397 1315 1246 318 207 84
Cuts at: 4967 5202 5817
Size: 235 615
Fragments arranged by size:
1315 1246 749 651 615 397 318 235
207 84
Bsu36I CC'TnA_GG
Cuts at: 0 1990 2198 4840 5817
Size: 1990 208 2642 977
Fragments arranged by size:
2642 1990 977 208
BtgI C'CryG_G
Cuts at: 0 296 609 959 1583 3572 4214 5075 5817
Size: 296 313 350 624 1989 642 861 742
Fragments arranged by size:
1989 861 742 642 624 350 313 296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1371 2955 5001 5724 5817
Size: 1371 1584 2046 723 93
Fragments arranged by size:
2046 1584 1371 723 93
BtsI GCAGTG_nn'
Cuts at: 0 1298 2813 3566 4000 5243 5817
Size: 1298 1515 753 434 1243 574
Fragments arranged by size:
1515 1298 1243 753 574 434
DraIII CAC_nnn'GTG
Cuts at: 0 107 2013 5817
Size: 107 1906 3804
Fragments arranged by size:
3804 1906 107
DrdI GACnn_nn'nnGTC
Cuts at: 0 4984 5094 5817
Size: 4984 110 723
Fragments arranged by size:
4984 723 110
EaeI y'GGCC_r
Cuts at: 0 269 416 992 1283 1853 2189 2280 2552
Size: 269 147 576 291 570 336 91 272
Cuts at: 2552 2888 3605 4364 5817
Size: 336 717 759 1453
Fragments arranged by size:
1453 759 717 576 570 336 336 291
272 269 147 91
EarI CTCTTCn'nnn_
Cuts at: 0 125 364 430 1867 2521 2561 3452 3661
Size: 125 239 66 1437 654 40 891 209
Cuts at: 3661 3791 4160 4453 5524 5817
Size: 130 369 293 1071 293
Fragments arranged by size:
1437 1071 891 654 369 293 293 239
209 130 125 66 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1135 2698 5125 5674 5749 5817
Size: 1135 1563 2427 549 75 68
Fragments arranged by size:
2427 1563 1135 549 75 68
EcoICRI GAG'CTC
Cuts at: 0 987 2811 2853 4161 4446 5553 5817
Size: 987 1824 42 1308 285 1107 264
Fragments arranged by size:
1824 1308 1107 987 285 264 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 703 826 1013 1363 1510 1624 2030
Size: 670 33 123 187 350 147 114 406
Cuts at: 2030 2122 2344 2545 2614 2644 2770 2803 2896
Size: 92 222 201 69 30 126 33 93
Cuts at: 2896 3019 3106 3127 3169 3190 3202 3349 3379
Size: 123 87 21 42 21 12 147 30
Cuts at: 3379 3391 3454 3559 3676 3703 3760 3781 3982
Size: 12 63 105 117 27 57 21 201
Cuts at: 3982 4267 4426 4501 4504 4603 4624 4639 4657
Size: 285 159 75 3 99 21 15 18
Cuts at: 4657 4678 4927 4975 5026 5080 5200 5332 5362
Size: 21 249 48 51 54 120 132 30
Cuts at: 5362 5491 5503 5644 5817
Size: 129 12 141 173
Fragments arranged by size:
670 406 350 285 249 222 201 201
187 173 159 147 147 141 132 129
126 123 123 120 117 114 105 99
93 92 87 75 69 63 57 54
51 48 42 33 33 30 30 30
27 21 21 21 21 21 18 15
12 12 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1189 1606 2647 2941 3541 4090 4522 4639
Size: 1189 417 1041 294 600 549 432 117
Cuts at: 4639 5047 5817
Size: 408 770
Fragments arranged by size:
1189 1041 770 600 549 432 417 408
294 117
EcoO109I rG'GnC_Cy
Cuts at: 0 447 3021 3534 3945 4836 5379 5601 5817
Size: 447 2574 513 411 891 543 222 216
Fragments arranged by size:
2574 891 543 513 447 411 222 216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2313 2520 3259 5817
Size: 2313 207 739 2558
Fragments arranged by size:
2558 2313 739 207
FspI TGC'GCA
Cuts at: 0 2398 3139 3583 5817
Size: 2398 741 444 2234
Fragments arranged by size:
2398 2234 741 444
FspAI rTGC'GCAy
Cuts at: 0 2398 3139 5817
Size: 2398 741 2678
Fragments arranged by size:
2678 2398 741
HaeII r_GCGC'y
Cuts at: 0 324 2718 3895 4308 5336 5646 5817
Size: 324 2394 1177 413 1028 310 171
Fragments arranged by size:
2394 1177 1028 413 324 310 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 499 531 709 741 1986 2018
Size: 158 32 309 32 178 32 1245 32
Cuts at: 2018 2830 2862 3685 3717 3733 3765 4381 4393
Size: 812 32 823 32 16 32 616 12
Cuts at: 4393 4413 4425 4612 4644 5817
Size: 20 12 187 32 1173
Fragments arranged by size:
1245 1173 823 812 616 309 187 178
158 32 32 32 32 32 32 32
32 20 16 12 12
HincII GTy'rAC
Cuts at: 0 375 3057 5757 5817
Size: 375 2682 2700 60
Fragments arranged by size:
2700 2682 375 60
HindIII A'AGCT_T
Cuts at: 0 3241 5761 5817
Size: 3241 2520 56
Fragments arranged by size:
3241 2520 56
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 899 1706 3728 5180 5201 5817
Size: 497 402 807 2022 1452 21 616
Fragments arranged by size:
2022 1452 807 616 497 402 21
MscI TGG'CCA
Cuts at: 0 271 994 1285 1855 2191 2282 2554 2890
Size: 271 723 291 570 336 91 272 336
Cuts at: 2890 5817
Size: 2927
Fragments arranged by size:
2927 723 570 336 336 291 272 271
91
MslI CAynn'nnrTG
Cuts at: 0 271 1483 1636 2290 2365 3478 5817
Size: 271 1212 153 654 75 1113 2339
Fragments arranged by size:
2339 1212 1113 654 271 153 75
MspA1I CmG'CkG
Cuts at: 0 34 298 831 1107 1180 2082 2520 2727
Size: 34 264 533 276 73 902 438 207
Cuts at: 2727 2748 3231 3574 3639 3906 3960 4149 4191
Size: 21 483 343 65 267 54 189 42
Cuts at: 4191 4602 4779 4974 5148 5439 5817
Size: 411 177 195 174 291 378
Fragments arranged by size:
902 533 483 438 411 378 343 291
276 267 264 207 195 189 177 174
73 65 54 42 34 21
NaeI GCC'GGC
Cuts at: 0 1752 3465 5817
Size: 1752 1713 2352
Fragments arranged by size:
2352 1752 1713
NcoI C'CATG_G
Cuts at: 0 1583 5817
Size: 1583 4234
NgoMIV G'CCGG_C
Cuts at: 0 1750 3463 5817
Size: 1750 1713 2354
Fragments arranged by size:
2354 1750 1713
NspI r_CATG'y
Cuts at: 0 495 903 1482 1620 2370 2914 5817
Size: 495 408 579 138 750 544 2903
Fragments arranged by size:
2903 750 579 544 495 408 138
PciI A'CATG_T
Cuts at: 0 1478 5817
Size: 1478 4339
PflMI CCAn_nnn'nTGG
Cuts at: 0 3939 4782 5817
Size: 3939 843 1035
Fragments arranged by size:
3939 1035 843
PfoI T'CCnGG_A
Cuts at: 0 1379 1601 5098 5817
Size: 1379 222 3497 719
Fragments arranged by size:
3497 1379 719 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 706 738 1291 1323 1603 1635 2191 2223
Size: 706 32 553 32 280 32 556 32
Cuts at: 2223 3499 3531 5817
Size: 1276 32 2286
Fragments arranged by size:
2286 1276 706 556 553 280 32 32
32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3534 3945 4836 5379 5601 5817
Size: 3534 411 891 543 222 216
Fragments arranged by size:
3534 891 543 411 222 216
PspOMI G'GGCC_C
Cuts at: 0 4685 5817
Size: 4685 1132
PstI C_TGCA'G
Cuts at: 0 786 1460 1556 2122 2651 2669 3230 3557
Size: 786 674 96 566 529 18 561 327
Cuts at: 3557 3638 3848 4007 4079 4199 4326 4628 4799
Size: 81 210 159 72 120 127 302 171
Cuts at: 4799 5051 5387 5444 5817
Size: 252 336 57 373
Fragments arranged by size:
786 674 566 561 529 373 336 327
302 252 210 171 159 127 120 96
81 72 57 18
PvuII CAG'CTG
Cuts at: 0 831 1180 2082 2727 2748 3231 3906 3960
Size: 831 349 902 645 21 483 675 54
Cuts at: 3960 4602 4974 5148 5439 5817
Size: 642 372 174 291 378
Fragments arranged by size:
902 831 675 645 642 483 378 372
349 291 174 54 21
RsrII CG'GwC_CG
Cuts at: 0 4986 5817
Size: 4986 831
SacI G_AGCT'C
Cuts at: 0 989 2813 2855 4163 4448 5555 5817
Size: 989 1824 42 1308 285 1107 262
Fragments arranged by size:
1824 1308 1107 989 285 262 42
SacII CC_GC'GG
Cuts at: 0 299 3575 5817
Size: 299 3276 2242
Fragments arranged by size:
3276 2242 299
SapI GCTCTTCn'nnn_
Cuts at: 0 2521 3661 4453 5524 5817
Size: 2521 1140 792 1071 293
Fragments arranged by size:
2521 1140 1071 792 293
SbfI CC_TGCA'GG
Cuts at: 0 1556 5817
Size: 1556 4261
ScaI AGT'ACT
Cuts at: 0 580 1366 5817
Size: 580 786 4451
Fragments arranged by size:
4451 786 580
SexAI A'CCwGG_T
Cuts at: 0 221 5603 5817
Size: 221 5382 214
Fragments arranged by size:
5382 221 214
SfcI C'TryA_G
Cuts at: 0 782 1456 1552 2118 2647 2665 3226 3316
Size: 782 674 96 566 529 18 561 90
Cuts at: 3316 3553 3634 3844 4003 4075 4195 4267 4322
Size: 237 81 210 159 72 120 72 55
Cuts at: 4322 4624 4795 5047 5383 5440 5596 5817
Size: 302 171 252 336 57 156 221
Fragments arranged by size:
782 674 566 561 529 336 302 252
237 221 210 171 159 156 120 96
90 81 72 72 57 55 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3469 5275 5817
Size: 3469 1806 542
Fragments arranged by size:
3469 1806 542
SmaI CCC'GGG
Cuts at: 0 160 817 2383 3913 5817
Size: 160 657 1566 1530 1904
Fragments arranged by size:
1904 1566 1530 657 160
SmlI C'TyrA_G
Cuts at: 0 313 1189 1843 2854 3244 4456 5419 5817
Size: 313 876 654 1011 390 1212 963 398
Fragments arranged by size:
1212 1011 963 876 654 398 390 313
SphI G_CATG'C
Cuts at: 0 2370 2914 5817
Size: 2370 544 2903
Fragments arranged by size:
2903 2370 544
StuI AGG'CCT
Cuts at: 0 688 1558 4651 5635 5817
Size: 688 870 3093 984 182
Fragments arranged by size:
3093 984 870 688 182
StyI C'CwwG_G
Cuts at: 0 1238 1286 1583 1625 2288 2891 2984 3728
Size: 1238 48 297 42 663 603 93 744
Cuts at: 3728 4064 4091 5817
Size: 336 27 1726
Fragments arranged by size:
1726 1238 744 663 603 336 297 93
48 42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 258 2014 3844 3865 4978 5817
Size: 19 239 1756 1830 21 1113 839
Fragments arranged by size:
1830 1756 1113 839 239 21 19
TatI w'GTAC_w
Cuts at: 0 398 488 578 1262 1364 1502 2270 5817
Size: 398 90 90 684 102 138 768 3547
Fragments arranged by size:
3547 768 684 398 138 102 90 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 448 480 903 935 1597 1629 2827 2859
Size: 448 32 423 32 662 32 1198 32
Cuts at: 2859 4556 4588 5817
Size: 1697 32 1229
Fragments arranged by size:
1697 1229 1198 662 448 423 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1944 3052 5817
Size: 1944 1108 2765
Fragments arranged by size:
2765 1944 1108
XbaI T'CTAG_A
Cuts at: 0 69 5817
Size: 69 5748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 771 1278 2275 3396 3735 4104 4179
Size: 678 93 507 997 1121 339 369 75
Cuts at: 4179 5337 5817
Size: 1158 480
Fragments arranged by size:
1158 1121 997 678 507 480 369 339
93 75
XmaI C'CCGG_G
Cuts at: 0 158 815 2381 3911 5817
Size: 158 657 1566 1530 1906
Fragments arranged by size:
1906 1566 1530 657 158
XmnI GAAnn'nnTTC
Cuts at: 0 1306 2128 5817
Size: 1306 822 3689
Fragments arranged by size:
3689 1306 822
ZraI GAC'GTC
Cuts at: 0 3696 5817
Size: 3696 2121
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApoI AseI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SapI SbfI
ScaI SexAI SfcI SfiI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AhdI ApaLI AscI AsiSI AvrII
BaeI BbeI BciVI BfrBI BmtI BsaAI BsiEI BsiWI
BspHI BssHII BstBI BstZ17I ClaI CspCI DraI EagI
EcoRI EcoRV FseI HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI SalI
SanDI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XhoI