Restriction map for
pF1KE2494
(Linear) (Six-base) MAPSORT of: KIEE2494.seq Check: 7934 from: 1 to: 1170
KIEE2494.orf
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AccI GT'mk_AC
Cuts at: 0 308 1170
Size: 308 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 673 802 1170
Size: 374 299 129 368
Fragments arranged by size:
374 368 299 129
AleI CACnn'nnGTG
Cuts at: 0 427 1170
Size: 427 743
ApaI G_GGCC'C
Cuts at: 0 8 1170
Size: 8 1162
AvaI C'yCGr_G
Cuts at: 0 205 1170
Size: 205 965
BanII G_rGCy'C
Cuts at: 0 8 1170
Size: 8 1162
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 991 1170
Size: 287 704 179
Fragments arranged by size:
704 287 179
BbvCI CC'TCA_GC
Cuts at: 0 584 1091 1170
Size: 584 507 79
Fragments arranged by size:
584 507 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 431 1170
Size: 397 34 739
Fragments arranged by size:
739 397 34
BclI T'GATC_A
Cuts at: 0 128 1170
Size: 128 1042
BglII A'GATC_T
Cuts at: 0 924 1170
Size: 924 246
Bme1580I G_kGCm'C
Cuts at: 0 8 1170
Size: 8 1162
BmrI ACTGGGnnnn_n'
Cuts at: 0 987 1170
Size: 987 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1120 1132 1144 1170
Size: 277 843 12 12 26
Fragments arranged by size:
843 277 26 12 12
Bpu10I CC'TnA_GC
Cuts at: 0 584 1091 1170
Size: 584 507 79
Fragments arranged by size:
584 507 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1170
Size: 874 296
BsaI GGTCTCn'nnnn_
Cuts at: 0 796 1170
Size: 796 374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 374 404 781 811 973 1003 1170
Size: 374 30 377 30 162 30 167
Fragments arranged by size:
377 374 167 162 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 259 601 745 766 1087 1108 1170
Size: 178 81 342 144 21 321 21 62
Fragments arranged by size:
342 321 178 144 81 62 21 21
BsiHKAI G_wGCw'C
Cuts at: 0 758 1170
Size: 758 412
Bsp1286I G_dGCh'C
Cuts at: 0 8 758 1170
Size: 8 750 412
Fragments arranged by size:
750 412 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1088 1170
Size: 1088 82
BstYI r'GATC_y
Cuts at: 0 924 1170
Size: 924 246
Bsu36I CC'TnA_GG
Cuts at: 0 772 1170
Size: 772 398
BtsI GCAGTG_nn'
Cuts at: 0 34 1170
Size: 34 1136
DraIII CAC_nnn'GTG
Cuts at: 0 837 1170
Size: 837 333
DrdI GACnn_nn'nnGTC
Cuts at: 0 66 1170
Size: 66 1104
EaeI y'GGCC_r
Cuts at: 0 332 1170
Size: 332 838
EarI CTCTTCn'nnn_
Cuts at: 0 686 722 1170
Size: 686 36 448
Fragments arranged by size:
686 448 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 374 673 802 1120 1132 1144 1170
Size: 277 97 299 129 318 12 12 26
Fragments arranged by size:
318 299 277 129 97 26 12 12
EcoO109I rG'GnC_Cy
Cuts at: 0 4 456 1077 1170
Size: 4 452 621 93
Fragments arranged by size:
621 452 93 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 374 406 1170
Size: 205 32 137 32 764
Fragments arranged by size:
764 205 137 32 32
HincII GTy'rAC
Cuts at: 0 741 1170
Size: 741 429
MscI TGG'CCA
Cuts at: 0 334 1170
Size: 334 836
MslI CAynn'nnrTG
Cuts at: 0 427 1170
Size: 427 743
MspA1I CmG'CkG
Cuts at: 0 328 583 1170
Size: 328 255 587
Fragments arranged by size:
587 328 255
NspI r_CATG'y
Cuts at: 0 369 1170
Size: 369 801
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 1170
Size: 837 333
PfoI T'CCnGG_A
Cuts at: 0 254 1121 1170
Size: 254 867 49
Fragments arranged by size:
867 254 49
PpuMI rG'GwC_Cy
Cuts at: 0 456 1077 1170
Size: 456 621 93
Fragments arranged by size:
621 456 93
PspOMI G'GGCC_C
Cuts at: 0 4 1170
Size: 4 1166
PstI C_TGCA'G
Cuts at: 0 494 1170
Size: 494 676
PvuII CAG'CTG
Cuts at: 0 328 583 1170
Size: 328 255 587
Fragments arranged by size:
587 328 255
SapI GCTCTTCn'nnn_
Cuts at: 0 686 1170
Size: 686 484
SfcI C'TryA_G
Cuts at: 0 490 1170
Size: 490 680
SmlI C'TyrA_G
Cuts at: 0 889 1170
Size: 889 281
SspI AAT'ATT
Cuts at: 0 507 1170
Size: 507 663
StuI AGG'CCT
Cuts at: 0 220 347 682 1170
Size: 220 127 335 488
Fragments arranged by size:
488 335 220 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 1170
Size: 78 1092
XmnI GAAnn'nnTTC
Cuts at: 0 160 1170
Size: 160 1010
Enzymes that do cut:
AccI AcuI AleI ApaI AvaI BanII BbsI BbvCI
BcgI BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BsiHKAI Bsp1286I BspMI BstYI Bsu36I
BtsI DraIII DrdI EaeI EarI Eco57MI EcoO109I Hin4I
HincII MscI MslI MspA1I NspI PflMI PfoI PpuMI
PspOMI PstI PvuII SapI SfcI SmlI SspI StuI
TaqII XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BciVI BfrBI
BglI BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI